Short term response of grapevine grown hydroponically to salinity: Mineral composition and growth parameters

The response of hydroponically grown four ownrooted table grape (Vitis vinifera L.) cultivars ('Red Rishbaba', 'Red Sahebi', 'Dastarchin' and 'Red Sultana') to different salt concentrations (0, 25, 50 and 100 mM NaCl) was studied under greenhouse condition. Growth parameters, total chlorophyll (a+b) and proline contents were determined in leaves and roots. Cl-, Na+, K+ and NO3 - concentrations were measured in lamina, petiole, stem and root. Shoot growth, total dry weight, total leaf chlorophyll (a+b), NO3 --N and K+ contents were significantly reduced (P≤0.05) under NaCl stress, whereas proline, Cl- and Na+ accumulation increased significantly with increasing salinity. 'Red Rishbaba' and 'Red Sahebi' showed a less decrease in total leaf chlorophyll, K+ and NO3 - contents, while proline accumulation in these cultivars was higher than that of 'Dastarchin' and 'Red Sultana'. Also, shoots of 'Red Sahebi' and 'Red Rishbaba' accumulated lower Cl- and Na+ than other cultivars. In conclusion, the overall results showed that 'Red Rishbaba' and 'Red Sahebi' were more tolerant than 'Dastarchin' and 'Red Sultana'.

Leaf water potential, photosynthetic pigments and compatible solutes alterations in four grape cultivars under salinity

A hydroponic culture experiment was conducted to investigate the effects of different concentrations (0, 25, 50 and 100 mM) NaCl on own-rooted table grape (Vitis vinifera L.) cultivars (red 'Rishbaba', red 'Sahebi', 'Dastarchin' and red 'Sultana') under greenhouse conditions. Cultivars were evaluated for growth analysis leaf area, leaf water potential and the chlorophyll a, b and carotenoid contents in relation to proline and soluble sugars accumulation. Salinity treatments caused a growth reduction (P ≤ 0.05) in all the cultivars. Also leaf water potential and chlorophyll a, b contents decreased whereas carotenoid, proline and soluble sugars increased with increasing NaCl concentration. 'Dastarchin' and red 'Sultana' showed the salt- sensitivity, the highest loss of growth, leaf water potential and chlorophyll content and the lowest accumulation of carotenoids, proline and soluble sugars. Also salt stress significantly (P < 0.001) increased the rate of lipid peroxidation in the all cultivars particularly in 'Dastarchin' and red 'Sultana'. The increase in malondialdehyde content indicated that salinity induced oxidative stress. There was a significant negative correlation between leaf water potential and NaCl concentrations (r2: -0.781, p < 0.001). A positive correlation was also found between lamina proline contents and NaCl concentrations (r2: +0.964, p < 0.001) for all salinity treatments. Considering overall results red 'Rishbaba' and red 'Sahebi' showed higher capacity to tolerate salinity when compared to 'Dastarchin' and red 'Sultana'.

Report of a new mutation and frequency of connexin 26 gene (GJB2) mutations in patients from three provinces of Iran

Autosomal recessive and sporadic non-syndromic hearing loss (ARSNSHL) is the major form of hereditary deafness. Mutations in the GJB2 gene encoding the gap-junction protein Connexin 26 have been identified to be highly associated with ARSNSHL. In this study we have analyzed 196 deaf subjects from 179 families having one or more deaf children in 3 proviences of Iran, including Kordestan, Khuzestan and Golestan. The nested PCR prescreening strategy and direct sequencing technique were used to detect the mutations in coding exon of the gene. Altogether 3 GJB2 recessive mutations including 35delG, 167delT and V27I+E114G, were identified in 23 of 179 families (12.8). Fourteen of 179 families were observed to have GJB2 mutation in both alleles (7.8). A novel variant (R159H) also was found in a deaf family from Khuzestan. Four polymorphisms V27I, E114G, S86T and V153 I also were detected in 7 families. A polymorphism(S86T) was seen in the whole population studied. Our data indicated that the rate of connexin 26 mutations is different in this three Irainian population and is lower than the high frequency of 35delG (26) reported from Gilan province in the north of Iran

Analysis of SSR and AFLP Markers to Detect Genetic Diversity Among Selected Clones of Grapevine (Vitis vinifera L.) cv. Keshmeshi

