Role of Growth Factors in the Adhesive Process in the Abdominal Cavity

Fibroproliferative diseases have been described in the lungs, kidneys, liver, eyes, heart, skin, and abdomen. Each fibrous pathology has causal factors and pathological manifestations characteristic of this organ and this condition. However, there are common mechanisms underlying many fibrous pathologies. This gives potential value to studies focused on specific processes, among which is the adhesive process in the abdominal cavity. The study of growth factors in the formation of connective tissue contributes to a better understanding of the pathogenetic picture in this pathology. It is shown that vascular endothelial growth factor, fibroblast growth factor, transforming growth factor, platelet growth factor, insulin-like growth factor and keratinocyte growth factor participate in the development of abdominal adhesions. Each of these factors contributes significantly to the formation of peritoneal fibrosis.Thus, damage to any tissue initiates a complex multistage process, which is regulated by a large number of cytokines and growth factors. Growth factors control cell migration, proliferation, differentiation, and survival. In addition, they are able to influence the expression of other factors involved in the regenerative response. Understanding the process that develops during the formation of the adhesive process in the abdominal cavity and the growth factors affecting it is important for their further use in order to prevent the pathological process

Ways of increase of competitiveness of the enterprise

На сегодняшний день важным термином в экономике является термин «конкурентоспособность».To date, an important term in Economics is the term "competitiveness". The main condition for introduction of the country into the global economy is improving the competitiveness of firms in a market and the national economy

Assessment of Potential Cytotoxicity During Vital Observation at the BioStation CT

In recent decades, the methods based on the cell technologies have become more relevant for medical and pharmaceutical research. In this paper, weconsidered the BioStation CT using for continuous in vivo observation of Ehrlich ascitic carcinoma cell culture under the influence of two active substances in different concentrations (from 1.25 to 20 mg/l). As a result of the carcinoma cells condition monitoring for 4.5 days it was shown that substance No 2 does not affect the cell viability while substance No 1 causes the carcinoma cell death, and this active substance effect is dose-dependent. Photodocumentation at two-hour intervals allowed us to research differences in the rate of cell destruction (intensive disintegration in the first day with further stabilization of the living cells number or gradual cell death throughout the experiment), as well as differences in the time of 50 % mortality reaching. Thus in the study it was demonstrated that due to the fact that the BioStation CT combines the properties of a CO2 -incubator and a microscope this device is promising for toxicological studies and significantly expands the detection possibilities of processes occurring with living cells for a sufficiently long time period making possible further analysis of the cell behavior characteristics throughout the experiment, and that is fundamentally different from the systems allowing only the final result fixation of long-term active substance exposure

The Use of Drainage Structures in Abdominal Surgery in the Postoperative Period (Experimental Study)

Preventive drainage of the abdominal cavity after gastrointestinal surgery is widely used. However, debates about the need for abdominal drainage have not subsided so far.The aim of the study: to establish the effect of the duration of the use of drains on the development of postoperative complications in the experiment.Materials and methods. For the experiments, male Wistar rats weighing 220-250 g at the age of 9 months were used. A mid laparotomy was performed, then the serous-muscular layer of the cecum was opened, 1 cm long, followed by suturing the wound with a self-twisting suture, and the parietal peritoneum of the right lateral canal was sized 1.5х1.5 cm. A silicone catheter was installed. The study was conducted in two groups. In the first group, the drainage was removed after 24 hours, in the second - after three days. The study was conducted on the 3rd, 7th, 14th and 30th day, 6 animals in each group/at each term. The severity of the adhesive process in the abdominal cavity, the presence of an inflammatory process in the peritoneum, and hematological parameters were evaluated.Results. It was established that the severity of the adhesion process in groups in all periods of observation was comparable. The severity of purulent-inflammatory processes in the abdominal cavity on the 14th and 30th day was significantly lower when installing drainage for 1 day than 3 days.Conclusion. A study with the installation of drainage in the absence of infection of the peritoneum showed that the duration of the installation of the drain adversely affects the incidence of purulent-inflammatory changes in the abdominal cavity, which must be taken into account when planning the use of drainages in abdominal surgery

