Wind, jet, hybrid corona and hard X-ray flares: multiwavelength evolution of GRO J1655-40 during the 2005 outburst rise

We have investigated the complex multiwavelength evolution of GRO J1655-40 during the rise of its 2005 outburst. We detected two hard X-ray flares, the first one during the transition from the soft state to the ultra-soft state, and the second one in the ultra-soft state. The first X-ray flare coincided with an optically thin radio flare. We also observed a hint of increased radio emission during the second X-ray flare. To explain the hard flares without invoking a secondary emission component, we fit the entire data set with the eqpair model. This single, hybrid Comptonization model sufficiently fits the data even during the hard X-ray flares if we allow reflection fractions greater than unity. In this case, the hard X-ray flares correspond to a Comptonizing corona dominated by non-thermal electrons. The fits also require absorption features in the soft and ultra-soft state which are likely due to a wind. In this work we show that the wind and the optically thin radio flare co-exist. Finally, we have also investigated the radio to optical spectral energy distribution, tracking the radio spectral evolution through the quenching of the compact jet and rise of the optically thin flare, and interpreted all data using state transition models.Comment: 16 pages, 11 figure

In vitro transfection of HeLa cells with temperature sensitive polycationic copolymers

Cataloged from PDF version of article.In this study, we investigated different types of polyethyleneimine (PEI) and their block copolymers with N-isopropylacrylamide (NIPA) as temperature-sensitive polycationic non-viral vectors for transfection of HeLa cells in cell culture media. First carboxyl-terminated poly(NIPA) was synthesized and then copolymerized with PEIs branched or linear and with two different molecular weights (2 and 25 kDa). Addition of PEI units to the poly(NIPA) chains increased the LCST values up to body temperature. Zeta potentials of the copolymers were significantly lower than the corresponding PEI homopolymers. A green fluorescent protein expressing plasmid was used as a model. Complexes of this plasmid both with PEIs and their copolymers were formed. The zeta potentials of these complexes were between -3.1 and +21.3. Higher values were observed for the complexes prepared with branched and higher molecular weight PEIs. Copolymerization caused a profound decrease in the positive charges. Particle sizes of the complexes were in the range of 190-1235 nm. Using high polymer/plasmid ratios caused aggregation. The smallest complexes were obtained with the copolymer prepared with branched PEI with 25-kDa molecular weight. Copolymers were able to squeeze plasmid DNA more at the body temperature. Cytotoxicity was observed with PEIs especially with the branched higher molecular weights. Copolymerization reduced the cytotoxicity. The best in vitro DNA uptake efficiency (70%) was achieved with the complex prepared with poly(NIPA)/PEI25B. However, poly(NIPA)/PEI25L was the most successful vector for an effective gene expression without any significant toxicity. © 2004 Elsevier B.V. All rights reserved

Complete Multiwavelength Evolution of Galactic Black Hole Transients During Outburst Decay II: Compact Jets and X-ray Variability Properties

We investigated the relation between compact jet emission and X-ray variability properties of all black hole transients with multiwavelength coverage during their outburst decays. We studied the evolution of all power spectral components (including low frequency quasi-periodic oscillations), and related this evolution to changes in jet properties tracked by radio and infrared observations. We grouped sources according to their tracks in radio/X-ray luminosity relation, and show that the standards show stronger broadband X-ray variability than outliers at a given X-ray luminosity when the compact jet turned on. This trend is consistent with the internal shock model and can be important for the understanding of the presence of tracks in the radio/X-ray luminosity relation. We also observed that the total and the QPO rms amplitudes increase together during the earlier part of the outburst decay, but after the compact jet turns either the QPO disappears or its rms amplitude decreases significantly while the total rms amplitudes remain high. We discuss these results with a scenario including a variable corona and a non-variable disk with a mechanism for the QPO separate from the mechanism that create broad components. Finally, we evaluated the timing predictions of the magnetically dominated accretion flow model which can explain the presence of tracks in the radio/X-ray luminosity relation.Comment: Accepted for publication by Ap

Multiwavelength observations of the black hole transient XTE J1752-223 during its 2010 outburst decay

Galactic black hole transients show many interesting phenomena during outburst decays. We present simultaneous X-ray (RXTE, Swift, and INTEGRAL), and optical/near-infrared (O/NIR) observations (SMARTS), of the X-ray transient, XTE J1752-223 during its outburst decay in 2010. The multi- wavelength observations of 150 days in 2010 cover the transition from soft to hard spectral state. The evolution of ATCA/VLBI radio observations are shown to confirm the compact jet appearance. The source shows flares in O/NIR during changes in X-ray and radio properties. One of those flares is bright and long, and starts about 20 days after the transition in timing. Other, smaller flares occur along with the transition in timing and increase in power-law flux, and also right after the detection of the core with VLBI. Furthermore, using the simultaneous broadband X-ray spectra including IN- TEGRAL, we found that a high energy cut-off is necessary with a folding energy at around 250 keV around the time that the compact jet is forming. The broad band spectrum can also be fitted equally well with a Comptonization model. In addition, using photoelectric absorption edges in the XMM– Newton RGS X-ray spectra and the extinction of red clump giants in the direction of the source, we found a lower limit on the distance of > 5 kpc

