224 research outputs found
In situ spectroellipsometric study of the nucleation and growth of amorphous silicon
A detailed in situ spectroellipsometric analysis of the nucleation and growth of hydrogenated amorphous silicon (a:Si:H) is presented. Photoelectronic quality aâSi:H films are deposited by plasmaâenhanced chemical vapor deposition on smooth metal (NiCr alloy) and crystalline silicon (câSi) substrates. The deposition of aâSi:H is analyzed from the first monolayer up to a final thickness of 1.2 ÎŒm. In order to perform an improved analysis, real time ellipsometric trajectories are recorded, using fixed preparation conditions, at various photon energies ranging from 2.2 to 3.6 eV. The advantage of using such a spectroscopic experimental procedure is underlined. New insights into the nucleation and growth mechanisms of aâSi:H are obtained. The nucleation mechanism on metal and câSi substrates is very accurately described assuming a columnar microstructural development during the early stage of the growth. Then, as a consequence of the incomplete coalescence of the initial nuclei, a surface roughness at the 10-15 Ă
scale is identified during the further growth of aâSi:H on both substrates. The bulk aâSi:H grows homogeneously beneath the surface roughness. Finally, an increase of the surface roughness is evidenced during the long term growth of aâSi:H. However, the nature of the substrate influenced the film growth. In particular, the film thickness involved in the nucleationâcoalescence phase is found lower in the case of câSi (67±8 Ă
) as compared to NiCr (118±22 Ă
). Likewise films deposited on câSi present a smaller surface roughness even if thick samples are considered (>1 ÎŒm). More generally, the present study illustrates the capability of in situ spectroellipsometry to precisely analyze fundamental processes in thinâfilm growth, but also to monitor the preparation of complex structures on a few monolayers scale
Stable microcrystalline silicon thin-film transistors produced by the layer-by-layer technique
International audienceMicrocrystalline siliconthin films prepared by the layer-by-layer technique in a standard radio-frequency glow discharge reactor were used as the active layer of top-gate thin-film transistors(TFTs). Crystalline fractions above 90% were achieved for silicon films as thin as 40 nm and resulted in TFTs with smaller threshold voltages than amorphous siliconTFTs, but similar field effect mobilities of around 0.6 cm2/Vâs. The most striking property of these microcrystalline silicontransistors was their high electrical stability when submitted to bias-stress tests. We suggest that the excellent stability of these TFTs, prepared in a conventional plasma reactor, is due to the stability of the ÎŒc-Si:H films. These TFTs can be used in applications that require high stability for which a-Si:HTFTs cannot be used, such as multiplexed row and column drivers in flat-panel display applications, and active matrix addressing of polymer light-emitting diodes
CSN5 binds to misfolded CFTR and promotes its degradation
AbstractCystic fibrosis is mainly caused by mutations that interfere with the biosynthetic folding of the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The aim of this study was to find cellular proteins interacting with CFTR and regulating its processing. We have used a genetic screen in yeast to identify such proteins and identified CSN5 that interacted with the third cytoplasmic loop of CFTR. CSN5 is the 5th component of the COP9 signalosome, a complex of eight subunits that shares significant homologies to the lid subcomplex of the 26S proteasome and controls the stability of many proteins. The present study shows that CSN5 associates with the core-glycosylated form of CFTR and suggests that this association targets misfolded CFTR to the degradative pathway. Identifying CSN5 as a new component of the degradative pathway is an important step towards the goal of unraveling the sorting between misfolded and correctly folded CFTR proteins
Characterisation of nanostructured GaSb : Comparison between large-area optical and local direct microscopic techniques
Low energy ion-beam sputtering of GaSb results in self-organized
nanostructures, with the potential of structuring large surface areas.