To assess the genetic differences between clones of grapevine (Vitis vinifera L.) cv. Keshmeshi, ten selected clones from a clonal selection programme were analysed by 23 Simple Sequence Repeats (SSR) and seven AFLP primer combinations. No intra-varietal differences between the clones could be detected by SSRs, whereas eight out of the 499 AFLP fragments generated by the seven primer combinations were polymorphic. The number of markers ranged from 44 (E34-M34) to 97 (E31-M32), with an average of 71.3 fragments per primer combination. Cluster analysis based on the AFLP data separated all the clones of Keshmeshi in two groups. The first group included nine white berry skin clones without any genetic differences, and the second group with only a red berry skin clone. AFLP could only distinguish the red berry clone of Keshmeshi from other white berry clones

Amount of Macronutrients and Micronutrients in Petiole of Some Iranian and Imported Grape Cultivars

Introduction: Grapevine (Vitis vinifera L.) is one of the oldest and most important perennial crops in the world. Several native grapevine genotypes, highly appreciated for their organoleptic characteristics and commercial potential are still cultivated in Iran. Developing viticulture requires the conservation of autochthonous varieties that have evolved several mechanisms enabling them to cope with the local bioclimatic and edaphic conditions. Nutrition is a key component of vineyard management that has the potential to influence various factors in vine production that includes fruit set and quality. To develop suitable nutrient plant growers need to have an understanding of the factors such as cultivars, rootstocks, soil type, irrigation type and nutrients that they are applying in the vineyard. The uptake of nutrients from the soil depends on different factors namely; their soluble content in it, soil pH, plant growth stage, plant genetics and types of soil and fertilizers. Plant species have a variety of capacities in removing and accumulating elements. Vigorous genotypes are more capable of finding the necessary nutrients from the surrounding soil environment. This indicates that it does not require as much nutrient as poor vigor genotypes. So, for sustainable viticulture, it is important to know the interactive influences of cultivars, soil characters, climatic conditions, and irrigation type on vine productivity. Materials and Methods: To evaluate and compare the amount of macronutrient elements (N, P, K, Mg and Ca) and micronutrient elements (B, Zn and Mn) in petiole of some Iranian grapevine cultivars including Bidanesefid Qazvin- Peikany Kashmar- Khalili Shiraz-Rasha and four foreign cultivars Thompson seedless, Flame seedless, Perlette and Black seedless,This study was carried out as –randomized complete blocks design -with-four replications in the Kahriz Horticulture Research station -.A total of 30 adult leaves per cultivar were taken from lower, middle and upper regions of the vines bulked together and transported directly to the laboratory. They were oven-dried for 48 h at 70 °C and grounded to pass through a 1 mm diameter sieve. The concentrations of the -mineral elements were determined using an atomic absorption and spectrophotometer. Results and Discussion: The results showed there was significant difference among study cultivars in respect of elements concentration in petiole-. Among 8 cultivars, the highest and the lowest petiole N concentration were recorded in Flame seedless-and Peikany and Rasha cultivars respectively. P -concentration in Bidane sefid Qazvin was significantly higher than all tested cultivars. The highest and the lowest Mg amounts were measured in Peikany and Bidanesefid Qazvin, respectively. In petiole of cultivars B concentration was in the range of toxicity except Rasha that had the concentration less than -other cultivars. Iranian cultivars had lesscap ability to absorb Zn than abroad cultivars. The highest and lowest Zn were recorded in petioles of Thompson seedless and Peikany cultivars, respectively. It was reported that the mineral content of a grapevine is a combined result of the root systems ability to absorb, trans locate and accumulate the different nutrients. Previous investigations had clearly stated the differences in nutrients uptake and content of many grape cultivars. Furthermore, grape cultivars have shown differences in their nutrients uptake and distribution. These differences may be explained in different ways. First, cultivar may have different absorption capability or tendency for some specific minerals. Second, differences exist in translocation and distribution of nutrients and third, hormone synthesis of cultivar roots and their translocation is done. Finally, some nutrients might be assimilated mostly by roots; thus reducing the amount translocated to the shoots. In addition, some grape varieties may alter soil chemical characters and play a role in improving nutrients uptake. The rootstocks of V. labruscaand scions grafted on them achieve a higher ability in uptaking iron, even in markedly alkaline soils. Such tolerant varieties can mobilize iron by reducing soil pH at root level, thanks to their ability to emit H+ and/or organic acids.In the latter case, iron is absorbed and transferred as a complex. Roots of some cultivars can also reduce Fe3+ to Fe2 + encouraging its migration from roots to leaves. Conclusion: The studied grapevine cultivars displayed a considerable level of variability based on mineral content analysis. The results suggested that significant differences existed in the leaf petioles elemental concentrations among the grapevine cultivars analysis that might be in due part to the ability of the cultivar to accumulate metals. This study could be also used as a reference for grape growers to help them decide the best varieties that might grow under their soil conditions giving the best growth and yield productivity

