In Vitro Effect of Molasses Concentration, pH, and Time on Chromium Removal by Trichoderma spp. from the Effluents of a Peruvian Tannery

The effluents generated by the tannery industry have a high content of chromium and other toxic elements, representing a potential threat to ecosystems. An eco-friendly alternative to treat these effluents is the use of microorganisms, such as fungi, with the capacity to biosorb heavy metals. The present work aims to determine the effect of the molasses concentration, pH variation, and time on the removal of total chromium using the filamentous fungus Trichoderma spp. An experimental design was adopted using pH (4 and 6), concentrations of molasses (0.5 and 1%), and time (8 and 12 days) as independent variables. The Trichoderma inoculum was constant in all the treatments. The different treatments were evaluated after 0, 8, and 12 days by taking 50 mL of sample from each bioreactor. The chromium concentration was subsequently determined in each sample. The results show that treatment 3 (1% molasses and pH 4) showed higher chromium removal after both 8 and 12 days. The concentrations of total chromium decreased from 665 mg/mL to values of 568 mg/mL by day 8 and 486 mg/mL by day 12. These values are, however, still above the maximum threshold imposed by Peruvian law regarding the discharge of non-domestic effluents into the sewage system. The results show that Trichoderma spp. can increasingly remove chromium from the effluent with longer incubation periods. However, future studies are necessary to determine the mechanisms of chromium biosorption by the fungus and the influence of other physicochemical parameters

In Vitro Effect of Molasses Concentration, pH, and Time on Chromium Removal by Trichoderma spp. from the Effluents of a Peruvian Tannery

The effluents generated by the tannery industry have a high content of chromium and other toxic elements, representing a potential threat to ecosystems. An eco-friendly alternative to treat these effluents is the use of microorganisms, such as fungi, with the capacity to biosorb heavy metals. The present work aims to determine the effect of the molasses concentration, pH variation, and time on the removal of total chromium using the filamentous fungus Trichoderma spp. An experimental design was adopted using pH (4 and 6), concentrations of molasses (0.5 and 1%), and time (8 and 12 days) as independent variables. The Trichoderma inoculum was constant in all the treatments. The different treatments were evaluated after 0, 8, and 12 days by taking 50 mL of sample from each bioreactor. The chromium concentration was subsequently determined in each sample. The results show that treatment 3 (1% molasses and pH 4) showed higher chromium removal after both 8 and 12 days. The concentrations of total chromium decreased from 665 mg/mL to values of 568 mg/mL by day 8 and 486 mg/mL by day 12. These values are, however, still above the maximum threshold imposed by Peruvian law regarding the discharge of non-domestic effluents into the sewage system. The results show that Trichoderma spp. can increasingly remove chromium from the effluent with longer incubation periods. However, future studies are necessary to determine the mechanisms of chromium biosorption by the fungus and the influence of other physicochemical parameters

Genomic investigations of unexplained acute hepatitis in children

Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children

Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19

BackgroundWe previously reported that impaired type I IFN activity, due to inborn errors of TLR3- and TLR7-dependent type I interferon (IFN) immunity or to autoantibodies against type I IFN, account for 15-20% of cases of life-threatening COVID-19 in unvaccinated patients. Therefore, the determinants of life-threatening COVID-19 remain to be identified in similar to 80% of cases.MethodsWe report here a genome-wide rare variant burden association analysis in 3269 unvaccinated patients with life-threatening COVID-19, and 1373 unvaccinated SARS-CoV-2-infected individuals without pneumonia. Among the 928 patients tested for autoantibodies against type I IFN, a quarter (234) were positive and were excluded.ResultsNo gene reached genome-wide significance. Under a recessive model, the most significant gene with at-risk variants was TLR7, with an OR of 27.68 (95%CI 1.5-528.7, P=1.1x10(-4)) for biochemically loss-of-function (bLOF) variants. We replicated the enrichment in rare predicted LOF (pLOF) variants at 13 influenza susceptibility loci involved in TLR3-dependent type I IFN immunity (OR=3.70[95%CI 1.3-8.2], P=2.1x10(-4)). This enrichment was further strengthened by (1) adding the recently reported TYK2 and TLR7 COVID-19 loci, particularly under a recessive model (OR=19.65[95%CI 2.1-2635.4], P=3.4x10(-3)), and (2) considering as pLOF branchpoint variants with potentially strong impacts on splicing among the 15 loci (OR=4.40[9%CI 2.3-8.4], P=7.7x10(-8)). Finally, the patients with pLOF/bLOF variants at these 15 loci were significantly younger (mean age [SD]=43.3 [20.3] years) than the other patients (56.0 [17.3] years; P=1.68x10(-5)).ConclusionsRare variants of TLR3- and TLR7-dependent type I IFN immunity genes can underlie life-threatening COVID-19, particularly with recessive inheritance, in patients under 60 years old

Global economic burden of unmet surgical need for appendicitis

Background There is a substantial gap in provision of adequate surgical care in many low- and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US $92 492 million using approach 1 and$73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was $95 004 million using approach 1 and$75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially

Global economic burden of unmet surgical need for appendicitis

Background There is a substantial gap in provision of adequate surgical care in many low- and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US $92 492 million using approach 1 and$73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was $95 004 million using approach 1 and$75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially