Factors Influencing Persistence of White-footed Mice

We examined factors that potentially influenced persistence of the white-footed mouse (Peromyscus leucopus) during 1981 to 1988 at Konza Prairie Biological Station, Kansas. We predicted that both abiotic (e.g., precipitation and temperature) and biotic (e.g., availability of food and density of conspecifics) factors would influence persisten~e of individuals at the study site. Persistence of individual white-footed mice on the study site differed among years and seasons. White-footed mice that were first captured in summer or in autumn persisted longer than those first captured in spring. Young females (less than 20 g) had greater persistence than young males, whereas old males (greater than or equal to 25 g) had greater persistence than old females. Persistence of white-footed mice captured in summer, autumn, and spring was related to abundance of white-footed mice, to production of seeds by woody plants, and to precipitation during MarchMay, respectively. Ambient temperature had no influence on persistence. We suggest that biotic and abiotic factors that influence persistence of white-footed mice are local in scale and that they affect persistence differentially at different times of the year

Influence of Habitat on Distribution and Abundance of the Eastern Woodrat in Kansas

Anthropogenic modification of native woodlands and grasslands in the Great Plains has altered the abundance and distribution of many species of mammals. To study habitat effects on the eastern woodrat (Neotoma floridana), we surveyed nests of the eastern woodrat in woodlands, grasslands, and croplands along 77 km of secondary roads in three counties in north-central Kansas. All nests were located in woodlands ( \u3c 2 %of habitat), although grasslands and croplands constituted 36% and 62% of habitat surveyed, respectively. In our survey, nests were associated positively with shelterbelts (3.6 nests per 100 m of road edge) but not with shrub patches (1.1 nests per 100 m of road edge) or riparian woodlands (0.3 nests per 100 m of road edge). Consequently, we specifically censused nests in an additional 12 riparian woodlands and 12 shelterbelts. Nests of eastern woodrats were less dense in riparian woodlands (9.4 nests/ha) than in shelterbelts (55.5 nests/ha). Density of woodrat nests decreased as width of a wooded area increased. Further, nests per 100 m of length of woodland did not increase as the width of woodland increased. These patterns suggest that woodland edge, not woodland interior, is the primary factor in abundance of eastern woodrats in this region. Although the eastern woodrat has previously been considered a woodland species, our results suggest that this assessment is incorrect. Our observations demonstrate that anthropogenic modification of the Great Plains, in the form of planted shelterbelts and expanded riparian woodland, likely has increased the distribution and abundance of eastern woodrats, compared to the mid-1800s

Pest Flies of Pastured Cattle and Horses

NYS IPM Type: Livestock Fact SheetIntegrated pest management (IPM) of pest flies, midges, and mosquitoes of pastured cattle and Horses incorporates several key components: correct arthropod pest identification, monitoring, management action (i.e., treatment), and evaluation of the action. This fact sheet is intended to help the livestock producer identify arthropod pests and not to be a how-to document. For information on the other steps to successful pest management please consult one or more of the suggested readings or web sites provided in the references section

How Diverse Detrital Environments Influence Nutrient Stoichiometry Between Males and Females of the Co-Occurring Container Mosquitoes \u3ci\u3eAedes albopictus\u3c/i\u3e, \u3ci\u3eAe. Aegypti\u3c/i\u3e, and \u3ci\u3eCulex quinquefasciatus\u3c/i\u3e

Allocation patterns of carbon and nitrogen in animals are influenced by food quality and quantity, as well as by inherent metabolic and physiological constraints within organisms. Whole body stoichiometry also may vary between the sexes who differ in development rates and reproductive allocation patterns. In aquatic containers, such as tree holes and tires, detrital inputs, which vary in amounts of carbon and nitrogen, form the basis of the mosquito-dominated food web. Differences in development times and mass between male and female mosquitoes may be the result of different reproductive constraints, which could also influence patterns of nutrient allocation. We examined development time, survival, and adult mass for males and females of three co-occurring species, Aedes albopictus, Ae. aegypti, and Culex quinquefasciatus, across environments with different ratios of animal and leaf detritus. We quantified the contribution of detritus to biomass using stable isotope analysis and measured tissue carbon and nitrogen concentrations among species and between the sexes. Development times were shorter and adults were heavier for Aedes in animal versus leaf-only environments, whereas Culex development times were invariant across detritus types. Aedes displayed similar survival across detritus types whereas C. quinquefasciatus showed decreased survival with increasing leaf detritus. All species had lower values of 15N and 13C in leaf-only detritus compared to animal, however, Aedes generally had lower tissue nitrogen compared to C. quinquefasciatus. There were no differences in the C:N ratio between male and female Aedes, however, Aedes were different than C. quinquefasciatus adults, with male C. quinquefasciatus significantly higher than females. Culex quinquefasciatus was homeostatic across detrital environments. These results allow us to hypothesize an underlying stoichiometric explanation for the variation in performance of different container species under similar detrital environments, and if supported may assist in explaining the production of vector populations in nature

