Comparative Advantage and Labor Protections in Free Trade Agreements: Making Labor Protections in Trade Agreements Practical and Effective

The tension between competitiveness in international trade and the improvement of living standards has become a central controversy in negotiating trade agreements. Under pressure from the labor rights movement over the course of the last twenty-five years, the United States has regularly advocated for the inclusion of labor standards in trade relationships. Generally, governments in developing countries resist the incorporation of labor protections in trade agreements because of a belief that labor protections diminish a nation’s competitiveness in the international marketplace. Labor rights advocates, particularly in the United States, have fought for the inclusion of labor rights in trade agreements as a means of lifting living standards in developing countries, and preserving them in developed countries, to avoid and international “race to the bottom” where countries try to out-compete each other by keeping labor costs as low as possible. This article argues that trade agreements should include labor provisions that provide effective protection for core labor rights. Such protection should ensure that countries have domestic laws protecting core labor rights and that those countries enforce those laws. Further, the labor standards provisions must include (1) a compelling incentive program to reward developing nations that improve labor conditions and comply with labor standards and (2) an effective enforcement mechanism to penalize countries that violate the labor provisions. Section one of this article provides a short summary of the history of labor standards in United States trade policy and some of the debates surrounding the movement to include labor protections in the North American Free Trade Agreement (NAFTA) and the Central American-Dominican Republic Free Trade Agreement (CAFTA-DR, referred to in this article simply as “CAFTA”). Section two provides an overview of traditional economic theory supporting free trade and several arguments that call that theory into question. Finally, section three offers a defense regulating trade and makes specific policy recommendations regarding the inclusion of labor standards provisions in future trade agreements

Card Check Recognition: the Ongoing Legal and Legislative Battle

A great debate has been brewing for years over whether unions should be able to organize employees outside of the traditional election procedures provided by the National Labor Relations Act (“NLRA” or “the Act”). Typically, in an organizing drive, a union solicits support from employees to indicate a desire to run a National Labor Relations Board (“NLRB” or “Board”) election. The union does this by collecting cards from employees affirming the employees’ desire to have a representation election. If the union collects valid cards from at least one-third of eligible employees in the appropriate bargaining unit, the union may then petition for a Board election. If the majority of employees support the union in the election, then the employer must recognize the union and bargain in good faith with the union for an initial labor contract. During the period between the representation election and the completion of the first collective bargaining agreement, the NLRA bars the employer, the employees, and competing labor unions from challenging the representative union’s majority status for a reasonable period of time. Board elections have long been the preferred method of obtaining union recognition. Recently, however, many unions have begun focusing on another organizing strategy known as card check recognition. In card check recognition campaigns, a union demonstrates majority support of the employees in a bargaining unit by collecting cards from a majority of employees that express the employees’ desire to have the union represent them and gaining the consent of the employer to recognize the union as the representative of the employees without the formality of a Board election. Unions generally negotiate neutrality agreements with employers prior to launching a card check campaign to ensure the employer will not oppose the union during the organizing drive. If the employer refuses to recognize the union, then the union may petition the Board for an election. Though the NLRB and courts prefer Board elections, unions generally prefer card check campaigns because they are vastly more likely to result in a successful unionization drive than Board elections. However, anti-union groups criticize this approach as an unfair coercion of employees to join unions. In 2004, the NLRB granted review of several cases that called into question the underlying principles of card check recognition. In Dana Corporation and Metaldyne, the Board, reversing a Regional Director’s dismissal of the complaints, decided to hear cases where the dispute concerned whether or not voluntary employer recognition of a union based on a card check campaign should be given “election bar quality.” An election bar is the period of time during which a union’s right to represent the bargaining unit cannot be challenged by an employer, employees, or another union. The Board took the cases to determine whether unions certified as a result of a card check campaign should be granted the same amount of protection as unions certified through a Board election. In Shaw’s Supermarkets, the Board reversed the Regional Director’s dismissal of a case where employees’ attempted to decertify the union, which the employer voluntarily recognized based on the union’s presentation of signed cards supporting unionization. Again, the Board’s decision to hear such a case indicated its willingness to consider treating union recognition based on card check as inferior to recognition based on Board elections. While awaiting the Board’s verdict, a rich legislative debate over card check recognition has been renewed. This year, Republicans and Democrats introduced opposing legislation specifically related to card check recognition. Republicans introduced the Secret Ballot Protection Act (“SBPA”), which would make NLRB elections the exclusive method for union recognition, prohibiting employers from voluntarily recognizing a union based on a demonstration of majority support. Across the aisle, Democrats introduced the Employee Free Choice Act (“EFCA”), which would mandate that an employer recognize a union upon demonstration of majority support by submission of employee-signed union authorization cards to the NLRB. This article argues: (1) that the NLRB should define the “reasonable period of time” for a certification bar following union recognition based on a card check campaign to be commensurate with election bar quality; (2) that the NLRB should not narrow the availability of card check recognition as an organizing tool in its resolution of Shaw’s Supermarkets; (3) that Congress should reject the Secret Ballot Protection Act’s effort to prohibit card check recognition campaigns; and finally, (4) that Congress should, perhaps with some minor amendments, pass the Employee Free Choice Act. Section II of this article provides contextual background surrounding the issue of card check recognition. Section III provides legal analysis of Dana Corporation and Shaw’s Supermarkets, including legal history supporting card-check recognition as a legitimate organizing tool. Section IV analyzes the legislative efforts to prohibit and to codify card check recognition

