Lifestyles and surveillance of sexual and reproductive women’s health

Abstract: Background: The epidemic increase of diseases is closely related to lifestyle changes. The low adhesion to breast self-examination and cervical cytology hinders prevention and early diagnosis during the asymptomatic state of two silent diseases, with nonspecific symptoms that are a major cause of death by cancer in the Portuguese female population. Objectives: To analyze the influence of sociodemographic variables and lifestyles in the surveillance of sexual and reproductive health of Portuguese women. Methods: Quantitative, non-experimental, correlational and cross-sectional study, conducted with a non-probabilistic convenience sample of 522 women aged between 18 and 67 years old, who applied the questionnaires about Breast self-examination, Knowledge about cervical cancer and the Inventory "My Lifestyle" (Ribeiro, 1993. Results: The average age of women is 38.89 years old. Women aged 31-37 years old (28.6%), Portuguese (99.1%), cohabiting with a partner and / or child (74.0%), with an active employment status (67 5%), residing in urban areas (55.8%) with higher education and attending family planning consultations have better lifestyles, without statistical significance (F = 0.016, p = 0.899). Conclusions: Healthy behaviors and lifestyles are crucial to good overall health. Adhesion to surveillance of sexual and reproductive women’s health is influenced by several factors, including the place of residence, employment status and lifestyles. These variables must be considered by health professionals when planning periodic screenings.info:eu-repo/semantics/publishedVersio

Does cultural background influence the dissemination and severity of the COVID-19 pandemic?

The COVID-19 pandemic has spread throughout the globe affecting countries worldwide. However, several differences have been observed in the number of daily new cases, the COVID-19 reproduction rate, and the severity of the disease in different countries. Previous studies have mostly highlighted government restriction policies to mitigate the pandemic effects as reasons for such differences. This study focuses on 101 countries and proposes that each country’s cultural background is also accountable for such differences. We considered the six Hofstede’s cultural dimensions (power distance, individualism, masculinity, uncertainty avoidance, long term orientation, and indulgence) and statistically analyzed their correlation with several COVID-19 impact metrics in comparison to several restriction policies. Our results support our claim that national culture influences both acceptance and subsequent adoption of restriction policies and the implementation by each government of those policies. We highlight that the attitudes towards and trust in political institutions, policies and governance is influenced by the cultural background, which is reflected in the pandemic numbers. As a main takeaway from this study, we conclude that data-driven models which aim at predicting the pandemic impact evolution at a global scale should also include variables that reflect the cultural background of each nation.info:eu-repo/semantics/publishedVersio

Toward the operationalization of Tourism e-Microentrepreneurial Self-Efficacy

With the emergence of websites like People-First Tourism, Airbnb and Vayable, there is a growing number of microentrepreneurs selling accommodation, transportation and experiences directly to prospective customers. These individuals are thought to be different from most entrepreneurs because they are known to be motivated by non-economic drivers such as freedom, passion, or lifestyle, rather than solely by a desire for rapid revenue growth. Accordingly, given that tourism microentrepreneurs have different goals as well as business models relative to typical entrepreneurs, we identified the need to adapt entrepreneurial self-efficacy measures to the specific context of tourism e-microentrepreneurship. Accordingly we developed the Tourism e-Microentrepreneurial Self-Efficacy (TeMSE) scale, a 13-item scale that provides insights into individuals’ beliefs in their ability to successfully perform the various microentrepreneurial roles and tasks in the tourism e-business sector

Structural features and pro-inflammatory effects of water-soluble organic matter in inhalable fine urban air particles

The impact of inhalable fine particulate matter (PM2.5, aerodynamic diameter < 2.5 µm) on public health is of great concern worldwide. Knowledge on their harmful effects are mainly due to studies carried out with whole air particles, being the contribution of their different fractions largely unknown. Herein, a set of urban PM2.5 samples were collected during day and nighttime periods in Autumn and Spring, aiming to address the seasonal and day-night variability of water-soluble organic matter (WSOM) composition. In vitro analysis of oxidative and pro-inflammatory potential of WSOM samples was carried out in both acute (24 h) and chronic (3 weeks) exposure setups using Raw264.7 macrophages as cell model. Findings revealed that the structural composition of WSOM samples differs between seasons and in a day-night cycle. Cells exposure resulted in an increase in the transcription of the cytoprotective Hmox1 and pro-inflammatory genes Il1b and Nos2, leading to a moderate pro-inflammatory status. These macrophages showed an impaired capacity to subsequently respond to a strong pro-inflammatory stimulus such as bacterial lipopolysaccharide, which may implicate a compromised capacity to manage harmful pathogens. Further investigation on aerosol WSOM could help to constrain the mechanisms of WSOM-induced respiratory diseases and contribute to PM2.5 regulations.publishe

Chitosan and polyethylene glycol based membranes with antibacterial properties for tissue regeneration

The prevention of microbial infections associated with implantable medical devices and superficial wounds represents one of the main research strategies in the field of biomaterials. The present study reports on the development of composite membranes of Chitosan (CS)-Polyethylene glycol (PEG) matrix, incorporating particles of biphasic calcium phosphate (BCP), zinc oxide (ZnO) and copper oxide (CuO). The properties that are relevant for intended applications in tissue regeneration and antibacterial coatings of implants were assessed. It was found that the addition of 1% (w/w - relative to the mass of CS) of each metal oxide promoted satisfactory bacteriostatic activity and exhibited no cytotoxic effects towards the Vero cell line. The formation of bonds between the CS/PEG matrix and ionic species from the powders enhanced the cross-linking degree and mechanical properties of composite membranes in comparison to the non-doped membrane with the same polymer matrix (CS/PEG = 70/30%). A gradual degradation of the composite membranes over the immersion time in simulated body fluid (SBF) was accompanied by a continuous surface deposition of uniform apatite layer.publishe

