130 research outputs found
Immune cells and oxidative stress in the endotoxin tolerance mouse model
Sepsis is a systemic inflammatory response that can lead to tissue damage and death. In order to increase our understanding of sepsis, experimental models are needed that produce relevant immune and inflammatory responses during a septic event. We describe a lipopolysaccharide tolerance mouse model to characterize the cellular and molecular alterations of immune cells during sepsis. The model presents a typical lipopolysaccharide tolerance pattern in which tolerance is related to decreased production and secretion of cytokines after a subsequent exposure to a lethal dose of lipopolysaccharide. The initial lipopolysaccharide exposure also altered the expression patterns of cytokines and was followed by an 8- and a 1.5-fold increase in the T helper 1 and 2 cell subpopulations. Behavioral data indicate a decrease in spontaneous activity and an increase in body temperature following exposure to lipopolysaccharide. In contrast, tolerant animals maintained production of reactive oxygen species and nitric oxide when terminally challenged by cecal ligation and puncture (CLP). Survival study after CLP showed protection in tolerant compared to naive animals. Spleen mass increased in tolerant animals followed by increases of B lymphocytes and subpopulation Th1 cells. An increase in the number of stem cells was found in spleen and bone marrow. We also showed that administration of spleen or bone marrow cells from tolerant to naive animals transfers the acquired resistance status. In conclusion, lipopolysaccharide tolerance is a natural reprogramming of the immune system that increases the number of immune cells, particularly T helper 1 cells, and does not reduce oxidative stress.FAPESPCNP
Designing And Validating The Methodology For The Internet Assessment Of Fish Consumption At A University Setting
Assessing fish consumption is complex and involves several factors; however, the use of questionnaires in surveys and the use of the Internet as tool to collect data have been considered promising approaches. Therefore, the objective of this research was to design a data collection technique using a questionnaire to assess fish consumption by making it available on a specific home page on the Internet. A bibliographical survey or review was carried out to identify the features of the instrument, and therefore pre-tests were conducted with previous instruments, followed by the Focus Group technique. Specialists then performed an analysis and conducted an online pre-test. Multivariate data analysis was applied using the SmartPLS software. The results indicate that 1.966 participants belonging to the University of São Paulo (USP) community participated in the test, and after the exclusion of some variables, a statistically significant results were obtained. The final constructs comprised consumption, quality, and general characteristics. The instrument consisted of behavioral statements in a 5-point Likert scale and multiple-choice questions. The Cronbach's alpha reliability coefficient was 0.66 for general characteristics, 0.98 for quality, and 0.91 for consumption, which indicate good reliability of the instrument. In conclusion, the results proved that the Internet assessment is efficient. The instrument of analysis allowed us to better understand the process of buying and consuming fish in the country, and it can be used as base for further research.342315323Bargas-Avila, J.A., Lotscher, J., Orsini, S., Opwis, K., Intranet satisfaction questionnaire: Development and validation of a questionnaire to measure user satisfaction with the Intranet (2009) Computers In Human Behavior, 25 (6), pp. 1241-1250. , http://dx.doi.org/10.1016/j.chb.2009.05.014Batagelj, Z., Vehovar, V., Technical and methodological issues in surveys (1998) Proceedings of the 53° Annual