66 research outputs found
Protection of a lens of an eye against the simulated diabetic cataract
ΠΠ°ΡΠ°ΡΠ°ΠΊΡΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΡΠ΅Π½Ρ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΠΌ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠΈ Π΄ΠΈΠ°Π±Π΅ΡΠ΅. ΠΡ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π»ΠΈ Π±ΡΡΠΈΠ΅ Π»ΠΈΠ½Π·Ρ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π²ΡΡΠΎΠΊΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π³Π»ΡΠΊΠΎΠ·Ρ (450 ΠΌΠ³.%) Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΊΡΠ»ΡΡΡΡΡ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ Π΄Π²ΡΡ
Π½Π΅Π΄Π΅Π»Ρ ΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΠΈ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡ Π² Π»ΠΈΠ½Π·Π΅ ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΡΡ Π·Π°ΡΠΈΡΡ ΡΠΏΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠΌΠΈ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ°ΠΌΠΈ N Π°ΡΠ΅ΡΠΈΠ» L ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠΌ (NAC) ΠΈ ΡΠΈΠ½ΠΊΠΎΠ²ΡΠΌ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠΌ desferrioxamine (DFO), ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΡΠΌ Ρ
Π΅Π»Π°ΡΠΎΡΠΎΠΌ Π΄Π»Ρ ΠΆΠ΅Π»Π΅Π·Π°. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΠΎΡΡ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎ Π»ΠΈΠ½Π· ΠΈ ΠΎΠΊΠΈΡΠ»Π΅Π½ΠΈΠ΅ ΡΠΏΠΈΡΠ΅Π»ΠΈΡ Ρ Π΄ΠΈΡ
Π»ΠΎΡΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅ΠΈΠ½ΠΎΠΌ (DCF), Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² 2D Π³Π΅Π»Ρ
ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΏΡΠΎΡΠΈΠ»Π΅ Π±Π΅Π»ΠΊΠΎΠ² Ρ
ΡΡΡΡΠ°Π»ΠΈΠΊΠ°. ΠΠ°Π±Π»ΡΠ΄Π°Π»ΠΈΡΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΏΡΠΈ Π²ΡΡΠΎΠΊΠΎΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠΈ Π³Π»ΡΠΊΠΎΠ·Ρ Π² ΡΠΎΠΊΡΡΠ½ΠΎΠΌ ΡΠ°ΡΡΡΠΎΡΠ½ΠΈΠΈ Π»ΠΈΠ½Π·Ρ, ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠΈ ΠΎΠΊΠΈΡΠ»Π΅Π½ΠΈΡ. NAC Π Zn DFO ΠΏΠΎΡΡΠΈ ΠΏΠΎΠ»Π½ΠΎΡΡΡΡ Π·Π°ΡΠΈΡΠ°Π»ΠΈ Π»ΠΈΠ½Π·Ρ; DFO ΠΏΠΎΠΊΠ°Π·ΡΠ²Π°Π» ΡΠΎΠ»ΡΠΊΠΎ ΡΠ°ΡΡΠΈΡΠ½ΡΡ Π·Π°ΡΠΈΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π»ΠΈ, ΡΡΠΎ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΡ ΠΌΠΎΠ³ΡΡ Π·Π°ΡΠΈΡΠ°ΡΡΡ Ρ
ΡΡΡΡΠ°Π»ΠΈΠΊ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π°ΡΡΠ΅Π³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π²ΡΡΠΎΠΊΠΈΡ
ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΉ Π³Π»ΡΠΊΠΎΠ·Ρ. ΠΠΎΠΌΠ±ΠΈΠ½Π°ΡΠΈΡ NAC ΠΈ ZnDFO Π΄Π΅ΠΉΡΡΠ²ΠΎΠ²Π°Π»Π° Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ.ΠΠ°ΡΠ°ΡΠ°ΠΊΡΠ° Ρ Π΄ΡΠΆΠ΅ ΠΏΠΎΡΠΈΡΠ΅Π½ΠΈΠΌ ΡΡΠΊΠ»Π°Π΄Π½Π΅Π½Π½ΡΠΌ ΠΏΡΠΈ Π΄ΡΠ°Π±Π΅ΡΡ. ΠΠΈ ΠΏΡΠ΄Π΄Π°Π»ΠΈ ΠΊΡΠΈΡΡΠ°Π»ΠΈΠΊ ΠΎΠΊΠ° Π±ΠΈΠΊΠ° Π΄ΡΡ Π²ΠΈΡΠΎΠΊΠΎΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΡΡ Π³Π»ΡΠΊΠΎΠ·ΠΈ (450 ΠΌΠ³ %) Π² ΡΠΌΠΎΠ²Π°Ρ
ΠΊΡΠ»ΡΡΡΡΠΈ ΠΏΡΠΎΡΡΠ³ΠΎΠΌ Π΄Π²ΠΎΡ
ΡΠΈΠΆΠ½ΡΠ² Ρ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π»ΠΈ ΠΏΠΎΡΠΊΠΎΠ΄ΠΆΠ΅Π½Π½Ρ Π² Π»ΡΠ½Π·Ρ Ρ ΠΌΠΎΠΆΠ»ΠΈΠ²ΠΈΠΉ Π·Π°Ρ
ΠΈΡΡ ΡΠΏΠ΅ΡΡΠ°Π»ΡΠ½ΠΈΠΌΠΈ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ°ΠΌΠΈ N Π°ΡΠ΅ΡΠΈΠ» L ΡΠΈΡΡΠ΅ΠΈΠ½ΠΎΠΌ (NAC) Ρ ΡΠΈΠ½ΠΊΠΎΠ²ΠΈΠΌ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠΌ desferrioxamine (DFO), ΡΠ΅Π»Π΅ΠΊΡΠΈΠ²Π½ΠΈΠΌ Ρ
Π΅Π»Π°ΡΠΎΡΠΎΠΌ Π΄Π»Ρ Π·Π°Π»ΡΠ·Π°. ΠΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π»Π°ΡΡ ΠΎΠΏΡΠΈΡΠ½Π° ΡΠΊΡΡΡΡ Π»ΡΠ½Π· Ρ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½Ρ Π΅ΠΏΡΡΠ΅Π»ΡΡ Π· Π΄ΠΈΡ
Π»ΠΎΡΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅ΡΠ½ΠΎΠΌ (DCF), Π° ΡΠ°ΠΊΠΎΠΆ ΠΎΡΡΠ½ΡΠ²Π°Π»ΠΈΡΡ Π·ΠΌΡΠ½ΠΈ Π² 2D Π³Π΅Π»Ρ Π΅Π»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅ΡΠΈΡΠ½ΠΎΠΌΡ ΠΏΡΠΎΡΡΠ»Ρ Π±ΡΠ»ΠΊΡΠ² ΠΊΡΠΈΡΡΠ°Π»ΠΈΠΊΠ°. Π‘ΠΏΠΎΡΡΠ΅ΡΡΠ³Π°Π»ΠΈΡΡ Π·ΠΌΡΠ½ΠΈ ΠΏΡΠΈ Π²ΠΈΡΠΎΠΊΠΎΠΌΡ Π²ΠΌΡΡΡΡ Π³Π»ΡΠΊΠΎΠ·ΠΈ Ρ ΡΠΎΠΊΡΡΠ½ΡΠΉ Π²ΡΠ΄ΡΡΠ°Π½Ρ Π»ΡΠ½Π·ΠΈ, ΠΏΡΠ΄Π²ΠΈΡΠ΅Π½Π½Ρ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½Ρ. NAC Π Zn DFO ΠΌΠ°ΠΉΠΆΠ΅ ΠΏΠΎΠ²Π½ΡΡΡΡ Π·Π°Ρ
ΠΈΡΠ°Π»ΠΈ Π»ΡΠ½Π·ΠΈ; DFO ΠΏΠΎΠΊΠ°Π·ΡΠ²Π°Π² ΡΡΠ»ΡΠΊΠΈ ΡΠ°ΡΡΠΊΠΎΠ²ΠΈΠΉ Π·Π°Ρ
ΠΈΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ Π΄Π΅ΠΌΠΎΠ½ΡΡΡΡΠ²Π°Π»ΠΈ, ΡΠΎ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠΈ ΠΌΠΎΠΆΡΡΡ Π·Π°ΡΠΈΡΠ°ΡΡΡ ΠΊΡΠΈΡΡΠ°Π»ΠΈΠΊ Π²ΡΠ΄ ΡΡΠΊΠΎΠ΄ΠΆΡΠ²Π°Π»ΡΠ½ΠΎΡ Π΄ΡΡ Π²ΠΈΡΠΎΠΊΠΈΡ
ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΡΠΉ Π³Π»ΡΠΊΠΎΠ·ΠΈ. ΠΠΎΠΌΠ±ΡΠ½Π°ΡΡΡ NAC Ρ ZNDFO Π΄ΡΡΠ»Π° Π΅ΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡΠ΅
Simulations of Photon Detection in SiPM Number-Resolving Detectors
Number-resolving single photon detectors are essential for the implementation
of numerous innovative quantum information schemes. While several
number-discriminating techniques have been previously presented, the Silicon
Photo-Multiplier (SiPM) detector is a promising candidate due its rather simple
integration in optical setups. On the other hand, the photon statistics
obtained with the SiPM detector suffer from inaccuracies due to inherent
distortions which depend on the geometrical properties of the SiPM. We have
simulated the detection process in a SiPM detector and studied these
