Reef-scale assessment of intertidal large benthic foraminifera populations on one tree Island, great barrier reef and their future carbonate production potential in a warming ocean

Populations of large benthic foraminiferans (LBFs) that inhabit coral reef platforms are major producers of calcium carbonate (CaCO3) in reef ecosystems. This study documented the population density of living intertidal LBF populations at One Tree Reef (OTR), southern Great Barrier Reef, in a community dominated by Marginopora vertebralis and Baculogypsina sphaerulata. Densities of 7.7 × 103 M. vertebralis individuals (ind.)/m2 and 4.5 × 105 B. sphaerulata ind./m2 were estimated for these populations in May 2011. We applied remote-sensing technology to determine reef-scale estimates of suitable Foraminifera habitats and used these to estimate overall stocks of LBF populations on the intertidal algal flat at OTR of ca. 2800 metric tons. The growth rate of M. vertebralis was determined in a laboratory study, and the data were used to calculate the annual CaCO3 production of the reef flat by the LBF population. The response of M. vertebralis to ocean warming was investigated using 3-week incubations at temperatures ranging from ambient sea surface temperature to +6°C. There were significant decreases in growth and concomitant CaCO3 production in 6°C warmer water, which resulted in shell dissolution of M. vertebralis. These results indicate that climate-driven ocean warming projected for the region will result in significant decreases in CaCO3 production in overall foraminiferan populations, although species-specific effects should be further investigated

Amelioration of ocean acidification and warming effects through physiological buffering of a macroalgae

Concurrent anthropogenic global climate change and ocean acidification are expected to have a negative impact on calcifying marine organisms. While knowledge of biological responses of organisms to oceanic stress has emerged from single-species experiments, these do not capture ecologically relevant scenarios where the potential for multi-organism physiological interactions is assessed. Marine algae provide an interesting case study, as their photosynthetic activity elevates pH in the surrounding microenvironment, potentially buffering more acidic conditions for associated epiphytes. We present findings that indicate increased tolerance of an important epiphytic for aminifera, Marginopora vertebralis, to the effects of increased temperature (+/- 3 degrees C) and p CO2(similar to 1,000 mu atm) when associated with its common algal host, Laurencia intricata. Specimens of M. vertebralis were incubated for 15 days in flow-through aquaria simulating current and end-of-century temperature and pH conditions. Physiological measures of growth (change in wet weight), calcification (measured change in total alkalinity in closed bottles), photochemical efficiency (Fv/Fm), total chlorophyll, photosynthesis (oxygen flux), and respiration were determined. When incubated in isolation,M. vertebralis-exhibited reduced growth in end-of-century projections of ocean acidification conditions, while calcification rates were lowest in the high-temperature, low-pH treatment. Interestingly, association with L. intricata ameliorated these stress effects with the growth and calcification rates ofM. vertebralis being similar to those observed in ambient conditions. Total chlorophyll levels in M. vertebral is decreased when in association with L. intricata, while maximum photochemical efficiency increased in ambient conditions. Net production estimates remained similar between M. vertebralis in isolation and in association with L. intricata, although both production and respiration rates ofM. vertebralis were significantly higher when associated with L. intricata. These results indicate that the association with L. intricata increases the resilience ofM. vertebralis to climate change stress, providing one of the first examples of physiological buffering by a marine alga that can ameliorate the negative effects of changing ocean conditions

Global declines in coral reef calcium carbonate production under ocean acidification and warming

Ocean warming and acidification threaten the future growth of coral reefs. This is because the calcifying coral reef taxa that construct the calcium carbonate frameworks and cement the reef together are highly sensitive to ocean warming and acidification. However, the global-scale effects of ocean warming and acidification on rates of coral reef net carbonate production remain poorly constrained despite a wealth of studies assessing their effects on the calcification of individual organisms. Here, we present global estimates of projected future changes in coral reef net carbonate production under ocean warming and acidification. We apply a meta-analysis of responses of coral reef taxa calcification and bioerosion rates to predicted changes in coral cover driven by climate change to estimate the net carbonate production rates of 183 reefs worldwide by 2050 and 2100. We forecast mean global reef net carbonate production under representative concentration pathways (RCP) 2.6, 4.5, and 8.5 will decline by 76, 149, and 156%, respectively, by 2100. While 63% of reefs are projected to continue to accrete by 2100 under RCP2.6, 94% will be eroding by 2050 under RCP8.5, and no reefs will continue to accrete at rates matching projected sea level rise under RCP4.5 or 8.5 by 2100. Projected reduced coral cover due to bleaching events predominately drives these declines rather than the direct physiological impacts of ocean warming and acidification on calcification or bioerosion. Presently degraded reefs were also more sensitive in our analysis. These findings highlight the low likelihood that the world’s coral reefs will maintain their functional roles without near-term stabilization of atmospheric CO2 emissions

