Stokes-correlometry analysis of biological tissues with polycrystalline structure

Utilizing Stokes-correlometry analysis a new diagnostic approach has been introduced for quantitative assessment of polarization images of histological sections of optically anisotropic biological tissues with different morphological structures and physiological conditions. The developed approach is based on the quantitative assessment of coordinate and phase distributions of the Stokes vector of scattered light. A combined use of statistic, correlation, and fractal analysis is used for resolving variations in optical anisotropy of biological samples. The proposed combined application of the statistical, correlation, and fractal-based evaluates of spatial distributions of `single-point' polarization azimuth, ellipticity, and `two-point' Stokes vector parameters of polarization images of biological tissues histological sections demonstrates a high accuracy (Ac ≥ 90%) in monitoring of optical anisotropy variations within biological tissues

Mapping of polycrystalline films of biological fluids utilizing the Jones-matrix formalism

Utilizing a polarized light approach, we reconstruct the spatial distribution of birefringence and optical activity in polycrystalline films of biological fluids. The Jones-matrix formalism is used for an accessible quantitative description of these types of optical anisotropy. We demonstrate that differentiation of polycrystalline films of biological fluids can be performed based on a statistical analysis of the distribution of rotation angles and phase shifts associated with the optical activity and birefringence, respectively. Finally, practical operational characteristics, such as sensitivity, specificity and accuracy of the Jones-matrix reconstruction of optical anisotropy, were identified with special emphasis on biomedical application, specifically for differentiation of bile films taken from healthy donors and from patients with cholelithiasis

Insights into polycrystalline microstructure of blood films with 3D Mueller matrix imaging approach

This study introduces a novel approach in the realm of liquid biopsies, employing a 3D Mueller-matrix (MM) image reconstruction technique to analyze dehydrated blood smear polycrystalline structures. Our research centers on exploiting the unique optical anisotropy properties of blood proteins, which undergo structural alterations at the quaternary and tertiary levels in the early stages of diseases such as cancer. These alterations manifest as distinct patterns in the polycrystalline microstructure of dried blood droplets, offering a minimally invasive yet highly effective method for early disease detection. We utilized a groundbreaking 3D MM mapping technique, integrated with digital holographic reconstruction, to perform a detailed layer-by-layer analysis of partially depolarizing dry blood smears. This method allows us to extract critical optical anisotropy parameters, enabling the differentiation of blood films from healthy individuals and prostate cancer patients. Our technique uniquely combines polarization-holographic and differential MM methodologies to spatially characterize the 3D polycrystalline structures within blood films. A key advancement in our study is the quantitative evaluation of optical anisotropy maps using statistical moments (first to fourth orders) of linear and circular birefringence and dichroism distributions. This analysis provides a comprehensive characterization of the mean, variance, skewness, and kurtosis of these distributions, crucial for identifying significant differences between healthy and cancerous samples. Our findings demonstrate an exceptional accuracy rate of over 90% for the early diagnosis and staging of cancer, surpassing existing screening methods. This high level of precision and the non-invasive nature of our technique mark a significant advancement in the field of liquid biopsies. It holds immense potential for revolutionizing cancer diagnosis, early detection, patient stratification, and monitoring, thereby greatly enhancing patient care and treatment outcomes. In conclusion, our study contributes a pioneering technique to the liquid biopsy domain, aligning with the ongoing quest for non-invasive, reliable, and efficient diagnostic methods. It opens new avenues for cancer diagnosis and monitoring, representing a substantial leap forward in personalized medicine and oncology

Embossed topographic depolarisation maps of biological tissues with different morphological structures

Layered topographic maps of the depolarisation due to diffuse biological tissues are produced using a polarisation-holographic Mueller matrix method approach. Histological sections of myocardial tissue with a spatially structured optically anisotropic fibrillar network, and parenchymal liver tissue with a polycrystalline island structure are successfully mapped. The topography of the myocardium maps relates to the scattering multiplicity within the volume and the specific morphological structures of the biological crystallite networks. The overall depolarisation map is a convolution of the effects of these two factors. Parenchymal liver tissues behave broadly similarly, but the different biological structures present cause the degree of scattering multiplicity to increase more rapidly with increasing phase. Through statistical analysis, the dependences of the magnitudes of the first to fourth order statistical moments are determined. These moments characterise the changing distributions of the depolarisation values through the volume of biological tissues with different morphological structures. Parenchymal liver tissue depolarisation maps are characterised by larger mean and variance, and less skewness and kurtosis, compared to the distributions for the myocardium. This work demonstrates that a polarisation-holographic Mueller matrix method can be applied to the assessment of the 3D morphology of biological tissues, with applications in disease diagnosis

