Initial-boundary value problems for anisotropic elliptic-parabolic-pseudoparabolic equations with variable exponents of nonlinearity

Existence and uniqueness of weak solutions of initialboundary value problems for anisotropic elliptic-parabolic-pseudoparabolic equations with variable exponents of nonlinearity are proved. Estimates of the weak solutions of this problems are received. This estimates implies continuous dependence on the input data for the weak solutions of considered problem

How much do adults sit? Result from the German Health Update (GEDA 2019/2020-EHIS)

Background: Sedentary behaviour is increasingly perceived as a risk factor for the development of diseases and for increased mortality. In particular, increased time spent sitting in combination with low physical activity seems to have negative health consequences. Methods: In the nationwide cross-sectional study German Health Update (GEDA 2019/2020-EHIS), the indicator ‘sitting’ was captured by the self-report of the participants. Results: For at least eight hours a day, 16.7% of women and 22.3% of men sit: Men more often than women, younger persons more often than older persons and the proportion increases significantly from the low to the high education group. Similarly, about one fifth of adults in Germany sit for at least four hours a day and do not engage in physical activity in their leisure time. Conclusion: The results indicate that preventive measures are needed to reduce time spent sitting and increase physical activit

Hemagglutinin Spatial Distribution Shifts in Response to Cholesterol in the Influenza Viral Envelope

AbstractInfluenza virus delivers its genome to the host cytoplasm via a process of membrane fusion mediated by the viral hemagglutinin protein. Optimal fusion likely requires multiple hemagglutinin trimers, so the spatial distribution of hemagglutinin on the viral envelope may influence fusion mechanism. We have previously shown that moderate depletion of cholesterol from the influenza viral envelope accelerates fusion kinetics even though it decreases fusion efficiency, both in a reversible manner. Here, we use electron cryo-microscopy to measure how the hemagglutinin lateral density in the viral envelope changes with cholesterol extraction. We extract this information by measuring the radial distribution function of electron density in >4000 viral images per sample, assigning hemagglutinin density by comparing images with and without anti-HA Fab bound. On average, hemagglutinin trimers move closer together: we estimate that the typical trimer-trimer spacing reduces from 94 to 84 Å when ∼90% of cholesterol is removed from the viral membrane. Upon restoration of viral envelope cholesterol, this spacing once again expands. This finding can qualitatively explain the observed changes to fusion kinetics: contemporary models from single-virus microscopy are that fusion requires the engagement of several hemagglutinin trimers in close proximity. If removing cholesterol increases the lateral density of hemagglutinin, this should result in an increase in the rate of fusion

Wie viel sitzen Erwachsene? Ergebnisse der Studie Gesundheit in Deutschland aktuell (GEDA 2019/2020-EHIS)

Hintergrund: Sitzendes Verhalten wird zunehmend als ein Risikofaktor für die Entstehung von Erkrankungen und für eine erhöhte Sterblichkeit wahrgenommen. Insbesondere erhöhte Sitzzeiten in Kombination mit geringer körperlicher Aktivität scheinen negative Gesundheitsfolgen zu haben. Methode: In der bundesweiten Querschnittstudie Gesundheit in Deutschland aktuell (GEDA 2019/2020-EHIS) wurde der Indikator „Sitzen“ durch die Selbstangabe der Teilnehmenden erfasst. Ergebnisse: Für mindestens acht Stunden am Tag sitzen 16,7 % der Frauen und 22,3 % der Männer: Männer häufiger als Frauen, Jüngere häufiger als Ältere und der Anteil nimmt von der unteren zur oberen Bildungsgruppe deutlich zu. Ebenso sitzt etwa ein Fünftel der Erwachsenen in Deutschland mindestens vier Stunden am Tag und übt keine körperliche Aktivität in der Freizeit aus. Schlussfolgerung: Die Ergebnisse weisen darauf hin, dass präventive Maßnahmen notwendig sind, um Sitzzeiten zu reduzieren und körperliche Aktivität zu steiger

Solutions of ionic liquids with diverse aliphatic and aromatic solutes – Phase behavior and potentials for applications:A review article

This article principally reviews our research related to liquid–liquid and solid–liquid phase behavior of imidazolium- and phosphonium-based ionic liquids, mainly having bistriflamide ([NTf2]−) or triflate ([OTf]−) anions, with several aliphatic and aromatic solutes (target molecules). The latter include: (i) diols and triols: 1,2-propanediol, 1,3-propanediol and glycerol; (ii) polymer poly(ethylene glycol) (PEG): average molecular mass 200, 400 and 2050 – PEG200 (liquid), PEG400 (liquid) and PEG2050 (solid), respectively; (iii) polar aromatic compounds: nicotine, aniline, phenolic acids (vanillic, ferulic and caffeic acid,), thymol and caffeine and (iv) non-polar aromatic compounds (benzene, toluene, p-xylene). In these studies, the effects of the cation and anion, cation alkyl chain and PEG chain lengths on the observed phase behaviors were scrutinized. Thus, one of the major observations is that the anion – bistriflamide/triflate – selection usually had strong, sometimes really remarkable effects on the solvent abilities of the studied ionic liquids. Namely, in the case of the hydrogen-bonding solutes, the ionic liquids with the triflate anion generally exhibited substantially higher solubility than those having the bistriflamide anion. Nevertheless, with the aromatic compounds the situation was the opposite – in most of the cases it was the bistriflamide anion that favoured solubility. Moreover, our other studies confirmed the ability of PEG to dissolve both polar and non-polar aromatic compounds. Therefore, two general possibilities of application of alternative, environmentally acceptable, solvents of tuneable solvent properties appeared. One is to use homogeneous mixtures of two ionic liquids having [NTf2]− and [OTf]− anions as mixed solvents. The other, however, envisages the application of homogeneous and heterogeneous (PEG + ionic liquid) solutions as tuneable solvents for aromatic solutes. Such mixed solvents have potential applications in separation of the aforesaid target molecules from their aqueous solutions or in extraction from original matrices. From the fundamental point of view the phase equilibrium studies reviewed herein and the diversity of the pure compounds – ionic liquids and target molecules – represent a good base for the discussion of interactions between the molecules that exist in the studied solutions

Prevalence of Listeria species in camel sausages from retail markets in Aydin province in Turkey and RAPD analysis of Listeria monocytogenes isolates

Samples were taken from 100 camel sausages from the different retail markets in Aydin province in the south-west of Turkey and they were tested for the presence of Listeria spp by biochemical methods. Samples were enriched using Listeria Enrichment Broth and they were inoculated onto Listeria Selective Agar. Listeria monocytogenes was isolated from nine samples (9%), Listeria innocua from 14 samples (14%) and Listeria welshimeri from two samples(2%). A 701 bp fragment of listeriolysin O sequence for L. monocytogenes was amplified using specific primers by polymerase chain reaction (PCR) for confirmation of the identification. A random primer (OPA-11) was used in a random amplified polymorphic DNA (RAPD) assay. This detected five different band profiles amongst the L. monocytogenes isolates, indicating a relatively large amount of genetic heterogeneity amongst the nine isolates. The study has highlighted the need for improved strategies for food safety, in particular appropriate hygienic precautions to avoid contamination of sausage during the manufacturing process and appropriate preservation techniques during storage and transport, to prevent transmission of Listeria spp to consumers at home and abroad