599 research outputs found
Eight decades of adaptive changes in herring reproductive investment: the joint effect of environment and exploitation
publishedVersio
Effect of environmental temperature during the of brooding period on growing period of pullets viscera and tibia
ArticlePoultry production in subtropical and tropical regions faces many problems, one of
which is the high air temperature causing thermal stress, particularly dangerous in high-producing
birds. Thus, the negative effects caused by heat stress (HS) must be managed. The objective of
this study was to evaluate the effects of four different levels of HS in viscera and tibia of pullets.
A total of 648 chicks (Lohmann LSL Lite) were used in this study in two different phases. The
pre-experimental phase (PEP) was from day 1 through 6 weeks of age. The birds were reared
with three different environmental temperatures: thermal comfort, hot and cold. The experimental
phase (EP) was conducted from the 7th to the 17th week. Pullets from each thermal environment
of the PEP were submitted to: 20 °C, 25 °C, 30 °C, 35 °C. At the end of the 17th week of age 120
pullets were euthanatized and the organs, heart, liver, spleen and gizzard were weighed, as also
their tibias. Effects of PEP, and its interaction with EP, were not significant (P < 0.05) for viscera
and tibia weight. However, a significant increase (P < 0.05) in heart weight with the decrease of
the environmental temperature was observed, being the pullets subject to 20ºC and 25 °C with
the heaviest weights. For the liver, pullets subject to the 35 °C had the lowest weight and were
different (P < 0.05) from the other three treatments. For gizzard, the difference (P < 0.05) was
between the treatments 20ºC and 35 °C. These results indicate that brooding temperatures tested
during the first 6 weeks of life did not affect the viscera and bone weight during the growing
phase
Photoionization of ultracold and Bose-Einstein condensed Rb atoms
Photoionization of a cold atomic sample offers intriguing possibilities to
observe collective effects at extremely low temperatures. Irradiation of a
rubidium condensate and of cold rubidium atoms within a magneto-optical trap
with laser pulses ionizing through 1-photon and 2-photon absorption processes
has been performed. Losses and modifications in the density profile of the
remaining trapped cold cloud or the remaining condensate sample have been
examined as function of the ionizing laser parameters. Ionization
cross-sections were measured for atoms in a MOT, while in magnetic traps losses
larger than those expected for ionization process were measured.Comment: 9 pages, 7 figure
Metastable neon collisions: anisotropy and scattering length
In this paper we investigate the effective scattering length of
spin-polarized Ne*. Due to its anisotropic electrostatic interaction, its
scattering length is determined by five interaction potentials instead of one,
even in the spin-polarized case, a unique property among the Bose condensed
species and candidates. Because the interaction potentials of Ne* are not known
accurately enough to predict the value of the scattering length, we investigate
the behavior of as a function of the five phase integrals corresponding to
the five interaction potentials. We find that the scattering length has five
resonances instead of only one and cannot be described by a simple gas-kinetic
approach or the DIS approximation. However, the probability for finding a
positive or large value of the scattering length is not enhanced compared to
the single potential case. The complex behavior of is studied by comparing
a quantum mechanical five-channel numerical calculation to simpler two-channel
models. We find that the induced dipole-dipole interaction is responsible for
coupling between the different |\Omega> states, resulting in an inhomogeneous
shift of the resonance positions and widths in the quantum mechanical
calculation as compared to the DIS approach. The dependence of the resonance
positions and widths on the input potentials turns out to be rather
straightforward. The existence of two bosonic isotopes of Ne* enables us to
choose the isotope with the most favorable scattering length for efficient
evaporative cooling towards the Bose-Einstein Condensation transition, greatly
enhancing the feasibility to reach this transition.Comment: 13pages, 8 eps figures, analytical model in section V has been
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IKKβ targeting reduces KRAS-induced lung cancer angiogenesis in vitro and in vivo: A potential anti-angiogenic therapeutic target
Objectives: The ability of tumor cells to drive angiogenesis is an important cancer hallmark that positively correlates with metastatic potential and poor prognosis. Therefore, targeting angiogenesis is a rational therapeutic approach and dissecting proangiogenic pathways is important, particularly for malignancies driven by oncogenic KRAS, which are widespread and lack effective targeted therapies. Based on published studies showing that oncogenic RAS promotes angiogenesis by upregulating the proangiogenic NF-κB target genes IL-8 and VEGF, that NF-κB activation by KRAS requires the IKKβ kinase, and that targeting IKKβ reduces KRAS-induced lung tumor growth in vivo, but has limited effects on cell growth in vitro, we hypothesized that IKKβ targeting would reduce lung tumor growth by inhibiting KRAS-induced angiogenesis. Materials and methods: To test this hypothesis, we targeted IKKβ in KRAS-mutant lung cancer cell lines either by siRNA-mediated transfection or by treatment with Compound A (CmpdA), a highly specific IKKβ inhibitor, and used in vitro and in vivo assays to evaluate angiogenesis. Results and conclusions: Both pharmacological and siRNA-mediated IKKβ targeting in lung cells reduced expression and secretion of NF-κB-regulated proangiogenic factors IL-8 and VEGF. Moreover, conditioned media from IKKβ-targeted lung cells reduced human umbilical vein endothelial cell (HUVEC) migration, invasion and tube formation in vitro. Furthermore, siRNA-mediated IKKβ inhibition reduced xenograft tumor growth and vascularity in vivo. Finally, IKKβ inhibition also affects endothelial cell function in a cancer-independent manner, as IKKβ inhibition reduced pathological retinal angiogenesis in a mouse model of oxygen-induced retinopathy. Taken together, these results provide a novel mechanistic understanding of how the IKKβ pathway affects human lung tumorigenesis, indicating that IKKβ promotes KRAS-induced angiogenesis both by cancer cell-intrinsic and cancer cell-independent mechanisms, which strongly suggests IKKβ inhibition as a promising antiangiogenic approach to be explored for KRAS-induced lung cancer therapy
Leishmania amazonensis downregulates macrophage iNOS expression via Histone Deacetylase 1 (HDAC1): a novel parasite evasion mechanism.
The induced expression of nitric oxide synthase (iNOS) controls the intracellular growth of Leishmania in infected macrophages. Histones deacetylases (HDACs) negatively regulate gene expression through the formation of complexes containing transcription factors such as NF-κB p50/50. Herein, we demonstrated the occupancy of p50/p50_HDAC1 to iNOS promoter associated with reduced levels of H3K9Ac. Remarkably, we found increased levels of HDAC1 in L. amazonensis-infected macrophages. HDAC1 upregulation was not found in L. major-infected macrophages. The parasite intracellular load was reduced in HDAC1 knocked-down macrophages, which presented increased nitric oxide levels. HDAC1 silencing led to the occupancy of CBP/p300 to iNOS promoter and the rise of H3K9Ac modification. Importantly, the immunostaining of skin samples from hiporeactive cutaneous leishmaniasis patients infected with L. amazonensis, revealed high levels of HDAC1. In brief, L. amazonensis induces HDAC1 in infected macrophages, which contribute to parasite survival and is associated to hiporeactive stage found in L. amazonensis infected patients
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