Conference of the American Association For Public Opinion Research (AAPOR), , http://www.ris.org/ris98/stlouis, St. Louis. Retrieved fromBliven, B., Kaufman, S., Spertus, J.A., Electronic collection of health-related quality of life data: Validity, time, benefits and patient preference (2001) Quality Life Research, 10 (1), pp. 15-22. , http://dx.doi.org/10.1023/A:1016740312904Boeckner, L., Pullen, S.C.H., Walker, S.N., Abbott, G.W., Block, T., Use and realiability of Word Wide Web Version of the block health habits and history questionnaire with older rural women (2002) Journal of Nutrition Education and Behavior, 34, pp. S20-S24. , http://dx.doi.org/10.1016/S1499-4046(06)60307-2Cantrell, M.A., Lupinacci, P., Methodological issues in online data collection (2007) Journal of Advanced Nursing, 60 (5), pp. 544-549. , http://dx.doi.org/10.1111/j.1365-2648.2007.04448.xChang, L., Krosnick, J.A., Comparing oral interviewing with self-administered computerized questionnaires an experiment (2010) Public Opinion Quarterly, 74 (1), pp. 154-167. , http://dx.doi.org/10.1093/poq/nfp090Chin, W.W., The partial least squares approach to structural equation modeling (1998) Modern Methods For Business Research, pp. 295-336. , In G. A. Marcoulides (Ed.), Mahwah: Lawrence Erlbaum AssociatesCronk, B.C., West, J.L., Personality research on the Internet: A comparison of web based and traditional instruments in take-home and in-class settings (2002) Behavior Research Methods Instruments and Computers, 34 (2), pp. 177-180. , http://dx.doi.org/10.3758/BF03195440Falk, R.F., Miller, N.B., (1992) A Primer For Soft Modeling, , New York: Akron Presshttp://www.brasilfoodtrends.com.br/Brasil_Food_Trends/index.html, Federação das Industrias do Estado de São Paulo-FIESP. (2010), Brasil food trends, São Paulo: FIESP. Retrieved fromFerri, P., Cavalli, N., Costa, E., Mangiatordi, A., Mizzella, S., Pozzali, A., Scenini, F., Italian university students and digital technologies: Some results from a field research (2009) Communications In Computer and Information Science, 49 (3), pp. 357-365. , http://dx.doi.org/10.1007/978-3-642-04757-2_38(2009) The State of World Fisheries and Aquaculture (SOFIA), , Food and Agriculture Organization of the United Nations-FAO, Rome: FAOGefen, D., Straub, D.W., Boudreau, M.C., Structural equation modeling and regression: Guidelines for research practice (2009) Communications of the Association For Information Systems, 4 (1), pp. 1-77Hair Jr., J.F., Black, W.C., Babin, B.J., Anderson, R.E., Tatham, R.L., (2009) Análise Multivariada De Dados, , 6. ed.). Porto Alegre: BookmanHanning, R.M.D., Royall, J.E., Toews, L., Blashill, L., Wegener, J., Driezen, P., Web-based food behaviour questionnaire: Validation with grades six to eight students (2009) Canadian Journal of Dietetic Practice and Research, 70, pp. 172-178. , http://dx.doi.org/10.3148/70.4.2009.172Hanscom, B., Lurie, J.D., Homa, K., Weinstein, J., Computerized questionnaires and the quality of survey data (2002) Spine, 27 (6), pp. 1797-1801. , http://dx.doi.org/10.1097/00007632-200208150-00020Hershberger, S.L., Marcoulides, G.A., Parramore, M.M., Structural equation modeling: An introduction (2003) Structural Equation Modeling: Applications In Ecological and Evolutionary Biology, , http://dx.doi.org/10.1017/CBO9780511542138.002, In B. H. Pugesek, A. Tomer & A. V. Eye (Eds.), Cambridge: Cambridge University PressKlem, L., Structural equation modeling (2006) Reading and Understanding More Multivariate Statistics, pp. 227-260. , In L. G. Grimm & P. R. Yarnold (Eds.), Washington: American Psychological AssociationMalhotra, N., (2008) Pesquisa De Marketing: Uma Orientação Aplicada, , 5. ed.). Porto Alegre: BookmanMaruayama, G.M., (1998) Basic of Structural Equation Modeling, , Thousand Oaks: Sage PublicationsMatthys, C., Pynaert, I., de Keyzer, W., Henauw, S., Validity and reproducibility