Hyperprolinemia as a clue in the diagnosis of a patient with a psychiatric disorder

Background: Over the last few years, microdeletions of the 22q11.2 region responsible for DiGeorge syndrome, or velocardiofacial syndrome, have been increasingly related to neuropsychiatric disorders including schizophrenia and bipolar disorders. These signs seem to be related to certain genes located in the hemideleted region as the proline dehydrogenase (PRODH) and the catecholo-methyltransferase (COMT) genes. The PRODH or proline oxidase deficiency is responsible for hyperprolinemia type 1 (HPI) also causing psychiatric manifestations. Case Report: We describe a 17 year old boy with previous mild psychomotor and speech delay, mild cognitive impairment, and obsessive behaviours who started his adolescent psychiatric care presenting irritablemood and aggressive behaviour with schizophrenia symptoms that scored a “severely ill” level PANSS assessment. Symptoms got worse when he was treated with valproic acid and plasma aminoacids showing increase in alanine and proline, suggested a mitochondrial involvement of the proline metabolic pathway. Results: Mild dysmorphia suggested a possible 22q11.2 deletion genetically confirmed involving both the PRODH and COMT regions. HPI that can present with psychiatric features is however a recessive disorder and therefore the symptoms could not be solely explained by this genetic deletion. Additional investigations also showed disclosed a p.L289m (c.1865 T > A) mutation in the PRODH gene. Discussion: We believe that the association of this mutation together with the 22q11.2 deletion would lead to a decrease of functional protein. Although it may be difficult to diagnosis chromosomal abnormalities in patients with no clear malformations and mild dysmorphic features as in this patient we emphasize need to investigate the aetiology in patients with psychiatric symptoms, especially if they have other systemic manifestations such as developmental delay or psychotic symptoms, as it may be important in the management of the patients

Hyperprolinemia as a clue in the diagnosis of a patient with psychiatric manifestations

Lately, microdeletions of the 22q region, responsible for DiGeorge syndrome or velocardiofacial syndrome, have been increasingly related to neuropsychiatric disorders including schizophrenia and bipolar disorder. These manifestations seem to be related to certain genes located in the hemideleted region such as the proline dehydrogenase (PRODH) and the catechol-o-methyltransferase (COMT) genes. We describe a teenager who started his adolescent psychiatric care presenting cognitive impairment, irritable mood and aggressive behaviour with schizophrenia-like symptoms that scored 153 in the Positive and Negative Symptoms Scale (PANSS) assessment. Worsening of symptoms when the patient was treated with valproic acid, and plasma aminoacids showing an increase in alanine and proline, suggested a mitochondrial involvement of the proline metabolic pathway. Mild dysmorphic features also suggested a possible 22q11 deletion syndrome that was confirmed. A mutation for Hyperprolinemia type I was also detected. Knowledge of the correct diagnosis was crucial for an adequate treatment.info:eu-repo/semantics/publishedVersio

Implications of nanoparticles functionalization in supramolecular magnetogels for drug delivery

In this work, dehydropeptide gels were loaded with citrate- or lipid-stabilized nanoparticles to evaluate the effect over doxorubicin release. Particularly, the lipid-stabilized nanoparticles co-assembled with the hydrogel fibres forming lipid-fibre interface domains.Portugal2020, Compete2020, FEDER. SFRH/BD/144017/2019. PTDC/QUI-QFI/28020/2017. UIDB/04650/202

Tuning peptide-based hydrogels: co-assembly with composites driving the highway to technological applications

Self-assembled peptide-based gels provide several advantages for technological applications. Recently, the co-assembly of gelators has been a strategy to modulate and tune gel properties and even implement stimuli-responsiveness. However, it still comprises limitations regarding the required library of compounds and outcoming properties. Hence, efforts have been made to combine peptide-based gels and (in)organic composites (e.g., magnetic nanoparticles, metal nanoparticles, liposomes, graphene, silica, clay, titanium dioxide, cadmium sulfide) to endow stimuli-responsive materials and achieve suitable properties in several fields ranging from optoelectronics to biomedical. Herein, we discuss the recent developments with composite peptide-based gels including the fabrication, tunability of gels' properties, and challenges on (bio)technological applications.This work was funded by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding of CF-UM-UP (UIDB/04650/2020) and CQUM (UIDB/00686/2020), and by Ministerio de Economía y Competitividad de España (PID2020-113704RB-I00 and PID2020-119242RB-I00), Xunta de Galicia (Centro Singular de Investigación de Galicia—Accreditation 2019-2022 ED431G 2019/06 and IN607A 2018/5 and project ED431C 2020-06), and European Union (EU-ERDF Interreg V-A—Spain-Portugal 0245_IBEROS_1_E, 0712_ACUINANO_1_E, and 0624_2IQBIONEURO_6_E, and Interreg Atlantic Area NANOCULTURE 1.102.531), and the European Union H2020-MSCA-RISE-2019 PEPSA-MATE 872233 project. S.R.S. Veloso acknowledges FCT for a PhD grant (SFRH/BD/144017/2019). Support from MAP-Fis Doctoral Programme is also acknowledged