248 research outputs found
Full phase stabilization of a Yb:fiber femtosecond frequency comb via high-bandwidth transducers
We present full phase stabilization of an amplified Yb:fiber femtosecond
frequency comb using an intra-cavity electro-optic modulator and an
acousto-optic modulator. These transducers provide high servo bandwidths of 580
kHz and 250 kHz for frep and fceo, producing a robust and low phase noise fiber
frequency comb. The comb was self-referenced with an f - 2f interferometer and
phase locked to an ultra-stable optical reference used for the JILA Sr optical
clock at 698 nm, exhibiting 0.21 rad and 0.47 rad of integrated phase errors
(over 1 mHz - 1 MHz) respectively. Alternatively, the comb was locked to two
optical references at 698 nm and 1064 nm, obtaining 0.43 rad and 0.14 rad of
integrated phase errors respectively
Phase-stabilized, 1.5-W frequency comb at 2.8 to 4.8 micron
We present a high-power optical parametric oscillator-based frequency comb in
the mid-infrared wavelength region using periodically poled lithium niobate.
The system is synchronously pumped by a 10-W femtosecond Yb:fiber laser
centered at 1.07 um and is singly resonant for the signal. The idler (signal)
wavelength can be continuously tuned from 2.8 to 4.8 um (1.76 to 1.37 um) with
a simultaneous bandwidth as high as 0.3 um and a maximum average idler output
power of 1.50 W. We also demonstrate the performance of the stabilized comb by
recording the heterodyne beat with a narrow-linewidth diode laser. This OPO is
an ideal source for frequency comb spectroscopy in the mid-IR.Comment: 4 figure
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Low noise electro-optic comb generation by fully stabilizing to a mode-locked fiber comb.
A fully stabilized EO comb is demonstrated by phase locking the two degrees of freedom of an EO comb to a low noise mode-locked fiber comb. Division/magnification of residual phase noise of locked beats is observed by measuring an out-of-loop beat. By phase locking the 200 th harmonics of the EO comb and a driving cw frequency to a fiber comb, a record low phase noise EO comb across +/- 200 harmonics (from 1544.8 nm to 1577.3 nm) is demonstrated
Prospects for precision measurements of atomic helium using direct frequency comb spectroscopy
We analyze several possibilities for precisely measuring electronic
transitions in atomic helium by the direct use of phase-stabilized femtosecond
frequency combs. Because the comb is self-calibrating and can be shifted into
the ultraviolet spectral region via harmonic generation, it offers the prospect
of greatly improved accuracy for UV and far-UV transitions. To take advantage
of this accuracy an ultracold helium sample is needed. For measurements of the
triplet spectrum a magneto-optical trap (MOT) can be used to cool and trap
metastable 2^3S state atoms. We analyze schemes for measuring the two-photon
interval, and for resonant two-photon excitation to high
Rydberg states, . We also analyze experiments on the
singlet-state spectrum. To accomplish this we propose schemes for producing and
trapping ultracold helium in the 1^1S or 2^1S state via intercombination
transitions. A particularly intriguing scenario is the possibility of measuring
the transition with extremely high accuracy by use of
two-photon excitation in a magic wavelength trap that operates identically for
both states. We predict a ``triple magic wavelength'' at 412 nm that could
facilitate numerous experiments on trapped helium atoms, because here the
polarizabilities of the 1^1S, 2^1S and 2^3S states are all similar, small, and
positive.Comment: Shortened slightly and reformatted for Eur. Phys. J.
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All polarization-maintaining Er fiber-based optical frequency combs with nonlinear amplifying loop mirror.
A fully stabilized all polarization-maintaining Er frequency comb with a nonlinear amplifying loop mirror with below 0.2 rad carrier-envelope-offset frequency phase noise is demonstrated. The integrated timing jitter is measured as 40 attosecond from 10 kHz to 10 MHz, which is the lowest value of any Er fiber frequency comb to date
Delivery of BMP-2 by two clinically available apatite materials: In vitro and in vivo comparison
Bone morphogenetic proteins (BMPs) are deposited in bone and responsible for osteoinduction. The interplay between delivery system and BMP, resulting in a characteristic release profile, is crucial for clinical success. We here report on two apatite based commercially available granules which could potentially be used in a combination product with recombinant human BMP-2 (rhBMP-2). Regardless of their similar chemistry, their interaction with rhBMP-2 differs. Deproteinized bovine bone matrix (DBBM), a clinically well-established bone substitute, has a high affinity to rhBMP-2 and releases only 50% of the growth factor during the first 2 weeks in vitro. Activity of the physio-adsorbed rhBMP-2 is indicated by an enhanced bone augmentation in vivo. In contrast, all rhBMP-2 delivered in combination with synthetic hydroxyapatite/β-tricalcium phosphate (HA/TCP) granules is released during the first 24 h. For both HA/TCP and DBBM, the released rhBMP-2 is active in vitro. Our results suggest that the different release behavior from these two apatite granules is due to the 1000-fold higher specific surface area of DBBM compared to HA/TCP
Multimodal imaging of pancreatic beta cells in vivo by targeting transmembrane protein 27 (TMEM27)
Aims/hypothesis: Non-invasive diagnostic tools specific for pancreatic beta cells will have a profound impact on our understanding of the pathophysiology of metabolic diseases such as diabetes. The objective of this study was to use molecular imaging probes specifically targeting beta cells on human samples and animal models using state-of-the-art imaging modalities (fluorescence and PET) with preclinical and clinical perspective. Methods: We generated a monoclonal antibody, 8/9-mAb, targeting transmembrane protein 27 (TMEM27; a surface N-glycoprotein that is highly expressed on beta cells), compared its expression in human and mouse pancreas, and demonstrated beta cell-specific binding in both. In vivo imaging was performed in mice with subcutaneous insulinomas overexpressing the human TMEM27 gene, or transgenic mice with beta cell-specific hTMEM27 expression under the control of rat insulin promoter (RIP-hTMEM27-tg), using fluorescence and radioactively labelled antibody, followed by tissue ex vivo analysis and fluorescence microscopy. Results: Fluorescently labelled 8/9-mAb showed beta cell-specific staining on human and mouse pancreatic sections. Real-time PCR on islet cDNA indicated about tenfold higher expression of hTMEM27 in RIP-hTMEM27-tg mice than in humans. In vivo fluorescence and PET imaging in nude mice with insulinoma xenografts expressing hTMEM27 showed high 8/9-mAb uptake in tumours after 72h. Antibody homing was also observed in beta cells of RIP-hTMEM27-tg mice by in vivo fluorescence imaging. Ex vivo analysis of intact pancreas and fluorescence microscopy in beta cells confirmed these findings. Conclusions/interpretation: hTMEM27 constitutes an attractive target for in vivo visualisation of pancreatic beta cells. Studies in mouse insulinoma models and mice expressing hTMEM27 demonstrate the feasibility of beta cell-targeted in vivo imaging, which is attractive for preclinical investigations and holds potential in clinical diagnostic
220 fs Er-Yb:glass laser mode-locked by a broadband low-loss Si/Ge saturable absorber
We demonstrate femtosecond performance of an ultra-broadband
high-index-contrast saturable Bragg reflector consisting of a
silicon/silicon-dioxide/germanium structure that is fully compatible with CMOS
processing. This device offers a reflectivity bandwidth of over 700 nm and
sub-picosecond recovery time of the saturable loss. It is used to achieve
mode-locking of an Er-Yb:glass laser centered at 1540 nm, generating 220 fs
pulses, with the broadest output spectrum to date
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