FUNCTIONAL ANALYSIS AND FUNCTIONAL MAPS OF QUALIFICATIONS IN ECVET CONTEXT

Introduction. Activation of the process of internationalisation and mobility in Russian Vocational Education and Training (VET) is quite possible by adapting principles and methodology of the European Credit Transfer System for Vocational Education and Training (ECVET). The need to achieve transparency of qualifications, recognition of learning outcomes in view of achieving qualifications, accumulation, comparison and transfer of learning outcomes becomes more apparent and urgent for integration processes of VET systems in Russia and European Union countries and is currently under consideration by the RUECVET project team. The aimis to adjust application of functional analysis to description of an occupation in terms of work functions with the following conversion of the defined functions into sets of learning outcomes necessary for characterizing a qualification. The ECVET principles and technical specifications form the context of the research in order to develop an adequate VET study programme for achieving the correspondent qualification of level 4 or 5 of the European Qualifications Framework (EQF) in the Russian education environment. Methodology and research methods. The methodology is based on the functional analysis of an occupational sector and the functions performed within it in such a manner that the following identification of the correspondent units of learning outcomes for the relevant qualification can be fulfilled. The research has been conducted by means of the content-analysis, modelling and expert estimation method.Results and scientific novelty. Comparative analysis of approaches to the design of occupations and qualifications in both national and European environments is presented in the article. The functional analysis is used for allocation of work functions and creation of units of learning outcomes related to a particular qualification. The procedure of applying functional analysis to an occupation as well as the technology of consequent construction of the functional map for the correspondent qualification taking into account the relevant educational and occupational standards and sectoral preferences have been elaborated. The result of the study is adaptation of methodology of functional analysis and verification of the developed procedure when constructing a functional map of the chosen qualification. The procedures are illustrated on the qualification of a lawyer achieved in the VET study programme “Legislation and Social Protection”, equivalent to level 5 of the European Qualifications Framework (EQF). Successful experiments with occupations of welders, electricians, web designers, hospitality managers and others related to the qualifications of level 4 and 5 of EQF have been also completed in RUECVET project, although are not described in the article due to the restricted volume.Practical significance. The derived procedures are useful for development of a set of detailed learning outcomes from functional mapping by allocating knowledge and skills and using the concept of unit of learning outcomes for compilation of a qualification in accordance with the relevant educational and occupational standards and employers’ understanding how the real work should be performed. The procedures are useful also for identifying units of learning outcomes to build an adequate VET study programme in ECVET context of Russian educational system. Введение. Активизации процессов мобильности и интернационализации в системе среднего специального и высшего российского образования способствует внедрение в нее методологии и инструментария European Credit System for Vocational Education and Training – ECVET (Европейской системы зачетных единиц для профессионального образования и обучения). Необходимость прозрачности приобретаемых студентами квалификаций, устанавливающихся посредством единых механизмов оценки, признания, накопления и сравнения результатов профессиональной подготовки, становится более очевидной и актуальной в связи с интеграцией образовательного пространства России и Европы. В настоящее время изыскания в этом направлении ведутся группой ученых, участвующих в пилотном международном проекте RUECVET1. Цель публикации – представить разработанную авторским коллективом исследователей методику описания квалификации – определения подразумевающихся под ней трудовых функций с последующим их преобразованием в показатели результатов обучения, характеризующих данную квалификацию, которая присваивается по завершении программы профессиональной подготовки. Методология и методы. В ходе работы в качестве методолого-методической базы использовались функциональный и сравнительный виды анализа, методы контент-анализа, моделирования и экспертной оценки. Результаты и научная новизна. Сопоставлены подходы к проектированию профессий и квалификаций, применяющиеся в странах Евросоюза и в Российской Федерации. Исходя из синтезированного опыта разных государств, адаптированного к российским экономическим и образовательным реалиям, выделены обобщенные трудовые функции ряда специальностей, соответствующих 4-му или 5-му уровням Европейской рамки квалификаций (EQF). На основе этих функций и с учетом содержания действующих образовательных и профессиональных стандартов, а также мнения работодателей сформированы блоки результатов обучения, требующихся для достижения определенного квалификационного уровня. Продемонстрирован процесс создания функциональной карты конкретной квалификации. Технология построения функциональной карты проиллюстрирована на примере специальности среднего профессионального образования «Право и организация социального обеспечения» (в EQF относится к 5-му уровню). Отмечается, что ряд подобных успешных экспериментов был проведен с профессиями сварщика, электрика, веб-дизайнера, специалиста по гостеприимству и др. Практическое значение. Предлагаемые процедуры детализации профессиональных знаний, умений, навыков и компетенций и комплектования из них блоков результатов обучения в виде функциональных карт для конструирования и оценки квалификации позволяют разрабатывать адекватные требованиям мирового рынка труда программы профессионального образования. Технология, основанная на функциональном анализе, отвечает принципам и техническим требованиям ECVET и дает возможность измерять и регистрировать результаты обучения согласно международной системе зачетных единиц (кредитов).Education, Audio-visual and Culture Executive Agency, Erasmus+, ref. № 574097-EPP-1–2016–1-CYEPPKA2-CBHE-JPИсполнительное агентство по образованию, аудиовизуальным средствам и культуре (проект № 574097-EPP-1–2016–1-CYEPPKA2-CBHE-J