of an adolescent Web-based food frequency questionnaire (2007) Journal of the American Dietetic Association, 107 (4), pp. 605-610. , http://dx.doi.org/10.1016/j.jada.2007.01.005Mina, K., Fritschi, L., Knuiman, M., A valid semiquantitative food frequency questionnaire to measure fish consumption (2007) European Journal of Clinical Nutrition, 61, pp. 1023-1031. , http://dx.doi.org/10.1038/sj.ejcn.1602617Morgan, D., (1997) Focus Group As Qualitative Research, pp. 80-88. , London: Sage Publications. Qualitative Research Methods Series 16Mueller, R.O., Structural equation modeling: Back to basics (1997) Structural Equation Modeling, 4 (4), pp. 353-369. , http://dx.doi.org/10.1080/10705519709540081Oetterer, M., (2002) Industrialização Do Pescado Cultivado, , Guaíba: AgropecuáriaPasquali, L., (2003) Psicometria: Teoria Dos Testes Na Psicologia E Na Educação, , Petrópolis: VozesPerez, G., Zwicker, R., Fatores determinantes da adoção de sistemas de informação na área de saúde: Um estudo sobre o prontuário médico eletrônico (2010) Revista De Administração Mackenzie, 11 (1), pp. 174-200. , http://dx.doi.org/10.1590/S1678-69712010000100008Ribeiro, R.C.L., Panato, E., Silva, M.M.S., Rosado, L.E.F., Rosado, G.P., Validade relativa de um questionário de freqüência alimentar para utilização em adultos (2009) Revista De Nutrição, 22 (1), pp. 81-95. , http://dx.doi.org/10.1590/S1415-52732009000100008Rodrigues, D.S., Ramos, R.A.R., Mendes, J.F.G., A system to evaluate and monitor quality of life in a University Campus (2005) Proceedings of the 9th International Conference On Computers In Urban Planning and Urban Management (CUPUM), , http://repositorium.sdum.uminho.pt/bitstream/1822/5064/1/Rodrigues_CI_1_2005.pdf, London. Retrieved fromSantos, F.V., (2001) A Importância Da Atividade Física Na Qualidade De Vida Disseminada Numa Página Da Internet (Dissertação De Mestrado), , Universidade Federal de Santa Catarina, FlorianópolisSirot, V., Dumas, C., Leblanc, J.C., Margarit, I., Food and nutrient intakes of French frequent seafood consumers with regard to fish consumption recommendations: Results from the CALIPSO study (2011) British Journal of Nutrition, 105 (9), pp. 1369-1380. , http://dx.doi.org/10.1017/S0007114510005027Sonoda, D.Y., (2006) Demanda Por Pescado No Brasil Entre 2002 E 2003, , Tese de doutorado). Universidade de São Paulo, PiracicabaSoriano, J.M., Molto, J.C., Manes, J., Dietary intake and food pattern among university students (2000) Nutrition Research, 20 (9), pp. 1249-1258. , http://dx.doi.org/10.1016/S0271-5317(00)00217-7Stanton, J.M., Rogelberg, S.G., Using Internet/intranet web pages to collect organizational research data (2001) Organizational Research Methods, 4 (3), pp. 200-217. , http://dx.doi.org/10.1177/109442810143002Touvier, M., Kesse-Guyot, E., Méjean, C., Pollet, C., Malon, A., Castetbon, K., Hercberg, S., Comparison between an interactive web-based self-administered 24 h dietary record and an interview by a dietitian for large-scale epidemiological studies (2001) British Journal of Nutrition, 105 (7), pp. 1055-1064. , http://dx.doi.org/10.1017/S0007114510004617Trondsen, T., Scholderer, J., Lund, E., Lund, E., Eggen, A., Perceived barriers to consumption of fish among Norwegian women (2003) Appetite, 41 (3), pp. 301-314. , http://dx.doi.org/10.1016/S0195-6663(03)00108-9(2010) Anuário Estatístico, , http://sistemas3.usp.br/anuario, Universidade de São Paulo-USP, Brasil: USP. Retrieved fromVanderslice, J., Laflamme, D.M., Wynkoop-Simmons, K., Comparing fish consumption information collected via a telephone survey and a food frequency questionnaire (2008) Epidemiology, 19 (6), pp. S315Veiga, L., Gondim, S.M.G., A utilização de métodos qualitativos na ciência política e no marketing político (2001) Opinião Pública, 2 (1), pp. 1-15Vinholis, M.M.B., Azevedo, P.F., Segurança do alimento e rastreabilidade: O caso BSE (2002) RAE Eletrônica, 1 (2), pp. 1-19. , http://dx.doi.org/10.1590/S1676-56482002000200008Zwicker, R., Souza, C.A., Bido, D.S., (2008) Uma Revisão Do Modelo Do Grau De Informatização De Empresas: Novas Propostas De Estimação E Modelagem Usando PLS (Partial Least Squares), , In Anais do 32° Encontro da ANPAD, Rio de Janeiro. CD-RO
