An investigation into the fertilizer potential of slaughterhouse cattle paunch

In Australia, the red meat processing industry actively seeks approaches to improve the management of solid waste from processing operations and enhance the environmental performance. Recycling of paunch waste to farmland could be a cost-effective and practicable environmental option. However, little is known about the agronomic value of fresh and composted paunch, and the associated requirements for land application. Therefore, a short-term experimental work was undertaken to assess potential risks due to weed seed contamination and determine the agronomic response of ryegrass (Lolium perenne L.) to soil incorporation of paunch. The risk of weed contamination from soil application of paunch appeared to be low; however, methods that account for viability of seeds may be required to fully discard such a risk. Soil application of paunch at field equivalent rates of 150-300 kg ha-1 of N increased dry matter yield by ≈30% on average compared with untreated grass, but was approximately 35% lower than a mineral fertilizer treatment applied at the same rates. Dry matter yield of paunch-treated grass was between 2000 and 3000 kg per ha over four consecutive cuts at 25-day intervals. Nitrogen use-efficiency of paunch was approximately 10% (range: 3% to 20%, depending on paunch type), and total N in harvested plant material showed values, which were between 2% and 3%. Overall, there appears to be potential for paunch-derived products to be used as a source of carbon and nutrients in crop production. Areas that merit a research priority within this space are also outlined in this paper. Such work is required to inform soil-, climate- and crop-specific land application rates, optimize agronomic performance, and minimize environmental concerns. There is also a requirement for the value proposition to industry to be determined, including reduced cost of disposal of material via gate fees and fertilizer replacement value

Field-scale evaluation of biosolids-derived organomineral fertilizers applied to winter wheat in England

Field-scale experiments in four crop seasons established the agronomic performance of biosolids-derived organomineral fertilizers (OMF) for winter wheat (Triticum aestivum L.) production in England. Two OMF formulations (OMF10 10:4:4 and OMF15 15:4:4) were compared with urea and biosolids granules (≈5:6:0.2) to determine crop responses and fertilizer effects on soil chemical properties. Fertilizers were applied at N rates between 0 and 250 kg ha–1 at regular increments of 50 kg ha–1 N. Average grain yields with OMF10 and OMF15 were higher than with biosolids granules, but lower than with urea (P < 0.05). The optimum N application rates, and corresponding grain yields, were 245 and 7900 kg ha–1 for biosolids, 257 and 9100 kg ha–1 for OMF10, 249 and 9500 kg ha–1 for OMF15, and 225 and 10350 kg ha–1 for urea, respectively. Differences in grain yield between fertilizer treatments were explained by differences in yield components, particularly number of grains and thousand-grain-weight. Grain-N recoveries were 31% for biosolids, ≈40% for OMF, and 52% for urea. Organomineral fertilizers-induced changes in soil extractable P and soil P Index were not significant. Thus, application of OMF replenished P offtake by the crop and therefore supported the choice of the proposed OMF formulations. By contrast, extractable P increased in biosolids and decreased in urea-treated soils, respectively. Heavy metals in soil were unaffected by fertilizer treatment and lower than permissible limit values. The use of OMF for winter wheat production appears to be a sustainable approach to recycling biosolids to land

Deciphering the genome structure and paleohistory of _Theobroma cacao_

We sequenced and assembled the genome of _Theobroma cacao_, an economically important tropical fruit tree crop that is the source of chocolate. The assembly corresponds to 76% of the estimated genome size and contains almost all previously described genes, with 82% of them anchored on the 10 _T. cacao_ chromosomes. Analysis of this sequence information highlighted specific expansion of some gene families during evolution, for example flavonoid-related genes. It also provides a major source of candidate genes for _T. cacao_ disease resistance and quality improvement. Based on the inferred paleohistory of the T. cacao genome, we propose an evolutionary scenario whereby the ten _T. cacao_ chromosomes were shaped from an ancestor through eleven chromosome fusions. The _T. cacao_ genome can be considered as a simple living relic of higher plant evolution

