In-Situ Biodegradation of Organic Groundwater Contaminants: Delineating the Effect of Physical and Biological Processes by Reactive-Transport Modeling

Monitored Natural Attenuation (MNA) has emerged as a well recognized approach for the remediation of BTEX contaminated aquifers. In order to deem MNA as an adequate remediation scheme, their must exist proof of the occurrence of biodegradation processes at the site of interest. Furthermore, a comprehensive understanding of the coupled physical (e.g., advective transport, diffusion, and sorption) and biological (microbially-catalyzed contaminant degradation) processes, acting on the compound(s) of interest, is required to judge the natural attenuation potential of a certain site. This thesis is organized in two main parts. The first part focuses on the effects of transverse dispersion and sorption on the stable-carbon isotope signature of organic contaminants, and its potential interference with the assessment of in-situ biodegradation by compound-specific isotope analysis (CSIA). I performed scenario simulations for fringe-controlled ethylbenzene degradation in steady-state contaminant plumes, and analyzed a toluene-pulse experiment performed in an indoor model aquifer via reactivetransport modeling. The results of these two studies indicate that physical processes may affect the isotope signature of organic contaminants in groundwater systems, by either acting as rate-limiting step for biodegradation (e.g., transverse mixing in fringecontrolled biodegradation) or fractionating themselves between isotopically light and heavy contaminant molecules (e.g., transverse dispersion and sorption). The second part of this thesis addresses the influence of transient environmental conditions, such as the temporal absence of electron donors and/or acceptors (which might arise, e.g., due to changing hydraulic conditions) on in-situ biodegradation. I analyzed toluene degradation experiments, performed under aerobic conditions in one and two-dimensional bench-scale porous flow-through systems, by reactive-transport modeling. The analysis indicated that temporal periods of starvation of up to four months, which were induced in the experiments by interrupting the injection of the growth-substrate toluene, did not drastically reduce the biodegradation potential. To capture the dynamics of the system, the numerical modeling approach necessitated the inclusion of microbial dormancy, i.e., the switch to an ’inactive’ state of low metabolic activity under unfavorable conditions, as well as peak cell detachment during growth of sessile bacteria

Operating System Concepts for Reconfigurable Computing: Review and Survey

One of the key future challenges for reconfigurable computing is to enable higher design productivity and a more easy way to use reconfigurable computing systems for users that are unfamiliar with the underlying concepts. One way of doing this is to provide standardization and abstraction, usually supported and enforced by an operating system. This article gives historical review and a summary on ideas and key concepts to include reconfigurable computing aspects in operating systems. The article also presents an overview on published and available operating systems targeting the area of reconfigurable computing. The purpose of this article is to identify and summarize common patterns among those systems that can be seen as de facto standard. Furthermore, open problems, not covered by these already available systems, are identified

On the electrolyte convection around a hydrogen bubble evolving at a microelectrode under the influence of a magnetic field

Water electrolysis was carried out in a 1 M H2SO4 solution under different potentiostatic conditions in the presence of a magnetic field oriented normal to the horizontal microelectrode (100 μm in diameter). The imposed magnetohydrodynamic (MHD) electrolyte flow around the evolving hydrogen bubble was studied to clarify the effect on the detachment of the bubble from the electrode and the mass transfer toward the electrode. Different particle imaging and tracking techniques were applied to measure the three-dimensional flow in the bulk of the cell as well as in close vicinity of the evolving bubble. The periodic bubble growth cycle was analyzed by measurements of the current oscillations and microscopic high-speed imaging. In addition, a numerical study of the flow was conducted to support the experimental results. The results demonstrate that the MHD flow imposes only a small stabilizing force on the bubble. However, the observed secondary flow enhances the mass transfer toward the electrode and may reduce the local supersaturation of dissolved hydrogen

Polymer-Enforced Crystallization of a Eutectic Binary Hard Sphere Mixture

We prepared a buoyancy matched binary mixture of polydisperse polystyrene microgel spheres of size ratio 0.785 and at a volume fraction of 0.567 just below the kinetic glass transition. In line with theoretical expectations, a eutectic phase behavior was observed, but only a minor fraction of the samples crystallized at all. By adding a short non-adsorbing polymer we enforce inter-species fractionation into coexisting pure component crystals, which in turn also shows signs of intra-species fractionation. We show that in formerly inaccessible regions of the phase diagram binary hard sphere physics is made observable using attractive hard spheres. Ancillary files: Correction to Soft Matter 2012, 8, 627Comment: 4 pages, 2 Figures, Final accepted draft plus correctio

Interactions of Adiponectin and Lipopolysaccharide from Porphyromonas gingivalis on Human Oral Epithelial Cells

