535 research outputs found
IDeF-X ASIC for Cd(Zn)Te spectro-imaging systems
Joint progresses in Cd(Zn)Te detectors, microelectronics and interconnection
technologies open the way for a new generation of instruments for physics and
astrophysics applications in the energy range from 1 to 1000 keV. Even working
between -20 and 20 degrees Celsius, these instruments will offer high spatial
resolution (pixel size ranging from 300 x 300 square micrometers to few square
millimeters), high spectral response and high detection efficiency. To reach
these goals, reliable, highly integrated, low noise and low power consumption
electronics is mandatory. Our group is currently developing a new ASIC detector
front-end named IDeF-X, for modular spectro-imaging system based on the use of
Cd(Zn)Te detectors. We present here the first version of IDeF-X which consists
in a set of ten low noise charge sensitive preamplifiers (CSA). It has been
processed with the standard AMS 0.35 micrometer CMOS technology. The CSA are
designed to be DC coupled to detectors having a low dark current at room
temperature. The various preamps implemented are optimized for detector
capacitances ranging from 0.5 up to 30 pF.Comment: 8 pages, 11 figures, IEEE NSS-MIC conference in Rome 2004, submitted
to IEEE TNS, correction in unit of figure
Magnetoresistance in Single Layer Graphene: Weak Localization and Universal Conductance Fluctuation Studies
We report measurements of magnetoresistance in single-layer graphene as a
function of gate voltage (carrier density) at 250 mK. By examining signatures
of weak localization (WL) and universal conductance fluctuations (UCF), we find
a consistent picture of phase coherence loss due to electron-electron
interactions. The gate-dependence of the elastic scattering terms suggests that
the effect of trigonal warping, i.e., the non-linearity of the dispersion
curves, may be strong at high carrier densities, while intra-valley scattering
may dominate close to the Dirac point. In addition, a decrease in UCF amplitude
with decreasing carrier density can be explained by a corresponding loss of
phase coherence
Visualizing landscapes of the superconducting gap in heterogeneous superconductor thin films: geometric influences on proximity effects
The proximity effect is a central feature of superconducting junctions as it
underlies many important applications in devices and can be exploited in the
design of new systems with novel quantum functionality. Recently, exotic
proximity effects have been observed in various systems, such as
superconductor-metallic nanowires and graphene-superconductor structures.
However, it is still not clear how superconducting order propagates spatially
in a heterogeneous superconductor system. Here we report intriguing influences
of junction geometry on the proximity effect for a 2D heterogeneous
superconductor system comprised of 2D superconducting islands on top of a
surface metal. Depending on the local geometry, the superconducting gap induced
in the surface metal region can either be confined to the boundary of the
superconductor, in which the gap decays within a short distance (~ 15 nm), or
can be observed nearly uniformly over a distance of many coherence lengths due
to non-local proximity effects.Comment: 17 pages, 4 figure
Vortex dynamics in layered superconductors with correlated defects: influence of interlayer coupling
We report a detailed study of the vortex dynamics and vortex phase diagrams
of two amorphous Ta_0.3Ge_0.7/Ge multilayered films with intrinsic coplanar
defects, but different interlayer coupling. A pinned Bose-glass phase in the
more weakly coupled sample exists only below a cross-over field H* in striking
contrast to the strongly coupled film. Above H* the flux lines are thought to
break up into pancake vortices and the cross-over field is significantly
increased when the field is aligned along the extended defects. The two films
show different vortex creep excitations in the Bose-glass phase.Comment: zip file: 1 RevTex, 5 figures (png
Caries associated with orthodontic care part 2: management
It is recognized that wearing an orthodontic appliance increases the caries risk of the individual. The prevalence of demineralization has been reported to be as high as 73%. When demineralization occurs a number of treatments exist: fluoride application, acid microabrasion, casein phosphopeptide-amorphous calcium phosphate (CCP-ACP), resin infiltration and self-assembling peptides. Of these, topical fluoride has the most evidence to support its use.