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase 1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age  6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score  652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc = 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N = 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in Asia and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

The Effect of Ocean Acidification on Skeletal Structures

It is well known that the increasing partial pressure of atmospheric CO2 (pCO2) is reducing surface ocean pH, a process known as ocean acidification (OA) [...

Ocean Warming Amplifies the Effects of Ocean Acidification on Skeletal Mineralogy and Microstructure in the Asterinid Starfish Aquilonastra yairi

Ocean acidification and ocean warming compromise the capacity of calcifying marine organisms to generate and maintain their skeletons. While many marine calcifying organisms precipitate low-Mg calcite or aragonite, the skeleton of echinoderms consists of more soluble Mg-calcite. To assess the impact of exposure to elevated temperature and increased pCO2 on the skeleton of echinoderms, in particular the mineralogy and microstructure, the starfish Aquilonastra yairi (Echinodermata: Asteroidea) was exposed for 90 days to simulated ocean warming (27 °C and 32 °C) and ocean acidification (455 µatm, 1052 µatm, 2066 µatm) conditions. The results indicate that temperature is the major factor controlling the skeletal Mg (Mg/Ca ratio and Mgnorm ratio), but not for skeletal Sr (Sr/Ca ratio and Srnorm ratio) and skeletal Ca (Canorm ratio) in A. yairi. Nevertheless, inter-individual variability in skeletal Sr and Ca ratios increased with higher temperature. Elevated pCO2 did not induce any statistically significant element alterations of the skeleton in all treatments over the incubation time, but increased pCO2 concentrations might possess an indirect effect on skeletal mineral ratio alteration. The influence of increased pCO2 was more relevant than that of increased temperature on skeletal microstructures. pCO2 as a sole stressor caused alterations on stereom structure and degradation on the skeletal structure of A. yairi, whereas temperature did not; however, skeletons exposed to elevated pCO2 and high temperature show a strongly altered skeleton structure compared to ambient temperature. These results indicate that ocean warming might exacerbate the skeletal maintaining mechanisms of the starfish in a high pCO2 environment and could potentially modify the morphology and functions of the starfish skeleton

Spatial and temporal variation in reef-scale carbonate storage of large benthic foraminifera: a case study on One Tree Reef

Large benthic foraminifera (LBFs) are a vital component of coral reef carbonate production, often overlooked due to their small size. These super-abundant calcifiers are crucial to reef calcification by generation of lagoon and beach sands. Reef-scale carbonate production by LBFs is not well understood, and seasonal fluctuations in this important process are largely unquantified. The biomass of five LBF species in their algal flat habitat was quantified in the austral winter (July 2013), spring (October 2013), and summer (February 2014) at One Tree Reef. WorldView-2 satellite images were used to characterize and create LBF habitat maps based on ground-referenced photographs of algal cover. Habitat maps and LBF biomass measurements were combined to estimate carbonate storage across the entire reef flat. Total carbonate storage of LBFs on the reef flat ranged from 270 tonnes (winter) to 380 tonnes (summer). Satellite images indicate that the habitat area used by LBFs ranged from 0.6 (winter) to 0.71 km2 (spring) of a total possible area of 0.96 km2. LBF biomass was highest in the winter when algal habitat area was lowest, but total carbonate storage was the highest in the summer, when algal habitat area was intermediate. Our data suggest that biomass measurements alone do not capture total abundance of LBF populations (carbonate storage), as the area of available habitat is variable. These results suggest LBF carbonate production studies that measure biomass in discrete locations and single time points fail to capture accurate reef-scale production by not incorporating estimates of the associated algal habitat. Reef-scale measurements in this study can be incorporated into carbonate production models to determine the role of LBFs in sedimentary landforms (lagoons, beaches, etc.). Based on previous models of entire reef metabolism, our estimates indicate that LBFs contribute approximately 3.9¿5.4% of reef carbonate budgets, a previously underappreciated carbon sink

