Impact of Tandem Repeats on the Scaling of Nucleotide Sequences

Techniques such as detrended fluctuation analysis (DFA) and its extensions have been widely used to determine the nature of scaling in nucleotide sequences. In this brief communication we show that tandem repeats which are ubiquitous in nucleotide sequences can prevent reliable estimation of possible long-range correlations. Therefore, it is important to investigate the presence of tandem repeats prior to scaling exponent estimation.Comment: 14 Pages, 3 Figure

Mechanical-engineering aspects of mirror-fusion technology

The mirror approach to magnetic fusion has evolved from the original simple mirror cell to today's mainline effort: the tandem-mirror machine with thermal barriers. Physics and engineering research is being conducted throughout the world, with major efforts in Japan, the USSR, and the US. At least one facility under construction (MFTF-B) will approach equivalent energy breakeven in physics performance. Significant mechanical engineering development is needed, however, before a demonstration reactor can be constructed. The principal areas crucial to mirror reactor development include large high-field superconducting magnets, high-speed continuous vacuum-pumping systems, long-pulse high-power neutral-beam and rf-plasma heating systems, and efficient high-voltage high-power direct converters. Other areas common to all fusion systems include tritium handling technology, first-wall materials development, and fusion blanket design

Pulsed laser deposition growth of heteroepitaxial YBa2Cu3O7/La0.67Ca0.33MnO3 superlattices on NdGaO3 and Sr0.7La0.3Al0.65Ta0.35O3 substrates

Heteroepitaxial superlattices of [YBa2Cu3O7(n)/ La0.67Ca0.33MnO3(m)]x, where n and m are the number of YBCO and LCMO monolayers and x the number of bilayer repetitions, have been grown with pulsed laser deposition on NdGaO3 (110) and Sr0.7La0.3Al0.65Ta0.35O3 (LSAT) (001). These substrates are well lattice matched with YBCO and LCMO and, unlike the commonly used SrTiO3, they do not give rise to complex and uncontrolled strain effects due to structural transitions at low temperature. The growth dynamics and the structure have been studied in-situ with reflection high energy electron diffraction (RHEED) and ex-situ with scanning transmission electron microscopy (STEM), x-ray diffraction, and neutron reflectometry. The individual layers are found to be flat and continuous over long lateral distances with sharp and coherent interfaces and with a well-defined thickness of the individual layer. The only visible defects are antiphase boundaries in the YBCO layers that originate from perovskite unit cell height steps at the interfaces with the LCMO layers. We also find that the first YBCO monolayer at the interface with LCMO has an unusual growth dynamics and is lacking the CuO chain layer while the subsequent YBCO layers have the regular Y-123 structure. Accordingly, the CuO2 bilayers at both the LCMO/YBCO and the YBCO/LCMO interfaces are lacking one of their neighboring CuO chain layers and thus half of their hole doping reservoir. Nevertheless, from electric transport measurements on asuperlattice with n=2 we obtain evidence that the interfacial CuO2 bilayers remain conducting and even exhibit the onset of a superconducting transition at very low temperature. Finally, we show from dc magnetization and neutron reflectometry measurements that the LCMO layers are strongly ferromagnetic

Microarray analysis of human leucocyte subsets: the advantages of positive selection and rapid purification.

BACKGROUND: For expression profiling to have a practical impact in the management of immune-related disease it is essential that it can be applied to peripheral blood cells. Early studies have used total peripheral blood mononuclear cells, and as a consequence the majority of the disease-related signatures identified have simply reflected differences in the relative abundance of individual cell types between patients and controls. To identify cell-specific changes in transcription it would be necessary to profile purified leucocyte subsets. RESULTS: We have used sequential rounds of positive selection to isolate CD4 and CD8 T cells, CD19 B cells, CD14 monocytes and CD16 neutrophils for microarray analysis from a single blood sample. We compared gene expression in cells isolated in parallel using either positive or negative selection and demonstrate that there are no significant consistent changes due to positive selection, and that the far inferior results obtained by negative selection are largely due to reduced purity. Finally, we demonstrate that storing cells prior to separation leads to profound changes in expression, predominantly in cells of the myeloid lineage. CONCLUSION: Leukocyte subsets should be prepared for microarray analysis by rapid positive selection.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Constraints on gamma-ray burst and supernova progenitors through circumstellar absorption lines. (II): Post-LBV Wolf-Rayet stars