CPD/Clinical Relevance: Demineralization is the most common complication of orthodontic care. The clinician should understand how to manage this when it occurs
Bacterial Symbiosis Maintenance in the Asexually Reproducing and Regenerating Flatworm Paracatenula galateia
Bacteriocytes set the stage for some of the most intimate interactions between animal and bacterial cells. In all bacteriocyte possessing systems studied so far, de novo formation of bacteriocytes occurs only once in the host development, at the time of symbiosis establishment. Here, we present the free-living symbiotic flatworm Paracatenula galateia and its intracellular, sulfur-oxidizing bacteria as a system with previously undescribed strategies of bacteriocyte formation and bacterial symbiont transmission. Using thymidine analogue S-phase labeling and immunohistochemistry, we show that all somatic cells in adult worms – including bacteriocytes – originate exclusively from aposymbiotic stem cells (neoblasts). The continued bacteriocyte formation from aposymbiotic stem cells in adult animals represents a previously undescribed strategy of symbiosis maintenance and makes P. galateia a unique system to study bacteriocyte differentiation and development. We also provide morphological and immunohistochemical evidence that P. galateia reproduces by asexual fragmentation and regeneration (paratomy) and, thereby, vertically transmits numerous symbiont-containing bacteriocytes to its asexual progeny. Our data support the earlier reported hypothesis that the symbiont population is subjected to reduced bottleneck effects. This would justify both the codiversification between Paracatenula hosts and their Candidatus Riegeria symbionts, and the slow evolutionary rates observed for several symbiont genes
Proximity-dependent initiation of hybridization chain reaction
Sensitive detection of protein interactions and post-translational modifications of native proteins is a challenge for research and diagnostic purposes. A method for this, which could be used in point-of-care devices and high-throughput screening, should be reliable, cost effective and robust. To achieve this, here we design a method (proxHCR) that combines the need for proximal binding with hybridization chain reaction (HCR) for signal amplification. When two oligonucleotide hairpins conjugated to antibodies bind in close proximity, they can be activated to reveal an initiator sequence. This starts a chain reaction of hybridization events between a pair of fluorophore-labelled oligonucleotide hairpins, generating a fluorescent product. In conclusion, we show the applicability of the proxHCR method for the detection of protein interactions and posttranslational modifications in microscopy and flow cytometry. As no enzymes are needed, proxHCR may be an inexpensive and robust alternative to proximity ligation assays
Establishment, molecular and biological characterization of HCB-514: a novel human cervical cancer cell line
Cervical cancer is the fourth most common cancer in women. Although cure rates are high for early stage disease, clinical outcomes for advanced, metastatic, or recurrent disease remain poor. To change this panorama, a deeper understanding of cervical cancer biology and novel study models are needed. Immortalized human cancer cell lines such as HeLa constitute crucial scientific tools, but there are few other cervical cancer cell lines available, limiting our understanding of a disease known for its molecular heterogeneity. This study aimed to establish novel cervical cancer cell lines derived from Brazilian patients. We successfully established one (HCB-514) out of 35 cervical tumors biopsied. We confirmed the phenotype of HCB-514 by verifying its' epithelial and tumor origin through cytokeratins, EpCAM and p16 staining. It was also HPV-16 positive. Whole-exome sequencing (WES) showed relevant somatic mutations in several genes including BRCA2, TGFBR1 and IRX2. A copy number variation (CNV) analysis by nanostring and WES revealed amplification of genes mainly related to kinases proteins involved in proliferation, migration and cell differentiation, such as EGFR, PIK3CA, and MAPK7. Overexpression of EGFR was confirmed by phospho RTK-array and validated by western blot analysis. Furthermore, the HCB-514 cell line was sensitive to cisplatin. In summary, this novel Brazilian cervical cancer cell line exhibits relevant key molecular features and constitutes a new biological model for pre-clinical studies.Barretos Cancer Hospital Research Support Department (NAP) for sample collection, Barretos Cancer Hospital Biobank for sample processing, Dr. Flávia de Paula and Gabriela Fernandes for technical support of STRs and BRCA2 Sanger validation, respectively, and Dr. Laura Musselwhite (Duke University) for revising the manuscript. This study was supported by grants from the FINEP (MCTI/FINEP/MS/SCTIE/DECIT-01/2013 - FPXII- BIOPLAT - Process number 01.13.0469.00) and Barretos Cancer Hospital. PhD scholarship from FINEP (Grant numbers 384088/2014-7 and 380434/2015-6) and Barretos Cancer Hospital to MNR
A whey protein-based multi-ingredient nutritional supplement stimulates gains in lean body mass and strength in healthy older men: A randomized controlled trial
Protein and other compounds can exert anabolic effects on skeletal muscle, particularly in conjunction with exercise. The objective of this study was to evaluate the efficacy of twice daily consumption of a protein-based, multi-ingredient nutritional supplement to increase strength and lean mass independent of, and in combination with, exercise in healthy older men. Forty-nine healthy older men (age: 73 ± 1 years [mean ± SEM]; BMI: 28.5 ± 1.5 kg/m2) were randomly allocated to 20 weeks of twice daily consumption of either a nutritional supplement (SUPP; n = 25; 30 g whey protein, 2.5 g creatine, 500 IU vitamin D, 400 mg calcium, and 1500 mg n-3 PUFA with 700 mg as eicosapentanoic acid and 445 mg as docosahexanoic acid); or a control (n = 24; CON; 22 g of maltodextrin). The study had two phases. Phase 1 was 6 weeks of SUPP or CON alone. Phase 2 was a 12 week continuation of the SUPP/CON but in combination with exercise: SUPP + EX or CON + EX. Isotonic strength (one repetition maximum [1RM]) and lean body mass (LBM) were the primary outcomes. In Phase 1 only the SUPP group gained strength (Σ1RM, SUPP: +14 ± 4 kg, CON: +3 ± 2 kg, P < 0.001) and lean mass (LBM, +1.2 ± 0.3 kg, CON: -0.1 ± 0.2 kg, P < 0.001). Although both groups gained strength during Phase 2, upon completion of the study upper body strength was greater in the SUPP group compared to the CON group (Σ upper body 1RM: 119 ± 4 vs. 109 ± 5 kg, P = 0.039). We conclude that twice daily consumption of a multi-ingredient nutritional supplement increased muscle strength and lean mass in older men. Increases in strength were enhanced further with exercise training
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