Mueller-matrix imaging polarimetry elevated by wavelet decomposition and polarization-singular processing for analysis of specific cancerous tissue pathology

Significance Mueller-matrix polarimetry is a powerful method allowing for the visualization of malformations in biological tissues and quantitative evaluation of alterations associated with the progression of various diseases. This approach, in fact, is limited in observation of spatial localization and scale-selective changes in the poly-crystalline compound of tissue samples. Aim We aimed to improve the Mueller-matrix polarimetry approach by implementing the wavelet decomposition accompanied with the polarization-singular processing for express differential diagnosis of local changes in the poly-crystalline structure of tissue samples with various pathology. Approach Mueller-matrix maps obtained experimentally in transmitted mode are processed utilizing a combination of a topological singular polarization approach and scale-selective wavelet analysis for quantitative assessment of the adenoma and carcinoma histological sections of the prostate tissues. Results A relationship between the characteristic values of the Mueller-matrix elements and singular states of linear and circular polarization is established within the framework of the phase anisotropy phenomenological model in terms of linear birefringence. A robust method for expedited (up to ∼15 min) polarimetric-based differential diagnosis of local variations in the poly-crystalline structure of tissue samples containing various pathology abnormalities is introduced. Conclusions The benign and malignant states of the prostate tissue are identified and assessed quantitatively with a superior accuracy provided by the developed Mueller-matrix polarimetry approach

3D Mueller Matrix Reconstruction of the Optical Anisotropy Parameters of Myocardial Histopathology Tissue Samples

Diseases affecting myocardial tissues are currently a leading cause of death in developed nations. Fast and reliable techniques for analysing and understanding how tissues are affected by disease and respond to treatment are fundamental to combating the effects of heart disease. A 3D Mueller matrix method that reconstructs the linear and circular birefringence and dichroism parameters has been developed to image the biological structures in myocardial tissues. The required optical data is gathered using a Stokes polarimeter and then processed mathematically to recover the individual optical anisotropy parameters, expanding on existing 2D Mueller matrix implementations by combining with a digital holography approach. Changes in the different optical anisotropy parameters are rationalised with reference to the general tissue structure, such that the structures can be identified from the anisotropy distributions. The first to fourth order statistical moments characterising the distribution of the parameters of the optical anisotropy of the polycrystalline structure of the partially depolarising layer of tissues in different phase sections of their volumes are investigated and analysed. The third and fourth order statistical moments are found to be the most sensitive to changes in the phase and amplitude anisotropy. The possibility of forensic medical differentiation of death in cases of acute coronary insufficiency (ACI) and coronary heart disease (CHD) is considered as a diagnostic application. The optimal phase plane ( θ ∗ = 0.7 r a d ) has been found, in which excellent differentiation accuracy is achieved ACI and CHD - A c ( Δ Z 4 ( θ ∗ , Φ L , Δ L ) ) = 93.05 % ÷ 95.8 % . A comparative analysis of the accuracy of the Mueller-matrix reconstruction of the parameters of the optical anisotropy of the myocardium in different phase planes ( θ = 0.9 r a d and θ = 1.2 r a d ), as well as the 2D Mueller-matrix reconstruction method was carried out. This work demonstrates that a 3D Mueller matrix method can be used to effectively analyse the optical anisotropy parameters of myocardial tissues with potential for definitive diagnostics in forensic medicine

The Interconnection between the Coordinate Distribution of Mueller-Matrixes Images Characteristic Values of Biological Liquid Crystals Net and the Pathological Changes of Human Tissues

We have theoretically grounded conceptions of characteristic points observed in coordinate distributions of Mueller matrix elements for a network of human tissue biological crystals. The interrelation between polarization singularities of laser images inherent to these biological crystals and characteristic values of above matrix elements is found. We have determined the criteria for statistical diagnostics of pathological changes in the birefringent structure of biological crystal network by using myometrium tissue as an example

