A Guide to Simple and Informative Binding Assays

The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail to measure the affinity of the reactants for each other. This essay describes simple methods to get the most out of valuable reagents in binding experiments

The potential to narrow uncertainty in projections of stratospheric ozone over the 21st century

Future stratospheric ozone concentrations will be determined both by changes in the concentration of ozone depleting substances (ODSs) and by changes in stratospheric and tropospheric climate, including those caused by changes in anthropogenic greenhouse gases (GHGs). Since future economic development pathways and resultant emissions of GHGs are uncertain, anthropogenic climate change could be a significant source of uncertainty for future projections of stratospheric ozone. In this pilot study, using an "ensemble of opportunity" of chemistry-climate model (CCM) simulations, the contribution of scenario uncertainty from different plausible emissions pathways for ODSs and GHGs to future ozone projections is quantified relative to the contribution from model uncertainty and internal variability of the chemistry-climate system. For both the global, annual mean ozone concentration and for ozone in specific geographical regions, differences between CCMs are the dominant source of uncertainty for the first two-thirds of the 21st century, up-to and after the time when ozone concentrations return to 1980 values. In the last third of the 21st century, dependent upon the set of greenhouse gas scenarios used, scenario uncertainty can be the dominant contributor. This result suggests that investment in chemistry-climate modelling is likely to continue to refine projections of stratospheric ozone and estimates of the return of stratospheric ozone concentrations to pre-1980 levels

Unsupervised Monocular Depth Estimation for Night-time Images using Adversarial Domain Feature Adaptation

In this paper, we look into the problem of estimating per-pixel depth maps from unconstrained RGB monocular night-time images which is a difficult task that has not been addressed adequately in the literature. The state-of-the-art day-time depth estimation methods fail miserably when tested with night-time images due to a large domain shift between them. The usual photo metric losses used for training these networks may not work for night-time images due to the absence of uniform lighting which is commonly present in day-time images, making it a difficult problem to solve. We propose to solve this problem by posing it as a domain adaptation problem where a network trained with day-time images is adapted to work for night-time images. Specifically, an encoder is trained to generate features from night-time images that are indistinguishable from those obtained from day-time images by using a PatchGAN-based adversarial discriminative learning method. Unlike the existing methods that directly adapt depth prediction (network output), we propose to adapt feature maps obtained from the encoder network so that a pre-trained day-time depth decoder can be directly used for predicting depth from these adapted features. Hence, the resulting method is termed as "Adversarial Domain Feature Adaptation (ADFA)" and its efficacy is demonstrated through experimentation on the challenging Oxford night driving dataset. Also, The modular encoder-decoder architecture for the proposed ADFA method allows us to use the encoder module as a feature extractor which can be used in many other applications. One such application is demonstrated where the features obtained from our adapted encoder network are shown to outperform other state-of-the-art methods in a visual place recognition problem, thereby, further establishing the usefulness and effectiveness of the proposed approach.Comment: ECCV 202

Functional approach to the electromagnetic response function: the Longitudinal Channel

In this paper we address the (charge) longitudinal electromagnetic response for a homogeneous system of nucleons interacting via meson exchanges in the functional framework. This approach warrants consistency if the calculation is carried on order-by-order in the mesonic loop expansion with RPA-dressed mesonic propagators. At the 1-loop order and considering pion, rho and omega exchanges we obtain a quenching of the response, in line with the experimental results.Comment: RevTeX, 18 figures available upon request - to be published in Physical Review

Correlation Effects in Nuclear Transparency

The Glauber approximation is used to calculate the contribution of nucleon correlations in high-energy $A(e,e'N)$ reactions. When the excitation energy of the residual nucleus is small, the increase of the nuclear transparency due to correlations between the struck nucleon and the other nucleons is mostly compensated by a decrease of the transparency due to the correlations between non detected nucleons. We derive Glauber model predictions for nuclear transparency for the differential cross section when nuclear shell level excitations are measured. The role of correlations in color transparency is briefly discussed.Comment: 24 pages revtex, 4 uuencoded PostScript Figures as separate fil

High-precision Studies of the 3^{\bf{3}}He(e,e′^{\bf{\prime}}p) Reaction at the Quasielastic Peak