Characterisation of such nanostructures by optical methods is studied and
compared to direct (local) microscopic methods. The samples consist of densely
packed GaSb cones on bulk GaSb, approximately 30, 50 and 300 nm in height,
prepared by sputtering at normal incidence. The optical properties are studied
by spectroscopic ellipsometry, in the range 0.6-6.5 eV, and with Mueller matrix
ellipsometry in the visible range, 1.46-2.88 eV. The optical measurements are
compared to direct topography measurements obtained by Scanning Electron
Microscopy (SEM), High Resolution Transmission Electron Microscopy (HR-TEM),
and Atomic Force Microscopy (AFM). Good agreement is achieved between the two
classes of methods when the experimental optical response of the short cones
(<55 nm) is inverted with respect to topological surface information via a
graded anisotropic effective medium model. The main topological parameter
measured was the average cone height, but estimates of typical cone shape and
density (in terms of volume fractions) were also obtained. The graded
anisotropic effective medium model treats the cones as a stack of concentric
cylinders (discs) of non-increasing radii. Optical methods are shown to
represent a valuable characterization tool for nanostructured surfaces, in
particular when large coverage area is desirable. Due to the fast and
non-destructive properties of optical techniques, they may readily be adapted
to in-situ configurations
COMMD1-Mediated Ubiquitination Regulates CFTR Trafficking
The CFTR (cystic fibrosis transmembrane conductance regulator) protein is a large polytopic protein whose biogenesis is inefficient. To better understand the regulation of CFTR processing and trafficking, we conducted a genetic screen that identified COMMD1 as a new CFTR partner. COMMD1 is a protein associated with multiple cellular pathways, including the regulation of hepatic copper excretion, sodium uptake through interaction with ENaC (epithelial sodium channel) and NF-kappaB signaling. In this study, we show that COMMD1 interacts with CFTR in cells expressing both proteins endogenously. This interaction promotes CFTR cell surface expression as assessed by biotinylation experiments in heterologously expressing cells through regulation of CFTR ubiquitination. In summary, our data demonstrate that CFTR is protected from ubiquitination by COMMD1, which sustains CFTR expression at the plasma membrane. Thus, increasing COMMD1 expression may provide an approach to simultaneously inhibit ENaC absorption and enhance CFTR trafficking, two major issues in cystic fibrosis
Influence of Rossby waves on primary production from a coupled physical-biogeochemical model in the North Atlantic Ocean
Rossby waves appear to have a clear signature on surface chlorophyll concentrations which can be explained by a combination of vertical and horizontal mechanisms. In this study, we investigate the role of the different physical processes in the north Atlantic to explain the surface chlorophyll signatures and the consequences on primary production, using a 3-D coupled physical/biogeochemical model for the year 1998. <br><br> The analysis at 20 given latitudes, mainly located in the subtropical gyre, where Rossby waves are strongly correlated with a surface chlorophyll signature, shows the important contribution of horizontal advection and of vertical advection and diffusion of inorganic dissolved nitrogen. The main control mechanism differs according to the biogeochemical background conditions of the area. <br><br> The surface chlorophyll anomalies, induced by these physical mechanisms, have an impact on primary production. We estimate that Rossby waves induce, locally in space and time, increases (generally associated with the chlorophyll wave crest) and decreases (generally associated with the chlorophyll wave trough) in primary production, ~&plusmn;20% of the estimated background primary production. This symmetrical situation suggests a net weak effect of Rossby waves on primary production
Structural Determination of Nanocrystalline Si Films Using Ellipsometry and Raman Spectroscopy
Single phase nano and micro crystalline silicon films deposited using SiF4/H2
plasma at different H2 dilution levels were studied at initial and terminal
stages of film growth with spectroscopic ellipsometry (SE), Raman scattering
(RS) and atomic force microscopy (AFM). The analysis of data obtained from SE
elucidates the microstructural evolution with film growth in terms of the
changes in crystallite sizes and their volume fractions, crystallite
conglomeration and film morphology. The effect of H2 dilution on film
microstructure and morphology, and the corroborative findings from AFM studies
are discussed. Our SE results evince two distinct mean sizes of crystallites in
the material after a certain stage of film growth. The analysis of Raman
scattering data for such films has been done using a bimodal size distribution
of crystallite grains, which yields more accurate and physically rational
microstructural picture of the material.Comment: 5 pages, 4 figures, 1 tabl
SCUBA divers as oceanographic samplers: The potential of dive computers to augment aquatic temperature monitoring
Monitoring temperature of aquatic waters is of great importance, with modelled, satellite and in-situ data providing invaluable insights into long-term environmental change. However, there is often a lack of depth-resolved temperature measurements. Recreational dive computers routinely record temperature and depth, so could provide an alternate and highly novel source of oceanographic information to fill this data gap. In this study, a citizen science approach was used to obtain over 7,000 scuba diver temperature profiles. The accuracy, offset and lag of temperature records was assessed by comparing dive computers with scientific conductivity-temperature-depth instruments and existing surface temperature data. Our results show that, with processing, dive computers can provide a useful and novel tool with which to augment existing monitoring systems all over the globe, but especially in under-sampled or highly changeable coastal environments
- âŠ