Report from the Instituto Evandro Chagas, FSEP, Brazilian ministery of health, Belem, Brazil : Oropouche virus outbreaks in Maranhao and Goias states, Brazil
Sodium reduction in traditional dry-cured pork belly using glasswort powder (Salicornia herbacea) as a partial NaCl replacer
Sodium chloride (NaCl) is a key ingredient in the processing of traditional dry-cured meat products by improving microbial safety, sensory attributes and technological properties. However, increasing concern about the consumption of sodium and health has been supporting the development of low-sodium meat products. Several strategies to reduce sodium in dry-cured meat product have been tested, although the followed approaches sometimes result in undesirable characteristics concerning flavor, texture and mouthfeel. The use of halophytic plants such as glasswort (Salicornia herbacea) in food matrices has been suggested as a novel strategy to reduce sodium content, due its salty flavor. The main aim of the present study is to produce traditional dry-cured pork bellies from the Bísaro breed using glasswort as a NaCl partial replacer, and compare it with dry-cured bellies salted either with NaCl or a mix of NaCl + KCl. Control bellies (BC) were salted with 100% of NaCl, the second formulation (BK) had 50% of NaCl and 50% of KCl, and the third formulation (BG) had 90% of NaCl and 10% of glasswort powder (GP). After production, the bellies were evaluated for aw, pH, CIELab coordinates, weight loss, proximal composition, TBARS, collagen and chloride contents, fatty acid profile and sensory attributes. The use of BG in dry-cured pork bellies did not affect processing indicators such as weight loss, aw and pH. Concerning CIELab, only the coordinates L* and hue angle from the external surface color of BG were statistically different from BC and BK. As expected, ash and NaCl contents differed from BG to the other two formulations. SFA and indexes AI and TI were lower, whereas the MUFA and h/H ratio were higher in BG than other treatments, leading to a product with a healthier lipid profile. The sensory evaluation revealed differences in appearance, taste and flavor among treatments, but did not indicate any negative effects of BG in the product attributes. This study reinforces the potential of BG as a natural sodium reducer for the production of traditional dry-cured pork belliesThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal)
for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020
and UIDP/00690/2020) and SusTEC (LA/P/0007/2021). Grants of I.F., A.L. and L.V. are due to
NORTE-01-0247-FEDER-072234. P.E.S.M. acknowledges postdoctoral fellowship support from the
Ministry of Science and Innovation (MCIN, Spain) “Juan de la Cierva” program (IJC2020-043358-I).info:eu-repo/semantics/publishedVersio
New entomological and virological data on the vectors of sylvatic yellow fever in Brazil
Institut Français de Recherche Scientifique pour le Développement en Coopération. Belém, PA, Brasil / Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.O presente trabalho enfatiza resultados recentes sobre eco-epidemiologia da febre amarela, obtidos em duas localidades ecologicamente muito diferentes: Barcarena (PA), situada na floresta amazônica densa, e a região de Campo Grande (MS), coberta de cerrado atravessado por florestas de galeria na região Centro-oeste. No primeiro local foi isolada uma amostra a partir de um lote de 6 Haemagogus janthinomys. Na região de Campo Grande, 2. 