The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages

MPO (myeloperoxidase) catalyses the oxidation of chloride, bromide and thiocyanate by hydrogen peroxide to HOCl (hypochlorous acid), HOBr (hypobromous acid) and HOSCN (hypothiocyanous acid) respectively. Specificity constants indicate that SCN− is a major substrate for MPO. HOSCN is also a major oxidant generated by other peroxidases including salivary, gastric and eosinophil peroxidases. While HOCl and HOBr are powerful oxidizing agents, HOSCN is a less reactive, but more specific, oxidant which targets thiols and especially low pKa species. In the present study we show that HOSCN targets cysteine residues present in PTPs (protein tyrosine phosphatases) with this resulting in a loss of PTP activity for the isolated enzyme, in cell lysates and intact J774A.1 macrophage-like cells. Inhibition also occurs with MPO-generated HOCl and HOBr, but is more marked with MPO-generated HOSCN, particularly at longer incubation times. This inhibition is reversed by dithiothreitol, particularly at early time points, consistent with the reversible oxidation of the active site cysteine residue to give either a cysteine–SCN adduct or a sulfenic acid. Inhibition of PTP activity is associated with increased phosphorylation of p38a and ERK2 (extracellular-signal-regulated kinase 2) as detected by Western blot analysis and phosphoprotein arrays, and results in altered MAPK (mitogen-activated protein kinase) signalling. These data indicate that the highly selective targeting of some protein thiols by HOSCN can result in perturbation of cellular phosphorylation and altered cell signalling. These changes occur with (patho)physiological concentrations of SCN− ions, and implicate HOSCN as an important mediator of inflammation-induced oxidative damage, particularly in smokers who have elevated plasma levels of SCN−

Pycnogenol® and Ginkgo biloba extract: effect on peroxynitrite-oxidized sarcoplasmic reticulum Ca2+-ATPase

The effect of two natural standardized plant extracts, Pycnogenol® and EGb 761, on sarcoplasmic reticulum Ca2+-ATPase (SERCA) activity and posttranslational modifications induced by peroxynitrite was investigated to assess their possible protective role. EGb 761 was found to have a protective effect on SERCA activity in the concentration range of 5–40 µg/ml. On the other hand, Pycnogenol® caused a decrease of SERCA activity at concentrations of 25 µg/ml. EGb 761 did not prevent protein carbonyl formation upon oxidation with peroxynitrite. On the contrary, Pycnogenol® at the concentrations of 5 and 10 µg/ml significantly decreased the level of protein carbonyls by 44% and 54%, respectively. Neither Pycnogenol® nor EGb 761 exerted a protective effect against thiol group oxidation.The plant extracts studied modulated peroxynitrite-injured SERCA activity by different ways and failed to correlate with posttranslational modifications. Their effect seems to be associated with their ability to change SERCA conformation rather than by their antioxidant capacity