Precision studies of the reaction $^{3}$He(e,e$^\prime$p) using the three-spectrometer facility at the Mainz microtron MAMI are presented. All data are for quasielastic kinematics at $|\vec{q} | =685$ MeV/c. Absolute cross sections were measured at three electron kinematics. For the measured missing momenta range from 10 to 165 MeV/c, no strength is observed for missing energies higher than 20 MeV. Distorted momentum distributions were extracted for the two-body breakup and the continuum. The longitudinal and transverse behavior was studied by measuring the cross section for three photon polarizations. The longitudinal and transverse nature of the cross sections is well described by a currently accepted and widely used prescription of the off-shell electron-nucleon cross-section. The results are compared to modern three-body calculations and to previous data.Comment: 4 pages, 3 figures. Submitted for publication in Phys. Rev. Let

Magnetic Field scaling of Relaxation curves in Small Particle Systems

We study the effects of the magnetic field on the relaxation of the magnetization of small monodomain non-interacting particles with random orientations and distribution of anisotropy constants. Starting from a master equation, we build up an expression for the time dependence of the magnetization which takes into account thermal activation only over barriers separating energy minima, which, in our model, can be computed exactly from analytical expressions. Numerical calculations of the relaxation curves for different distribution widths, and under different magnetic fields H and temperatures T, have been performed. We show how a \svar scaling of the curves, at different T and for a given H, can be carried out after proper normalization of the data to the equilibrium magnetization. The resulting master curves are shown to be closely related to what we call effective energy barrier distributions, which, in our model, can be computed exactly from analytical expressions. The concept of effective distribution serves us as a basis for finding a scaling variable to scale relaxation curves at different H and a given T, thus showing that the field dependence of energy barriers can be also extracted from relaxation measurements.Comment: 12 pages, 9 figures, submitted to Phys. Rev.

Quasielastic 12C(e,e'p) Reaction at High Momentum Transfer

We measured the 12C(e,e'p) cross section as a function of missing energy in parallel kinematics for (q,w) = (970 MeV/c, 330 MeV) and (990 MeV/c, 475 MeV). At w=475 MeV, at the maximum of the quasielastic peak, there is a large continuum (E_m > 50 MeV) cross section extending out to the deepest missing energy measured, amounting to almost 50% of the measured cross section. The ratio of data to DWIA calculation is 0.4 for both the p- and s-shells. At w=330 MeV, well below the maximum of the quasielastic peak, the continuum cross section is much smaller and the ratio of data to DWIA calculation is 0.85 for the p-shell and 1.0 for the s-shell. We infer that one or more mechanisms that increase with $\omega$ transform some of the single-nucleon-knockout into multinucleon knockout, decreasing the valence knockout cross section and increasing the continuum cross section.Comment: 14 pages, 7 figures, Revtex (multicol, prc and aps styles), to appear in Phys Rev

Longitudinal and Transverse Quasi-Elastic Response Functions of Light Nuclei

The $^3$He and $^4$He longitudinal and transverse response functions are determined from an analysis of the world data on quasi-elastic inclusive electron scattering. The corresponding Euclidean response functions are derived and compared to those calculated with Green's function Monte Carlo methods, using realistic interactions and currents. Large contributions associated with two-body currents are found, particularly in the $^4$He transverse response, in agreement with data. The contributions of two-body charge and current operators in the $^3$He, $^4$He, and $^6$Li response functions are also studied via sum-rule techniques. A semi-quantitative explanation for the observed systematics in the excess of transverse quasi-elastic strength, as function of mass number and momentum transfer, is provided. Finally, a number of model studies with simplified interactions, currents, and wave functions is carried out to elucidate the role played, in the full calculation, by tensor interactions and correlations.Comment: 40 pages, 11 figures, submitted to Phys. Rev.

Measurement of Strange Quark Contributions to the Nucleon's Form Factors at Q^2=0.230 (GeV/c)^2

We report on a measurement of the parity-violating asymmetry in the scattering of longitudinally polarized electrons on unpolarized protons at a $Q^2$ of 0.230 (GeV/c)^2 and a scattering angle of \theta_e = 30^o - 40^o. Using a large acceptance fast PbF_2 calorimeter with a solid angle of \Delta\Omega = 0.62 sr the A4 experiment is the first parity violation experiment to count individual scattering events. The measured asymmetry is A_{phys} =(-5.44 +- 0.54_{stat} +- 0.27_{\rm sys}) 10^{-6}. The Standard Model expectation assuming no strangeness contributions to the vector form factors is $A_0=(-6.30 +- 0.43) 10^{-6}$. The difference is a direct measurement of the strangeness contribution to the vector form factors of the proton. The extracted value is G^s_E + 0.225 G^s_M = 0.039 +- 0.034 or F^s_1 + 0.130 F^s_2 = 0.032 +- 0.028.Comment: 5 pages, 3 figures, submitted to Phys. Rev. Letters on Dec 11, 200