480 mosquitos antropófilos foram coletados, dos quais cerca de 40% eram vetores potenciais da FA. Essas espécies, classificadas por ordem decrescente de abundãncia, eram: Ae. scapularis, Sa. chloropterus, Hg. janthinomys, Hg. leucocelaenus, Hg. spegazinii e Sa. soperi. Quatro amostras de FA foram isolados a partir de Hg. janthinomys, uma de Sa. chloropterus (primeiro registro na América do Sul), e uma de Sa. soperi (primeiro registro). As taxas mínimas de infecção variaram segundo os locais coleta, mas foram sempre alta comparação com dados anteriores. A taxa média de sobrevivência diária do Hg. janthinomys é igual a 0,9635, permitindo estimar o seu valor na ocasião em que os caso humanos mais recentes foram provavelmente contaminados. deduziu-se que as epizootias foram muito intensas e mais ou menos simultâneas nos dois locais considerados. O maior problema a ser resolvido é o modo de re-introdução do vírus, ou sua sobreviv~encia, em cada região sob estudo
Modifications of arbovirus transmission in relation to construction of dams in Brazilian Amazonia
Дискусійні питання історії ЗУНР
У статті аналізуються питання, які все ще залишаються в історіографії Західноукраїнської Народної Республіки. Автор звертає увагу на контроверсійні точки зору щодо революційних подій, які відбулися 1 листопада 1918 р. у Львові, стосовно часу існування ЗУНР, Акту злуки УНР та ЗУНР, причин поразки Української Галицької армії тощо.The author of the article analyses the questions that are still disputable in modern
Ukrainian historiography. The author attracts our attention to the controversial points
of view concerning the type of revolutionary events which took place on the 1 st of
November 1918 in Lviv, concerning the time of WUPR existence, concerning the Act of
Unification of Western Ukrainian People’s Republic end Ukrainian People’s Republic ,
concerning the reasons of the defeat of Ukrainian army, etc
Application Of Exploratory Factor Analysis To Assess Fish Consumption In A University Community [aplicação De Análise Fatorial Exploratória Para Avaliar O Consumo De Pescado Em Uma Comunidade Universitária]
The objective of this research was to use the technique of Exploratory Factor Analysis (EFA) for the adequacy of a tool for the assessment of fish consumption and the characteristics involved in this process. Data were collected during a campaign to encourage fish consumption in Brazil with the voluntarily participation of members of a university community. An assessment instrument consisting of multiple-choice questions and a five-point Likert scale was designed and used to measure the importance of certain attributes that influence the choice and consumption of fish. This study sample was composed of of 224 individuals, the majority were women (65.6%). With regard to the frequency of fish consumption, 37.67% of the volunteers interviewed said they consume the product two or three times a month, and 29.6% once a week. The Exploratory Factor Analysis (EFA) was used to group the variables; the extraction was made using the principal components and the rotation using the Quartimax method. The results show clusters in two main constructs, quality and consumption with Cronbach Alpha coefficients of 0.75 and 0.69, respectively, indicating good internal consistency.33199106Alves, A.L.S., Padrão alimentar de mulheres adultas (2006) Revista De Saúde Pública, 40 (5), pp. 865-873. , PMid:17301909Anastasi, A., Urbina, S., (2000) Testagem Psicológica, p. 575. , 7. ed. Porto Alegre: ArtMedBlank, L., Community based lifestyle interventions: Changing behaviour and improving health (2007) Public Health Nutrition, 29 (3), pp. 236-245. , http://dx.doi.org/10.1093/pubmed/fdm041, PMid:17601764(2010), http://www.mpa.gov.br/mpa/seap/html/Comercializacao/semana_do_peixe_int2 009.html, BRASIL. Ministério da Pesca e Aquicultura. A campanha vai ressaltar os benefícios do consumo de pescado e o seu prepare MPA, 2010a. Disponível em: Acesso em: 25 