Temperature and vernalisation regulation of seed properties in Brassica napus

As the global climate changes and temperatures increase in coming decades, crop yields are likely to be threatened. This includes in the UK, where warmer winters and summer heatwaves are expected to become more common. To ensure food production remains stable, it will be necessary to breed crops which can withstand these changing temperatures. In Brassica napus, seed properties including seed size, the number of seeds produced, and percentage of seed oil are all factors contributing to the yield. It is known that yield stability in Brassica napus is impacted by temperature during seed development and vernalisation. The effects of temperature on seed properties have been seen in Arabidopsis thaliana. Studies in Brassica napus have examined the genetic basis of yield separately to investigating how yield changes under different temperatures. However, the genetic basis of differences in how yield is affected by temperature has not yet been explored in-depth in Brassica napus. This thesis uses phenotypic approaches, transcriptome profiling and associative transcriptomics to describe the effect of vernalisation temperature, as well as temperature during seed set and seed maturation, on seed traits and gene expression in Brassica napus. This work reports a decrease in seed weight and seed number per pod associated with increasing seed maturation temperature from 18°C to 24°C, as well as with increasing vernalisation temperature from 5°C to 15°C. These results indicate that warmer temperatures lead to reductions in yield-related traits, particularly in Winter OSR. The decrease in mature seed size of Winter OSR resulting from an increased vernalisation temperature of 15°C were found to arise during a developmental window between 14 and 21 days after pollination. Associated changes in gene expression were described, including reduced expression of FLC genes in seeds produced by plants vernalised at 15°C compared to 5°C

Advances in conservation science and practice in Oceania: delivering on research priorities for the region

[Extract] Oceania is a diverse region both biologically and culturally. Yet the region is also marked by high rates of biodiversity loss due to major threats such as habitat destruction, climate change and invasive species (Kingsford et al., 2009). Evidence-based strategies are needed for successful conservation in the region, and targeted research delivering on urgent practical questions can support this. In 2015, we set out to identify research questions that, if answered, would increase the effectiveness of conservation and natural resource management practice and policy within Oceania in the next 10 years (Weeks & Adams, 2017). The priority questions that we identified emphasized the need for research that addresses distinctive management challenges prevalent in Oceania, and which fits the sociocultural contexts of the region. Five years on, we reflect on some of the progress made in answering these priority questions, with the eight papers featured in this Special Section addressing 12 of the 38 priority questions (Table 1)

Poly-ε-Lysine or Mel4 Antimicrobial Surface Modification on a Novel Peptide Hydrogel Bandage Contact Lens

Microbial keratitis (MK) is a serious issue in many countries and is often caused by contact lens wear. Antimicrobial peptides (AMPs) are a potentially useful tool for creating antimicrobial surfaces in light of increasing antibiotic resistance. Poly-ε-lysine (pεK) is an AMP that has been used extensively as a food preservative and Mel4 has recently been synthesized and studied as an antimicrobial coating for contact lenses. A hydrogel synthesized of pεK cross-linked with biscarboxylic acids provides a potential lens material which has many surface free amines, that can be subsequently used to attach additional AMPs, creating an antimicrobial lens. The aim of this study is to investigate pεK hydrogels against a clinical strain of Pseudomonas aeruginosa (P. aeruginosa) for preventing or treating MK. Covalent attachment of AMPs is investigated and confirmed by fluorescently tagged peptides. Bound pεK effectively reduces the number of adherent P. aeruginosa in vitro (>3 log). In ex vivo studies positive antimicrobial activity is observed on bare pεK hydrogels and those with additionally bound pεK or Mel4; lenses allow the maintenance of the corneal epithelium. A pεK hydrogel contact lens with additional AMPs can be a therapeutic tool to reduce the incidence of MK

Exploring variation in the use of feedback from national clinical audits : a realist investigation

BACKGROUND: National Clinical Audits (NCAs) are a well-established quality improvement strategy used in healthcare settings. Significant resources, including clinicians' time, are invested in participating in NCAs, yet there is variation in the extent to which the resulting feedback stimulates quality improvement. The aim of this study was to explore the reasons behind this variation. METHODS: We used realist evaluation to interrogate how context shapes the mechanisms through which NCAs work (or not) to stimulate quality improvement. Fifty-four interviews were conducted with doctors, nurses, audit clerks and other staff working with NCAs across five healthcare providers in England. In line with realist principles we scrutinised the data to identify how and why providers responded to NCA feedback (mechanisms), the circumstances that supported or constrained provider responses (context), and what happened as a result of the interactions between mechanisms and context (outcomes). We summarised our findings as Context+Mechanism = Outcome configurations. RESULTS: We identified five mechanisms that explained provider interactions with NCA feedback: reputation, professionalism, competition, incentives, and professional development. Professionalism and incentives underpinned most frequent interaction with feedback, providing opportunities to stimulate quality improvement. Feedback was used routinely in these ways where it was generated from data stored in local databases before upload to NCA suppliers. Local databases enabled staff to access data easily, customise feedback and, importantly, the data were trusted as accurate, due to the skills and experience of staff supporting audit participation. Feedback produced by NCA suppliers, which included national comparator data, was used in a more limited capacity across providers. Challenges accessing supplier data in a timely way and concerns about the quality of data submitted across providers were reported to constrain use of this mode of feedback. CONCLUSION: The findings suggest that there are a number of mechanisms that underpin healthcare providers' interactions with NCA feedback. However, there is variation in the mode, frequency and impact of these interactions. Feedback was used most routinely, providing opportunities to stimulate quality improvement, within clinical services resourced to collect accurate data and to maintain local databases from which feedback could be customised for the needs of the service

