13 research outputs found
Effects of Aflatoxin B1 and Fumonisin B1 on the Viability and Induction of Apoptosis in Rat Primary Hepatocytes
The present study evaluated the effect of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) either alone, or in association, on rat primary hepatocyte cultures. Cell viability was assessed by flow cytometry after propidium iodine intercalation. DNA fragmentation and apoptosis were assessed by agarose gel electrophoresis and acridine orange and ethidium bromide staining. At the concentrations of AFB1 and FB1 used, the toxins did not decrease cell viability, but did induce apoptosis in a concentration and time-dependent manner
Exploring marine ecosystems with elementary school Portuguese children: inquiry-based project activities focused on ‘real-life’ contexts
The purpose of the study was to investigate how young students engage in an inquirybased
project driven by real-life contexts. Elementary school children were engaged in a
small inquiry project centred on marine biodiversity and species adaptations. All
activities included the exploration of an out-of-school setting as a learning context. A
total of 49 students and 2 teachers were involved in the activities. The research
methods included observation, document analysis and content analysis of the answers
to a questionnaire and an interview. The results revealed that most of the students
acquired scientific knowledge related to biological diversity and adaptations to
habitat. Moreover, students progressively demonstrate greater autonomy,
argumentative ability and decision-making. One implication of the present study is
that elementary science curriculum could be better managed with inquiry projectbased
activities that explore different types of resources and out-of-school settings.info:eu-repo/semantics/publishedVersio