out(2010) Consumo Per Capita Aparente De Pescado No Brasil 1996-2009 - O Brasileiro Está Comendo Mais Pescado, , http://www.mpa.gov.br/mpa/seap/Jonathan/mpa3/docs/folder%20consumo%20de% 20pescado%202009%202.pdf, BRASIL. Ministério da Pesca e Aquicultura, MPA, 2010b. Disponível em, Acesso em: 02 outBurger, J., Fishing, fish consumption, and awareness about warnings in a university community in central New Jersey in 2007, and comparisons with 2004 (2008) Environmental Research, 108 (1), pp. 107-116. , http://dx.doi.org/10.1016/j.envres.2008.05.003, PMid:18632098Cooper, D.R., Schindler, P.S., (2003) Métodos De Pesquisa Em Administração, p. 640. , 7. ed. Porto Alegre: BookmanDijk, H.V., Perceptions of health risks and benefits associated with fish consumption among Russian consumers (2011) Appetite, 56 (2), pp. 227-234. , http://dx.doi.org/10.1016/j.appet.2010.12.008, PMid:21147191Dillon, W.R., Goldstein, M., (1984) Multivariate Analysis: Methods and Applications, p. 608. , USA: John Wiley and sons, Inc(2009) The State of World Fisheries and Aquaculture (SOFIA): 2008, p. 196. , FOOD AND AGRICULTURE ORGANIZATION - FAO, FAOFilho, F.D.B., Silva Jr., J.A., Visão além do alcance: Uma introdução à análise fatorial (2010) Opinião Pública, 16 (1), pp. 160-185. , http://dx.doi.org/10.1590/S0104-62762010000100007Hair Jr., F., (2009) Análise Multivariada De Dados, p. 688. , 6. ed. Porto Alegre: BookmanHarris, C.A., Knight, S., Worosz, M.R., Shopping for food safety and the public trust: What supply chain stakeholders need to know about consumer attitudes (2006) Food Safety Magazine, pp. 52-59. , http://www.foodsafetymagazine.com/articleFF.asp?id=723&sub=sub1, Disponível em, Acesso em: 19 marHonkanen, P., Olsen, S.O., Verplanken, B., Intention to consume seafood - the importance of habit (2005) Appetite, 45 (2), pp. 161-168. , http://dx.doi.org/10.1016/j.appet.2005.04.005, PMid:16011859Johnson, R.A., Wichern, D.W., (2000) Métodos Mutivariados Aplicados De Análise De Dados, , México: International Thomson EditoresMaciel, E.S., Inquérito sobre o consumo de pescado em feira comemorativa da "Semana do Peixe" em cidade do interior de São Paulo (2009) Revista Ciência E Tecnologia - UNIMEP, 31 (16), pp. 59-68Malhotra, N., (2006) Pesquisa De Marketing Uma Orientação Aplicada, p. 720. , Porto Alegre: BookmanMichels, I.L., Prochmann, A.M., (2010) Estudo Das Cadeias Produtivas De Mato Grosso Do Sul: Piscicultura, p. 152. , http://pt.scribd.com/doc/14791838/Cadeia-Produtiva-Da-Piscicultura-Em-MS -2003, UFMS, 2002, Disponível, em, Acesso em: 08 novMunõz, M.A., Adherence to the Mediterranean diet is associated with better mental and physical health (2009) British Journal of Nutrition, 101 (12), pp. 1821-1827. , http://dx.doi.org/10.1017/S0007114508143598, PMid:19079848Oetterer, M., (2002) Industrialização Do Pescado Cultivado, p. 200. , Guaíba: Editora AgropecuáriaOlsen, S.O., Exploring the relationship between convenience and fish consumption: A cross-cultural study (2007) Appetite, 49 (1), pp. 84-91. , http://dx.doi.org/10.1016/j.appet.2006.12.002, PMid:17261344Pasquali, L., Extração dos fatores (2005) Análise Fatorial Para Pesquisadores, pp. 55-86. , PASQUALI, L. (Org.), LabPam, PMid:16760627Pieniak, Z., Verbeke, W., Scholderer, J., Health-related beliefs and consumer knowledge as determinants of fish consumption (2010) Journal of Human Nutrition and Dietetics, 23 (5), pp. 480-488. , http://dx.doi.org/10.1111/j.1365-277X.2010.01045.x, PMid:20831707Raspor, P., Faces of foods on the world of food systems (2006) Acta Alimentaria, 35, pp. 247-249Rortveit, A.W., Olsen, S.O., Combining the role of convenience and consideration set size in explaining fish consumption in Norway (2009) Appetite, 52 (2), pp. 313-317. , http://dx.doi.org/10.1016/j.appet.2008.11.001, PMid:19038300Schawb, A.J., (2010) Eletronic Classroom. 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