Effect of weightlessness conditions on the somatic embryogenesis in the culture of carrot cells

A carrot cell culture seeded in Petri dishes in the United States and transported to the USSR was subjected to weightlessness for 20 days during the flight of Kosmos 782. The controls were cultures placed on a centrifuge (1 g) inside the satellite and cultures left on ground in the U.S.S.R. and the United States. A count of structures in the dishes after the flight showed that the number of developing embryonic structures and the extent of their differentiation in weightlessness did not reliably differ from the number and extent of differentiation in structures developed on the ground. Structures with long roots developed in weightlessness. Analysis of the root zones showed that these roots differed by the increased size of the zone of differentiated cells. The increased size of the zones of differentiated cells can indicate earlier development of embryonic structures

A Minimally Invasive Method for the Treatment of Post-Traumatic Disorders of the Bone Union of the Tibia

Background. The ability to control regeneration processes is a key link in the development of methods for treating patients with post-traumatic disorders of reparative osteogenesis. Aim of the study. To evaluate the effectiveness of the fusion of the tibia by local application of the interferon-beta preparation with autogenous bone marrow punctate in post-traumatic disorders of reparative regeneration.Material and methods. The analysis of the effectiveness of clinical application of autogenous bone marrow with interferon-beta-1b in 64 patients with delayed consolidation and pseudarthrosis of the tibia was carried out. All patients underwent external bone fixation. In order to optimize reparative osteogenesis during the operation, a mixture containing 3–4 ml of bone marrow with 8 million IU/0.5 ml of interferon beta-1b solution was injected locally into the interfragmental space.Results. With puncture local injection of interferon-beta with punctate of autogenous bone marrow with  delayed consolidation, fusion of the tibial fracture was achieved in 100 % of cases. In patients with a diagnosis  of a pseudarthrosis of the tibia, a positive result was achieved in 90.3 % of cases. Thus, the local application of autogenous bone marrow together with the interferon-beta preparation made it possible to achieve bone fusion in post-traumatic disorders of bone regeneration in 95.2 % of cases.Conclusion. With delayed consolidation and developing hypertrophic pseudoarthrosis in conditions of stable fixation with anatomical reposition of fragments, puncture autotransplantation of a mixture of bone marrow with a solution of interferon-beta is proposed

Диагностическая значимость белка S100B при критических состояниях

The review deals with possibilities for the quantitative assessment of the degree of brain injury, by measuring the level of S100B protein in various biological fluids. Data are given on changes in the concentration of this protein in different categories of patients, including those who are critically ill. Key words: S100B protein, laboratory diagnosis of critical conditions, biomarkers, perinatal brain lesions.Обзор посвящен возможности количественной оценки степени повреждений мозга путем измерения уровня белка S100B в различных биологических жидкостях. Приводятся данные по изменению концентрации данного протеина у разных категорий больных, в том числе — находящихся в критическом состоянии. Ключевые слова: S100B, лабораторная диагностика критических состояний, биомаркеры, перинатальные повреждения головного мозга

Функциональная значимость полиморфизма генов ApoE и SOD2 в формировании хронической HCV-инфекции

The analysis of gene polymorphism plays an important role in assessment of disposition to infectious diseases at the population and individual level. In this paper, the frequencies of allelic versions of apolipoprotein E and 2nd type superoxide dismutase genes and the corresponding genotypes were determined in healthy persons and chronic viral hepatitis type C patients of the Europeoid population of the Tomsk Region. For the analysis of polymorphism of these genes, we used modern genetic methods: polymerase chain reaction and polymorphism of lengths of restriction fragments. For the studied population, it was revealed that the polymorphism of apolipoprotein E and 2nd type superoxide dismutase genes correlates with the development of chronic viral hepatitis type C and with the fibrogenesis process.Анализ полиморфизма генов играет важную роль в оценке предрасположенности к инфекционным заболеваниям на популяционном и индивидуальном уровне. В настоящей работе проведено определение частот аллельных вариантов генов аполипопротеина Е и супероксиддисмутазы 2-го типа и соответствующих генотипов у здоровых и больных хроническим вирусным гепатитом С представителей европеоидной популяции Томской области. Для анализа полиморфизма данных генов использовали современные генетические методы исследования: полимеразную цепную реакцию и полиморфизм длин рестрикционных фрагментов. Для изученной популяций выявлена связь полиморфизма гена аполипопротеина Е и супероксиддисмутазы 2-го типа с развитием хронического вирусного гепатита С и процессом фиброгенеза

