Pharmaceutical and personal care products-induced stress symptoms and detoxification mechanisms in cucumber plants.

Contamination of agricultural soils by pharmaceutical and personal care products (PPCPs) resulting from the application of treated wastewater, biosolids and animal wastes constitutes a potential environmental risk in many countries. To date a handful of studies have considered the phytotoxicity of individual PPCPs in crop plants, however, little is known about the effect of PPCPs as mixtures at environmentally relevant levels. This study investigated the uptake and transport, physiological responses and detoxification of a mixture of 17 PPCPs in cucumber seedlings. All PPCPs were detected at higher concentrations in roots compared to leaves, with root activity inhibited in a dose-dependent manner. At 5-50 μg/L, the mature leaves exhibited burnt edges as well as a reduction in photosynthesis pigments. Reactive oxygen species (ROS) production and lipid peroxidation increased with increasing PPCP concentrations; and their contents were greater in roots than in leaves for all PPCP treatments. Enzymes involved in various functions, including oxidative stress (superoxide dismutase and ascorbate peroxidase) and xenobiotic metabolism (peroxidase and glutathione S-transferase), were elevated to different levels depending on the PPCP concentration. Glutathione content gradually increased in leaves, while a maxima occurred at 0.5 μg L-1 PPCPs in roots, followed by a decrease thereafter. This study illustrated the complexity of phytotoxicity after exposure to PPCP mixtures, and provided insights into the molecular mechanisms likely responsible for the detoxification of PPCPs in higher plants

A Story of the Washington County Unorganized Territories

The story of the past of any place or people is a history, but this story is so brief and incomplete, I gave the title of “A Story”. Another person could have written quite a different story based on other facts. This story is based on facts collected from various sources and arranged in three ways. Scattered through one will find pictures, mostly old and mostly found in the Alexander-Crawford Historical Society files or with my families’ files. Following this introduction is a series on pictures taken by my great-grandfather, John McAdam Murchie. Next we have a text describing the past by subject. Those subjects are listed at the beginning of that section. The third section is a story told by place. The story of each of the places (32 townships, 3 plantations and a couple of organized towns) is told briefly, but separately. These stories are mostly in phrases and in chronological order. The listed landowners are very incomplete and meant only to give names to the larger picture of ownership from 1783. Maps supplement the stories

Modelling the spring ozone maximum and the interhemispheric asymmetry in the remote marine boundary layer 1. Comparison with surface and ozonesonde measurements

Here we report a modelling study of the spring ozone maximum and its interhemispheric asymmetry in the remote marine boundary layer (MBL). The modelled results are examined at the surface and on a series of time-height cross sections at several locations spread over the Atlantic, the Indian, and the Pacific Oceans. Comparison of model with surface measurements at remote MBL stations indicate a close agreement. The most striking feature of the hemispheric spring ozone maximum in the MBL can be most easily identified at the NH sites of Westman Island, Bermuda, and Mauna Loa, and at the SH site of Samoa. Modelled ozone vertical distributions in the troposphere are compared with ozone profiles. For the Atlantic and the Indian sites, the model generally produces a hemispheric spring ozone maximum close to those of the measurements. The model also produces a spring ozone maximum in the northeastern and tropical north Pacific close to those measurements, and at sites in the NH high latitudes. The good agreement between model and measurements indicate that the model can reproduce the proposed mechanisms responsible for producing the spring ozone maximum in these regions of the MBL, lending confidence in the use of the model to investigate MBL ozone chemistry (see part 2 and part 3). The spring ozone maximum in the tropical central south Pacific and eastern equatorial Pacific are less well reproduced by the model, indicating that both the transport of $O_3$ precursors from biomass burning emissions taking place in southeastern Asia, Australia, Oceania, southern Africa, and South America are not well represented in the model in these regions. Overall, the model produces a better simulation at sites where the stratosphere and biomass burning emissions are the major contributors.Comment: 24 pages, 8 figure

A Review of Prosthetic Interface Stress Investigations

Over the last decade, numerous experimental and numerical analyses have been conducted to investigate the stress distribution between the residual limb and prosthetic socket of persons with lower limb amputation. The objectives of these analyses have been to improve our understanding of the residual limb/prosthetic socket system, to evaluate the influence of prosthetic design parameters and alignment variations on the interface stress distribution, and to evaluate prosthetic fit. The purpose of this paper is to summarize these experimental investigations and identify associated limitations. In addition, this paper presents an overview of various computer models used to investigate the residual limb interface, and discusses the differences and potential ramifications of the various modeling formulations. Finally, the potential and future applications of these experimental and numerical analyses in prosthetic design are presented

