Individual limb mechanical analysis of gait following stroke

The step-to-step transition of walking requires significant mechanical and metabolic energy to redirect the center of mass. Inter-limb mechanical asymmetries during the step-to-step transition may increase overall energy demands and require compensation during single-support. The purpose of this study was to compare individual limb mechanical gait asymmetries during the step-to-step transitions, single-support and over a complete stride between two groups of individuals following stroke stratified by gait speed (≥0.8 m/s o

DOES PRE-EXERCISE STRETCHING AFFECT MUSCLE TENDON UNIT COMPLIANCE?

INTRODUCTION: Pre-exercise stretching is carried out by the majority of recreational and professional athletes, yet there is conflicting evidence with regard to the benefits of such programmes to either performance or injury risk reduction. It is unclear whether pre-exercise stretching can affect compliance, and at what level of the muscle-tendon unit (MTU) this effect may occur. Therefore the aim of this study was to determine whether an active preexercise stretching protocol increased the compliance of the triceps surae muscle-tendon complex and whether changes in compliance occurred in the muscle body or free tendon

Enhanced Proteolysis of β-Amyloid in APP Transgenic Mice Prevents Plaque Formation, Secondary Pathology, and Premature Death

AbstractConverging evidence suggests that the accumulation of cerebral amyloid β-protein (Aβ) in Alzheimer's disease (AD) reflects an imbalance between the production and degradation of this self-aggregating peptide. Upregulation of proteases that degrade Aβ thus represents a novel therapeutic approach to lowering steady-state Aβ levels, but the consequences of sustained upregulation in vivo have not been studied. Here we show that transgenic overexpression of insulin-degrading enzyme (IDE) or neprilysin (NEP) in neurons significantly reduces brain Aβ levels, retards or completely prevents amyloid plaque formation and its associated cytopathology, and rescues the premature lethality present in amyloid precursor protein (APP) transgenic mice. Our findings demonstrate that chronic upregulation of Aβ-degrading proteases represents an efficacious therapeutic approach to combating Alzheimer-type pathology in vivo

The effect of muscle-tendon unit vs. fascicle analyses on vastus lateralis force-generating capacity during constant power output cycling with variable cadence

The maximum force-generating capacity of a muscle is dependent on the lengths and velocities of its contractile apparatus. Muscle-tendon unit (MTU) length changes can be estimated from joint kinematics; however, contractile element length changes are more difficult to predict during dynamic contractions. The aim of this study was to compare vastus lateralis (VL) MTU and fascicle level force-length and force-velocity relationships, and dynamic muscle function while cycling at a constant submaximal power output (2.5 W/kg) with different cadences. We hypothesized that manipulating cadence at a constant power output would not affect VL MTU shortening, but significantly affect VL fascicle shortening. Furthermore, these differences would affect the predicted force capacity of the muscle. Using an isokinetic dynamometer and B-mode ultrasound (US), we determined the force-length and force-velocity properties of the VL MTU and its fascicles. In addition, three-dimensional kinematics and kinetics of the lower limb, as well as US images of VL fascicles were collected during submaximal cycling at cadences of 40, 60, 80, and 100 rotations per minute. Ultrasound measures revealed a significant increase in fascicle shortening as cadence decreased (84% increase across all conditions, P < 0.01), whereas there were no significant differences in MTU lengths across any of the cycling conditions (maximum of 6%). The MTU analysis resulted in greater predicted force capacity across all conditions relative to the force-velocity relationship (P < 0.01). These results reinforce the need to determine muscle mechanics in terms of separate contractile element and connective tissue length changes during isokinetic contractions, as well as dynamic movements like cycling

In vivo fascicle length measurements via B-mode ultrasound imaging with single vs dual transducer arrangements

Ultrasonography is a useful technique to study muscle contractions in vivo, however larger muscles like vastus lateralis may be difficult to visualise with smaller, commonly used transducers. Fascicle length is often estimated using linear trigonometry to extrapolate fascicle length to regions where the fascicle is not visible. However, this approach has not been compared to measurements made with a larger field of view for dynamic muscle contractions. Here we compared two different single-transducer extrapolation methods to measure VL muscle fascicle length to a direct measurement made using two synchronised, in-series transducers. The first method used pennation angle and muscle thickness to extrapolate fascicle length outside the image (extrapolate method). The second method determined fascicle length based on the extrapolated intercept between a fascicle and the aponeurosis (intercept method). Nine participants performed maximal effort, isometric, knee extension contractions on a dynamometer at 10° increments from 50 to 100° of knee flexion. Fascicle length and torque were simultaneously recorded for offline analysis. The dual transducer method showed similar patterns of fascicle length change (overall mean coefficient of multiple correlation was 0.76 and 0.71 compared to extrapolate and intercept methods respectively), but reached different absolute lengths during the contractions. This had the effect of producing force–length curves of the same shape, but each curve was shifted in terms of absolute length. We concluded that dual transducers are beneficial for studies that examine absolute fascicle lengths, whereas either of the single transducer methods may produce similar results for normalised length changes, and repeated measures experimental designs

Data from: The foot is more than a spring: human foot muscles perform work to adapt to the energetic requirements of locomotion

