Strain stiffening of peripheral nerves subjected to longitudinal extensions in vitro

The mechanical response of peripheral nerves is crucial to understand their physiological and pathological conditions. However, their response to external mechanical solicitations is still partially unclear, since peripheral nerves could behave in a quite complex way. In particular, nerves react to longitudinal strains increasing their stiffness to keep axons integrity and to preserve endoneural structures from overstretch. In this work, the strain stiffening of peripheral nerves was investigated in vitro through a recently introduced computational framework, which is able to theoretically reproduce the experimental behaviour of excised tibial and sciatic nerves. The evolution and the variation of the tangent modulus of tibial and sciatic nerve specimens were quantitatively investigated and compared to explore how stretched peripheral nerves change their instantaneous stiffness

Focusing properties of linear undulators

This paper investigates the focusing properties of linear magnetic undulators, i.e., devices characterized by weak defocusing properties in the horizontal (wiggling) plane and strongly focusing in the vertical plane. The problem of identifying the conditions that ensure the existence of the electron beam eigenstates in the undulator lattice for a given working point of electron beam energy E_{b} and resonant wavelength λ_{r} is studied. For any given undulator lattice, a bandlike structure is identified defining regions in the (E_{b},λ_{r}) plane where no periodic matching condition can be found, i.e., it is not possible to transport the electron beam so that optical functions are periodic at lattice boundaries. Some specific cases are discussed for the SPARC FEL undulator

impact of non gaussian electron energy heating upon the performance of a seeded free electron laser

E. Ferrari, E. Allaria, W. Fawley, L. Giannessi, Z. Huang, G. Penco, and S. Spampinati Elettra-Sincrotrone Trieste S.C.p.A. di interesse nazionale, Strada Statale 14-km 163,5 in AREA Science Park, 34149 Basovizza, Trieste, Italy Universita degli Studi di Trieste, Dipartimento di Fisica, Piazzale Europa 1, 34127 Trieste, Italy SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA Enea, via Enrico Fermi 45, 00044 Frascati, Roma, Italy Laboratory of Quantum Optics, University of Nova Gorica, 5000 Nova Gorica, Slovenia Department of Physics, University of Liverpool, Oxford Street L69 7ZE, Liverpool, United Kingdom Cockcroft Institute, Sci-Tech Daresbury, Keckwick Lane WA4 4AD, Daresbury, Warrington, United Kingdom (Received 11 October 2013; published 21 March 2014

FlhF, a signal recognition particle-like GTPase, is involved in the regulation of flagellar arrangement, motility behaviour and protein secretion in Bacillus cereus

Flagellar arrangement is a highly conserved feature within bacterial species. However, only a few genes regulating cell flagellation have been described in polar flagellate bacteria. This report demonstrates that the arrangement of flagella in the peritrichous flagellate Bacillus cereus is controlled by flhF. Disruption of flhF in B. cereus led to a reduction in the number of flagella from 10-12 to 1-3 filaments per cell in the insertion mutant MP06. Moreover, compared to the parental strain, MP06 exhibited: (i) shorter smooth swimming phases, causing reduced swimming motility but not affecting chemotaxis; (ii) complete inhibition of swarming motility, as differentiated swarm cells were never detected; (iii) an increased amount of extracellular proteins; and (iv) differential export of virulence determinants, such as haemolysin BL (HBL), phosphatidylcholine-preferring phospholipase C (PC-PLC) and non-haemolytic enterotoxin (NHE). Introduction of a plasmid harbouring flhF (pDGflhF) into MP06 completely restored the wild-type phenotype in the trans-complemented strain MP07. B. cereus flhF was found to constitute a monocistronic transcriptional unit and its overexpression did not produce abnormal features in the wild-type background. Characterization of a B. cereus mutant (MP05) carrying a partial flhF deletion indicated that the last C-terminal domain of FlhF is involved in protein export while not required for flagellar arrangement and motility behaviour. Taken together, these data suggest that B. cereus FlhF is a promising candidate for connecting diverse cellular functions, such as flagellar arrangement, motility behaviour, pattern of protein secretion and virulence phenotype

Gaseous argon time projection chamber with electroluminescence enhanced optical readout

Systematic uncertainties in accelerator oscillation neutrino experiments arise mostly from nuclear models describing neutrino-nucleus interactions. To mitigate these uncertainties, we can study neutrino-nuclei interactions with detectors possessing enhanced hadron detection capabilities at energies below the nuclear Fermi level. Gaseous detectors not only lower the particle detection threshold but also enable the investigation of nuclear effects on various nuclei by allowing for changes in the gas composition. This approach provides valuable insights into the modelling of neutrino-nucleus interactions and significantly reduces associated uncertainties. Here, we discuss the design and first operation of a gaseous argon time projection chamber optically read. The detector operates at atmospheric pressure and features a single stage of electron amplification based on a thick GEM. Here, photons are produced with wavelengths in the vacuum ultraviolet regime. In an optical detector the primary constraint is the light yield. This study explores the possibility of increasing the light yield by applying a low electric field downstream of the ThGEM. In this region, called the electroluminescence gap, electrons propagate and excite the argon atoms, leading to the subsequent emission of photons. This process occurs without any further electron amplification, and it is demonstrated that the total light yield increases up to three times by applying moderate electric fields of the order of 3~kV/cm. Finally, an indirect method is discussed for determining the photon yield per charge gain of a ThGEM, giving a value of 18.3 photons detected per secondary electron

