Effect of enhanced pCO2 levels on the production of dissolved organic carbon and transparent exopolymer particles in short-term bioassay experiments

It has been proposed that increasing levels of pCO(2) in the surface ocean will lead to more partitioning of the organic carbon fixed by marine primary production into the dissolved rather than the particulate fraction. This process may result in enhanced accumulation of dissolved organic carbon (DOC) in the surface ocean and/or concurrent accumulation of transparent exopolymer particles (TEPs), with important implications for the functioning of the marine carbon cycle. We investigated this in shipboard bioassay experiments that considered the effect of four different pCO(2) scenarios (ambient, 550, 750 and 1000 mu atm) on unamended natural phytoplankton communities from a range of locations in the northwest European shelf seas. The environmental settings, in terms of nutrient availability, phytoplankton community structure and growth conditions, varied considerably between locations. We did not observe any strong or consistent effect of pCO(2) on DOC production. There was a significant but highly variable effect of pCO(2) on the production of TEPs. In three of the five experiments, variation of TEP production between pCO(2) treatments was caused by the effect of pCO(2) on phytoplankton growth rather than a direct effect on TEP production. In one of the five experiments, there was evidence of enhanced TEP production at high pCO(2) (twice as much production over the 96 h incubation period in the 750 mu atm treatment compared with the ambient treatment) independent of indirect effects, as hypothesised by previous studies. Our results suggest that the environmental setting of experiments (community structure, nutrient availability and occurrence of phytoplankton growth) is a key factor determining the TEP response to pCO(2) perturbations

Contrasting effects of temperature and winter mixing on the seasonal and inter-annual variability of the carbonate system in the Northeast Atlantic Ocean

Future climate change as a result of increasing atmospheric CO2 concentrations is expected to strongly affect the oceans, with shallower winter mixing and consequent reduction in primary production and oceanic carbon drawdown in low and mid-latitudinal oceanic regions. Here we test this hypothesis by examining the effects of cold and warm winters on the carbonate system in the surface waters of the Northeast Atlantic Ocean for the period between 2005 and 2007. Monthly observations were made between the English Channel and the Bay of Biscay using a ship of opportunity program. During the colder winter of 2005/2006, the maximum depth of the mixed layer reached up to 650 m in the Bay of Biscay, whilst during the warmer (by 2.6 a± 0.5 a°C) winter of 2006/2007 the mixed layer depth reached only 300 m. The inter-annual differences in late winter concentrations of nitrate (2.8 ± 1.1 μmol l−1) and dissolved inorganic carbon (22 a± 6 μmol kg−1, with higher concentrations at the end of the colder winter (2005/2006), led to differences in the dissolved oxygen anomaly and the chlorophyll <i>α</i>-fluorescence data for the subsequent growing season. In contrast to model predictions, the calculated air-sea CO2 fluxes (ranging from +3.7 to ĝ̂'4.8 mmol mĝ̂'2 d−1) showed an increased oceanic CO2 uptake in the Bay of Biscay following the warmer winter of 2006/2007 associated with wind speed and sea surface temperature differences. ©Author(s) 2010. CC Attribution 3.0 License

Phytoplankton responses and associated carbon cycling during shipboard carbonate chemistry manipulation experiments conducted around Northwest European shelf seas

The ongoing oceanic uptake of anthropogenic carbon dioxide (CO2) is significantly altering the carbonate chemistry of seawater, a phenomenon referred to as ocean acidification. Experimental manipulations have been increasingly used to gauge how continued ocean acidification will potentially impact marine ecosystems and their associated biogeochemical cycles in the future; however, results amongst studies, particularly when performed on natural communities, are highly variable, which in part likely reflects inconsistencies in experimental approach. To investigate the potential for identification of more generic responses and greater experimentally reproducibility, we devised and implemented a series of highly replicated (n = 8), short term (2–4 days) multi-level (≥ 4 conditions) carbonate chemistry/nutrient manipulation experiments on a range of natural microbial communities sampled in Northwest European shelf seas. Carbonate chemistry manipulations and resulting biological responses were found to be highly reproducible within individual experiments and to a lesser extent between geographically different experiments. Statistically robust reproducible physiological responses of phytoplankton to increasing pCO2, characterized by a suppression of net growth for small sized cells (< 10 µm), were observed in the majority of the experiments, irrespective of nutrient status. Remaining between-experiment variability was potentially linked to initial community structure and/or other site-specific environmental factors. Analysis of carbon cycling within the experiments revealed the expected increased sensitivity of carbonate chemistry to biological processes at higher pCO2 and hence lower buffer capacity. The results thus emphasize how biological-chemical feedbacks may be altered in the future ocean

