Identification of the Sex Pheromone of a Protected Species, the Spanish Moon Moth Graellsia isabellae

Sex attractant pheromones are highly sensitive and selective tools for detecting and monitoring populations of insects, yet there has been only one reported case of pheromones being used to monitor protected species. Here, we report the identification and synthesis of the sex pheromone of a protected European moth species, Graellsia isabellae (Lepidoptera: Saturniidae), as the single component, (4E,6E,11Z)-hexadecatrienal. In preliminary field trials, lures loaded with this compound attracted male moths from populations of this species at a number of widely separated field sites in France, Switzerland, and Spain, clearly demonstrating the utility of pheromones in sampling potentially endangered insect species

Characterization of Human DNA Polymerase Delta and Its Subassemblies Reconstituted by Expression in the Multibac System

Mammalian DNA polymerase δ (Pol δ), a four-subunit enzyme, plays a crucial and versatile role in DNA replication and DNA repair processes. We have reconstituted human Pol δ complexes in insect cells infected with a single baculovirus into which one or more subunits were assembled. This system allowed for the efficient expression of the tetrameric Pol δ holoenzyme, the p125/p50 core dimer, the core+p68 trimer and the core+p12 trimer, as well as the p125 catalytic subunit. These were isolated in milligram amounts with reproducible purity and specific activities by a highly standardized protocol. We have systematically compared their activities in order to gain insights into the roles of the p12 and p68 subunits, as well as their responses to PCNA. The relative specific activities (apparent kcat) of the Pol δ holoenzyme, core+p68, core+p12 and p125/p50 core were 100, 109, 40, and 29. The corresponding apparent Kd's for PCNA were 7.1, 8.7, 9.3 and 73 nM. Our results support the hypothesis that Pol δ interacts with PCNA through multiple interactions, and that there may be a redundancy in binding interactions that may permit Pol δ to adopt flexible configurations with PCNA. The abilities of the Pol δ complexes to fully extend singly primed M13 DNA were examined. All the subassemblies except the core+p68 were defective in their abilities to completely extend the primer, showing that the p68 subunit has an important function in synthesis of long stretches of DNA in this assay. The core+p68 trimer could be reconstituted by addition of p12

Basement‐cover decoupling during the inversion of a hyperextended basin: Insights from the Eastern Pyrenees

International audienceDeformation processes related to early stages of collisional belts, especially the inversion of rifted systems remain poorly constrained, partly because evidence of these processes is usually obliterated during the subsequent collision. The Pyrenean belt resulting from the inversion of a Cretaceous hyperextended rifted margin associated with a HT/LP metamorphism in the Internal Metamorphic Zone (IMZ), is a good example for studying the early stage of orogenic deformation. This study is focused on the Eastern Pyrenees where the relation between inverted Mesozoic rifted basins and their basement are well-preserved. By using maximum temperatures (Tmax) estimated by the Raman Spectroscopy of Carbonaceous Materials geothermometer and structural data, we describe the spatial distribution of the various tectono-metamorphic units. Tmax recorded in the sedimentary cover exposed to the north and to the south of a Paleozoic basement block (Agly massif), exceed 550°C, while the Paleozoic metasediments and their autochthonous Mesozoic cover show Tmax <350°C. The metamorphic sedimentary cover is affected by ductile deformation, while the basement is only affected by brittle deformation. Post-metamorphism breccias are observed between the basement and the metamorphic Accepted Article This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article a

Preorogenic Folds and Syn-Orogenic Basement Tilts in an Inverted Hyperextended Margin: The Northern Pyrenees Case Study

International audienceThe Chaînons Béarnais (CB, North Pyrenean Zone) resulted from the Cenozoic contractional reactivation of the salt tectonics-bearing, hyperextended margin that initiated at the Europe-Iberia transition during the Early Cretaceous. In this tectonic scenario, assessing the relative contribution of extension and contraction to the present-day structure is crucial to reconstruct the hyperextended margin geometry and to quantify the subsequent shortening. This study undertakes this issue by defining the relationship between folding and two bedding-independent references: peak temperature isotherms and paleomagnetic data. Isotherms were reconstructed from 76 new measurements of Raman spectroscopy on carbonaceous materials (RSCM) and indicate temperatures at the time of peak metamorphism in the CB (110-85 Ma, end of extension). They are shallowly to moderately northwards dipping and cut across most of the folds deforming the Mesozoic units. Paleomagnetic data from 29 sites evidence a widespread remagnetization carried by pyrrhotite that was probably blocked during the early Paleogene (before the onset of continental collision) and postdated folding in the CB. Abnormal inclinations in this remagnetization suggest syn-collision tilts up to 60°to the north in the back limb of the Axial Zone. Based on the presented data set, we propose that the folding of the cover above the evaporitic décollement was almost fully completed by the end of the Cretaceous extension, with~85-100% of the dip of fold limbs being acquired before the remagnetization time. Cenozoic contraction reactivated the extensional faults in the shallow basement as top-to-the-S thrusts, leading to the passive transport and northwards tilting of the folded cover