In fateful moments: the appeal of parent testimonials when selling private tutoring

Australia’s private tutoring market is expanding in a context where parents’ trust in school personnel as educational experts is vulnerable. Simultaneously, a parentocratic logic is nudging parents to infuse the resources at their disposal into their pedagogic work in order to achieve the educational outcomes that they wish for their children. However, little is known about the specific strategies that private tutoring suppliers are using to attract prospective parents. This paper reports on a study of 160 parent testimonials published on the websites of 16 private tutoring suppliers in Australia. Drawing on Giddens’ concept of ‘fateful moments’ and Bauman’s work on the persuasion of the peer example, together with tools of critical discourse analysis, we argue that parent testimonials use emotional appeals to construct private tutoring as a resolution to parents’ fateful moments. In addition, we speculate that this emotional footing resonates with the pedagogic preferences of the dominant Australian middle classes

Preoperative botulinum neurotoxin A for children with bilateral cerebral palsy undergoing major hip surgery: a randomized double-blind placebo-controlled trial.

AIM: To assess whether preoperative botulinum neurotoxin A (BoNT-A) affects pain after major hip surgery for children with bilateral cerebral palsy (CP). METHOD: This was a randomized, parallel arms, placebo-contolled trial. Children with hypertonic CP aged 2 to 15 years awaiting bony hip surgery at a tertiary hospital were randomized to receive either BoNT-A or placebo injections into the muscles of the hip on a single occasion immediately before surgery. The primary outcome was the paediatric pain profile (PPP), which was assessed at baseline and weekly for 6 weeks. Treatment allocation was by minimization. Participants, clinicians, and outcome assessors were masked to group assignment. RESULTS: Twenty-seven participants (17 males, 10 females; mean 8y 8mo [SD 3y 9mo], range 3y 4mo-15y 10mo) were allocated to BoNT-A and 27 participants (14 males, 13 females; mean 8y 11mo [SD 3y 5mo], range 4y 1mo-15y 2mo) to placebo. Mean (SD) PPP at 6 weeks for the BoNT-A group (n=24 followed up) was 10.96 (7.22) and for the placebo group (n=26) was 10.04 (8.54) (p=0.69; 95% confidence interval [CI] -4.82, 3.18). There were 16 serious adverse events in total during 6 months of follow-up (n=6 in BoNT-A group). INTERPRETATION: Use of BoNT-A immediately before bony hip surgery for reducing postoperative pain for children with CP was not supported. WHAT THIS PAPER ADDS: Botulinum neurotoxin A (BoNT-A) does not reduce postoperative pain following bony hip surgery. BoNT-A also does not affect postoperative quality of life

Institutional use of National Clinical Audits by healthcare providers

Rationale, aims, and objectives Healthcare systems worldwide devote significant resources towards collecting data to support care quality assurance and improvement. In the United Kingdom, National Clinical Audits are intended to contribute to these objectives by providing public reports of data on healthcare treatment and outcomes, but their potential for quality improvement in particular is not realized fully among healthcare providers. Here, we aim to explore this outcome from the perspective of hospital boards and their quality committees: an under‐studied area, given the emphasis in previous research on the audits' use by clinical teams. Methods We carried out semi‐structured, qualitative interviews with 54 staff in different clinical and management settings in five English National Health Service hospitals about their use of NCA data, and the circumstances that supported or constrained such use. We used Framework Analysis to identify themes within their responses. Results We found that members and officers of hospitals' governing bodies perceived an imbalance between the benefits to their institutions from National Clinical Audits and the substantial resources consumed by participating in them. This led some to question the audits' legitimacy, which could limit scope for improvements based on audit data, proposed by clinical teams. Conclusions Measures to enhance the audits' perceived legitimacy could help address these limitations. These include audit suppliers moving from an emphasis on cumulative, retrospective reports to real‐time reporting, clearly presenting the “headline” outcomes important to institutional bodies and staff. Measures may also include further negotiation between hospitals, suppliers and their commissioners about the nature and volume of data the latter are expected to collect; wider use by hospitals of routine clinical data to populate audit data fields; and further development of interactive digital technologies to help staff explore and report audit data in meaningful ways