Study of Genetic Relationships Between Wild and Domesticated Grapevine Distributed from Middle East Regions to European Countries

Archaeobotanical-archaeological, cultural and historical data indicate that grapevine domestication can be dated back from 6000 to 7000 years ago and that it took place in the Caucasian and Middle East Regions. However, events leading to the domestication of this crop species are still an open issue. In this paper, 6 chloroplast microsatellites have been used to assess genetic similarities among, and within, domesticated and wild grapevine accessions representative of 7 distinct geographical regions from the Middle-East to Western Europe. Results show that 2 out of the 6 analyzed chloroplast loci are polymorphic within the 193 domesticated individuals and the 387 samples of 69 wild populations. Allele variants of the Cp-SSR loci combine in a total of 6 different haplotypes. The data show that the haplotype distribution is not homogeneous: the 6 haplotypes are present in the domesticated varieties, but only 5 (haplotype VI is absent) are observed in wild populations. The analysis of haplotype distribution allows discussion of the relationships between the two grape subspecies. The contribution of the wild grape germplasm to the domesticated gene pool still growing in different geographical regions can be, in cases, made evident, suggesting that beside domestication, gene introgression has also played a role in shaping the current varietal landscape of the European viticulture

A combined mathematical geophysical model for prediction of pyrite oxidation and pollutant leaching associated with a coal washing waste dump

The waste produced by coal washing process produces many environmental problems. In this study, the pollution problems associated with the waste produced by Alborz Sharghi Coal Washing Plant was investigated by mathematical modeling. The study area is located at 11 km. to Razmjah coal region and 45 km. to Tehran-Mashhad road in the north part od Iran. To achieve the goal, a few samples were taken from different depths at three points on the waste dump in order to investigate pyrite oxidation and pollution generation. The samples were then analysed, using an AA670 Shimadzu atomic absorption to determine the fraction of pyrite remained within the waste particles. A numerical finite volume model using Phoenics package has been developed to simulate pyrite oxidation and pollution generation from the Alborz Sharghi coal washing waste dump. The pyrite oxidation reaction is described by the shrinking-core model. Gaseous diffusion is the main mechanism for the transport of oxygen through the waste. The results of numerical modelling were compared with the field observations and close agreement was achieved. A simple mathematical model incorporating advection and hydrodynamic dispersion processes was also presented in order to verify the results of geophysical time-laps method showing transportation of the pollutants through the downstream of the waste dump. Both mathematical model and geophysical time-laps method are agreed in the identification of pollutant transport emanated from the waste dump. The results of such investigations can be used for designing an effective environmental management program

Prediction of terminal velocity of solid spheres falling through Newtonian and non-Newtonian pseudoplastic power law fluid using artificial neural network

Δημοσίευση σε επιστημονικό περιοδικόSummarization: Prediction of the terminal velocity of solid spheres falling through Newtonian and non-Newtonian fluids is required in several applications like mineral processing, oil well drilling, geothermal drilling and transportation of non-Newtonian slurries. An artificial neural network (ANN) is proposed which predicts directly the terminal velocity of solid spheres falling through Newtonian and non-Newtonian power law liquids from the knowledge of the properties of the spherical particle (density and diameter) and of the surrounding liquid (density and rheological parameters). With a combination of non-Newtonian data with Newtonian data taken from published data giving a database of 88 sets, an artificial neural network is designed. Analysis of the predictions shows that the artificial neural network could be used with good engineering accuracy to directly predict the terminal velocity of solid spheres falling through Newtonian and non-Newtonian power law liquids covering an extended range of power law values from 1.0 down to 0.06.Presented on: International Journal of Mineral Processin