Acute Ultraviolet Light Exposure and Post-Resistance Exercise Serum Testosterone: A Pilot Study in Older Men

International Journal of Exercise Science 13(2): 607-614, 2020. Testosterone deficiency is linked to a multitude of conditions including depression, sexual dysfunction, and cognitive impairment. Although synthetic testosterone-boosting pharmaceuticals have gained wide use, recent data suggests that vitamin D, ingested orally, may positively impact serum testosterone. Furthermore, evidence tying ultraviolet (UV) light exposure to endogenous vitamin D synthesis presents an intriguing possibility for naturally enhancing serum testosterone. This investigation sought to determine the effect of acute UV light exposure on resistance exercise-induced acute testosteronemia and vitamin D in older men. Six older adult men of varying activity levels (age 62 ± 1.79 yrs., height 179.92 ± 1.12 cm., body mass 83.79 ± 3.12 kg., BMI 25.95 ± 1.15 kg/m2) participated in two testing sessions separated by one week: 1) Resistance exercise followed by standard passive recovery (RERC) and 2) RE plus UV light exposure during the first 10-minutes of RE passive recovery (RERC-UV). The RE protocol was identical in both sessions and consisted of four sets of 10RM on leg press, chest press, and back row with 1-minute of rest between sets followed by 30-minutes of post-RE passive recovery. Serum testosterone and vitamin D were measured pre- and post-RE in 5-minute increments during the 30-minute recovery. Analysis of variance revealed neither RE or RERC-UV significantly affected serum testosterone or vitamin D. These findings suggest that acute UV light exposure may be insufficient to positively impact serum testosterone and vitamin D following a single bout of RE in older adult men

Molecular Study of Sheep Malignant Theileriosis at Barka Region in the Sultanate of Oman

Background: We used the PCR technique based on the abovementioned primer pair and sequenc­ing to demonstrate the Theileria infection in the sheep samples collected from Sultanate of Oman.Methods: According to the frame work of "integrated control of ticks and tick borne diseases in global­ized world managed by EU-ICTTD-3 project, the samples from blood, liver, spleen, lymph node and lung were sent to the laboratory of Iranian Research Center for Ticks and Tick-borne Diseases (IRCTTD). Sam­ples from blood smear and impression smears from liver, spleen, lymph node, and lung were analyzed by Geimsa staining. The DNA was extracted from the abovemen­tioned samples and analyzed by PCR tech­nique using specific primers derived from the nucleo­tide sequences of 18S rRNA gene of T. lestoquardi, which can amplify the common region in other Theileria and Babesia spp. Subsequently the amplified DNA was sequenced.Results: The analysis of blood smears of the sheep was negative for piroplasmosis performed through the Giemsa staining. The impression smears prepared from liver, spleen, lymph node, and lung showed suspi­cious structures mimicking Theileria schizonts in some cells. The results showed an expected PCR prod­uct of 428 bp in length, which is specific for Theileria spp. The PCR products were subsequently se­quenced. The corresponding nucleotide sequence is registered under accession number JF309152 in Gen­Bank. The sequence alignment in GenBank showed that the PCR products had 99% homology to the known T. lestoquardi registered under accession number AF081135 in the GenBank. Conclusion: Oman sheep are highly susceptible for Theileria infection and the infected sheep mostly die before the microschizonts or erythrocytic form of Theileria appears in the nucleated or ery­trocytic cells respectively

Wind Tunnel Model Design for Sonic Boom Studies of Nozzle Jet with Shock Interactions

NASA and Industry are performing vehicle studies of configurations with low sonic boom pressure signatures. The computational analyses of modern configuration designs have matured to the point where there is confidence in the prediction of the pressure signature from the front of the vehicle, but uncertainty in the aft signatures with often greater boundary layer effects and nozzle jet pressures. Wind tunnel testing at significantly lower Reynolds numbers than in flight and without inlet and nozzle jet pressures make it difficult to accurately assess the computational solutions of flight vehicles. A wind tunnel test in the NASA Ames 9- by 7-Foot Supersonic Wind Tunnel from Mach 1.6 to 2.0 will be used to assess the effects of shocks from components passing through nozzle jet plumes on the sonic boom pressure signature and provide datasets for comparison with CFD codes. A large number of high-fidelity numerical simulations of wind tunnel test models with a variety of shock generators that simulate horizontal tails and aft decks have been studied to provide suitable models for sonic boom pressure measurements using a minimally intrusive pressure rail in the wind tunnel. The computational results are presented and the evolution of candidate wind tunnel models is summarized and discussed in this paper

Immunocompetent murine models for the study of glioblastoma immunotherapy.

Glioblastoma remains a lethal diagnosis with a 5-year survival rate of less than 10%. (NEJM 352:987-96, 2005) Although immunotherapy-based approaches are capable of inducing detectable immune responses against tumor-specific antigens, improvements in clinical outcomes are modest, in no small part due to tumor-induced immunosuppressive mechanisms that promote immune escape and immuno-resistance. Immunotherapeutic strategies aimed at bolstering the immune response while neutralizing immunosuppression will play a critical role in improving treatment outcomes for glioblastoma patients. In vivo murine models of glioma provide an invaluable resource to achieving that end, and their use is an essential part of the preclinical workup for novel therapeutics that need to be tested in animal models prior to testing experimental therapies in patients. In this article, we review five contemporary immunocompetent mouse models, GL261 (C57BL/6), GL26 (C57BL/6) CT-2A (C57BL/6), SMA-560 (VM/Dk), and 4C8 (B6D2F1), each of which offer a suitable platform for testing novel immunotherapeutic approaches