Flavonoids in prevention of diseases with respect to modulation of Ca-pump function

Flavonoids, natural phenolic compounds, are known as agents with strong antioxidant properties. In many diseases associated with oxidative/nitrosative stress and aging they provide multiple biological health benefits. Ca2+-ATPases belong to the main calcium regulating proteins involved in the balance of calcium homeostasis, which is impaired in oxidative/nitrosative stress and related diseases or aging. The mechanisms of Ca2+-ATPases dysfunction are discussed, focusing on cystein oxidation and tyrosine nitration. Flavonoids act not only as antioxidants but are also able to bind directly to Ca2+-ATPases, thus changing their conformation, which results in modulation of enzyme activity

ICE CREAM WITH A COMBINED COMPOSITION OF RAW MATERIALS

Dairy products are the product of daily demand. Nowadays actively assimilate new types of raw materials, tech-nology, formulation. One of the propagation methods of enriching dairy products is a combination of milk and vegetable raw materials. The possibility of making a concentrated paste of Jerusalem artichoke in dairy products was investigated. The ice cream sundae "Vanilla" was chosen as the object of research

FUNCTIONAL ANALYSIS AND FUNCTIONAL MAPS OF QUALIFICATIONS IN ECVET CONTEXT

Introduction. Activation of the process of internationalisation and mobility in Russian Vocational Education and Training (VET) is quite possible by adapting principles and methodology of the European Credit Transfer System for Vocational Education and Training (ECVET). The need to achieve transparency of qualifications, recognition of learning outcomes in view of achieving qualifications, accumulation, comparison and transfer of learning outcomes becomes more apparent and urgent for integration processes of VET systems in Russia and European Union countries and is currently under consideration by the RUECVET project team.The aimis to adjust application of functional analysis to description of an occupation in terms of work functions with the following conversion of the defined functions into sets of learning outcomes necessary for characterizing a qualification. The ECVET principles and technical specifications form the context of the research in order to develop an adequate VET study programme for achieving the correspondent qualification of level 4 or 5 of the European Qualifications Framework (EQF) in the Russian education environment. Methodology and research methods. The methodology is based on the functional analysis of an occupational sector and the functions performed within it in such a manner that the following identification of the correspondent units of learning outcomes for the relevant qualification can be fulfilled. The research has been conducted by means of the content-analysis, modelling and expert estimation method.Results and scientific novelty. Comparative analysis of approaches to the design of occupations and qualifications in both national and European environments is presented in the article. The functional analysis is used for allocation of work functions and creation of units of learning outcomes related to a particular qualification. The procedure of applying functional analysis to an occupation as well as the technology of consequent construction of the functional map for the correspondent qualification taking into account the relevant educational and occupational standards and sectoral preferences have been elaborated. The result of the study is adaptation of methodology of functional analysis and verification of the developed procedure when constructing a functional map of the chosen qualification. The procedures are illustrated on the qualification of a lawyer achieved in the VET study programme “Legislation and Social Protection”, equivalent to level 5 of the European Qualifications Framework (EQF). Successful experiments with occupations of welders, electricians, web designers, hospitality managers and others related to the qualifications of level 4 and 5 of EQF have been also completed in RUECVET project, although are not described in the article due to the restricted volume.Practical significance. The derived procedures are useful for development of a set of detailed learning outcomes from functional mapping by allocating knowledge and skills and using the concept of unit of learning outcomes for compilation of a qualification in accordance with the relevant educational and occupational standards and employers’ understanding how the real work should be performed. The procedures are useful also for identifying units of learning outcomes to build an adequate VET study programme in ECVET context of Russian educational system