Characterization of 1.55-μm pulses from a self-seeded gain-switched Fabry-Pérot laser diode using frequency-resolved optical gating

The intensity and frequency chirp of picosecond pulses from a self-seeded gain-switched Fabry-Perot laser diode have been directly measured using the technique of frequency-resolved optical gating. Measurements over an output sidemode suppression ratio (SMSR) range of 15-35 dB show that higher SMSR's are associated with an increasingly linear frequency chirp across the output pulses. This complete pulse characterization allows the conditions for optimum pulse compression to be determined accurately, and indicates that transform-limited, pedestal free pulses can be obtained at an SMSR of 35 dB

Improved abutment dosimetry in segmented-field electron conformal therapy

Purpose: Segmented-field electron conformal therapy is characterized by dose heterogeneity due to unmatched penumbra of abutted fields of differing energy. The present work investigates the potential to decrease dose heterogeneity by approximately matching beam penumbra using energy-specific source-to-collimator distances (SCDs). It was hypothesized that a clinically practical, variable-SCD method that utilizes Cerrobend® custom inserts can deliver segmented-field electron conformal therapy in the energy range of 6-20 MeV with less than ±5% variation in dose spread in the abutment regions of hypothetical planning target volumes (PTVs), i.e. constrain the PTV dose to 85%-105%. Methods: A Varian 15x15-cm2 electron applicator was modified to allow energy-dependent SCDs resulting in energy-dependent air gaps. Air gaps were chosen based on theoretical calculations to approximately match penumbra for 6, 9, 12, 16, and 20 MeV beams at a depth of 1.5 cm in water. Treatment plans developed for four simulated PTVs and a single patient using the variable-SCD applicator were compared to identical plans using the current constant-SCD applicator. Dose plans for the simulated PTVs using the variable-SCD applicator with electron inserts cut with diverging edges were delivered to film in a polystyrene phantom to assess feasibility. Results: Treatment planning results in the four simulated PTVs showed that dose homogeneity in agreement with the hypothesis can be achieved using the variable-SCD applicator. Minimum dose was increased by an average of 4%, and maximum dose was decreased by an average of 4%. On average, the standard deviation of the dose decreased by 29%, and D90-10 decreased by 32%. Measured dose in the abutment regions for all four simulated targets using the modified applicator agreed well with TPS predicted dose. For the patient PTV, the variable-SCD applicator plan predicted a 14% increase in minimum dose, a 10% decrease in maximum dose, and a 22% reduction in both the standard deviation of the dose distribution and D90-10 as compared to the standard applicator plan. Conclusion: The results of this study demonstrated that dose homogeneity in segmented-field electron conformal therapy can be substantially improved by using energy-dependent SCDs to match beam penumbra

Acetaminophen detoxification in cucumber plants via induction of glutathione S-transferases.

Many pharmaceutical and personal care products (PPCPs) enter agroecosystems during reuse of treated wastewater and biosolids, presenting potential impacts on plant development. Here, acetaminophen, one of the most-used pharmaceuticals, was used to explore roles of glutathione (GSH) conjugation in its biotransformation in crop plants. Acetaminophen was taken up by plants, and conjugated quickly with GSH. After exposure to 5 mg L-1 acetaminophen for 144 h, GSH-acetaminophen conjugates were 15.2 ± 1.3 nmol g-1 and 1.2 ± 0.1 nmol g-1 in cucumber roots and leaves, respectively. Glutathione-acetaminophen was also observed in common bean, alfalfa, tomato, and wheat. Inhibition of cytochrome P450 decreased GSH conjugation. Moreover, the GSH conjugate was found to further convert to cysteine and N-acetylcysteine conjugates. Glutathione S-transferase activity was significantly elevated after exposure to acetaminophen, while levels of GSH decreased by 55.4% in roots after 48 h, followed by a gradual recovery thereafter. Enzymes involved in GSH synthesis, regeneration and transport were consistently induced to maintain the GSH homeostasis. Therefore, GST-mediated conjugation likely played a crucial role in minimizing phytotoxicity of acetaminophen and other PPCPs in plants