Seawater carbonate chemistry and changes in coral reef community structure in response to year‑long incubations under contrasting pCO2 regimes

Coral reefs are threatened by ocean acidification (OA), which depresses net calcification of corals, calcified algae, and coral reef communities. These effects have been quantified for many organisms, but most experiments last weeks-to-months, and do not test for effects on community structure. Here, the effects of OA on back reef communities from Mo'orea, French Polynesia (17.492 S, 149.826 W), were tested from 12 November 2015 to 16 November 2016 in outdoor flumes maintained at mean pCO2 levels of 364 µatm, 564 µatm, 761 µatm, and 1067 µatm. The communities consisted of four corals and two calcified algae, with change in mass (Gnet, a combination of gross accretion and dissolution) and percent cover recorded monthly. For massive Porites and Montipora spp., Gnet differed among treatments, and at 1067 µatm (relative to ambient) was reduced and still positive; for Porolithon onkodes, all of which died, Gnet was negative at high pCO2, revealing dissolution (sample sizes were too small for analysis of Gnet for other taxa). Growth rates (% cover month−1) were unaffected by pCO2 for Montipora spp., P. rus, Pocillopora verrucosa, and Lithophyllum kotschyanum, but were depressed for massive Porites at 564 µatm. Multivariate community structure changed among seasons, and the variation under all elevated pCO2 treatments differed from that recorded at 364 µatm, and was greatest under 564 µatm and 761 µatm pCO2. Temporal variation in multivariate community structure could not be attributed solely to the effects of OA on the chemical and physical properties of seawater. Together, these results suggest that coral reef community structure may be more resilient to OA than suggested by the negative effects of high pCO2 on Gnet of their component organisms

Seawater carbonate chemistry and resistance to ocean acidification in coral reef taxa

Ocean acidification (OA) is a major threat to coral reefs, which are built by calcareous species. However, long-term assessments of the impacts of OA are scarce, limiting the understanding of the capacity of corals and coralline algae to acclimatize to high partial pressure of carbon dioxide (pCO2) levels. Species-specific sensitivities to OA are influenced by its impacts on chemistry within the calcifying fluid (CF). Here, we investigate the capacity of multiple coral and calcifying macroalgal species to acclimatize to elevated pCO2 by determining their chemistry in the CF during a year-long experiment. We found no evidence of acclimatization to elevated pCO2 across any of the tested taxa. The effects of increasing seawater pCO2 on the CF chemistry were rapid and persisted until the end of the experiment. Our results show that acclimatization of the CF chemistry does not occur within one year, which confirms the threat of OA for future reef accretion and ecological function

3D photogrammetry quantifies growth and external erosion of individual coral colonies and skeletons

Growth and contraction of ecosystem engineers, such as trees, influence ecosystem structure and function. On coral reefs, methods to measure small changes in the structure of microhabitats, driven by growth of coral colonies and contraction of skeletons, are extremely limited. We used 3D reconstructions to quantify changes in the external structure of coral colonies of tabular Acropora spp., the dominant habitat-forming corals in shallow exposed reefs across the Pacific. The volume and surface area of live colonies increased by 21% and 22%, respectively, in 12 months, corresponding to a mean annual linear extension of 5.62 cm yr−1 (±1.81 SE). The volume and surface area of dead skeletons decreased by 52% and 47%, respectively, corresponding to a mean decline in linear extension of −29.56 cm yr−1 (±7.08 SE), which accounted for both erosion and fragmentation of dead colonies. This is the first study to use 3D photogrammetry to assess fine-scale structural changes of entire individual colonies in situ, quantifying coral growth and contraction. The high-resolution of the technique allows for detection of changes on reef structure faster than other non-intrusive approaches. These results improve our capacity to measure the drivers underpinning ecosystem biodiversity, status and trajectory