112 research outputs found

    Low-cost image annotation for supervised machine learning. Application to the detection of weeds in dense culture

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    An open problem in robotized agriculture is to detect weeds in dense culture. This problem can be addressed with computer vision and machine learning. The bottleneck of supervised approaches lay in the manual annotation of training images. We propose two different approaches for detecting weeds position to speed up this process. The first approach is using synthetic images and eye-tracking to annotated images [4] which is at least 30 times faster than manual annotation by an expert, the second approach is based on real RGB and depth images collected via Kinect v2 sensor. We generated a data set of 150 synthetic images which weeds were randomly positioned on it. Images were gazed by two observers. Eye tracker sampled eye position during the execution of this task [5, 6]. Area of interest was recorded as rectangular patches. A patch is considered as including weeds if the average fixation time in this patch exceeds 1.04 seconds. The quality of visual annotation by eye-tracking is assessed by two ways. First, direct comparison of visual annotation with ground-truth which is shown an average 94.7% of all fixations on an image which fell within ground-truth bounding-boxes. Second, as shown in fig.1 eye-tracked annotated data is used as a training data set in four machine learning approaches and compare the recognition rate with the ground-truth. These four machine learning methods are tested in order to assess the quality of the visual annotation. These methods correspond to handcrafted features adapted to texture characterization. They are followed by a linear support vector machine binary classifier. The table 1 gives the average accuracy and standard deviation. Experimental results prove that visual eye-tracked annotated data are almost the same as in-silico ground-truth and performances of supervised machine learning on eye-tracked annotated data are very close to the one obtained with ground-truth

    The Nab Experiment: A Precision Measurement of Unpolarized Neutron Beta Decay

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    Neutron beta decay is one of the most fundamental processes in nuclear physics and provides sensitive means to uncover the details of the weak interaction. Neutron beta decay can evaluate the ratio of axial-vector to vector coupling constants in the standard model, λ=gA/gV\lambda = g_A / g_V, through multiple decay correlations. The Nab experiment will carry out measurements of the electron-neutrino correlation parameter aa with a precision of ÎŽa/a=10−3\delta a / a = 10^{-3} and the Fierz interference term bb to ÎŽb=3×10−3\delta b = 3\times10^{-3} in unpolarized free neutron beta decay. These results, along with a more precise measurement of the neutron lifetime, aim to deliver an independent determination of the ratio λ\lambda with a precision of Ύλ/λ=0.03%\delta \lambda / \lambda = 0.03\% that will allow an evaluation of VudV_{ud} and sensitively test CKM unitarity, independent of nuclear models. Nab utilizes a novel, long asymmetric spectrometer that guides the decay electron and proton to two large area silicon detectors in order to precisely determine the electron energy and an estimation of the proton momentum from the proton time of flight. The Nab spectrometer is being commissioned at the Fundamental Neutron Physics Beamline at the Spallation Neutron Source at Oak Ridge National Lab. We present an overview of the Nab experiment and recent updates on the spectrometer, analysis, and systematic effects.Comment: Presented at PPNS201

    Echocardiographic evaluation of mitral geometry in functional mitral regurgitation

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    <p>Abstract</p> <p>Objectives</p> <p>We sought to evaluate the geometric changes of the mitral leaflets, local and global LV remodeling in patients with left ventricular dysfunction and varying degrees of Functional mitral regurgitation (FMR).</p> <p>Background</p> <p>Functional mitral regurgitation (FMR) occurs as a consequence of systolic left ventricular (LV) dysfunction caused by ischemic or nonischemic cardiomyopathy. Mitral valve repair in ischemic MR is one of the most controversial topic in surgery and proper repairing requires an understanding of its mechanisms, as the exact mechanism of FMR are not well defined.</p> <p>Methods</p> <p>136 consecutive patients mean age of 55 with systolic LV dysfunction and FMR underwent complete echocardiography and after assessing MR severity, LV volumes, Ejection Fraction, LV sphericity index, C-Septal distance, Mitral valve annulus, Interpapillary distance, Tenting distance and Tenting area were obtained.</p> <p>Results</p> <p>There was significant association between MR severity and echocardiogarphic indices (all p values < 0.001). Severe MR occurred more frequently in dilated cardiomyopathy (DCM) patients compared to ischemic patients, (p < 0.001). Based on the model, only Mitral valve tenting distance (TnD) (OR = 22.11, CI 95%: 14.18 – 36.86, p < 0.001) and Interpapillary muscle distance (IPMD), (OR = 6.53, CI 95%: 2.10 – 10.23, p = 0.001) had significant associations with MR severity.</p> <p>Mitral annular dimensions and area, C-septal distance and sphericity index, although greater in patients with severe regurgitation, did not significantly contribute to FMR severity.</p> <p>Conclusion</p> <p>Degree of LV enlargement and dysfunction were not primary determinants of FMR severity, therefore local LV remodeling and mitral valve apparatus deformation are the strongest predictors of functional MR severity.</p

