Performance Simulation of Marine Cycloidal Propellers: A Both Theoretical and Heuristic Approach

open4The importance of mathematical and numerical simulation in marine engineering is growing together with the complexity of the designed systems. In general, simulation a makes it possible to improve the engineering design, reducing working time and costs of production as well. In this respect, the implementation of a simulation model for cycloidal propellers is presented. Cycloidal thrusters are being increasingly used in marine applications. Their best performance concerns low-speed applications, due to their ability to steer thrust in any direction. The proposed simulator is able to assess the performance of cycloidal propellers in terms of the generated thrust and torque, without resorting to consuming and demanding computational tools, such as CFD methods. This feature makes the presented model particularly suitable for the simulation in the time domain of the maneuverability of surface units, equipped with cycloidal propellers. In this regard, after embodying the implemented model in an already existing simulation platform for maneuverability, we show the most significant outputs concerning some simulated maneuvers, performed at cruise speed.openMarco Altosole, Silvia Donnarumma, Valentina Spagnolo, Stefano VignoloAltosole, Marco; Donnarumma, Silvia; Spagnolo, Valentina; Vignolo, Stefan

Antimicrobial Peptides Human Beta-Defensin-2 and -3 Protect the Gut During Candida albicans Infections Enhancing the Intestinal Barrier Integrity: In Vitro Study

The intestinal mucosa is composed of a monolayer of epithelial cells, which is highly polarized and firmly united to each other thanks to the presence of proteins complexes, called Tight junctions (TJs). Alteration of the mucus layer and TJs causes an increase in intestinal permeability, which can lead to a microbial translocation and systemic disorders. Candida albicans, in addition to its role of commensal, is an opportunistic pathogen responsible for disseminated candidiasis, especially in immunocompromised subjects where the dysbiosis leads to damage of the intestinal mucosal barrier. In this work, we used a line of intestinal epithelial cells able to stably express the genes that encodes human beta defensin-2 (HBD-2) and -3 (HBD-3) to monitor the invasion of C. albicans in vitro. Defensins are a group of antimicrobial peptides (AMPs) found in different living organisms, and are involved in the first line of defense in the innate immune response against pathogens. The results obtained show that the presence of antimicrobial peptides improves the expression of TJs and increases the Trans Epithelial Electrical Resistence value. In addition, the invasive ability of C. albicans in transfected cells is significantly reduced, as well as the expression levels of genes involved in the apoptotic pathway. Through the study of interaction between antimicrobial peptides and microbiota we will be able in the future to better understand the mechanisms by which they exert the host defense function against intestinal pathogens

Antimicrobial peptide human β-defensin-2 improves in vitro cellular viability and reduces pro-inflammatory effects induced by enteroinvasive Escherichia coli in Caco-2 cells by inhibiting invasion and virulence factors’ expression

Escherichia coli is one of the commensal species most represented in the intestinal microbiota. However, there are some strains that can acquire new virulence factors that enable them to adapt to new intestinal niches. These include enteroinvasive E. coli (EIEC) that is responsible for the bacillary dysentery that causes severe diarrheal symptoms in both children and adults. Due to the increasing onset of antibiotic resistance phenomena, scientific research is focused on the study of other therapeutic approaches for the treatment of bacterial infections. A promising alternative could be represented by antimicrobial peptides (AMPs), that have received widespread attention due to their broad antimicrobial spectrum and low incidence of bacterial resistance. AMPs modulate the immune defenses of the host and regulate the composition of microbiota and the renewal of the intestinal epithelium. With the aim to investigate an alternative therapeutic approach, especially in the case of antibiotic resistance, in this work we created a line of intestinal epithelial cells able to express high concentrations of AMP human β-defensin-2 (HBD-2) in order to test its ability to interfere with the pathogenicity mechanisms of EIEC. The results showed that HBD-2 is able to significantly reduce the expression of the proinflammatory cytokines by intestinal epithelial cells, the invasiveness ability of EIEC and the expression of invasion-associated genes

Langerhans' cell histiocytosis of the vulva: The use of photodynamic therapy

Background: The evaluation of possible efficacy of Photodynamic Therapy (PDT) in a rare condition of vulvar Langerhans' cell histiocytosis (LCH), in a 43-years-old women with one year history of pruritic vulvar lesions. Main observations: After a cycle of 5 sessions of PDT, with aminolevulinic acid (ALA) in low percentage (5%), the patient reported an improvement of lesions, and an high improvement of local symptoms, with a significant reduction of itch and pain. Conclusions: Our case confirms this single experience of literature for the treatment of vulvar LCH by PDT, with an improvement of disease's symptoms, and can suggest the use of PDT for this rare and untreated disease