The Transcriptome of SH-SY5Y at Single-Cell Resolution: A CITE-Seq Data Analysis Workflow

Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) is a recently established multimodal single cell analysis technique combining the immunophenotyping capabilities of antibody labeling and cell sorting with the resolution of single-cell RNA sequencing (scRNA-seq). By simply adding a 12-bp nucleotide barcode to antibodies (cell hashing), CITE-seq can be used to sequence antibody-bound tags alongside the cellular mRNA, thus reducing costs of scRNA-seq by performing it at the same time on multiple barcoded samples in a single run. Here, we illustrate an ideal CITE-seq data analysis workflow by characterizing the transcriptome of SH-SY5Y neuroblastoma cell line, a widely used model to study neuronal function and differentiation. We obtained transcriptomes from a total of 2879 single cells, measuring an average of 1600 genes/cell. Along with standard scRNA-seq data handling procedures, such as quality checks and cell filtering procedures, we performed exploratory analyses to identify most stable genes to be possibly used as reference housekeeping genes in qPCR experiments. We also illustrate how to use some popular R packages to investigate cell heterogeneity in scRNA-seq data, namely Seurat, Monocle, and slalom. Both the CITE-seq dataset and the code used to analyze it are freely shared and fully reusable for future research

Multimodality imaging of chronic tophaceous gout

The diagnosis of gout is usually based on clinical presentation and laboratory findings. Imaging plays a role in the assessment and grading of articular damage related to chronic, long-standing disease, which is characterized by granulomatous synovitis, tophi, and erosions. Multimodality imaging of chronic tophaceous gout may be useful in clinical practice for a variety of purposes, including assessment of disease-related anatomical changes and monitoring of articular and soft-tissue lesions over time, especially in response to urate-lowering therapy. Radiography remains the primary imaging technique. Ultrasonography may detect monosodium urate crystals on cartilage, is helpful to assess small joint effusion, to guide to joint aspiration, and to evaluate the volume of tophi. Computed tomography is considered to be more sensitive than plain radiography in the detection and evaluation of cortical bone erosions associated with tophi. MRI represents the only imaging modality which provides visualization of bone marrow oedema associated with erosions and may be useful to characterize and distinguish tophi from other soft tissue nodules

Single-cell gene network analysis and transcriptional landscape of MYCN-amplified neuroblastoma cell lines