Collaborative multidisciplinary learning : quantity surveying students’ perspectives

The construction industry is highly fragmented and is known for its adversarial culture, culminating in poor quality projects not completed on time or within budget. The aim of this study is thus to guide the design of QS programme curricula in order to help students develop the requisite knowledge and skills to work more collaboratively in their multi-disciplinary future workplaces. A qualitative approach was considered appropriate as the authors were concerned with gathering an initial understanding of what students think of multi-disciplinary learning. The data collection method used was a questionnaire which was developed by the Behaviours4Collaboration (B4C) team. Knowledge gaps were still found across all the key areas where a future QS practitioner needs to be collaborative (either as a project contributor or as a project leader) despite the need for change instigated by the multi-disciplinary (BIM) education revolution. The study concludes that universities will need to be selective in teaching, and innovative in reorienting, QS education so that a collaborative BIM education can be effected in stages, increasing in complexity as the students’ technical knowledge grows. This will help students to build the competencies needed to make them future leaders. It will also support programme currency and delivery

Artificial Intelligence-Enabled Optimization of Battery-Grade Lithium Carbonate Production

By 2035, the need for battery-grade lithium is expected to quadruple. About half of this lithium is currently sourced from brines and must be converted from a chloride into lithium carbonate (Li2CO3) through a process called softening. Conventional softening methods using sodium or potassium salts contribute to carbon emissions during reagent mining and battery manufacturing, exacerbating global warming. This study introduces an alternative approach using carbon dioxide (CO2(g)) as the carbonating reagent in the lithium softening process, offering a carbon capture solution. We employed an active learning-driven high-throughput method to rapidly capture CO2(g) and convert it to lithium carbonate. The model was simplified by focusing on the elemental concentrations of C, Li, and N for practical measurement and tracking, avoiding the complexities of ion speciation equilibria. This approach led to an optimized lithium carbonate process that capitalizes on CO2(g) capture and improves the battery metal supply chain's carbon efficiency

Wind Tunnel Model Design for Sonic Boom Studies of Nozzle Jet Flows with Shock Interactions

NASA and the U.S. aerospace industry are performing studies of supersonic aircraft concepts with low sonic boom pressure signatures. The computational analyses of modern aircraft designs have matured to the point where there is confidence in the prediction of the pressure signature from the front of the vehicle, but uncertainty remains in the aft signatures due to boundary layer and nozzle exhaust jet effects. Wind tunnel testing without inlet and nozzle exhaust jet effects at lower Reynolds numbers than in-flight make it difficult to accurately assess the computational solutions of flight vehicles. A wind tunnel test in the NASA Ames 9- by 7-Foot Supersonic Wind Tunnel is planned for February 2016 to address the nozzle jet effects on sonic boom. The experiment will provide pressure signatures of test articles that replicate waveforms from aircraft wings, tails, and aft fuselage (deck) components after passing through cold nozzle jet plumes. The data will provide a variety of nozzle plume and shock interactions for comparison with computational results. A large number of high-fidelity numerical simulations of a variety of shock generators were evaluated to define a reduced collection of suitable test models. The computational results of the candidate wind tunnel test models as they evolved are summarized, and pre-test computations of the final designs are provided

Electroactive Molecularly Imprinted Polymer Nanoparticles (eMIPs) for Label‐free Detection of Glucose: Toward Wearable Monitoring

The diagnosis of diabetes mellitus (DM) affecting 537 million adults worldwide relies on invasive and costly enzymatic methods that have limited stability. Electroactive polypyrrole (PPy)-based molecularly imprinted polymer nanoparticles (eMIPs) have been developed that rival the affinity of enzymes whilst being low-cost, highly robust, and facile to produce. By drop-casting eMIPs onto low-cost disposable screen-printed electrodes (SPEs), sensors have been manufactured that can electrochemically detect glucose in a wide dynamic range (1 µm–10 mm) with a limit of detection (LOD) of 26 nm. The eMIPs sensors exhibit no cross reactivity to similar compounds and negligible glucose binding to non-imprinted polymeric nanoparticles (eNIPs). Measurements of serum samples of diabetic patients demonstrate excellent correlation (>0.93) between these eMIPs sensor and the current gold standard Roche blood analyzer test. Finally, the eMIPs sensors are highly durable and reproducible (storage >12 months), showcasing excellent robustness and thermal and chemical stability. Proof-of-application is provided via measuring glucose using these eMIPs sensor in a two-electrode configuration in spiked artificial interstitial fluid (AISF), highlighting its potential for non-invasive wearable monitoring. Due to the versatility of the eMIPs that can be adapted to virtually any target, this platform technology holds high promise for sustainable healthcare applications via providing rapid detection, low-cost, and inherent robustness

High-resolution 3D analysis of mouse small-intestinal stroma.

Here we detail a protocol for whole-mount immunostaining of mouse small-intestinal villi that can be used to generate high-resolution 3D images of all gut cell types, including blood and lymphatic vessel cells, neurons, smooth muscle cells, fibroblasts and immune cells. The procedure describes perfusion, fixation, dissection, immunostaining, mounting, clearing, confocal imaging and quantification, using intestinal vasculature as an example. As intestinal epithelial cells prevent visualization with some antibodies, we also provide an optional protocol to remove these cells before fixation. In contrast to alternative current techniques, our protocol enables the entire villus to be visualized with increased spatial resolution of cell location, morphology and cell-cell interactions, thus allowing for easy quantification of phenotypes. The technique, which takes 7 d from mouse dissection to microscopic examination, will be useful for researchers who are interested in most aspects of intestinal biology, including mucosal immunology, infection, nutrition, cancer biology and intestinal microbiota