Radiation semiotics of disseminated pulmonary tuberculosis

The aim of the study was to optimize the radiation diagnosis and differentiation of disseminated pulmonary tuberculosis (DTL) by studying the ray lung semiotics using multislice computed tomography (MSCT), radionuclide diagnostics (RND) with the use of a radiopharmaceutical (RFP) 99mTc-technetril and identifying the most informative for disseminated tuberculosis MSCT- RIA criteria. Materials and methods. The analysis of MSCT data was performed in 67 patients who had X-ray signs of pulmonary dissemination. The study was conducted on a multislice computer tomograph Somatom Emotion 16 from Siemens. 23 patients underwent a radionuclide study with a 99mTc-technetril radiopharmaceutical on the Nucline Spirit gamma camera in the planar and single-photon emission computerized tomography (SPECT) mode. The statistical processing of the data was carried out using the MicrosoftExcel software package. The reliability of the differences between the qualitative indices of the compared groups was determined using the χ2 criterion. Results. The distribution of foci of dissemination according to MSCT using the high resolution algorithm (HRCT) was characterized by localization of dissemination foci in the structures of the primary, secondary pulmonary lobe, perivascular and peribronchial, involvement of pleura piles of various degrees and reactive changes in the intrathoracic lymph nodes (VGLU). In 55.7% of cases, foci with DTL were distributed in intralobular structures, lobular septa and endobronchial. In 86.3%, fragmentary infiltration of axial interstitium was noted. There was also marked infiltration of pleura sheets involving extrapuleural fat tissue in 50.7% of cases. Hyperplasia of the intrathoracic lymph nodes was established in 12.5% of cases. According to planar and SPECT studies in 21.0%, the prevalence of dissemination by CT correlated with accumulation of RFP in the lungs according to planar research and topically - in VGLU according to SPECT. In 81.8% of cases of hyperplasia of lymph nodes revealed by CT, there was an accumulation of RFP by SPECT. In 42.1% of cases of limited dissemination, according to CT, there was a diffuse accumulation of RFP following the results of planar scintigraphy. Conclusion Interpretation of the obtained data, taking into account the level of damage to the structural units of the lung with the definition of activity and prevalence in the application of different radiotherapy techniques, allows to optimize the diagnosis and differential diagnosis of disseminated pulmonary tuberculosis.Цель исследования - оптимизация лучевой диагностики и дифференциации диссеминированного туберкулеза легких (ДТЛ) посредством изучения лучевой семиотики лёгких методом мультисрезовой компьютерной томографии (МСКТ), радионуклидной диагностики (РНД) с применением радиофармпрепарата (РФП) 99mTс-технетрила и выявление наиболее информативных для диссеминированного туберкулёза МСКТ-РНД критериев. Материалы и методы. Проведен анализ данных МСКТ исследования 67 больных, у которых определялись рентгенологические признаки легочной диссеминации. Исследование проведено на мультисрезовом компьютерном томографе Somatom Emotion 16 фирмы Siemens. 23 пациентам проведено радионуклидное исследование с радиофармпрепаратом 99mTс-технетрилом на гамма-камере Nucline Spirit в планарном и однофотонном эмиссионном компьютерном томографическом (ОФЭКТ) режиме. Статистическую обработку данных осуществляли с помощью пакета программ «MicrosoftExcel». Определение достоверности различий между качественными показателями сравниваемых групп проводили с помощью критерия в2. результаты. распределение очагов диссеминации по данным МСКТ с использованием алгоритма высокого разрешения (КТВР), характеризовалось локализацией очагов диссеминации в структурах первичной, вторичной легочной дольки, периваскулярно и перибронхиально, вовлечении листков плевры различной степени и реактивными изменениями внутригрудных лимфатических узлов (ВГЛУ). В 55,7% случаев очаги при ДТЛ распространялись во внутридольковых структурах, дольковых перегородках и эндобронхиально. В 86,3% отмечалась фрагментарная инфильтрация аксиального интерстиция. отмечалась также выраженная инфильтрация листков плевры с вовлечением экстраплевральной жировой клетчатки в 50,7% случаев. Гиперплазия внутригрудных лимфатических узлов установлена в 12,5% случаев. По данным планарного и ОФЭКТ исследования в 21,0% распространенность диссеминации по КТ коррелировала с накоплением РФП в легких по данным планарного исследования и топически - во ВГЛУ по данным ОФЭКТ. В 81,8% выявленных по КТ случаях гиперплазии лимфатических узлов отмечено накопление РФП по ОФЭКТ. В 42,1% случаев ограниченной диссеминации по данным КТ отмечалось диффузное накопление РФП по результатам планарной сцинтиграфии. Заключение Интерпретация полученных данных с учетом уровня поражения структурных единиц легкого с определением активности и распространенности при применении разных лучевых методик позволяет оптимизировать диагностику и дифференциальную диагностику диссеминированного туберкулеза легких

A Massive Open Online Course on Particle Accelerators

International audienceThe TIARA (Test Infrastructure and Accelerator Research Area) project funded by the European Union 7th framework programme made a survey of provision of education and training in accelerator science in Europe. This survey highlighted the need for more training opportunities targeting undergraduate-level students. This need is now being addressed by the European Union H2020 project ARIES (Accelerator Research and Innovation for European Science and Society) via the preparation of a Massive Online Open Course (MOOC) on particle accelerator science and engineering. We present here the current status of this project, the main elements of the syllabus, how it will be delivered, and the schedule for providing the course

Diagnostic Value of S100B Protein in Critical Conditions

The review deals with possibilities for the quantitative assessment of the degree of brain injury, by measuring the level of S100B protein in various biological fluids. Data are given on changes in the concentration of this protein in different categories of patients, including those who are critically ill. Key words: S100B protein, laboratory diagnosis of critical conditions, biomarkers, perinatal brain lesions

Functional significance of polymorphism of ApoE and SOD2 genes in formation of chronic HCV infection

The analysis of gene polymorphism plays an important role in assessment of disposition to infectious diseases at the population and individual level. In this paper, the frequencies of allelic versions of apolipoprotein E and 2nd type superoxide dismutase genes and the corresponding genotypes were determined in healthy persons and chronic viral hepatitis type C patients of the Europeoid population of the Tomsk Region. For the analysis of polymorphism of these genes, we used modern genetic methods: polymerase chain reaction and polymorphism of lengths of restriction fragments. For the studied population, it was revealed that the polymorphism of apolipoprotein E and 2nd type superoxide dismutase genes correlates with the development of chronic viral hepatitis type C and with the fibrogenesis process

Remote monitoring of sea surface HFSWR

The paper considers an excellent electrodynamics model of an excited sea surface when it is interpreted by spectral components of the backscattered HF signal for remotely monitored targets. A peculiarity of the application of the electrodynamics model is supposing that the excited sea surface section is a quasi-periodic random antenna array with elementary resonators