BACKGROUND: Periodontitis is an inflammatory disease caused by pathogenic microorganisms, such as Porphyromonas gingivalis, and characterized by the destruction of the periodontium. Obese individuals have an increased risk for periodontitis and show decreased serum levels of adiponectin. This in-vitro study was established to examine whether adiponectin modulates critical effects of lipopolysaccharide (LPS) from P. gingivalis on oral epithelial cells (OECs). METHODOLOGY/PRINCIPAL FINDINGS: The presence of adiponectin and its receptors in human gingival tissue samples and OECs was analyzed by immunohistochemistry and PCR. Furthermore, OECs were treated with LPS and/or adiponectin for up to 72 h, and the gene expression and protein synthesis of pro- and anti-inflammatory mediators, matrix metalloproteinases (MMPs) and growth factors were analyzed by real-time PCR and ELISA. Additionally, cell proliferation, differentiation and in-vitro wound healing were studied. The nuclear translocation of NFκB was investigated by immunofluorescence. Gingival tissue sections showed a strong synthesis of adiponectin and its receptors in the epithelial layer. In cell cultures, LPS induced a significant up-regulation of interleukin (IL) 1β, IL6, IL8, MMP1 and MMP3. Adiponectin abrogated significantly the stimulatory effects of LPS on these molecules. Similarly, adiponectin inhibited significantly the LPS-induced decrease in cell viability and increase in cell proliferation and differentiation. Adiponectin led to a time-dependent induction of the anti-inflammatory mediators IL10 and heme oxygenase 1, and blocked the LPS-stimulated NFκB nuclear translocation. CONCLUSIONS/SIGNIFICANCE: Adiponectin may counteract critical actions of P. gingivalis on oral epithelial cells. Low levels of adiponectin, as observed in obese individuals, may increase the risk for periodontal inflammation and destruction

Multiwavelength study of the galactic PeVatron candidate LHAASO J2108+5157

Context. Several new ultrahigh-energy (UHE) γ-ray sources have recently been discovered by the Large High Altitude Air Shower Observatory (LHAASO) collaboration. These represent a step forward in the search for the so-called Galactic PeVatrons, the enigmatic sources of the Galactic cosmic rays up to PeV energies. However, it has been shown that multi-TeV γ-ray emission does not necessarily prove the existence of a hadronic accelerator in the source; indeed this emission could also be explained as inverse Compton scattering from electrons in a radiation-dominated environment. A clear distinction between the two major emission mechanisms would only be made possible by taking into account multi-wavelength data and detailed morphology of the source. Aims. We aim to understand the nature of the unidentified source LHAASO J2108+5157, which is one of the few known UHE sources with no very high-energy (VHE) counterpart. Methods. We observed LHAASO J2108+5157 in the X-ray band with XMM-Newton in 2021 for a total of 3.8 hours and at TeV energies with the Large-Sized Telescope prototype (LST-1), yielding 49 hours of good-quality data. In addition, we analyzed 12 years of Fermi-LAT data, to better constrain emission of its high-energy (HE) counterpart 4FGL J2108.0+5155. We used naima and jetset software packages to examine the leptonic and hadronic scenario of the multi-wavelength emission of the source. Results. We found an excess (3.7σ) in the LST-1 data at energies E > 3 TeV. Further analysis of the whole LST-1 energy range, assuming a point-like source, resulted in a hint (2.2σ) of hard emission, which can be described with a single power law with a photon index of Σ = 1.6 ± 0.2 the range of 0.3 - 100 TeV. We did not find any significant extended emission that could be related to a supernova remnant (SNR) or pulsar wind nebula (PWN) in the XMM-Newton data, which puts strong constraints on possible synchrotron emission of relativistic electrons. We revealed a new potential hard source in Fermi-LAT data with a significance of 4σ and a photon index of Σ = 1.9 ± 0.2, which is not spatially correlated with LHAASO J2108+5157, but including it in the source model we were able to improve spectral representation of the HE counterpart 4FGL J2108.0+5155. Conclusions. The LST-1 and LHAASO observations can be explained as inverse Compton-dominated leptonic emission of relativistic electrons with a cutoff energy of 100-30+70 TeV. The low magnetic field in the source imposed by the X-ray upper limits on synchrotron emission is compatible with a hypothesis of a PWN or a TeV halo. Furthermore, the spectral properties of the HE counterpart are consistent with a Geminga-like pulsar, which would be able to power the VHE-UHE emission. Nevertheless, the lack of a pulsar in the neighborhood of the UHE source is a challenge to the PWN/TeV-halo scenario. The UHE γ rays can also be explained as π0 decay-dominated hadronic emission due to interaction of relativistic protons with one of the two known molecular clouds in the direction of the source. Indeed, the hard spectrum in the LST-1 band is compatible with protons escaping a shock around a middle-aged SNR because of their high low-energy cut-off, but the origin of the HE γ-ray emission remains an open question