Van Marle et al. (2005) showed that circumstellar absorption lines in early Type Ib/c supernova and gamma-ray burst afterglow spectra may reveal the progenitor evolution of the exploding Wolf-Rayet star. While the quoted paper deals with Wolf-Rayet stars which evolved through a red supergiant stage, we investigate here the initially more massive Wolf-Rayet stars which are thought to evolve through a Luminous Blue Variable (LBV) stage. We perform hydrodynamic simulations of the evolution of the circumstellar medium around a 60 Msol star, from the main sequence through the LBV and Wolf-Rayet stages, up to core collapse. We then compute the column density of the circumstellar matter as a function of radial velocity, time and angle. This allows a comparison with the number and blue-shifts, of absorption components in the spectra of LBVs, Wolf-Rayet stars, Type Ib/c supernovae and gamma-ray burst afterglows. Our simulation for the post-LBV stage shows the formation of various absorption components, which are, however, rather short lived; they dissipate on time scales shorter than 50,000yr. As the LBV stage is thought to occur at the beginning of core helium burning, the remaining Wolf-Rayet life time is expected to be one order of magnitude larger. When interpreting the absorption components in the afterglow spectrum of GRB-021004 as circumstellar, it can be concluded that the progenitor of this source did most likely not evolve through an LBV stage. However, a close binary with late common-envelope phase (Case C) may produce a circumstellar medium that closely resembles the LBV to Wolf-Rayet evolution, but with a much shorter Wolf-Rayet period.Comment: accepted for publication by A&

Improvements to the Tendon-Actuated Lightweight In-Space MANipulator (TALISMAN)

Devices for manipulating and precisely placing payloads are critical for efficient space operations including berthing of spacecraft, in-space assembly, construction and repair. Key to the success of many NASA space activities has been the availability of long-reach crane-like devices such as the Shuttle Remote Manipulation System (SRMS) and the Space Station Remote Manipulation System (SSRMS). These devices have been used for many operations including berthing visiting spacecraft to the International Space Station, deployment of spacecraft, space station assembly, astronaut positioning, payload transfer, and spacecraft inspection prior to atmospheric re-entry. Retiring the Space Transportation System has led to the removal of the SRMS from consideration for in-space missions, thus creating a capability gap. Recognizing this gap, work was initiated at NASA on a new architecture for long-reach space manipulators. Most current devices are constructed by joining revolute joints with carbon composite tubes, with the joints accounting for the majority of the device mass. For example in the case of the SRMS, the entire device mass is 410 kg (904 lbm); the joint structure, motors, gear train, cabling, etc., accounts for the majority of the system mass because the carbon composite tubes mass is 46 kg (101 lbm). An alternate space manipulator concept, the Tendon-Actuated Lightweight In-Space MANipulator (TALISMAN) was created to address deficiencies in the current state-of-the-art in long-reach manipulators. The antagonistic tendon actuated joint architecture allows the motors actuating the joint to be removed from the joint axis, which simplifies the joint design while simultaneously providing mechanical advantage for the motors. The improved mechanical advantage, in turn, reduces the size and power requirements for the motor and gear train. This paper will describe recent architectural improvements to the TALISMAN design that: 1) improve the operational robustness of the system by enabling maneuvers not originally possible by varying the TALISMAN geometry; 2) enable efficient active antagonistic control of a joint while sharing cable between antagonistic tension networks; and 3) uses a unique arrangement of differential capstans to reduce motor torque requirements by an order of magnitude. The paper will also summarize recent efforts to enable autonomous deployment of a TALISMAN including the deployment concept of operations and associated hardware system design. The deployment forces are provided by the same motor systems that are used for articulation, thus reducing the mass associated with the deployment system. The deployment approach is being tested on a TALISMAN prototype which is designed to provide the same operational performance as a shuttle-class manipulator. The prototype has been fabricated and is operational in a new facility at NASA Langley Research Center that has a large area (15.2 m by 21.3 m [50 ft by 70 ft]) air-bearing floor

ELT Observations of Supernovae at the Edge of the Universe

We discuss the possibility of using Supernovae as tracers of the star formation history of the Universe for the range of stellar masses $\sim 3-30$ M$_\odot$ and possibly beyond. We simulate the observations of 350 SNe, up to $z\sim 15$, made with OWL (100m) telescope.Comment: 9 pages, 6 figures. To appear in "Exploring the Cosmic Frontier: Astrophysical Instruments for the 21st Century", proceedings of the conference held in Berlin, 18-21 May 200