Chasing snails:Automating the processing of EMCCD images of luminescence from opercula

Opercula of the gastropod Bithynia tentaculata are composed of calcite, and are typically 2–4 mm in length. They emit a thermoluminescence (TL) signal that can be used for dose reconstruction, and spatially resolved TL data from them can be obtained using an electron multiplying charge coupled device (EMCCD). However, when multiple measurements are made of the same sample with imaging detectors such as the EMCCD, registering the different images is crucial so that when regions of interest (ROI) are defined they consistently relate to the same part of the specimen. Previous work on opercula has undertaken this registration by hand, but this is prohibitively time consuming and is also potentially prone to human error. An automated registration process is described, and its use is illustrated using a dose recovery experiment. Without registration more than half of the regions of interest defined across the operculum failed the recycling test, and for those ROIs which did pass recycling the dose recovery ratio varied from 0.7 to 1.2. After registration more than 97% of ROIs passed recycling and all these ROIs gave dose recovery ratios within two sigma of unity. The automated registration process described here has potential for application to other types of solid sample such as rock slices provided they are not perfectly circular.</p

Empirical assessment of beta dose heterogeneity in sediments:Implications for luminescence dating

Optically stimulated luminescence (OSL) dating of single grains is often required to determine an accurate age for partially-bleached sediment by identifying those grains with OSL signals that were well bleached prior to burial. However, single-grain De distributions are typically characterised by greater amounts of scatter in comparison to multiple grains. Here we investigate the scatter in single-grain De distributions of quartz from 56 proglacial samples associated with the retreat of the last British-Irish Ice Sheet. Our findings provide the first empirical dataset showing that beta-dose heterogeneity can impact the extrinsic scatter in single-grain De distributions, in addition to partial bleaching in nature. The additional scatter in single-grain De distributions caused by beta-dose heterogeneity suggests that it is inappropriate to apply a fixed threshold to determine between well-bleached and partially-bleached De distributions, but the skewness of the De distributions could alternatively be used. Autoradiography and QEMSCAN analyses show that there was a negative relationship between the relative standard deviation (RSD) of the beta-dose heterogeneity and the beta dose-rate. This relationship offers the opportunity to infer the RSD of the beta-dose heterogeneity for each sample using just the beta dose-rate, instead of acquiring empirical data for every sample. For this large suite of sedimentary samples, we observe a minimum OD of 20 % arising from the effects of beta-dose heterogeneity (Fig. 3e), which should be added (in quadrature) to the intrinsic OD to determine σb for the minimum age model (MAM) to calculate accurate OSL ages and prevent underestimation of the burial age

A comparison of multiple luminescence chronometers at Voordrag, South Africa

A suite of 10 samples collected from an 11 m thick colluvial sequence at Voordrag, KwaZulu-Natal, South Africa, have been used to undertake a comparison of different luminescence methods. Good agreement is found between single grain quartz optically stimulated luminescence (OSL) and single grain K-feldspar post-infrared infrared-stimulated luminescence (post-IR IRSL) ages, with the exception of the basal samples where the quartz OSL signal is saturated. Multiple grain quartz OSL consistently yields ages older than single grain OSL methods. Multiple grain feldspar ages derived from the IRSL50 signal are underestimated due to anomalous fading. A previously published radiocarbon chronology yields ages that are younger than those from single grain quartz OSL and post-IR IRSL, and this is most likely due to contamination with younger carbon. Identifying the effect of saturation on the quartz OSL signal remains challenging when quartz is dated in isolation. However, using a paired quartz/feldspar dating approach is an effective way of identifying the impact of saturation on the OSL signal

Exploring sources of variation in thermoluminescence emissions and anomalous fading in alkali feldspars

Alkali feldspar is routinely used in retrospective dosimetry using luminescence methods. However there is a signal loss over time, termed ‘anomalous fading’, which results in age underestimation if uncorrected. Although significant improvements have been made in recent years, luminescence dating of feldspars remains challenging. This paper investigates the relationships between chemistry, structural state and the scale of exsolution with thermoluminescence (TL) emission spectra and infrared stimulated luminescence (IRSL) fading rates. We measure TL emission spectra, where possible linking the recombination site to physical features of the feldspar crystals. We show that fading rates are lowest in ordered end-member Na- and K-feldspars but significantly greater in disordered end-members, showing that Al–Si order influences fading. As well as having very low fading rates, ordered end-member samples have distinctive TL emission spectra, with the yellow-green emission dominant, while all other samples have a dominant blue emission. Perthite, i.e. exsolved members of the (Na,K)-feldspar solid solution, show greater fading than disordered end-members and fading is greatest in semi-coherent macroperthite. We propose that the state of Al–Si-order, and the occurrence of defects and dislocations at the perthite lamellar interfaces influence anomalous fading rates in feldspar