Herostrasse Office Building

The way the façade appears to be carved out referecnes thenatural material quality of stonehttps://openscholarship.wustl.edu/bcs/1204/thumbnail.jp

Transcriptional changes in powdery mildew infected wheat and Arabidopsis leaves undergoing syringolin-triggered hypersensitive cell death at infection sites

Blumeria graminis f.sp. tritici, the causal agent of powdery mildew in wheat, is an obligate biotrophic fungus that exclusively invades epidermal cells. As previously shown, spraying of a solution of syringolin A, a circular peptide derivative secreted by the phytopathogenic bacterium Pseudomonas syringae pv. syringae, triggers hypersensitive cell death at infection sites in powdery mildew infected wheat. Thus, the fungus is essentially eradicated. Here we show that syringolin A also triggers hypersensitive cell death in Arabidopsis infected with the powdery mildew fungus Erysiphe cichoracearum. To monitor transcriptional changes associated with this effect, we cloned 307 cDNA clones representing 158 unigenes from powdery mildew infected, syringolin A sprayed wheat leaves by a suppression subtractive hybridization cloning procedure. These cDNAs were microarrayed onto glass slides together with 1088 cDNA-AFLP clones from powdery mildew-infected wheat. Microarray hybridization experiments were performed with probes derived from leaves, epidermal tissue, and mesophyll preparations of mildewed or uninfected wheat plants after syringolin A or control treatment. Similar experiments were performed in Arabidopsis using the Affymetrix ATH1 whole genome GeneChip. The results indicate a conserved mode of action of syringolin A as similar gene groups are induced in both species. Prominent groups include genes associated with the proteasomal degradation pathway, mitochondrial and other heat shock genes, genes involved in mitochondrial alternative electron pathways, and genes encoding glycolytic and fermentative enzymes. Surprisingly, in both species the observed transcriptional response to syringolin A was considerably weaker in infected plants as compared to uninfected plants. The results lead to the working hypothesis that cell death observed at infection sites may result from a parasite-induced suppression of the transcriptional response and thus to insufficient production of protective proteins necessary for the recovery of these cells from whatever insult is imposed by syringolin

Analysis of epidermis- and mesophyll-specific transcript accumulation in powdery mildew-inoculated wheat leaves

Powdery mildew is an important disease of wheat caused by the obligate biotrophic fungus Blumeria graminis f. sp. tritici. This pathogen invades exclusively epidermal cells after penetrating directly through the cell wall. Because powdery mildew colonizes exclusively epidermal cells, it is of importance not only to identify genes which are activated, but also to monitor tissue specificity of gene activation. Acquired resistance of wheat to powdery mildew can be induced by a previous inoculation with the non-host pathogen B. graminis f. sp. hordei, the causal agent of barley powdery mildew. The establishment of the resistant state is accompanied by the activation of genes. Here we report the tissue-specific cDNA-AFLP analysis and cloning of transcripts accumulating 6 and 24 h after the resistance-inducing inoculation with B. graminis f. sp. hordei. A total of 25 000 fragments estimated to represent about 17 000 transcripts were displayed. Out of these, 141 transcripts, were found to accumulate after Bgh inoculation using microarray hybridization analysis. Forty-four accumulated predominantly in the epidermis whereas 76 transcripts accumulated mostly in mesophyll tissu

Régime pauvre en hydrates de carbone et douleurs articulaires: quel rapport? [Low-carb diet and joint pain: how are they related?]

We report the case of a 69-year-old male who presented with acute bilateral ankle pain treated as acute gouty arthritis but resistant to colchicine and NSAIDs. In view of very high inflammatory parameters, septic arthritis was initially suspected but ruled out by joint aspiration; the final diagnosis was gouty arthritis. He finally responded well to a short course of oral prednisone. We found this case interesting in view of the initial differential diagnosis and association with a low-carb diet, known to be associated with many metabolic complications among which gout is frequent. [Authors]]]> Diet, Carbohydrate-Restricted ; Arthritis, Gouty fre oai:serval.unil.ch:BIB_F31B6FE6921F 2022-05-07T01:30:01Z openaire documents urnserval <oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:xs="http://www.w3.org/2001/XMLSchema" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"> https://serval.unil.ch/notice/serval:BIB_F31B6FE6921F The 1925 Opium Purchasing Campaign in Laos: The Anatomy of a Colonial Scandal Rapin, A.-J. info:eu-repo/semantics/other misc 2014 <![CDATA[Opium purchasing campaigns, initiated in 1915, were abruptly interrupted ten years later following the discovery that the Douanes &amp; Régies of the General Government of Indochina had been victim of a gigantic fraud. Various inquiries expedited with a view to establishing responsibility for the fiasco, involving purchases made at Luang Prabang in 1925, highlight the dysfunctions affecting the colonial civil service. The 1925 scandal revealed not only the difficulties encountered by the French in their attempts to control the drug trade, but also the prevailing circumstantial inefficiency of the colonial bureaucracy