Evidence of nitrification associated with globally distributed pelagic jellyfish

Often considered detrimental to the environment and human activities, jellyfish blooms are increasing in several coastal regions worldwide. Yet, the overall effect of these outbreaks on ecosystem productivity and structure are not fully understood. Here we provide evidence for a so far unanticipated role of jellyfish in marine nitrogen cycling. Pelagic jellyfish release nitrogen as a metabolic waste product in form of ammonium. Yet, we observed high rates of nitrification (NH4+ → NO3−, 5.7–40.8 nM gWW−1 [wet weight] h−1) associated with the scyphomedusae Aurelia aurita, Chrysaora hysoscella, and Chrysaora pacifica and low rates of incomplete nitrification (NH4+ → NO2−, 1.0–2.8 nM gWW−1 h−1) associated with Chrysaora fulgida, C. hysoscella, and C. pacifica. These observations indicate that microbes living in association with these jellyfish thrive by oxidizing the readily available ammonia to nitrite and nitrate. The four studied species have a large geographic distribution and exhibit frequent population outbreaks. We show that, during such outbreaks, jellyfish‐associated release of nitrogen can provide more than 100% of the nitrogen required for primary production. These findings reveal a so far overlooked pathway when assessing pelagic nitrification rates that might be of particular relevance in nitrogen depleted surface waters and at high jellyfish population densities

Seasonal and inter-annual variability in nutrient supply in relation to mixing in the Bay of Biscay

Post-print

Operating manual for the Marianda (Versatile INstrument for the Determination of Titration Alkalinity) VINDTA 3C for the laboratory based determination of Total Alkalinity and Total Dissolved Inorganic Carbon in seawater

All chemical and biogeochemical process in the sea are affected by the acidity of the water. Acidity is therefore fundamental property of seawater. The growing concern that the acidity of the oceans might be increasing has revealed weaknesses in our knowledge of this fundamental property and its variation in space and time. In 2008 the DEFRApH project (DEFRA contract ME4133) was initiated to provide this missing information in UK related waters. It required sampling for and analysis of the total inorganic carbon (DIC) and total alkalinity (TA) content of samples. This report documents the procedures used for operation of the VINDTA 3C analysis instrument (manufactured by Ludger Mintrop, Marianda, Kiel, Germany) developed at NOC since 2005. It covers instrument set up, daily procedures and the calculation of the final reported concentrations

Coccolithophores on the North-West European Shelf: calcification rates and environmental controls

Coccolithophores are a key functional group in terms of the pelagic production of calcium carbonate (calcite), although their contribution to shelf sea biogeochemistry, and how this relates to environmental conditions, is poorly constrained. Measurements of calcite production (CP) and coccolithophore abundance were made on the north-west European shelf to examine trends in coccolithophore calcification along natural gradients of carbonate chemistry, macronutrient availability and plankton composition. Similar measurements were also made in three bioassay experiments where nutrient (nitrate, phosphate) and pCO2 levels were manipulated. Nanoflagellates (< 10 μm) dominated chlorophyll biomass and primary production (PP) at all but one sampling site, with CP ranging from 0.6 to 9.6 mmol C m−2 d−1. High CP and coccolithophore abundance occurred in a diatom bloom in fully mixed waters off Heligoland, but not in two distinct coccolithophore blooms in the central North Sea and Western English Channel. Coccolithophore abundance and CP showed no correlation with nutrient concentrations or ratios, while significant (p < 0.01) correlations between CP, cell-specific calcification (cell-CF) and irradiance in the water column highlighted how light availability exerts a strong control on pelagic CP. In the experimental bioassays, Emiliania-huxleyi-dominated coccolithophore communities in shelf waters (northern North Sea, Norwegian Trench) showed a strong response in terms of CP to combined nitrate and phosphate addition, mediated by changes in cell-CF and growth rates. In contrast, an offshore diverse coccolithophore community (Bay of Biscay) showed no response to nutrient addition, while light availability or mortality may have been more important in controlling this community. Sharp decreases in pH and a rough halving of calcite saturation states in the bioassay experiments led to decreased CP in the Bay of Biscay and northern North Sea, but not the Norwegian Trench. These decreases in CP were related to slowed growth rates in the bioassays at elevated pCO2 (750 μatm) relative to those in the ambient treatments. The combined results from our study highlight the variable coccolithophore responses to irradiance, nutrients and carbonate chemistry in north-west European shelf waters, which are mediated by changes in growth rates, cell-CF and species composition

