204 research outputs found
Myosin tails and single α-helical domains
The human genome contains 39 myosin genes, divided up into 12 different classes. The structure, cellular function and biochemical properties of many of these isoforms remain poorly characterized and there is still some controversy as to whether some myosin isoforms are monomers or dimers. Myosin isoforms 6 and 10 contain a stable single α-helical (SAH) domain, situated just after the canonical lever. The SAH domain is stiff enough to be able to lengthen the lever allowing the myosin to take a larger step. In addition, atomic force microscopy and atomistic simulations show that SAH domains unfold at relatively low forces and have a high propensity to refold. These properties are likely to be important for protein function, enabling motors to carry cargo in dense actin networks, and other proteins to remain attached to binding partners in the crowded cell
Stable single α-Helices are constant force springs in proteins
Single α-helix (SAH) domains are rich in charged residues (Arg, Lys, and Glu) and stable in solution over a wide range of pH and salt concentrations. They are found in many different proteins where they bridge two functional domains. To test the idea that their high stability might enable these proteins to resist unfolding along their length, the properties and unfolding behavior of the predicted SAH domain from myosin-10 were characterized. The expressed and purified SAH domain was highly helical, melted non-cooperatively, and was monomeric as shown by circular dichroism and mass spectrometry as expected for a SAH domain. Single molecule force spectroscopy experiments showed that the SAH domain unfolded at very low forces (<30 pN) without a characteristic unfolding peak. Molecular dynamics simulations showed that the SAH domain unfolds progressively as the length is increased and refolds progressively as the length is reduced. This enables the SAH domain to act as a constant force spring in the mechanically dynamic environment of the cell
Elucidating the structural composition of a Fe-N-C catalyst by nuclear and electron resonance techniques
FeâNâC catalysts are very promising materials for fuel cells and metalâair batteries. This work gives fundamental insights into the structural composition of an FeâNâC catalyst and highlights the importance of an inâdepth characterization. By nuclearâ and electronâresonance techniques, we are able to show that even after mild pyrolysis and acid leaching, the catalyst contains considerable fractions of αâiron and, surprisingly, iron oxide. Our work makes it questionable to what extent FeN4 sites can be present in FeâNâC catalysts prepared by pyrolysis at 900â°C and above. The simulation of the iron partial density of phonon states enables the identification of three FeN4 species in our catalyst, one of them comprising a sixfold coordination with endâon bonded oxygen as one of the axial ligands
Identification of \u3ci\u3ePlanktothrix\u3c/i\u3e (Cyanobacteria) Blooms and Effects on the Aquatic Macroinvertebrate Community in the Non-Tidal Potomac River, USA
Using transverse cross-sectional transects, a survey of 31 km of the non-tidal Potomac River was conducted from Whiteâs Ferry, Virginia to Brunswick, Maryland, USA, between June and September in 2013 through 2015 to assess a recurring benthic cyanobacteria bloom. Abundant benthic cyanobacteria blooms were detected during the 2014 and 2015 sampling seasons and the primary taxon was identified morphologically and molecularly as Planktothrix cf. isothrix. When present, P. cf. isothrix blooms were concentrated from river center to the Maryland shoreline. This pattern was correlated with significantly greater benthic chlorophyll-a and phycocyanin concentrations. In an apparent response to the P. cf. isothrix blooms in the study site, aquatic macroinvertebrate community assemblages were significantly different between areas with extensive benthic cyanobacterial growth compared to areas without cyanobacterial growth. Within the P. cf. isothrix mats, the percentage of pollution sensitive taxa was lower and the percentage of pollution tolerant taxa was greater. These data suggest that P. cf. isothrix can act as an ecosystem disruptor through direct impacts to the aquatic macroinvertebrate abundance and community structure within this section of the freshwater, non-tidal Potomac River
Characterization of long and stable de novo single alpha-helix domains provides novel insight into their stability
Naturally-occurring single α-helices (SAHs), are rich in Arg (R), Glu (E) and Lys (K) residues, and stabilized by multiple salt bridges. Understanding how salt bridges promote their stability is challenging as SAHs are long and their sequences highly variable. Thus, we designed and tested simple de novo 98-residue polypeptides containing 7-residue repeats (AEEEXXX, where X is K or R) expected to promote salt-bridge formation between Glu and Lys/Arg. Lys-rich sequences (EK3 (AEEEKKK) and EK2R1 (AEEEKRK)) both form SAHs, of which EK2R1 is more helical and thermo-stable suggesting Arg increases stability. Substituting Lys with Arg (or vice versa) in the naturally-occurring myosin-6 SAH similarly increased (or decreased) its stability. However, Arg-rich de novo sequences (ER3 (AEEERRR) and EK1R2 (AEEEKRR)) aggregated. Combining a PDB analysis with molecular modelling provides a rational explanation, demonstrating that Glu and Arg form salt bridges more commonly, utilize a wider range of rotamer conformations, and are more dynamic than Glu-Lys. This promiscuous nature of Arg helps explain the increased propensity of de novo Arg-rich SAHs to aggregate. Importantly, the specific K:R ratio is likely to be important in determining helical stability in de novo and naturally-occurring polypeptides, giving new insight into how single α-helices are stabilized
DFT Calculations as a Tool to Analyse Quadrupole Splittings of Spin Crossover Fe(II) complexes
Density functional methods have been applied to calculate the quadrupole
splitting of a series of iron(II) spin crossover complexes. Experimental and
calculated values are in reasonable agreement. In one case spin-orbit coupling
is necessary to explain the very small quadrupole splitting value of 0.77 mm/s
at 293 K for a high-spin isomer
Dynamic ion pair behavior stabilizes single alpha helices in proteins.