Observation and Control of Laser-Enabled Auger Decay

Single photon laser enabled Auger decay (spLEAD) has been redicted theoretically [Phys. Rev. Lett. 111, 083004 (2013)] and here we report its first experimental observation in neon. Using coherent, bichromatic free-electron laser pulses, we have detected the process and coherently controlled the angular distribution of the emitted electrons by varying the phase difference between the two laser fields. Since spLEAD is highly sensitive to electron correlation, this is a promising method for probing both correlation and ultrafast hole migration in more complex systems.Comment: 5 pages, 3 figure

The ARC-EN-CIEL radiation sources

MOPC005International audienceThe ARC-EN-CIEL (Accelerator-Radiation for Enhanced Coherent Intense Extended Light) project proposes a panoply of light sources for the scientific community on a 1 GeV superconducting LINAC (phase 2) on which two ERL loops (1 and 2 GeV) are added in phase 3. LEL1 (200-1.5 nm), LEL2 (10-0.5 nm) and LEL4 (2-0.2 nm) are three kHz High Gain Harmonic Generation Free Electron Laser sources seeded with the High order Harmonics generated in Gas, with 100-30 FWHM pulses. A collaboration, which has been set-up with the SCSS Prototype Accelerator in Japan to test this key concept of ARC-EN-CIEL, has led to the experimental demonstration of the seeding with HHG and the observation up the 7th non linear harmonic with a seed at 160 nm. LEL3 (40-8 nm) installed on the 1 GeV loop is a MHz FEL oscillator providing higher average power and brilliance. In addition, in vacuum undulator spontaneous emission source extend the spectral range above 10 keV and intense THz radiation is generated by edge radiation of bending magnets. Optimisations and light sources characteristics are described

Seeded x-ray free-electron laser generating radiation with laser statistical properties

The invention of optical lasers led to a revolution in the field of optics and even to the creation of completely new fields of research such as quantum optics. The reason was their unique statistical and coherence properties. The newly emerging, short-wavelength free-electron lasers (FELs) are sources of very bright coherent extreme-ultraviolet (XUV) and x-ray radiation with pulse durations on the order of femtoseconds, and are presently considered to be laser sources at these energies. Most existing FELs are highly spatially coherent but in spite of their name, they behave statistically as chaotic sources. Here, we demonstrate experimentally, by combining Hanbury Brown and Twiss (HBT) interferometry with spectral measurements that the seeded XUV FERMI FEL-2 source does indeed behave statistically as a laser. The first steps have been taken towards exploiting the first-order coherence of FELs, and the present work opens the way to quantum optics experiments that strongly rely on high-order statistical properties of the radiation.Comment: 24 pages, 10 figures, 37 reference

Two-colour generation in a chirped seeded Free-Electron Laser

We present the experimental demonstration of a method for generating two spectrally and temporally separated pulses by an externally seeded, single-pass free-electron laser operating in the extreme-ultraviolet spectral range. Our results, collected on the FERMI@Elettra facility and confirmed by numerical simulations, demonstrate the possibility of controlling both the spectral and temporal features of the generated pulses. A free-electron laser operated in this mode becomes a suitable light source for jitter-free, two-colour pump-probe experiments

Histochemical characterisation and gene expression analysis of skeletal muscles from maremmana and aubrac steers reared on grazing and feedlot systems

This study aimed to characterise the fibre composition of Triceps brachii (TB) and Semimem-branosus (SM) muscles from 20 Maremmana (MA) and 20 Aubrac (AU) steers, and the effect of grazing activity in comparison with feedlot system. The histochemical method was performed with the m-ATPase method with an acid pre-incubation, thus allowing to distinguish type I, IIA, and IIB fibres. Additionally, on total RNA extracted from SM muscle, the expressions of atp1a1, mt-atp6, and capn1 genes were evaluated, in order to find potential associations with muscle fibre histochemical characteristics. In SM muscle, the MA steers had the greater frequency of oxidative fibres (type I and IIA) and the higher atp1a1 expression, in comparison to AU steers. Conversely, AU steers had a greater frequency of type IIB fibres, and the higher capn1 expression. A similar histochemical pattern was observed in TB muscle. The grazing activity was probably insufficient to determine differences both for fibre proportion and size, and gene expressions, except for mt-atp6 expression that was surprisingly highest in feedlot MA in comparison to other steers. These findings further the knowledge of muscle properties belonging to these breeds, and the effect of voluntary physical activity since few studies were available in this regard