Iron and silicic acid concentrations together regulate Si uptake in the equatorial Pacific Ocean

The effects of added Si and Fe on the rate of silicic acid uptake were examined during two cruises to the equatorial Pacific upwelling zone between 110ºW and 140ºW. Maximum uptake rates of Si (Vmax) were highly consistent with a mean of 0.026 ± 0.007 h-1(n = 29), implying maximum diatom growth rates of ~0.6 d-1. Half-saturation constants for Si uptake (KS) also showed little variance, averaging 1.7 ± 0.7 mmol L-1 Si(OH)4. No ecologically significant spatial or temporal patterns for either Vmax or KS were observed. Comparison of Si uptake rates at the ambient silicic acid concentration (Vamb) with Vmax indicated that the ambient [Si(OH)4] restricted Vamb to an average of 63% ± 13% of Vmax. Fe additions also caused significant increases in both Vmax and Vamb, indicating that the rate of Si uptake was also regulated by the ambient [Fe]. Fe additions had a variable effect on KS, but they consistently increased both Vmax and the initial slope of the kinetic curve (Vmax :KS), and thus the diatom assemblages’ ability to take up Si(OH)4 at low concentrations. Added Fe or Si increased Si uptake rates by 87% ± 59% and 69% ± 31%, respectively, indicating nearly equal roles for the two elements in limiting rates of Si uptake in situ. The largest average increase in Si uptake rates, 172% ± 43%, was observed when both Si and Fe were added, implying that together Si and Fe restricted Si uptake rates by almost a factor of three. <br/

Carbon cycling and phytoplankton responses within highly-replicated shipboard carbonate chemistry manipulation experiments conducted around Northwest European Shelf Seas

Abstract. The ongoing oceanic uptake of anthropogenic carbon dioxide (CO2) is significantly altering the carbonate chemistry of seawater, a phenomenon referred to as ocean acidification. Experimental manipulations have been increasingly used to gauge how continued ocean acidification will potentially impact marine ecosystems and their associated biogeochemical cycles in the future; however, results amongst studies, particularly when performed on natural communities, are highly variable, which in part likely reflects inconsistencies in experimental approach. To investigate the potential for identification of more generic responses and greater experimentally reproducibility, we devised and implemented a series of highly replicated (n = 8), short term (2–4 days) multi-level (&amp;amp;geq; 4 conditions) carbonate chemistry/nutrient manipulation experiments on a range of natural microbial communities sampled in Northwest European shelf seas. Carbonate chemistry manipulations and resulting biological responses were found to be highly reproducible within individual experiments and to a lesser extent between geographically different experiments. Statistically robust reproducible physiological responses of phytoplankton to increasing pCO2, characterized by a suppression of net growth for small sized cells (&lt; 10 μm), were observed in the majority of the experiments, irrespective of nutrient status. Remaining between-experiment variability was potentially linked to initial community structure and/or other site-specific environmental factors. Analysis of carbon cycling within the experiments revealed the expected increased sensitivity of carbonate chemistry to biological processes at higher pCO2 and hence lower buffer capacity. The results thus emphasize how biological-chemical feedbacks may be altered in the future ocean. </jats:p