    Pre-formulation and systematic evaluation of amino acid assisted permeability of insulin across in vitro buccal cell layers

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    The aim of this work was to investigate alternative safe and effective permeation enhancers for buccal peptide delivery. Basic amino acids improved insulin solubility in water while 200 and 400 ”g/mL lysine significantly increased insulin solubility in HBSS. Permeability data showed a significant improvement in insulin permeation especially for 10 ”g/mL of lysine (p < 0.05) and 10 ”g/mL histidine (p < 0.001), 100 ”g/mL of glutamic acid (p < 0.05) and 200 ”g/mL of glutamic acid and aspartic acid (p < 0.001) without affecting cell integrity; in contrast to sodium deoxycholate which enhanced insulin permeability but was toxic to the cells. It was hypothesized that both amino acids and insulin were ionised at buccal cavity pH and able to form stable ion pairs which penetrated the cells as one entity; while possibly triggering amino acid nutrient transporters on cell surfaces. Evidence of these transport mechanisms was seen with reduction of insulin transport at suboptimal temperatures as well as with basal-to-apical vectoral transport, and confocal imaging of transcellular insulin transport. These results obtained for insulin is the first indication of a possible amino acid mediated transport of insulin via formation of insulin-amino acid neutral complexes by the ion pairing mechanism

    Characterisation of sugar beet (Beta vulgaris L. ssp. vulgaris) varieties using microsatellite markers

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    <p>Abstract</p> <p>Background</p> <p>Sugar beet is an obligate outcrossing species. Varieties consist of mixtures of plants from various parental combinations. As the number of informative morphological characteristics is limited, this leads to some problems in variety registration research.</p> <p>Results</p> <p>We have developed 25 new microsatellite markers for sugar beet. A selection of 12 markers with high quality patterns was used to characterise 40 diploid and triploid varieties. For each variety 30 individual plants were genotyped. The markers amplified 3-21 different alleles. Varieties had up to 7 different alleles at one marker locus. All varieties could be distinguished. For the diploid varieties, the expected heterozygosity ranged from 0.458 to 0.744. The average inbreeding coefficient F<sub>is </sub>was 0.282 ± 0.124, but it varied widely among marker loci, from F<sub>is </sub>= +0.876 (heterozygote deficiency) to F<sub>is </sub>= -0.350 (excess of heterozygotes). The genetic differentiation among diploid varieties was relatively constant among markers (F<sub>st </sub>= 0.232 ± 0.027). Among triploid varieties the genetic differentiation was much lower (F<sub>st </sub>= 0.100 ± 0.010). The overall genetic differentiation between diploid and triploid varieties was F<sub>st </sub>= 0.133 across all loci. Part of this differentiation may coincide with the differentiation among breeders' gene pools, which was F<sub>st </sub>= 0.063.</p> <p>Conclusions</p> <p>Based on a combination of scores for individual plants all varieties can be distinguished using the 12 markers developed here. The markers may also be used for mapping and in molecular breeding. In addition, they may be employed in studying gene flow from crop to wild populations.</p

    Antimicrobial activity of ProRoot MTA in contact with blood

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    Dental materials based on Portland cement, which is used in the construction industry have gained popularity for clinical use due to their hydraulic properties, the interaction with tooth tissue and their antimicrobial properties. The antimicrobial properties are optimal in vitro. However in clinical use contact with blood may affect the antimicrobial properties. This study aims to assess whether antimicrobial properties of the Portland cement-based dental cements such as mineral trioxide aggregate (MTA) are also affected by contact with blood present in clinical situations. ProRoot MTA, a Portland cement-based dental cement was characterized following contact with water, or heparinized blood after 1 day and 7 days aging. The antimicrobial activity under the mentioned conditions was assessed using 3 antimicrobial tests: agar diffusion test, direct contact test and intratubular infection test. MTA in contact with blood was severely discoloured, exhibited an additional phosphorus peak in elemental analysis, no calcium hydroxide peaks and no areas of bacterial inhibition growth in the agar diffusion test were demonstrated. ProRoot MTA showed limited antimicrobial activity, in both the direct contact test and intratubular infection test. When aged in water ProRoot MTA showed higher antimicrobial activity than when aged in blood. Antimicrobial activity reduced significantly after 7 days. Further assessment is required to investigate behaviour in clinical situations.ERDF (Malta) for the financing of the testing equipment through the project: “Developing an Interdisciplinary Material Testing and Rapid Prototyping R&D Facility” (Ref. no. 012)
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