102 research outputs found
Trastornos del desarrollo en niños y adolescentes de la región de Los Ríos, Valdivia, Chile, 2006-2008
ResumenObjetivoEstudiar los posibles hallazgos de factores asociados antenatales, perinatales o posnatales, tanto de la madre como del niño, que tengan relevancia significativa en la aparición de trastornos del desarrollo.Pacientes y métodoSe creó una base de datos de todas las fichas clínicas de los pacientes con patología del desarrollo F80 a F90 CIE10 ingresados a la Unidad de Psiquiatría Infantil del Hospital Regional de Valdivia entre agosto de 2006 y diciembre de 2008. Total: 493 pacientes (48,7% del total de consultantes); grupo control de 32 niños sanos de consultorios de la ciudad. Se aplicó método estadístico odds ratio (95% confianza) para el análisis de algunas variables.ResultadosDestacan como factores de riesgo para desarrollar patología del desarrollo (p<0,005, 25% de frecuencia en población consultante): la prematurez, ser de sexo masculino, tener una madre con baja escolaridad, hospitalizaciones tempranas y enfermedades médicas antes de los 3 años (todos con odds ratio significativa). Además tener una madre psiquiátrica aumenta el doble el riesgo de desarrollar trastorno del desarrollo.ConclusiónSe requiere un trabajo colaborativo interdisciplinario entre neonatólogos, obstetras, psiquiatras infantiles y la atención primaria para detectar precozmente niños en riesgo.AbstractObjectivesTo study possible findings of factors in the antenatal, perinatal or postnatal period, in the mother or the child that may have an influence on the appearance of a developmental disorder.Patients and methodA Data Base of Clinical Histories from every patient with a developmental disorder (F80-F90 ICD10) was created. The patients attended the Child Psychiatric Unit at Hospital Regional of Valdivia, Chile, from August 2006 to December 2008. Total: 493 patientes (48.7% of the total of patients consulting); Control group: 32 healthy patients. Statistical method: odds ratio (95% confidence).ResultsThe main risk factors for developing a developmental disorder (P<.005, 25% frequency in the consulting population) are: prematurity, male sex, mother with low education, early hospitalizations, and medical illnesses (all with a significant odds ratio). Also, having a mother with psychiatric illness doubles the risk of having a developmental disorder.ConclusionIt requires an interdisciplinary collaborative work between neonatologists, obstetricians, child psychiatrists and the primary care to detect early children at risk
Toxoplasma gondii in Meat for Human Consumption – A Brief Review of the Most Described Strategies for Its Detection and Quantification
Toxoplasmosis is a parasitic zoonotic disease widely distributed worldwide and is caused by the intracellular parasite Toxoplasma gondii. The definitive host of T. gondii is the domestic cat and the entire cat family, in which the sexual stages of the parasite develop. T. gondii can also infect a wide range of intermediate hosts, affecting most warm-blooded animals including humans. In humans, toxoplasmosis is usually asymptomatic in healthy individuals, but can develop lymphadenopathy and nonspecific symptomatology or even be fatal in infants with congenital toxoplasmosis and in immunocompromised patients. Transmission to humans is mainly through food, especially by eating undercooked meat or meat contaminated with tissue cysts. This has led to various public health organizations worldwide monitoring programs on T. gondii in animals intended for human consumption, especially in meat samples. One of the techniques employed in the laboratory is that based on the polymerase chain reaction and some of its variants, which have proven to be valuable tools for the detection of T. gondii in tissues for human consumption and many other types of biological samples. The development of different strategies for the molecular detection of T. gondii has led to the identification and quantification methodologies varying widely among laboratories. Therefore, this chapter reviews the main methods of extraction, purification, detection and quantification of T. gondii DNA in tissue samples from different species destined for human consumption
Sublittoral soft bottom communities and diversity of Mejillones Bay in northern Chile (Humboldt Current upwelling system)
The macrozoobenthos of Mejillones Bay (23°S; Humboldt Current) was quantitatively investigated over a 7-year period from austral summer 1995/1996 to winter 2002. About 78 van Veen grab samples taken at six stations (5, 10, 20 m depth) provided the basis for the analysis of the distribution of 60 species and 28 families of benthic invertebrates, as well as of their abundance and biomass. Mean abundance (2,119 individuals m-2) was in the same order compared to a previous investigation; mean biomass (966 g formalin wet mass m-2), however, exceeded prior estimations mainly due to the dominance of the bivalve Aulacomya ater. About 43% of the taxa inhabited the complete depth range. Mean taxonomic Shannon diversity (H', Log e) was 1.54 ± 0.58 with a maximum at 20 m (1.95 ± 0.33); evenness increased with depth. The fauna was numerically dominated by carnivorous gastropods, polychaetes and crustaceans (48%). About 15% of the species were suspensivorous, 13% sedimentivorous, 11% detritivorous, 7% omnivorous and 6% herbivorous. Cluster analyses showed a significant difference between the shallow and the deeper stations. Gammarid amphipods and the polychaete family Nephtyidae characterized the 5-mzone, the molluscs Aulacomya ater, Mitrella unifasciata and gammarids the intermediate zone, while the gastropod Nassarius gayi and the polychaete family Nereidae were most prominent at the deeper stations. The communities of the three depth zones did not appear to be limited by hypoxia during non-El Niño conditions. Therefore, no typical change in community structure occurred during El Niño 1997–1998, in contrast to what was observed for deeper faunal assemblages and hypoxic bays elsewhere in the coastal Humboldt Current system