Neuroblastoma (NBL) is a pediatric cancer responsible for more than 15% of cancer deaths in children, with 800 new cases each year in the United States alone. Genomic amplification of the MYC oncogene family member MYCN characterizes a subset of high-risk pediatric neuroblastomas. Several cellular models have been implemented to study this disease over the years. Two of these, SK-N-BE-2-C (BE2C) and Kelly, are amongst the most used worldwide as models of MYCN-Amplified human NBL. Here, we provide a transcriptome-wide quantitative measurement of gene expression and transcriptional network activity in BE2C and Kelly cell lines at an unprecedented single-cell resolution. We obtained 1105 Kelly and 962 BE2C unsynchronized cells, with an average number of mapped reads/cell of roughly 38,000. The single-cell data recapitulate gene expression signatures previously generated from bulk RNA-Seq. We highlight low variance for commonly used housekeeping genes between different cells (ACTB, B2M and GAPDH), while showing higher than expected variance for metallothionein transcripts in Kelly cells. The high number of samples, despite the relatively low read coverage of single cells, allowed for robust pathway enrichment analysis and master regulator analysis (MRA), both of which highlight the more mesenchymal nature of BE2C cells as compared to Kelly cells, and the upregulation of TWIST1 and DNAJC1 transcriptional networks. We further defined master regulators at the single cell level and showed that MYCN is not constantly active or expressed within Kelly and BE2C cells, independently of cell cycle phase. The dataset, alongside a detailed and commented programming protocol to analyze it, is fully shared and reusable

Efficacy of a coordinated strategy for containment of multidrug-resistant Gram-negative bacteria carriage in a Neonatal Intensive Care Unit in the context of an active surveillance program

Background: Antimicrobial resistance in neonatal intensive care unit (NICU) patients is a threat, due to the frequent use of antimicrobial treatment and invasive devices in fragile babies. Since 2014 an active surveillance program of multidrug-resistant Gram-negative bacteria (MDR-GNB) carriage has been in place in the five NICUs of Palermo, Italy. In 2017 an increase in the prevalence of MDR-GNB, and in particular of extended-spectrum β-lactamases-producing Klebsiella pneumoniae (ESBL-KP), was observed in “Civico” hospital NICU. Aim: To assess the impact of a coordinated intervention strategy in achieving long-lasting reduction of MDR-GNB prevalence in the NICU. Methods: Rectal swabs were obtained monthly and processed to detect MDR-GNB using standard methods. MDR-GNB were characterized by pulsed-field gel electrophoresis (PFGE). Since November 2017 the following intervention measures were applied: (a) two-months intensification of sample collection; (b) stakeholders meetings; (c) improvement of prevention measures and antimicrobial policies. Findings: During the intensified microbiological surveillance MDR-GNB and ESBL-KP were detected in rectal swabs (34.8%; 23.2%), nasal swabs (24.6%; 14.5%), oral swabs (14.5%; 5.4%), milk samples (32.1%; 17.9%), pacifiers swabs (30.8%; 17.9%) and from sub-intensive room surfaces. Thirteen ESBL-KP strains isolated from clinical and environmental samples showed identical PFGE patterns. The prevalence of MDR-GNB and ESBL-KP carriage significantly decreased in the year after intervention compared to the previous year (20.6% vs 62.2%; p < 0.001 and 11.1% vs 57.8%; p < 0.001). MDR-GNB were not detected at all for three months and ESBL-KP for five months. Multivariate analysis of the principal exposure variables showed that admission in the post-intervention period significantly reduced the risk of MDR-GNB carriage (adj-OR = 0.21, 95% CI = 0.076–0.629; p < 0.001). Conclusions: MDR-GNB broadly circulate in NICU setting, they can colonize different body sites and spread through various vehicles. A coordinated strategy of multiple interventions with active cooperation between epidemiologists and clinicians in the NICU can effectively reduce their circulation and in particular the carriage of the most dangerous ESBL-KP strains

Supernova search with active learning in ZTF DR3

We provide the first results from the complete SNAD adaptive learning pipeline in the context of a broad scope of data from large-scale astronomical surveys. The main goal of this work is to explore the potential of adaptive learning techniques in application to big data sets. Our SNAD team used Active Anomaly Discovery (AAD) as a tool to search for new supernova (SN) candidates in the photometric data from the first 9.4 months of the Zwicky Transient Facility (ZTF) survey, namely, between March 17 and December 31 2018 (58194 < MJD < 58483). We analysed 70 ZTF fields at a high galactic latitude and visually inspected 2100 outliers. This resulted in 104 SN-like objects being found, 57 of which were reported to the Transient Name Server for the first time and with 47 having previously been mentioned in other catalogues, either as SNe with known types or as SN candidates. We visually inspected the multi-colour light curves of the non-catalogued transients and performed fittings with different supernova models to assign it to a probable photometric class: Ia, Ib/c, IIP, IIL, or IIn. Moreover, we also identified unreported slow-evolving transients that are good superluminous SN candidates, along with a few other non-catalogued objects, such as red dwarf flares and active galactic nuclei. Beyond confirming the effectiveness of human-machine integration underlying the AAD strategy, our results shed light on potential leaks in currently available pipelines. These findings can help avoid similar losses in future large-scale astronomical surveys. Furthermore, the algorithm enables direct searches of any type of data and based on any definition of an anomaly set by the expert.Comment: 22 pages with appendix, 12 figures, 2 tables, accepted for publication in Astronomy and Astrophysic

