Puromycin Sensitivity of Ribosomal Label after Incorporation of 14C-Labelled Amino Acids into Isolated Mitochondria from Neurospora crassa

Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the monosomes or the polysomes. The conclusion is drawn that isolated mitochondria under these conditions do not incorporate significant amounts of amino acids into proteins of their ribosomes

Results of Osteochondral Autologous Transplantation in the Knee

Repair of full thickness defects of articular cartilage in the knee is difficult but important to prevent progression to osteoarthritis. The purpose of this retrospective study was to evaluate the clinical results of Osteochondral Autograft Transplant System (OATS) treatment for articular defects of the knee

The Climatic Water Balance and Topography Control Spatial Patterns of Atmospheric Demand, Soil Moisture, and Shallow Subsurface Flow

Catchment hydrometeorology and the organization of shallow subsurface flow are key drivers of active contributing areas and streamflow generation. However, understanding how the climatic water balance and complex topography contribute to these processes from hillslope to catchment scales remains difficult. We compared time series of vapor pressure deficits and soil moisture to the climatic water balance and topographic variables across six zero-order catchments in the Lubrecht Experimental Forest (Montana, USA). We then evaluated how local hydrometeorology (volumetric water content and atmospheric vapor pressure deficit) affected the spatial occurrence of shallow subsurface flow. Generalized linear mixed model analysis revealed significant, temporally stable (monthly and seasonal average) patterns of hydrometeorology that can be predicted by the topographic wetness index and the dynamic climatic water deficit (CWD = potential evapotranspiration - actual evapotranspiration). Intracatchment patterns were significantly correlated to the topographic wetness index, while intercatchment patterns were correlated to spatiotemporal variance in the CWD during each time period. Spatial patterns of shallow subsurface flow were related to the hydrometeorological conditions of the site. We observed persistent shallow subsurface flow in convergent hillslope positions, except when a catchment was positioned in locations with high CWDs (low elevations and southerly aspects). Alternatively, we observed persistent subsurface flow across all hillslope positions (even 70-m upslope from the hollow) when catchments were positioned in locations with especially low CWDs (northerly aspects and high elevations). These results highlight the importance of considering the superposition of the catchment-scale climatic water balance and hillslope-scale topography when characterizing hydrometeorology and shallow subsurface flow dynamics.USDA NIFA McIntire Stennis award [233327]; NSF grants [DEB-1457749, DEB-1457720]; NASA applied science program Wildland Fire award [NNH11ZDA001N-FIRES]; NSF EPSCoR through the Montana Institute on Ecosystems6 month embargo; published online: 19 February 2019This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

Investigating the Stratigraphy of an Alluvial Aquifer Using Crosswell Seismic Traveltime Tomography

In this study, we investigate the use of crosswell P-wave seismic tomography to obtain spatially extensive information about subsurface sedimentary architecture and heterogeneity in alluvial aquifers. Our field site was a research wellfield in an unconfined aquifer near Boise, Idaho. The aquifer consists of a ~ 20-m-thick sequence of alluvial cobble- and-sand deposits, which have been subdivided into five stratigraphic units based on neutron porosity logs, grainsize analysis, and radar reflection data. We collected crosswell and borehole-to-surface seismic data in wells 17.1 m apart. We carefully considered the impact of well deviation, data quality control, and the choice of inversion parameters. Our linearized inverse routine had a curved-ray forward model and used different grids for forward modeling and inversion. An analysis of the model covariance and resolution matrices showed that the velocity models had an uncertainty of \u3c10 m\u3e/s, a vertical resolution of ~ 1 m, and a horizontal resolution of ~ 5 m. The velocity in the saturated zone varied between 2100 m/s and 2700 m/s. Inclusion of the borehole-to-surface data eliminated the Xshaped pattern that is a common artifact in crosswell tomography, and the increased angular coverage also improved the accuracy of the model near the top of the tomogram. The final velocity model is consistent with previous stratigraphic analyses of the site, although the locations of some of the unit boundaries differ by as much as 2 m in places. The results of this study demonstrate that seismic tomography can be used to image the sedimentary architecture of unconsolidated alluvial aquifers, even when the lithologic contrasts between units are subtle