Near-IR narrow-band imaging with CIRCE at the Gran Telescopio Canarias: Searching for Lyα-emitters at z ∼ 9.3
Context. Identifying very high-redshift galaxies is crucial for understanding the formation and evolution of galaxies. However, many questions still remain, and the uncertainty on the epoch of reionization is large. In this approach, some models allow a double-reionization scenario, although the number of confirmed detections at very high z is still too low to serve as observational proof. Aims: The main goal of this project is studying whether we can search for Lyman-α emitters (LAEs) at z ∼ 9 using a narrow-band (NB) filter that was specifically designed by our team and was built for this experiment. Methods: We used the NB technique to select candidates by measuring the flux excess due to the Lyα emission. The observations were taken with an NB filter (full width at half minimum of 11 nm and central wavelength λc = 1.257 μm) and the CIRCE near-infrared camera for the Gran Telescopio Canarias. We describe a data reduction procedure that was especially optimized to minimize instrumental effects. With a total exposure time of 18.3 h, the final NB image covers an area of ∼6.7 arcmin2, which corresponds to a comoving volume of 1.1 × 103 Mpc3 at z = 9.3. Results: We pushed the source detection to its limit, which allows us to analyze an initial sample of 97 objects. We detail the different criteria we applied to select the candidates. The criteria included visual verifications in different photometric bands. None of the objects resembled a reliable LAE, however, and we found no robust candidate down to an emission-line flux of 2.9 × 10−16 erg s−1 cm−2, which corresponds to a Lyα luminosity limit of 3 × 1044 erg s−1. We derive an upper limit on the Lyα luminosity function at z ∼ 9 that agrees well with previous constraints. We conclude that deeper and wider surveys are needed to study the LAE population at the cosmic dawn
CHICKEN COOPS, Triatoma dimidiata INFESTATION AND ITS INFECTION WITH Trypanosoma cruzi IN A RURAL VILLAGE OF YUCATAN, MEXICO
First results using a new near-infrared 1% narrow-band filter in the GTC 10.4m telescope to detect galaxies at the dawn of the universe
We present the first results using a new SCHOTT 1% narrow band filter in the near infrared for the CIRCE camera of the 10.4m GTC telescope. The goal of the project is to detect very distant galaxies at the dawn of the Universe. These remote and extremely faint galaxies are selected by their Ly-alpha emission. For this project SCHOTT manufactured a high transmission 11 nm narrow band filter which has been used in the fully cryogenic near-infrared camera CIRCE of the Gran Telescopio Canarias Telescope. A steep interference filter Bandpass with FWHM 11nm centered at 1254nm was coated on a fused silica substrate. The filter achieved excellent maximum transmission and deep out of band blocking. This was achieved by using magnetron sputtering for the filter coating process. We report on the spectral and interferometric results of the filter and the scientific results achieved with a first set of observations
Comparative serology techniques for the diagnosis of Trypanosoma cruzi infection in a rural population from the state of Querétaro, Mexico
Immunological diagnostic methods for Trypanosoma cruzi depend specifically on the presence of antibodies and parasitological methods lack sensitivity during the chronic and “indeterminate” stages of the disease. This study performed a serological survey of 1,033 subjects from 52 rural communities in 12 of the 18 municipalities in the state of Querétaro, Mexico. We detected anti-T. cruzi antibodies using the following tests: indirect haemagglutination assay (IHA), indirect immunofluorescence assay (IFA), ELISA and recombinant ELISA (rELISA). We also performed Western blot (WB) analysis using iron superoxide dismutase (FeSOD), a detoxifying enzyme excreted by the parasite, as the antigen. Positive test results were distributed as follows: ELISA 8%, rELISA 6.2%, IFA and IHA 5.4% in both cases and FeSOD 8%. A comparative study of the five tests was undertaken. Sensitivity levels, specificity, positive and negative predictive values, concordance percentage and kappa index were considered. Living with animals, trips to other communities, gender, age, type of housing and symptomatology at the time of the survey were statistically analysed using SPSS software v.11.5. Detection of the FeSOD enzyme that was secreted by the parasite and used as an antigenic fraction in WBs showed a 100% correlation with traditional ELISA tests
Synergistic Effect of Lupenone and Caryophyllene Oxide against Trypanosoma cruzi
The in vitro trypanocidal activity of a 1 : 4 mixture of lupenone and caryophyllene oxide confirmed a synergistic effect of the terpenoids against epimastigotes forms of T. cruzi (IC50=10.4 μg/mL, FIC = 0.46). In addition, testing of the terpenoid mixture for its capacity to reduce the number of amastigote nests in cardiac tissue and skeletal muscle of infected mice showed a reduction of more than 80% at a dose level of 20.8 mg·kg−1·day−1
Germination, seedling performance, and root production after simulated partial seed predation of a threatened Atacama Desert shrub
- …