Ion pairs are key stabilizing interactions between oppositely charged amino acid side chains in proteins. They are often depicted as single conformer salt bridges (hydrogen-bonded ion pairs) in crystal structures, but it is unclear how dynamic they are in solution. Ion pairs are thought to be particularly important in stabilizing single α-helix (SAH) domains in solution. These highly stable domains are rich in charged residues (such as Arg, Lys, and Glu) with potential ion pairs across adjacent turns of the helix. They provide a good model system to investigate how ion pairs can contribute to protein stability. Using NMR spectroscopy, small-angle X-ray light scattering (SAXS), and molecular dynamics simulations, we provide here experimental evidence that ion pairs exist in a SAH in murine myosin 7a (residues 858-935), but that they are not fixed or long lasting. In silico modeling revealed that the ion pairs within this α-helix exhibit dynamic behavior, rapidly forming and breaking and alternating between different partner residues. The low-energy helical state was compatible with a great variety of ion pair combinations. Flexible ion pair formation utilizing a subset of those available at any one time avoided the entropic penalty of fixing side chain conformations, which likely contributed to helix stability overall. These results indicate the dynamic nature of ion pairs in SAHs. More broadly, thermodynamic stability in other proteins is likely to benefit from the dynamic behavior of multi-option solvent-exposed ion pairs
A robust, scanning quantum system for nanoscale sensing and imaging
Controllable atomic-scale quantum systems hold great potential as sensitive
tools for nanoscale imaging and metrology. Possible applications range from
nanoscale electric and magnetic field sensing to single photon microscopy,
quantum information processing, and bioimaging. At the heart of such schemes is
the ability to scan and accurately position a robust sensor within a few
nanometers of a sample of interest, while preserving the sensor's quantum
coherence and readout fidelity. These combined requirements remain a challenge
for all existing approaches that rely on direct grafting of individual solid
state quantum systems or single molecules onto scanning-probe tips. Here, we
demonstrate the fabrication and room temperature operation of a robust and
isolated atomic-scale quantum sensor for scanning probe microscopy.
Specifically, we employ a high-purity, single-crystalline diamond nanopillar
probe containing a single Nitrogen-Vacancy (NV) color center. We illustrate the
versatility and performance of our scanning NV sensor by conducting
quantitative nanoscale magnetic field imaging and near-field single-photon
fluorescence quenching microscopy. In both cases, we obtain imaging resolution
in the range of 20 nm and sensitivity unprecedented in scanning quantum probe
microscopy
Characterization of long and stable de novo single alpha-helix domains provides novel insight into their stability
Naturally-occurring single α-helices (SAHs), are rich in Arg (R), Glu (E) and Lys (K) residues, and stabilized by multiple salt bridges. Understanding how salt bridges promote their stability is challenging as SAHs are long and their sequences highly variable. Thus, we designed and tested simple de novo 98-residue polypeptides containing 7-residue repeats (AEEEXXX, where X is K or R) expected to promote salt-bridge formation between Glu and Lys/Arg. Lys-rich sequences (EK3 (AEEEKKK) and EK2R1 (AEEEKRK)) both form SAHs, of which EK2R1 is more helical and thermo-stable suggesting Arg increases stability. Substituting Lys with Arg (or vice versa) in the naturally-occurring myosin-6 SAH similarly increased (or decreased) its stability. However, Arg-rich de novo sequences (ER3 (AEEERRR) and EK1R2 (AEEEKRR)) aggregated. Combining a PDB analysis with molecular modelling provides a rational explanation, demonstrating that Glu and Arg form salt bridges more commonly, utilize a wider range of rotamer conformations, and are more dynamic than GluâLys. This promiscuous nature of Arg helps explain the increased propensity of de novo Arg-rich SAHs to aggregate. Importantly, the specific K:R ratio is likely to be important in determining helical stability in de-novo and naturally-occurring polypeptides, giving new insight into how single α-helices are stabilized
Leveraging multimission satellite data for spatiotemporally coherent cyanoHAB monitoring
Cyanobacteria harmful algal blooms (cyanoHABs) present a critical public health challenge for aquatic resource and public health managers. Satellite remote sensing is well-positioned to aid in the identification and mapping of cyanoHABs and their dynamics, giving freshwater resource managers a tool for both rapid and long-term protection of public health. Monitoring cyanoHABs in lakes and reservoirs with remote sensing requires robust processing techniques for generating accurate and consistent products across local and global scales at high revisit rates. We leveraged the high spatial and temporal resolution chlorophyll-a (Chla) and phycocyanin (PC) maps from two multispectral satellite sensors, the Sentinel-2 (S2) MultiSpectral Instrument (MSI) and the Sentinel-3 (S3) Ocean Land Colour Instrument (OLCI) respectively, to study bloom dynamics in Utah Lake, United States, for 2018. We used established Mixture Density Networks (MDNs) to map Chla from MSI and train new MDNs for PC retrieval from OLCI, using the same architecture and training dataset previously proven for PC retrieval from hyperspectral imagery. Our assessment suggests lower median uncertainties and biases (i.e., 42% and -4%, respectively) than that of existing top-performing PC algorithms. Additionally, we compared bloom trends in MDN-based PC and Chla products to those from a satellite-derived cyanobacteria cell density estimator, the cyanobacteria index (CI-cyano), to evaluate their utility in the context of public health risk management. Our comprehensive analyses indicate increased spatiotemporal coherence of bloom magnitude, frequency, occurrence, and extent of MDN-based maps compared to CI-cyano and potential for use in cyanoHAB monitoring for public health and aquatic resource managers
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