Double-lambda microscopic model for entangled light generation by four-wave-mixing

Motivated by recent experiments, we study four-wave-mixing in an atomic double-{\Lambda} system driven by a far-detuned pump. Using the Heisenberg-Langevin formalism, and based on the microscopic properties of the medium, we calculate the classical and quantum properties of seed and conju- gate beams beyond the linear amplifier approximation. A continuous variable approach gives us access to relative-intensity noise spectra that can be directly compared to experiments. Restricting ourselves to the cold-atom regime, we predict the generation of quantum-correlated beams with a relative-intensity noise spectrum well below the standard quantum limit (down to -6 dB). Moreover entanglement between seed and conjugate beams measured by an inseparability down to 0.25 is expected. This work opens the way to the generation of entangled beams by four-wave mixing in a cold atomic sample.Comment: 11 pages, 6 figures, submitted to PR

Rubidium-87 Bose-Einstein condensate in an optically plugged quadrupole trap

We describe an experiment to produce 87Rb Bose-Einstein condensates in an optically plugged magnetic quadrupole trap, using a blue-detuned laser. Due to the large detuning of the plug laser with respect to the atomic transition, the evaporation has to be carefully optimized in order to efficiently overcome the Majorana losses. We provide a complete theoretical and experimental study of the trapping potential at low temperatures and show that this simple model describes well our data. In particular we demonstrate methods to reliably measure the trap oscillation frequencies and the bottom frequency, based on periodic excitation of the trapping potential and on radio-frequency spectroscopy, respectively. We show that this hybrid trap can be operated in a well controlled regime that allows a reliable production of degenerate gases.Comment: 13 pages, 8 figure

Photoionisation loading of large Sr+ ion clouds with ultrafast pulses

This paper reports on photoionisation loading based on ultrafast pulses of singly-ionised strontium ions in a linear Paul trap. We take advantage of an autoionising resonance of Sr neutral atoms to form Sr+ by two-photon absorption of femtosecond pulses at a wavelength of 431nm. We compare this technique to electron-bombardment ionisation and observe several advantages of photoionisation. It actually allows the loading of a pure Sr+ ion cloud in a low radio-frequency voltage amplitude regime. In these conditions up to 4x10^4 laser-cooled Sr+ ions were trapped

Nonadhesive Culture System as a Model of Rapid Sphere Formation with Cancer Stem Cell Properties

BACKGROUND: Cancer stem cells (CSCs) play an important role in tumor initiation, progression, and metastasis and are responsible for high therapeutic failure rates. Identification and characterization of CSC are crucial for facilitating the monitoring, therapy, or prevention of cancer. Great efforts have been paid to develop a more effective methodology. Nevertheless, the ideal model for CSC research is still evolving. In this study, we created a nonadhesive culture system to enrich CSCs from human oral squamous cell carcinoma cell lines with sphere formation and to characterize their CSC properties further. METHODS: A nonadhesive culture system was designed to generate spheres from the SAS and OECM-1 cell lines. A subsequent investigation of their CSC properties, including stemness, self-renewal, and chemo- and radioresistance in vitro, as well as tumor initiation capacity in vivo, was also performed. RESULTS: Spheres were formed cost-effectively and time-efficiently within 5 to 7 days. Moreover, we proved that these spheres expressed putative stem cell markers and exhibited chemoradiotherapeutic resistance, in addition to tumor-initiating and self-renewal capabilities. CONCLUSIONS: Using this nonadhesive culture system, we successfully established a rapid and cost-effective model that exhibits the characteristics of CSCs and can be used in cancer research

Cell–cell and cell–matrix dynamics in intraperitoneal cancer metastasis

The peritoneal metastatic route of cancer dissemination is shared by cancers of the ovary and gastrointestinal tract. Once initiated, peritoneal metastasis typically proceeds rapidly in a feed-forward manner. Several factors contribute to this efficient progression. In peritoneal metastasis, cancer cells exfoliate into the peritoneal fluid and spread locally, transported by peritoneal fluid. Inflammatory cytokines released by tumor and immune cells compromise the protective, anti-adhesive mesothelial cell layer that lines the peritoneal cavity, exposing the underlying extracellular matrix to which cancer cells readily attach. The peritoneum is further rendered receptive to metastatic implantation and growth by myofibroblastic cell behaviors also stimulated by inflammatory cytokines. Individual cancer cells suspended in peritoneal fluid can aggregate to form multicellular spheroids. This cellular arrangement imparts resistance to anoikis, apoptosis, and chemotherapeutics. Emerging evidence indicates that compact spheroid formation is preferentially accomplished by cancer cells with high invasive capacity and contractile behaviors. This review focuses on the pathological alterations to the peritoneum and the properties of cancer cells that in combination drive peritoneal metastasis

DMTs and Covid-19 severity in MS: a pooled analysis from Italy and France

We evaluated the effect of DMTs on Covid-19 severity in patients with MS, with a pooled-analysis of two large cohorts from Italy and France. The association of baseline characteristics and DMTs with Covid-19 severity was assessed by multivariate ordinal-logistic models and pooled by a fixed-effect meta-analysis. 1066 patients with MS from Italy and 721 from France were included. In the multivariate model, anti-CD20 therapies were significantly associated (OR = 2.05, 95%CI = 1.39–3.02, p < 0.001) with Covid-19 severity, whereas interferon indicated a decreased risk (OR = 0.42, 95%CI = 0.18–0.99, p = 0.047). This pooled-analysis confirms an increased risk of severe Covid-19 in patients on anti-CD20 therapies and supports the protective role of interferon

Engineering of microfabricated ion traps and integration of advanced on-chip features

Atomic ions trapped in electromagnetic potentials have long been used for fundamental studies in quantum physics. Over the past two decades, trapped ions have been successfully used to implement technologies such as quantum computing, quantum simulation, atomic clocks, mass spectrometers and quantum sensors. Advanced fabrication techniques, taken from other established or emerging disciplines, are used to create new, reliable ion-trap devices aimed at large-scale integration and compatibility with commercial fabrication. This Technical Review covers the fundamentals of ion trapping before discussing the design of ion traps for the aforementioned applications. We overview the current microfabrication techniques and the various considerations behind the choice of materials and processes. Finally, we discuss current efforts to include advanced, on-chip features in next-generation ion traps