Effects of salt stress on pigment and total soluble protein contents of three different tomato cultivars

WOS: 000281511100022In this study, the effects of salt stress on pigment and total soluble protein contents were investigated in different varieties of tomato (Lycopersicon esculentum Mill.). The seedlings of L. esculentum viz. Hazera, Dalli Tokat and Argy were treated with NaCl at 25, 50, 100, 125, 150 and 200 mM concentrations for 96 h with 24 h interval. Pigment and total soluble protein contents of all tomato cultivars were significantly decreased by salt stress depending on time intervals and salt concentrations. Decreasing of pigment and total soluble protein contents were more evident in Hazera under short time salt exposure. Pigment content of Argy plants were less affected by salt concentration and exposure time. The results of this study suggest that the Argy cultivars are relatively better protected under salt stress conditions than Dalli tokat and Hazera cultivars

Nonoccupational Exposure of Agricultural Area Residents to Pesticides: Pesticide Accumulation and Evaluation of Genotoxicity

Doganlar, Zeynep Banu (Trakya author) Doganlar, Oguzhan (Trakya author) Tozkir, Hilmi (Trakya author) Gokalp, Fulya Dilek (Trakya author) Dogan, Ayten (Trakya author) Yamac, Ferah (Trakya author)Although many studies related the toxic effects of pesticides on agricultural workers, little research has been done about agricultural area residents. The purpose of this work was to monitor the presence of pesticides, as well as their genotoxic and cytotoxic potential, in humans with blood samples collected from control and intensive agricultural areas in the Thrace region. Pesticide accumulations were determined by LC-MS/MS. Cytotoxicity and genotoxicity were analyzed by comet assay, and the effect of pesticide accumulation on oxidative stress, DNA repair, and molecular chaperone response were analyzed by qRT-PCR assays in the human blood samples. The agricultural area residents had a significantly higher concentration of pesticides than those in the control area at all three sampling times, and the total pesticide amounts were 4.3 and 10 times significantly higher in blood sampled in the pesticide use period (August 2015 and 2016, respectively) than in the nonuse period (November 2015). The results showed that the pesticide level in blood during the use period led to oxidative stress, DNA damage (mean comet length and % tail DNA), and unfolded/misfolded protein response. Particularly, in pesticide use season, difference between these parameters was found statistically significant with comparison to control. Our results indicate that individuals residing around a monoculture rice farming area comprise an at-risk group as a result of increased genotoxicity evidenced in human blood. We suggest that biological monitoring efforts should be used to control nonoccupational exposures to pesticides and thus safeguard the health of agricultural area residents