Radical penectomy, a compromise for life: Results from the PECAD study

Background: The use of organ sparing strategies to treat penile cancer (PC) is currently supported by evidence that has indicated the safety, efficacy and benefit of this surgery. However, radical penectomy still represents up to 15-20% of primary tumor treatments in PC patients. The aim of the study was to evaluate efficacy in terms of overall survival (OS) and disease-free survival (DFS) of radical penectomy in PC patients.Methods: Data from a retrospective multicenter study (PEnile Cancer ADherence study, PECAD Study) on PC patients treated at 13 European and American urological centers (Hospital "Sant'Andrea", Sapienza University, Roma, Italy; "G.D'Annunzio" University, Chieti and ASL 2 Abruzzo, Hospital "S. Pio da Pietrelcina", Vasto, Italy; Department of Genitourinary Oncology, Moffitt Cancer Center, Tampa, FL, USA; Hospital of Budapest, Hungary; Department of Emergency and Organ Transplantation, Urology and Andrology Unit II, University of Bari, Italy; Hospital "Spedali Civil", Brescia, Italy; Istituto Europeo di Oncologia, University of Milan, Milan, Italy; University of Modena &amp; Reggio Emilia, Modena, Italy; Hospital Universitario La Paz, Madrid, Spain; Ceara Cancer Institute, Fortaleza, Brazil; Virginia Commonwealth University, Richmond, VA, USA; Aristotle University of Thessaloniki, Thessaloniki, Greece; Maria Sklodowska-Curie Memorial Cancer Center, Warsaw, Poland) between 2010 and 2016 were used. Medical records of patients who specifically underwent radical penectomy were reviewed to identify main clinical and pathological variables. Kaplan-Meier method was used to estimate 1- and 5-year OS and DFS.Results: Of the entire cohort of 425 patients, 72 patients (16.9%) treated with radical penectomy were extracted and were considered for the analysis. The median age was 64.5 (IQR, 57.5-73.2) years. Of all, 41 (56.9%) patients had pT3/pT4 and 31 (43.1%) pT1/pT2. Moreover, 36 (50.0%) were classified as pN1-3 and 5 (6.9%) MI. Furthermore, 61 (84.7%) had a high grade (G2-G3) with 6 (8.3%) positive surgical margins. The 1- and 5-year OS rates were respectively 73.3% and 59.9%, while the 1- and 5-year DFS rates were respectively 67.3% and 35.1%.Conclusions: PC is an aggressive cancer particularly in more advanced stage. Overall, more than a third of patients do not survive at 5 years and more than 60% report a disease recurrence, despite the use of a radical treatment

Rarity of monodominance in hyperdiverse Amazonian forests.

Tropical forests are known for their high diversity. Yet, forest patches do occur in the tropics where a single tree species is dominant. Such "monodominant" forests are known from all of the main tropical regions. For Amazonia, we sampled the occurrence of monodominance in a massive, basin-wide database of forest-inventory plots from the Amazon Tree Diversity Network (ATDN). Utilizing a simple defining metric of at least half of the trees ≥ 10 cm diameter belonging to one species, we found only a few occurrences of monodominance in Amazonia, and the phenomenon was not significantly linked to previously hypothesized life history traits such wood density, seed mass, ectomycorrhizal associations, or Rhizobium nodulation. In our analysis, coppicing (the formation of sprouts at the base of the tree or on roots) was the only trait significantly linked to monodominance. While at specific locales coppicing or ectomycorrhizal associations may confer a considerable advantage to a tree species and lead to its monodominance, very few species have these traits. Mining of the ATDN dataset suggests that monodominance is quite rare in Amazonia, and may be linked primarily to edaphic factors