The Ursinus Weekly, January 11, 1965

Football players receive awards at banquet: Tony Motto gets All-ECAC • Ursinus to send delegation to Model UN • Weekly names Sam Walker sports editor • Faculty Forum to present concert, lecture recital: 20th century American music, subject • Three placed in teaching positions • Pre-meds to hear two Sacred Heart pathologists • World\u27s Fair invites college talent to perform • Pi Nu Epsilon, music fraternity, initiates eight • Norristown man named to vacancy in Treas. Office • Good band, decorations add to great TG dance • The best TV show in the world • Editorial: Our large small college • Bob Dylan: Alone and indifferent • J. D. Salinger writes for The Ursinus Weekly • Anthem, a warning to society • The Doanes report on teaching in the South • Bears drop two, F&M-Swarthmore; Troster\u27s thirty points rock PMC • Matmen drop opener to Del. • Soccer team puts three on All-MAC • Y Commission sponsors film on discrimination • Greek gleanings • Letters to the editor • Kaffee Klatsch debates Negro block bustinghttps://digitalcommons.ursinus.edu/weekly/1238/thumbnail.jp

The Ursinus Weekly, November 9, 1964

Senior Ball, Friday, offers Camelot, Al Raymond band: Lord and Lady, permanent class officers to be announced • Graduate awards available from science foundation • Miller and Zucker review election returns, meaning • Museum director on American art to be Forum highlight: Dr. Turner to emphasize the 20th century • William Shaffer, vice-president UC Board of Directors, dies • Pancoast in State House to seek greater local power • Faculty action penalizes 4 with fines, demerits • Curtain Club selects cast for Winnie the Pooh production, schedules December showing • English Club to meet tonight • Editorial: Altruism at Ursinus • Life in the cow palace; An eyewitness account • Kaffee Klatsch hosts large crowd to discuss rights • A girl\u27s life at Ursinus: 1906 • Spotlight: UC abroad • Advice column • Bears belt Haverford 19-6 at Haverford homecoming: Tony Motto scores two touchdowns • Soccermen defeat LaSalle 5-2 • Hockey team wins biggest: W.C., best ever, falls 1-0; West Chester places 5 on all-stars, Ursinus team effort proves supreme • Wrestling to start Tuesday • U.C. men\u27s mooning team suffers setback at hands of faculty • 200 dance to Okie Duke at Cafe Montmarte • Greek gleaningshttps://digitalcommons.ursinus.edu/weekly/1233/thumbnail.jp

Parental expressed emotion toward children: prediction from early family functioning

Expressed emotion (EE), an index of family member criticism and emotional overinvolvement, predicts outcome among adults and children with mental disorders. However, limited research exists on factors contributing to EE. Aims of the current study were to (a) examine EE in mothers of young children; (b) assess relationships between demographic factors and EE in a diverse sample; and (c) investigate whether family stress and functioning, including quality of marital relationship, life events, maternal stress, and family environment, predict EE. In the current study, 276 mothers completed questionnaires when their children were between 1 and 3 years of age and a measure of EE when their children were in kindergarten. Results indicated that family expressiveness was the most consistent predictor of EE. Further research seems warranted to better delineate associations between family functioning and EE. Keywords: predictors of EE in families, family environment expressiveness, expressed emotion and the family environment, family stress and expresed emotio

Runoff response of a small agricultural basin in the argentine Pampas considering connectivity aspects