Mapping of polycrystalline films of biological fluids utilizing the Jones-matrix formalism

Utilizing a polarized light approach, we reconstruct the spatial distribution of birefringence and optical activity in polycrystalline films of biological fluids. The Jones-matrix formalism is used for an accessible quantitative description of these types of optical anisotropy. We demonstrate that differentiation of polycrystalline films of biological fluids can be performed based on a statistical analysis of the distribution of rotation angles and phase shifts associated with the optical activity and birefringence, respectively. Finally, practical operational characteristics, such as sensitivity, specificity and accuracy of the Jones-matrix reconstruction of optical anisotropy, were identified with special emphasis on biomedical application, specifically for differentiation of bile films taken from healthy donors and from patients with cholelithiasis

From Victim Diaspora to Transborder Citizenship? : Diaspora formation and transnational relations among Kurds in France and Sweden

Denna avhandling är en komparativ undersökning av pågående förändringsprocesser bland kurder i Marseillesregionen i Frankrike och Stockholmsregionen i Sverige. I fokus står skiftet från en endimensionell och offerrelaterad kurdisk diasporisk identitet mot en mer sammansatt och aktiv. Studien går bortom entydiga erfarenheter av smärta, trauma och offerkänsla i syfte att lyfta fram en rad andra diasporiska situationer och företeelser såsom institutionella och transnationella formationer, assabiyya nätverk, ”on air” och ”online” verksamheter, kulturella och litterära aktiviteter osv., som samtliga är centrala element när det gäller att upprätthålla ett gränsöverskridande medborgarskap bland diasporiska kurder i de bägge länderna. Studien vidhåller dessutom att kurder i både Sverige och Frankrike på olika sätt är utsatta för diskriminering och socialt utanförskap. Avhandlingen visar hur kurder i Frankrike och Sverige utvecklar olika diasporiska diskurser och handlingsmönster. Beroende på en relativt likartad social bakgrund och på den exkluderande politiska miljö som finns i Frankrike så upprätthåller kurderna i Marseillesområdet en påtagligt offerrelaterad diasporisk diskurs. Samtidigt är framväxten av ett gränsöverskridande medborgarskap här starkt begränsad. Eftersom kurderna i Sverige är dels jämförelsevis socialt och politiskt diversifierade och dels vistas i en mer gynnsam politisk miljö upprätthåller de inte bara en mer flexibel diasporisk diskurs än kurder i Marseillesområdet. De har också utvecklat ett mer långtgående gränsöverskridande medborgarskap.This study is a comparative exploration of an ongoing process of change from a mono-dimensional, victim-related Kurdish diasporic identity to a more modulated, dynamic and active form of it among Kurds in the Marseille region in France and the Stockholm region in Sweden. The study goes beyond the experience of pain and trauma and the sense of victimhood in order to depict a multitude of other diasporic situations and trajectories such as institutional and transnational arrangements, assabiyya networks, “on air” and cyberspace “online” involvements, cultural and literary activities, and so forth, which are necessary elements for the development of the practice of transborder citizenship among diasporan Kurds in the two countries. The study argues that the Kurds in France and Sweden have conceived different diasporic discourses and at the same time have chosen different modes of action. Due to their uniform social background and the exclusionary French political environment, the Kurds in the region of Marseille maintain a tangible victim diaspora discourse and limited practice of transborder citizenship. As the Kurds in Sweden are socially and politically diversified, and as they live in the more favorable Swedish political environment, they maintain not only a flexible diaspora discourse but also a more highly developed practice of transborder citizenship than the Kurds in the Marseille region

Mapping of polycrystalline films of biological fluids utilizing the Jones-matrix formalism

Abstract Utilizing a polarized light approach, we reconstruct the spatial distribution of birefringence and optical activity in polycrystalline films of biological fluids. The Jones-matrix formalism is used for an accessible quantitative description of these types of optical anisotropy. We demonstrate that differentiation of polycrystalline films of biological fluids can be performed based on a statistical analysis of the distribution of rotation angles and phase shifts associated with the optical activity and birefringence, respectively. Finally, practical operational characteristics, such as sensitivity, specificity and accuracy of the Jones-matrix reconstruction of optical anisotropy, were identified with special emphasis on biomedical application, specifically for differentiation of bile films taken from healthy donors and from patients with cholelithiasis