Radical penectomy, a compromise for life. Results from the PECAD study

Background: The use of organ sparing strategies to treat penile cancer (PC) is currently supported by evidence that has indicated the safety, efficacy and benefit of this surgery. However, radical penectomy still represents up to 15-20% of primary tumor treatments in PC patients. The aim of the study was to evaluate efficacy in terms of overall survival (OS) and disease-free survival (DFS) of radical penectomy in PC patients. Methods: Data from a retrospective multicenter study (PEnile Cancer ADherence study, PECAD Study) on PC patients treated at 13 European and American urological centers (Hospital “Sant'Andrea”, Sapienza University, Roma, Italy; “G.D'Annunzio” University, Chieti and ASL 2 Abruzzo, Hospital “S. Pio da Pietrelcina”, Vasto, Italy; Department of Genitourinary Oncology, Moffitt Cancer Center, Tampa, FL, USA; Hospital of Budapest, Hungary; Department of Emergency and Organ Transplantation, Urology and Andrology Unit II, University of Bari, Italy; Hospital “Spedali Civili”, Brescia, Italy; Istituto Europeo di Oncologia, University of Milan, Milan, Italy; University of Modena &amp; Reggio Emilia, Modena, Italy; Hospital Universitario La Paz, Madrid, Spain; Ceara Cancer Institute, Fortaleza, Brazil; Virginia Commonwealth University, Richmond, VA, USA; Aristotle University of Thessaloniki, Thessaloniki, Greece; Maria Skłodowska-Curie Memorial Cancer Center, Warsaw, Poland) between 2010 and 2016 were used. Medical records of patients who specifically underwent radical penectomy were reviewed to identify main clinical and pathological variables. Kaplan-Meier method was used to estimate 1- and 5-year OS and DFS. Results: Of the entire cohort of 425 patients, 72 patients (16.9%) treated with radical penectomy were extracted and were considered for the analysis. The median age was 64.5 (IQR, 57.5-73.2) years. Of all, 41 (56.9%) patients had pT3/pT4 and 31 (43.1%) pT1/pT2. Moreover, 36 (50.0%) were classified as pN1-3 and 5 (6.9%) M1. Furthermore, 61 (84.7%) had a high grade (G2-G3) with 6 (8.3%) positive surgical margins. The 1- and 5-year OS rates were respectively 73.3% and 59.9%, while the 1- and 5-year DFS rates were respectively 67.3% and 35.1%. Conclusions: PC is an aggressive cancer particularly in more advanced stage. Overall, more than a third of patients do not survive at 5 years and more than 60% report a disease recurrence, despite the use of a radical treatment

AUSÊNCIA DE CRESCIMENTO MICROBIANO EM AMOSTRAS DE LEITE OBTIDAS DE VACAS IDENTIFICADAS COM MASTITE: CASUÍSTICA DO LABORATÓRIO DE MICROBIOLOGIA VETERINÁRIA IFC CONCÓRDIA (FEVEREIRO A JULHO DE 2023)

O cultivo microbiológico padrão é uma das principais técnicas utilizadas para diagnosticar a etiologia de casos de mastite clínica e subclínicas em bovinos, a partir de amostras de leite. O crescimento de bactérias é influenciado por fatores genéticos, nutricionais, ambientais, pela concentração de íons de hidrogênio, disponibilidade de água, composição atmosférica e pela pressão osmótica. As condições de isolamento microbiológico in vitro favorecem o crescimento de grande parte dos agentes causadores de mastites, no entanto, é possível ocorrer ausência de crescimento microbiano em alguns casos. O objetivo do presente estudo foi avaliar a frequência de resultados de ausência de crescimento no cultivo microbiológico padrão, a partir de amostras de leite bovino encaminhadas para análise no Laboratório de Microbiologia do Instituto Federal Catarinense campus Concórdia, durante o período de fevereiro a julho de 2023. As amostras foram provenientes de rebanhos leiteiros da região meio oeste catarinense, e coletadas por médicos veterinários, a partir de vacas identificadas com mastite clínica ou subclínica. No total, foram avaliadas 381 amostras de leite, cultivadas em ágar sangue bovino a 5% e ágar MacConkey, com incubação a 37 graus Celsius em aerobiose e leituras a cada 24 horas durante 72 horas, com caracterização bioquímica dos isolados de acordo com a metodologia padrão. Do total de amostras analisadas, 119 (31,23%), ou seja, praticamente um terço destas, apresentaram ausência de crescimento microbiano. Dentre os principais fatores que podem estar envolvidos nestes resultados, ressalta-se: 1) Possibilidade da presença de resíduos de antimicrobianos nas amostras, o que pode inibir o crescimento dos microrganismos no cultivo padrão; 2) Presença de agentes fastidiosos ou microrganismos que não crescem em meios de cultura convencionais; 3) Quadros de mastite autolimitantes ou convalescentes, que estejam em fase de cura, onde a carga microbiana é realmente ausente ou encontra-se muito reduzida; 4) Quadros de mastite não infecciosos; 5) Identificação falso positiva de quadro de mastite nos animais; 6) Falhas na coleta das amostras; 7) Acondicionamento e transporte das amostras em condições inadequadas. Com base nos resultados obtidos no presente estudo, reforça-se a necessidade, por parte dos profissionais médicos veterinários, para a adequada identificação de casos de mastite em rebanhos leiteiros; a correta coleta e acondicionamento de amostras de leite para encaminhamento ao laboratório; o respeito ao período de carência (para vacas em tratamento com antimicrobianos) para realização da coleta de amostras de leite e, no caso de suspeita de infecções por microrganismos fastidiosos, o encaminhamento de amostras para análises mais acuradas, como a Reação em Cadeia pela Polimerase (PCR)