Detection of maize yellow stripe tenui-like virus by ELISA and dot- blot tests in host plants and leafhopper vector in Egypt

Antisera to the nucleoprotein of maize yellow stripe tenui-like virus (MYSV) were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected maize, wheat, barley, oats, and the graminaceous weeds #Bromus wildenowii, #Cenchrus biflorus, #Dichanthium annulatum, #Digitaria sanguinalis, #Echinochloa colonum, #Setaria verticillata and #S. viridis. In maize leaves, differences in virus titer appeared to be correlated with leaf age and with MYSV symptom-types. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers (#Cicadulina chinai). (Résumé d'auteur

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Characterization and serology of the leafhopper-borne maize yellow stripe virus in Egypt

The nucleoprotein and non-capsid protein of maize yellow stripe virus (MYSV) were purified from naturally or experimentally infected maize or sorghum plants. In SDS-PAGE, the apparent molecular weight of the nucleoprotein was 35,6 KD, and that of the non-capsid protein was 14,7 KD. Similar to tenuiviruses, the nucleoprotein of MYSV was associated with fine filaments and the non-capsid protein formed typical needle-shaped crystals. Following a 2-day acquisition feeding period on MYSV-infected plants, vector leafhoppers (#Cicadulina chinai) remained highly infective for 21 days. Antisera to the nucleoprotein and non-capsid protein of MYSV were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected maize, wheat, barley, oat and the graminaceous weeds #Bromus wildenowii, #Cenchrus biflorus, #Dichanthium annulatum, #Digitaria sanguinalis, #Echinochloa colonum, #Setaria viridis and #S. verticillata. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers. (Résumé d'auteur

Characterization and serology of the leafhopper-borne maize yellow stripe virus in Egypt

The nucleoprotein and non-capsid protein of maize yellow stripe virus (MYSV) were purified from naturally or experimentally infected maize or sorghum plants. In SDS-PAGE, the apparent molecular weight of the nucleoprotein was 35,6 KD, and that of the non-capsid protein was 14,7 KD. Similar to tenuiviruses, the nucleoprotein of MYSV was associated with fine filaments and the non-capsid protein formed typical needle-shaped crystals. Following a 2-day acquisition feeding period on MYSV-infected plants, vector leafhoppers (#Cicadulina chinai) remained highly infective for 21 days. Antisera to the nucleoprotein and non-capsid protein of MYSV were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and direct antigen coating (DAC) ELISA were used to detect MYSV in naturally or experimentally infected maize, wheat, barley, oat and the graminaceous weeds #Bromus wildenowii, #Cenchrus biflorus, #Dichanthium annulatum, #Digitaria sanguinalis, #Echinochloa colonum, #Setaria viridis and #S. verticillata. Dot-blot and DAC-ELISA were used also to detect MYSV in naturally or experimentally infective leafhoppers. (Résumé d'auteur

Simone de Beauvoir, Brigitte Bardot, and Back-Translation: The Trajectory of Beauvoir\u27s Discourse on the ‘Eternal Feminine’

info:eu-repo/semantics/publishe