An update of the evolving epidemic of blaKPC carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of multiple Non-ST258 Clones

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute general hospitals in Palermo, Italy, in the six-month period March-August 2014. They were characterized by PCR for β-lactam, aminoglycoside and plasmid mediated fluoroquinolone resistance genetic determinants. The mgrB gene of the colistin resistant isolates was amplified and sequenced. Clonality was assessed by pulsed field gel electrophoresis and multilocus sequence typing. Eight non-CC258 sequence types (STs) were identified accounting for 60% of isolates. In particular, ST307 and ST273 accounted for 29% and 18% of isolates. CC258 isolates were more frequently susceptible to gentamicin and non-CC258 isolates to amikacin. Colistin non susceptibility was found in 42% of isolates. Modifications of mgrB were found in 32 isolates. Conclusions: Concurrent clonal expansion of some STs and lateral transmission of genetic resistance determinants are likely producing a thorough change of the KPC-Kp epidemiology in Palermo, Italy. In our setting mgrB inactivation proved to substantially contribute to colistin resistance. Our findings suggest the need to continuously monitor the KPC-Kp epidemiology and to assess by a nationwide survey the possible shifting towards a polyclonal epidemic

An update of the evolving epidemic of blaKPC carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of multiple Non-ST258 Clones

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute general hospitals in Palermo, Italy, in the six-month period March-August 2014. They were characterized by PCR for β-lactam, aminoglycoside and plasmid mediated fluoroquinolone resistance genetic determinants. The mgrB gene of the colistin resistant isolates was amplified and sequenced. Clonality was assessed by pulsed field gel electrophoresis and multilocus sequence typing. Eight non-CC258 sequence types (STs) were identified accounting for 60% of isolates. In particular, ST307 and ST273 accounted for 29% and 18% of isolates. CC258 isolates were more frequently susceptible to gentamicin and non-CC258 isolates to amikacin. Colistin non susceptibility was found in 42% of isolates. Modifications of mgrB were found in 32 isolates. Conclusions: Concurrent clonal expansion of some STs and lateral transmission of genetic resistance determinants are likely producing a thorough change of the KPC-Kp epidemiology in Palermo, Italy. In our setting mgrB inactivation proved to substantially contribute to colistin resistance. Our findings suggest the need to continuously monitor the KPC-Kp epidemiology and to assess by a nationwide survey the possible shifting towards a polyclonal epidemic

Scaling and root planing with and without periodontal flap surgery

. Complete removal of calculus is a primary part of achieving a “biologically acceptable” tooth surface in the treatment of periodontitis. Rabbani et al. reported that a single episode of scaling did not completely remove subgingival calculus and that the deeper the periodontal pocket, the less complete the calculus removal. The purpose of the present study was to evaluate the effectiveness of scaling relative to calculus removal following reflection of a periodontal flap. Each of 21 patients who required multiple extractions had 2 teeth scaled, 2 teeth scaled following the reflection of a periodontal flap, and 2 teeth serve as controls. Local anesthesia was used. Following extraction, the % of subgingival tooth surfaces free of calculus was determined using the method described by Rabbani with a stereomicroscope. Results showed that while scaling only (SO) and scaling with a flap (SF) increased the % of root surface without calculus, scaling following the reflection of a flap aided calculus removal in pockets 4 mm and deeper. Comparison of SO versus SF at various pocket depths for % of tooth surfaces completely free of calculus showed 1 to 3 mm pockets to be 86% versus 86%, 4 to 6 mm pockets to be 43% versus 76% and >6 mm pockets to be 32% versus 50%. The extent of residual calculus was directly related to pocket depth, was greater following scaling only, and was greatest at the CEJ or in association with grooves, fossae or furcations. No differences were noted between anterior and posterior teeth or between different tooth surfaces.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73823/1/j.1600-051X.1986.tb01461.x.pd