distortions. We use results from the simulation in order to interpret
experimental data and study the limits in which available models prevail
Subtle Alterations in PCNA-Partner Interactions Severely Impair DNA Replication and Repair
Dynamic switching of PCNA-partner interactions is essential for normal DNA replication and repair in yeast
PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions
Proliferating cell nuclear antigen (PCNA) is an essential cofactor for DNA replication and repair, recruiting multiple proteins to their sites of action. We examined the effects of the PCNA(S228I) mutation that causes PCNA-associated DNA repair disorder (PARD). Cells from individuals affected by PARD are sensitive to the PCNA inhibitors T3 and T2AA, showing that the S228I mutation has consequences for undamaged cells. Analysis of the binding between PCNA and PCNA-interacting proteins (PIPs) shows that the S228I change dramatically impairs the majority of these interactions, including that of Cdt1, DNMT1, PolD3(p66) and PolD4(p12). In contrast p21 largely retains the ability to bind PCNA(S228I). This property is conferred by the p21 PIP box sequence itself, which is both necessary and sufficient for PCNA(S228I) binding. Ubiquitination of PCNA is unaffected by the S228I change, which indirectly alters the structure of the inter-domain connecting loop. Despite the dramatic in vitro effects of the PARD mutation on PIP-degron binding, there are only minor alterations to the stability of p21 and Cdt1 in cells from affected individuals. Overall our data suggests that reduced affinity of PCNA(S228I) for specific clients causes subtle cellular defects in undamaged cells which likely contribute to the etiology of PARD
Mutations at the Subunit Interface of Yeast Proliferating Cell Nuclear Antigen Reveal a Versatile Regulatory Domain
Acknowledgments We thank Szilvia Minorits for technical assistance. I.U. conceived and designed the project and wrote the manuscript. All authors participated in designing and performing the experiments, and analyzing the results. The authors declare no competing financial interests. This work was also supported by a grant from the National Research, Development and Innovation Office GINOP-2.3.2-15-2016-00001. Funding: This work was supported by Hungarian Science Foundation Grant OTKA 109521 and National Research Development and Innovation Office GINOP-2.3.2-15-2016-00001. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD
- β¦