Individual and Combined Releases of Muscidifurax raptor and M. raptorellus (Hymenoptera: Pteromalidae) as a Biological Control Tactic Targeting House Flies in Dairy Calf Facilities

The impact of commercially reared house fly parasitoids released into nine dairy calf coverall facilities were evaluated over 3 yr. Individual and equally mixed ratios of the pteromalid parasitoids Muscidifurax raptor Girault and Saunders and M. raptorellus Kogan and Legner were released at a rate of 500 parasitoids per calf per week for 8 wk. Prerelease, release, and postrelease parasitism was monitored using nearly 100,000 sentinel house fly, Musca domestica L., pupae during the 3 yr study. In general, very few adult parasitoids were recovered during the prerelease period and on the no-release farms during any period. However, considerable numbers of M. raptor and M. raptorellus were recovered from sentinel pupae on respective release farms. As expected, the greatest successful parasitism occurred during release periods, with a drop during postrelease periods. High successful parasitism and uneclosed pupae on M. raptorellus release farms suggests that this parasitoid was aggressive in attacking hosts with progeny production at approximately four wasps per pupa. Solitary releases of M. raptor provided sentinel mortality between 31 and 38%, whereas sentinel mortality on M. raptorellus-release farms was double, at 59-80%. Using mixed releases of the two species, overall fly mortality was slightly lower than that observed on M. raptorellus-only farms. This study documents the advantage of releasing M. raptorellus rather than M. raptor on New York dairy calf facilities, as supported by higher parasitism rates and lower costs (35-75%) for purchase of these gregarious wasps, as 75-80% fewer parasitized pupae are needed to achieve similar adult parasitoid level

Reactivating Fetal Hemoglobin Expression in Human Adult Erythroblasts Through BCL11A Knockdown Using Targeted Endonucleases.

We examined the efficiency, specificity, and mutational signatures of zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 systems designed to target the gene encoding the transcriptional repressor BCL11A, in human K562 cells and human CD34+ progenitor cells. ZFNs and TALENs were delivered as in vitro transcribed mRNA through electroporation; CRISPR/Cas9 was codelivered by Cas9 mRNA with plasmid-encoded guideRNA (gRNA) (pU6.g1) or in vitro transcribed gRNA (gR.1). Analyses of efficacy revealed that for these specific reagents and the delivery methods used, the ZFNs gave rise to more allelic disruption in the targeted locus compared to the TALENs and CRISPR/Cas9, which was associated with increased levels of fetal hemoglobin in erythroid cells produced in vitro from nuclease-treated CD34+ cells. Genome-wide analysis to evaluate the specificity of the nucleases revealed high specificity of this specific ZFN to the target site, while specific TALENs and CRISPRs evaluated showed off-target cleavage activity. ZFN gene-edited CD34+ cells had the capacity to engraft in NOD-PrkdcSCID-IL2Rγnull mice, while retaining multi-lineage potential, in contrast to TALEN gene-edited CD34+ cells. CRISPR engraftment levels mirrored the increased relative plasmid-mediated toxicity of pU6.g1/Cas9 in hematopoietic stem/progenitor cells (HSPCs), highlighting the value for the further improvements of CRISPR/Cas9 delivery in primary human HSPCs

Biomass burning emission inventory with daily resolution: Application to aircraft observations of Asian outflow

Asian outflo

The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells.

Lentiviral vectors designed for the treatment of the hemoglobinopathies require the inclusion of regulatory and strong enhancer elements to achieve sufficient expression of the β-globin transgene. Despite the inclusion of these elements, the efficacy of these vectors may be limited by transgene silencing due to the genomic environment surrounding the integration site. Barrier insulators can be used to give more consistent expression and resist silencing even with lower vector copies. Here, the barrier activity of an insulator element from the human ankyrin-1 gene was analyzed in a lentiviral vector carrying an antisickling human β-globin gene. Inclusion of a single copy of the Ankyrin insulator did not affect viral titer, and improved the consistency of expression from the vector in murine erythroleukemia cells. The presence of the Ankyrin insulator element did not change transgene expression in human hematopoietic cells in short-term erythroid culture or in vivo in primary murine transplants. However, analysis in secondary recipients showed that the lentiviral vector with the Ankyrin element preserved transgene expression, whereas expression from the vector lacking the Ankyrin insulator decreased in secondary recipients. These studies demonstrate that the Ankyrin insulator may improve long-term β-globin expression in hematopoietic stem cells for gene therapy of hemoglobinopathies