Interação Entre Xilanase, Glicose Oxidase E ácido Ascórbico Na Qualidade Tecnológica De Pão Elaborado Com Farinha Do Trigo Integral

This study aimed to verify the performance of xylanase and its interaction with oxidants agents (glucose oxidase and ascorbic acid) on the quality of whole wheat bread. The experiment was based on a central composite rotational design and the Response Surface Methodology was used to analyze the results. None of the xylanase, glucose oxidase or ascorbic acid concentrations within the studied range led to a significant difference in the specific volume. The highest moisture content and the lowest firmness values were reported in the bread with lower and intermediate levels of xylanase and larger amounts of glucose oxidase and ascorbic acid. This effect was observed mainly at the end of the storage period. A minimum amount of xylanase (from 33 to 63 EDX kg-1 flour) showed to be essential for obtaining best results. Levels of ascorbic acid above 63mg kg-1 and glucose oxidase above 91 SRU kg-1 proved to be necessary to offer the beneficial effect of xylanase. © 2016, Universidade Federal de Santa Maria. All rights reserved.46122249225

Análise dos efeitos da introduçāo de medidas de gestāo na pescaria de camarāo do Banco de Sofala

Biological effects of management measures introduced in the shrimp fishery from 1985 to 1990 are analysed as well as the results from simulation of different closed seasons. Observed changes in recruitment, total catch, biomass and average individual weight during 1985 to 1990 for Penaeus indicus and Hetapenaeus Monoceros are also analysed. Further studies on a stock-recruitment relationship are important due to decline of recruitment levels in this period. A three-month closed season starting in January is recommended, without increases in the monthly fishing effort. The TAC shall be fixed according to the recruitment level each year

Stages of behavioral change and positive perception of the environment towards physical activity among urban park users

The objectives of this study were to identify the stages of behavioral change (SBC) towards physical activity (PA) among users of urban parks, to determine the association between socio-demographic and economic variables of users of urban parks in relation to physical activity (PA), and to analyze environmental indicators of the park perceived positively for PA. A total of 218 users of an urban park, who filled out a questionnaire regarding socio-demographic data, SBC and perception of the existing environment, participated in the study. The most prevalent SBC were maintenance (56.6%) and action (21.1%). Most park users (78.7%) presented an active behavior (maintenance + action) towards PA, with this behavior being prevalent among middle-aged individuals (p<0.05). The beauty and geographic location of the park, technological and architectonic factors, some normative policies (public services to the users’ disposition), and values and attitudes were perceived positively for PA, regardless of whether the users regularly performed PA or not. The study suggests that most public park users present an active behavior towards PA, especially middle-aged individuals. In addition, the perception of environmental indicators is positive among physically inactive and active park users, irrespective of SBC

Solar vortex tubes : vortex dynamics in the solar atmosphere

In this work, a state-of-the-art vortex detection method, Instantaneous Vorticity Deviation, is applied to locate three-dimensional vortex tube boundaries in numerical simulations of solar photospheric magnetoconvection performed by the MURaM code. We detected three-dimensional vortices distributed along intergranular regions and displaying coned shapes that extend from the photosphere to the low chromosphere. Based on a well-defined vortex center and boundary, we were able to determine averaged radial profiles and thereby investigate the dynamics across the vortical flows at different height levels. The solar vortex tubes present nonuniform angular rotational velocity, and, at all height levels, there are eddy viscosity effects within the vortices, which slow down the plasma as it moves toward the center. The vortices impact the magnetic field as they help to intensify the magnetic field at the sinking points, and in turn, the magnetic field ends up playing an essential role in the vortex dynamics. The magnetic field was found to be especially important to the vorticity evolution. On the other hand, it is shown that, in general, kinematic vortices do not give rise to magnetic vortices unless their tangential velocities at different height levels are high enough to overcome the magnetic tension

Role of ¿1-pyrroline-5-carboxylate dehydrogenase supports mitochondrial metabolism and host-cell invasion of Trypanosoma cruzi

Proline is crucial for energizing critical events throughout the life cycle of Trypanosoma cruzi, the etiological agent of Chagas disease. The proline breakdown pathway consists of two oxidation steps, both of which produce reducing equivalents as follows: the conversion of proline to ¿1-pyrroline-5-carboxylate (P5C), and the subsequent conversion of P5C to glutamate. We have identified and characterized the ¿1-pyrroline-5-carboxy- late dehydrogenase from T. cruzi (TcP5CDH) and report here on how this enzyme contributes to a central metabolic pathway in this parasite. Size-exclusion chromatography, two-dimen- sional gel electrophoresis, and small angle x-ray scattering analysis of TcP5CDH revealed an oligomeric state composed of two subunits of six protomers. TcP5CDH was found to complement a yeast strain deficient in PUT2 activity, confirming the enzyme’s functional role; and the biochemical parameters (Km, kcat, and kcat/Km) of the recombinant TcP5CDH were determined, exhibiting values comparable with those from T. cruzi lysates. In addition, TcP5CDH exhibited mitochondrial staining during the main stages of the T. cruzi life cycle. mRNA and enzymatic activity levels indicated the up-regulation (6-fold change) of TcP5CDH during the infective stages of the parasite. The participation of P5C as an energy source was also demonstrated. Overall, we propose that this enzymatic step is crucial for the viability of both replicative and infective forms of T. cruzi

Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora

The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de GlicobiologiaUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros NaturaisUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUNIFESP, EPM, Depto. de BioquímicaSciEL

Relationship between childhood maltreatment and geriatric depression: the mediator effect of personality traits.

Childhood maltreatment is an important factor associated with adverse mental health outcomes including geriatric depression and the "big five" personality characteristics. The objective of this study was to evaluate a model where personality characteristics mediate the relationship between childhood maltreatment and geriatric depression. In this cross-sectional study, elderly subjects from socioeconomically disadvantaged neighborhoods of Porto Alegre, Brazil (n = 260) completed the Childhood Trauma Questionnaire (CTQ), NEO-Five Factor Inventory (NEO-FFI), and Mini International Neuropsychiatric Interview 5.0 (MINI plus). We used structural equation modeling (SEM) to evaluate the mediation hypothesis. The five personality factors (neuroticism, extraversion, agreeableness, openness, and conscientiousness) were related to childhood maltreatment and depression. Mediation analysis revealed that neuroticism and extraversion are complete mediators, agreeableness and conscientiousness are partial mediators, and openness is not a mediator. These findings support the hypothesis in which childhood maltreatment is associated with geriatric depression and mediated by personality factors. These results suggest that reducing the maladaptive personality trait in elderly people who suffered childhood maltreatment could prevent geriatric depression