Patients with IBD have a more cautious attitude towards COVID-19 vaccination

BackgroundTo understand the awareness of COVID-19 vaccine, the willingness to vaccinate and the influencing factors of willingness to vaccinate in inflammatory bowel disease (IBD) patients.MethodsThe online questionnaire was distributed to conduct a survey to analyze and evaluate the willingness, awareness and trust in vaccines of IBD patients. Bivariate analyses and logistic regression models were used to analysis influencing factors.ResultsWe sent the questionnaire to the WeChat group for patient management and 304 patients responded, out of which 16 respondents had to be excluded and 288 respondents were included for the analysis. Among them, 209 patients vaccinated with COVID-19 vaccine. Among the non-vaccinated 79 patients, the main reasons for their concerns were afraid of vaccination aggravating IBD and fear of adverse effects. Our results showed that IBD patients with long disease duration were more willing to receive COVID-19 vaccination (P<0.05). We also observed that a high perception of benefits and cues to action to receive the vaccine were the two most important constructs affecting a definite intention for COVID-19 vaccination (P<0.05).ConclusionsPatients with IBD have a more cautious attitude towards COVID-19 vaccination, which may lead to a higher rate of vaccine hesitancy. Further efforts should be made to protect patients with IBD from COVID-19 infections and achieve adequate vaccination coverage

The Different Effects of Skeletal Muscle and Fat Mass on Height Increment in Children and Adolescents Aged 6–11 Years: A Cohort Study From China

ObjectiveThis study aimed to investigate the contribution of body composition including skeletal muscle mass (SMM) and body fat mass (BFM) to longitudinal growth among children and adolescents aged 6–11 years old.MethodsThis cohort study was conducted from the annual health examination between 2019 and 2020. Annual height gain and weight gain and changes in SMM and BFM were calculated and compared between sexes, different nutritional status, and growth curve shifting mode. Spearman analyses and multiple linear regression analysis were performed to identify the impact of SMM, BFM, or body mass index (BMI) on height gain.ResultsOf the 584 subjects, the annual height gains of boys (4.76 cm in the 6–9-year group and 4.63 cm in the 10–11-year group) were significantly lower than those of girls (5.48 and 5.74 cm, respectively). Spearman analysis showed that SMM gain and height gain were positively and significantly correlated in each examination of all children (r = 0.535 for boys and 0.734 for girls, p < 0.001). Conversely, BFM and height gains were negatively (r = -0.5240 for boys and -0.232 for girls, p < 0.001) correlated. Multiple linear regression analysis identified SMM gain as an independent predictor (95% CI: 1.20,1.44) of height gain after adjusting for age, gender, BMI, BFM gain, and percentage of body fat (PBF).ConclusionSMM gains, rather than BFM gains, were associated with height gains in children and adolescents aged 6–11 years. Monitoring SMM changes in routine healthcare might motivate children and adolescents to achieve dietary and exercise recommendations, thereby growing taller without gaining excessive weight

Effects of tin caulis dendrobium polysaccharide on the prevention of non-alcoholic fatty liver disease in rats

We aimed to investigate the effects and mechanisms of tin caulis dendrobium polysaccharide on body weight and non-alcoholic fatty liver disease in Sprague-Dawleg rats. The rats were fed a high-fat diet (HFD) with or without tin caulis dendrobium polysaccharide for eight weeks. Body weight, liver function tests, serum and liver lipids, liver morphology, and the expression of PPARγ and UCP2 were assessed. Rats fed HFD exhibited more obvious weight gain, increased serum, and hepatic lipids. But the more tin caulis dendrobium polysaccharide they fed, the lower increase in weight, serum, and hepatic lipids of rats were found. Furthermore, the expression of PPARγ and UCP2 decreased in rats fed tin caulis dendrobium polysaccharide compared with HFD-fed rats. Tin caulis dendrobium polysaccharide may downregulate the expression of PPARγ and UCP2 in liver and protect against body weight gain and liver fat deposition

Effects of tin caulis dendrobium polysaccharide on the prevention of non-alcoholic fatty liver disease in rats

ABSTRACT We aimed to investigate the effects and mechanisms of tin caulis dendrobium polysaccharide on body weight and non-alcoholic fatty liver disease in Sprague-Dawleg rats. The rats were fed a high-fat diet (HFD) with or without tin caulis dendrobium polysaccharide for eight weeks. Body weight, liver function tests, serum and liver lipids, liver morphology, and the expression of PPARγ and UCP2 were assessed. Rats fed HFD exhibited more obvious weight gain, increased serum, and hepatic lipids. But the more tin caulis dendrobium polysaccharide they fed, the lower increase in weight, serum, and hepatic lipids of rats were found. Furthermore, the expression of PPARγ and UCP2 decreased in rats fed tin caulis dendrobium polysaccharide compared with HFD-fed rats. Tin caulis dendrobium polysaccharide may downregulate the expression of PPARγ and UCP2 in liver and protect against body weight gain and liver fat deposition

Long noncoding RNA small nucleolar RNA host gene 12 promotes papillary thyroid carcinoma cell growth and invasion by targeting miR16-5p

Emerging evidence has shown that long noncoding RNA (lncRNA) plays an important role in various types of malignant cancer. Small nucleolar RNA host gene 12 (SNHG12) was found to be upregulated and to act as an oncogene in several cancers. However, the function and regulatory mechanism of SNHG12 remain unclear in papillary thyroid carcinoma (PTC). In this study, SNHG12 was found to be increased in PTC tissues and cell lines using quantitative real-time PCR. Knockdown of SNHG12 significantly inhibited PTC cell proliferation, migration and invasion and induced apoptosis in vitro. Mechanistic investigations revealed that SNHG12 functions as a competing endogenous RNA (ceRNA) to sponge miR-16-5p, which was downregulated in PTC tissues. In addition, rescue assays further confirmed that SNHG12 contributed to the progression of PTC through regulating miR-16-5p expression. These results indicated that SNHG12 might contribute to tumor progression in PTC by acting as a ceRNA to sponge miR-16-5p

HOXA13 promotes the proliferation, migration, and invasion of nasopharyngeal carcinoma HNE1 cells by upregulating the expression of Snail and MMP-2

Abstract Homeobox A13 (HOXA13) has been verified as an oncogen in some malignancies. However, its role in nasopharyngeal carcinoma (NPC) is still unclear. This study aims to explore the role of HOXA13 in NPC and its underlying mechanism. The mRNA expression of HOXA13 in NPC was obtained from the GSE53819 and GSE64634 datasets in the Gene Expression Omnibus (GEO) database. MTT, colony formation and transwell assays and xenograft tumour models were used to investigate the effects of HOXA13 on NPC HNE1 cells in vitro and in vivo. The expression of HOXA13, epithelial-mesenchymal transition-transcription factor (EMT-TF) Snail and matrix metalloproteinase 2 (MMP-2) was detected by immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. The results showed that HOXA13 was upregulated in NPC. Silencing HOXA13 suppressed the proliferation, migration, and invasion of HNE1 cells, which inhibited tumour growth, while overexpression of HOXA13 induced the opposite effects. In addition, the expression of Snail and MMP-2 at the transcriptional and protein levels was associated with the expression of HOXA13. In summary, our results suggest that HOXA13 plays a role as a cancer-promoting gene in NPC. The underlying mechanism may be related to the upregulation of Snail and MMP-2

Additional file 2: of Up-regulated NRIP2 in colorectal cancer initiating cells modulates the Wnt pathway by targeting RORβ

Analysis of the relationship between NRIP2, RORB and clinical parameters. (DOCX 1238 kb