Vorkommen und Verbreitung der Hummeln in OWL und einigen angrenzenden Gebieten in Nordhessen und Südniedersachsen

Im und über den Raum Ostwestfalen sind bereits zahlreiche entomofaunistische Abhandlungen über verschiedene Gruppen (bes. Lepidoptera - Schmetterlinge, Orthoptera - Geradflügler, Odonata - Libellen, Coleoptera - Käfer etc.) publiziert worden. Umfangreiche faunistische Beiträge über Hautflügler finden sich im wesentlichen nur in den "Mitteilungen" der Arbeitsgemeinschaft ostwestfälisch-lippischer Entomologen e. V., wie z. B. in der Reihe "Insektenfauna und Ökologie der Binnendünen in der südlichen Senne", z. B. KUHLMANN et al. 1990 u. 1991. Näheres über das Vorkommen und die Verbreitung der Hummeln in den ostwestfälischen Naturräumen lässt sich dagegen kaum finden. Im Rahmen dieser Arbeit sollen nun die bisher bekannt gewordene aktuelle Bestandssituation und Verbreitung dieser Großbienen im Bereich des Regierungsbezirkes Detmold aufgezeigt werden. Behandelt werden sowohl die staatenbildenden sogenannten "Echten Hummeln" der Gattung Bombus und die Schmarotzer- oder Kuckuckshummeln (neuerdings ebenfalls in die Gattung Bombus gestellt; vorher als eigene Gattung Psithyrus geführt). Diese gründen keine eigenen Staaten sondern leben sozialparasitisch in Nestern der "Echten Hummeln". Im Gegensatz zu den Bombus-Arten mit den drei Kasten (Weibchen, Männchen und Arbeiterin) treten bei den Schmarotzerhummeln (vormals Gatt. Psithyrus - s. o.) nur Geschlechtstiere (Weibchen und Männchen) auf

CPPD crystal deposition disease in patients with rheumatoid arthritis

The aim of this study was to assess the frequency and the outcome of patients suffering from rheumatoid arthritis in which calcium pyrophosphate dihydrate (CPPD) crystal deposits were found to coexist in synovial fluid analysis. Such association was more frequent than previously believed with CPPD crystals found in 25.8% of 93 patients with rheumatoid arthritis. As a group, a trend toward a worse outcome was suggested by more frequent prostheses of the lower lim

Electron-Microscopy Investigations of the Warping Effect in Pigmented High Density Polyethylene

The warping effect of high-density polyethylene (HDPE) articles produced by organic pigmentation has been studied by scanning electron microscopy. A warping pigment and its warp-free analogue obtained by surface treatment have been observed and compared to an inorganic warp-free pigment. The results indicate that the generally accepted nucleation theory is not sufficient to describe fully the warping effect. An attempt is made to describe this effect in terms of pigment-polymer compatibility

Characterization of the rice pathogen-related protein Rir1a and regulation of the corresponding gene

In rice (Oryza sativa L.), local acquired resistance against Pyricularia oryzae (Cav.), the causal agent of rice blast, can be induced by a preinoculation with the non-host pathogen Pseudomonas syringae pv. syringae. We have cloned a cDNA (Rir1a) and a closely related gene (Rir1b) corresponding to transcripts that accumulate in leaf tissue upon inoculation with P. syringae pv. syringae. The cDNA encodes a putative 107 amino acid protein, Rir1a, that exhibits a putative signal peptide cleavage site in its hydrophobic N-terminal part and a C-terminal part that is relatively rich in glycine and proline. The Rir1b gene contains a Tourist and a Wanderer miniature transposable element in its single intron and encodes a nearly identical protein. Rir1a is similar in sequence (ca. 35% identical and ca. 60% conservatively changed amino acids) to the putative Wir1 family of proteins that are encoded by pathogen-induced transcripts in wheat. Using antibodies raised against a Rir1a-fusion protein we show that Rir1a is secreted from rice protoplasts transiently expressing a 35S::Rir1a construct and that the protein accumulates in the cell wall compartment of rice leaves upon inoculation with P. syringae pv. syringae. Possible roles of Rir1a in pathogen defense are discusse