Powerful high energy emission of the remarkable BL Lac object S5 0716+714

BL Lac objects of the intermediate subclass (IBLs) are known to emit a substantial fraction of their power in the energy range 0.1--10 GeV. Detecting gamma-ray emission from such sources provides therefore a direct probe of the emission mechanisms and of the underlying powerhouse. The AGILE gamma-ray satellite detected the remarkable IBL S5 0716+714 (z \simeq 0.3) during a high state in the period from 2007 September - October, marked by two very intense flares reaching peak fluxes of 200\times10^{-8} ph / cm^2 s above 100 MeV, with simultaneous optical and X-ray observations. We present here a theoretical model for the two major flares and discuss the overall energetics of the source. We conclude that 0716+714 is among the brightest BL Lac's ever detected at gamma-ray energies. Because of its high power and lack of signs for ongoing accretion or surrounding gas, the source is an ideal candidate to test the maximal power extractable from a rotating supermassive black hole via the pure Blandford-Znajek (BZ) mechanism. We find that during the 2007 gamma-ray flares our source approached or just exceeded the upper limit set by BZ for a black hole of mass 10^9 M_sunComment: 12 pages, 3 figure

Gamma-ray blazars: the view from AGILE

During the first 3 years of operation the Gamma-Ray Imaging Detector onboard the AGILE satellite detected several blazars in a high gamma-ray activity: 3C 279, 3C 454.3, PKS 1510-089, S5 0716+714, 3C 273, W Comae, Mrk 421, PKS 0537-441 and 4C +21.35. Thanks to the rapid dissemination of our alerts, we were able to obtain multiwavelength data from other observatories such as Spitzer, Swift, RXTE, Suzaku, INTEGRAL, MAGIC, VERITAS, and ARGO as well as radio-to-optical coverage by means of the GASP Project of the WEBT and the REM Telescope. This large multifrequency coverage gave us the opportunity to study the variability correlations between the emission at different frequencies and to obtain simultaneous spectral energy distributions of these sources from radio to gamma-ray energy bands, investigating the different mechanisms responsible for their emission and uncovering in some cases a more complex behaviour with respect to the standard models. We present a review of the most interesting AGILE results on these gamma-ray blazars and their multifrequency data.Comment: 25 pages, 10 figures, accepted for publication on Advances in Space Research. Talk presented at the 38th COSPAR Scientific Assembly (Bremen, Germany; July 18-25, 2010

On the Angular Resolution of the AGILE gamma-ray imaging detector

We present a study of the Angular Resolution of the AGILE gamma-ray imaging detector (GRID) that is operational in space since April 2007. The AGILE instrument is made of an array of 12 planes each equipped with a Tungsten converter and Silicon micros trip detectors and is sensitive in the energy range 50 MeV - 10 GeV. Among the space instruments devoted to gamma-ray astrophysics, AGILE uniquely exploits an analog readout system with dedicated electronics coupled with Silicon detectors. We show the results of Monte Carlo simulations carried out to reproduce the gamma-ray detection by the GRID, and we compare them to in-flight data. We use the Crab (pulsar + Nebula) system for discussion of real data performance, since its E^{-2} energy spectrum is representative of the majority of gamma-ray sources. For Crab-like spectrum sources, the GRID angular resolution (FWHM of ~4deg at 100 MeV; ~0.8deg at 1 GeV; ~0.9deg integrating the full energy band from 100 MeV to tens of GeV) is stable across a large field of view, being characterized by a flat response up to 30deg off-axis. A comparison of the angular resolution obtained by the two operational gamma-ray instruments, AGILE-GRID and Fermi-LAT, is interesting in view of future gamma-ray missions, that are currently under study. The two instruments exploit different detector configurations affecting the angular resolution: the former being optimized in the readout and track reconstruction especially in the low-energy band, the latter in terms of converter thickness and power consumption. We show that, despite these differences, the angular resolution of both instruments is very similar between 100 MeV and a few GeV.Comment: 19 pages, 8 figures, accepted for publication in Ap