Our manuscript analyses the surface runoff variability, and its controlling factors in a small basin with gentle slopes, at the headwaters of a flat catchment, to improve the knowledge of the hydrology of plain areas under agriculture. We study runoff, rainfall and antecedent conditions in the argentine Pampas region. We use correlations, regressions and quantitative and qualitative descriptive information of the system: erosion signs, ground cover by crops, groundwater depth data and temporal changes in the drainage network, to discuss and understand the complexity of the runoff process by frameworks to study (dis)connectivity. The analysis of 56 events evidenced a nonlinear rainfall–runoff relationship. In contrast with other works, we identified clear upper limit events, under which hydrological responses emerge, as a result of combinations of antecedent wetness, rainfall erosivity, ground cover and preferential drainage paths. We separated the nonlinear rainfall–runoff response in three linear relationships according to differences in antecedent wetness conditions. We found differences in runoff responses under wet and dry antecedent conditions, but complex responses under medium antecedent conditions. The analyses of the inputs, the structural and the functional elements of the (dis)connectivity frameworks, were key in the understanding of the temporal changes of runoff, and its complex responses. Temporal coincidences of connectivity components and their feedbacks appear to be strongly associated with the runoff dynamics. High-magnitude hydrological responses occur with complete coincidences, while partial coincidences between the components reduce connectivity and low magnitude and/or heterogeneous responses prevail. Thus, these analyses suggest that runoff is controlled by (dis)connectivity in this basin with gentle slopes. Our work contributes to the understanding of the process of surface runoff in the context of humid flatlands under agricultural land use, by the identification of the complex combinations of factors which regulate/control the (dis)connectivity that helps to interpret the nonlinearities of runoff.Fil: Ares, María Guadalupe. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Hidrología de Llanuras "Dr. Eduardo Jorge Usunoff". - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Instituto de Hidrología de Llanuras "Dr. Eduardo Jorge Usunoff". - Universidad Nacional del Centro de la Provincia de Buenos Aires. Instituto de Hidrología de Llanuras "Dr. Eduardo Jorge Usunoff"; ArgentinaFil: Varni, Marcelo Raúl. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Hidrología de Llanuras "Dr. Eduardo Jorge Usunoff". - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Instituto de Hidrología de Llanuras "Dr. Eduardo Jorge Usunoff". - Universidad Nacional del Centro de la Provincia de Buenos Aires. Instituto de Hidrología de Llanuras "Dr. Eduardo Jorge Usunoff"; ArgentinaFil: Chagas, Celio Ignacio. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Ingenieria Agricola y Uso de la Tierra. Cátedra de Manejo y Conservación de Suelo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Modeling subsurface hydrology in floodplains

Evans would also like to acknowledge the generous funding provided by the Natural Environment Research Council (NERC) and the University of St Andrews 600 Fund, without which this work would not have been possible. Singer was supported by funding from NSF EAR #700555. The data output files are hosted online by the NERC Environmental Information Data Centre [Evans et al., 2018].Soil-moisture patterns in floodplains are highly dynamic, owing to the complex relationships between soil properties, climatic conditions at the surface, and the position of the water table. Given this complexity, along with climate change scenarios in many regions, there is a need for a model to investigate the implications of different conditions on water availability to riparian vegetation. We present a model, HaughFlow, which is able to predict coupled water movement in the vadose and phreatic zones of hydraulically connected floodplains. Model output was calibrated and evaluated at 6 sites in Australia to identify key patterns in subsurface hydrology. This study identifies the importance of the capillary fringe in vadose zone hydrology due to its water storage capacity and creation of conductive pathways. Following peaks in water table elevation, water can be stored in the capillary fringe for up to months (depending on the soil properties). This water can provide a critical resource for vegetation that is unable to access the water table. When water table peaks coincide with heavy rainfall events, the capillary fringe can support saturation of the entire soil profile. HaughFlow is used to investigate the water availability to riparian vegetation, producing daily output of water content in the soil over decadal time periods within different depth ranges. These outputs can be summarised to support scientific investigations of plant-water relations, as well as in management applications.Publisher PDFPeer reviewe