The foot has been considered both as an elastic mechanism that increases the efficiency of locomotion by recycling energy, as well as an energy sink that helps stabilize movement by dissipating energy through contact with the ground. We measured the activity of two intrinsic foot muscles, Flexor Digitorum Brevis (FDB) and Abductor Hallucis (AH), as well as the mechanical work performed by the foot as a whole and at a modelled plantar muscle tendon unit (MTU) to test whether these passive mechanics are actively controlled during stepping. We found that the underlying passive visco-elasticity of the foot is modulated by the muscles of the foot, facilitating both dissipation and generation of energy depending on the mechanical requirements at the center of mass (COM). Compared to level-ground stepping, the foot dissipated and generated an additional –0.2 J/kg and 0.10 J/kg (both P < 0.001) when stepping down and up a 26 cm step respectively, corresponding to 21 % and 10 % of the additional net work performed by the leg on the COM. Of this compensation at the foot, the plantar MTU performed 30 % and 89 % the work for step downs and step ups respectively. This work occurred early in stance and late in stance for stepping down respectively, when the activation levels of FDB and AH were increased between 69 % - 410 % compared to level steps (all P < 0.001). These findings suggest that the energetic function of the foot is actively modulated by the intrinsic foot muscles and may play a significant role in movements requiring large changes in net energy such as stepping on stairs or inclines, accelerating, decelerating, and jumping

Joint and muscle-tendon coordination strategies during submaximal jumping

Previous research has demonstrated that during submaximal jumping humans prioritize reducing energy consumption by minimizing countermovement depth. However, sometimes movement is constrained to a nonpreferred pattern, and this requires adaptation of neural control that accounts for complex interactions between muscle architecture, muscle properties, and task demands. This study compared submaximal jumping with either a preferred or a deep countermovement depth to examine how joint and muscle mechanics are integrated into the adaptation of coordination strategies in the deep condition. Three-dimensional motion capture, two force plates, electromyography, and ultrasonography were used to examine changes in joint kinetics and kinematics, muscle activation, and muscle kinematics for the lateral gastrocnemius and soleus. Results demonstrated that a decrease in ankle joint work during the deep countermovement depth was due to increased knee flexion, leading to unfavorably short biarticular muscle lengths and reduced active fascicle length change during ankle plantar flexion. Therefore, ankle joint work was likely decreased because of reduced active fascicle length change and operating position on the force-length relationship. Hip joint work was significantly increased as a result of altered muscle activation strategies, likely due to a substantially greater hip extensor muscle activation period compared with plantar flexor muscles during jumping. Therefore, coordination strategies at individual joints are likely influenced by time availability, where a short plantar flexor activation time results in dependence on muscle properties, instead of simply altering muscle activation, while the longer time for contraction of muscles at the hip allows for adjustments to voluntary neural control. Using human jumping as a model, we show that adapting movement patterns to altered task demands is achieved differently by muscles across the leg. Because of proximal-to-distal sequencing, distal muscles (i.e., plantar flexors) have reduced activation periods and, as a result, rely on muscle contractile properties (force-length relationship) for adjusting joint kinetics. For proximal muscles that have greater time availability, voluntary activation is modulated to adjust muscle outputs

The energetic behaviour of the human foot across a range of running speeds

The human foot contains passive elastic tissues that have spring-like qualities, storing and returning mechanical energy and other tissues that behave as dampers, dissipating energy. Additionally the intrinsic and extrinsic foot muscles have the capacity to act as dampers and motors, dissipating and generating mechanical energy. It remains unknown as to how the contribution of all passive and active tissues combine to produce the overall energetic function of the foot during running. Therefore, the aim of this study was to determine if the foot behaves globally as an active spring-damper during running. Fourteen participants ran on a force-instrumented treadmill at 2.2 ms, 3.3 ms and 4.4 ms, while foot segment motion was collected simultaneously with kinetic measurements. A unified deformable segment model was applied to quantify the instantaneous power of the foot segment during ground contact and mechanical work was calculated by integrating the foot power data. At all running speeds, the foot absorbed energy from early stance through to mid-stance and subsequently returned/generated a proportion of this energy in late stance. The magnitude of negative work performed increased with running speed, while the magnitude of positive work remained relatively constant across all running speeds. The proportion of energy dissipated relative to that absorbed (foot dissipation-ratio) was always greater than zero and increased with running speed, suggesting that the foot behaves as a viscous spring-damper

Supplement 1. Individual participant time normalised data for ankle, knee and hip joint angles and moments during all experimental conditions from Modulation of leg joint function to produce emulated acceleration during walking and running in humans

Understanding how humans adapt gait mechanics for a wide variety of locomotor tasks is important for inspiring the design of robotic, prosthetic and wearable assistive devices. We aimed to elicit the mechanical adjustments made to leg joint functions that are required to generate accelerative walking and running, using metrics with direct relevance to device design. Twelve healthy male participants completed constant speed (CS) walking and running and emulated acceleration (ACC) trials on an instrumented treadmill. External force and motion capture data were combined in an inverse dynamics analysis. Ankle, knee and hip joint mechanics were described and compared using angles, moments, powers and normalized functional indexes that described each joint as relatively more: spring, motor, damper or strut-like. To accelerate using a walking gait, the ankle joint was switched from predominantly spring-like to motor-like, while the hip joint was maintained as a motor, with an increase in hip motor-like function. Accelerating while running involved no change in the primary function of any leg joint, but involved high levels of spring and motor-like function at the hip and ankle joints. Mechanical adjustments for ACC walking were achieved primarily via altered limb positioning, but ACC running needed greater joint moments