Multiwavelength observations of the black hole transient Swift J1745-26 during the outburst decay

We characterized the broad-band X-ray spectra of Swift J1745-26 during the decay of the 2013 outburst using INTEGRAL ISGRI, JEM-X and Swift XRT. The X-ray evolution is compared to the evolution in optical and radio. We fit the X- ray spectra with phenomenological and Comptonization models. We discuss possible scenarios for the physical origin of a ~50 day flare observed both in optical and X- rays ~170 days after the peak of the outburst. We conclude that it is a result of enhanced mass accretion in response to an earlier heating event. We characterized the evolution in the hard X-ray band and showed that for the joint ISGRI-XRT fits, the e-folding energy decreased from 350 keV to 130 keV, while the energy where the exponential cut-off starts increased from 75 keV to 112 keV as the decay progressed.We investigated the claim that high energy cut-offs disappear with the compact jet turning on during outburst decays, and showed that spectra taken with HEXTE on RXTE provide insufficient quality to characterize cut-offs during the decay for typical hard X-ray fluxes. Long INTEGRAL monitoring observations are required to understand the relation between the compact jet formation and hard X-ray behavior. We found that for the entire decay (including the flare), the X-ray spectra are consistent with thermal Comptonization, but a jet synchrotron origin cannot be ruled out.Comment: Accepted for publication by MNRA

Numerical analysis of heat transfer in cooling of fish packages

The present work aims at finding the optimal finite difference scheme for the solution of problems involving pure heat transfer from the surface of solids suddenly exposed to a cooling environment. Fish samples in the form of infinite slab were considered, and a generalized mathematical model was constructed in dimensionless form. A more representative and accurate set of experimental data is chosen from the experimental work for comparison with the numerical results and evaluation of numerical schemes. In the analysis, a fully explicit finite difference scheme, an implicit finite difference scheme and different combination schemes with varying values of weighting factor are thoroughly studied. The characteristic dimension (half thickness of the slab) is divided into a number of divisions; n = 5, 10, 20, 30, 40, 50 and 100, respectively. All the possible options of the Fourier number increments are taken one by one to give the best convergence and minimal truncation error. The simplest explicit finite difference scheme with n = 10 and Fourier number increments one sixth of the square of the space division size appears to be highly reliable and accurate for such applications

The Okavango; a river supporting its people, environment and economic development

The Okavango basin comprises the Cuito and Cubango active catchment areas in Angola, in addition to the Kavango–Okavango non-active catchment in northern Namibia and Botswana. The Okavango River water and its ecosystem resources are critically important sources of livelihoods for people in the basin. Pressures from livelihoods and development are already impacting on the environment. These pressures may increase in the future due to the rapid increase in population, the peace process and associated resettlement activities in Angola, and major development initiatives in Botswana and Namibia. For instance, possible future increase in water abstraction from the Okavango River may affect the long-term environmental sustainability of the Okavango Delta by minimizing channel shifting and thereby reducing spatial biodiversity. The paper argues that while conservation of the natural environment is critical, the pressing development needs must be recognized. The reduction of poverty within the basin should be addressed in order to alleviate adverse effects on the environment. The paper recommends that the development of sustainable tourism and community-based natural resource management initiatives may be appropriate strategies for reaching the Millennium Development Goals of poverty alleviation and achievement of environmental sustainability in the Okavango Basin. These initiatives have a comparative advantage in this area as demonstrated by the performance of the existing projects

Structure-based design and synthesis of antiparasitic pyrrolopyrimidines targeting pteridine reductase 1

The treatment of Human African Trypanosomiasis remains a major unmet health need in sub-Saharan Africa. Approaches involving new molecular targets are important and pteridine reductase 1 (PTR1), an enzyme that reduces dihydrobiopterin in Trypanosoma spp. has been identified as a candidate target and it has been shown previously that substituted pyrrolo[2,3-d]pyrimidines are inhibitors of PTR1 from T. brucei (J. Med. Chem. 2010, 53, 221-229). In this study, 61 new pyrrolo[2,3-d]pyrimidines have been prepared, designed with input from new crystal structures of 23 of these compounds complexed with PTR1, and evaluated in screens for enzyme inhibitory activity against PTR1 and in vitro antitrypanosomal activity. 8 compounds were sufficiently active in both screens to take forward to in vivo evaluation. Thus although evidence for trypanocidal activity in a stage I disease model in mice was obtained, the compounds were too toxic to mice for further development

Acupuncture Point Localization Varies Among Acupuncturists

Background: Studies assessing the point-specific effect of acupuncture or the characteristics of acupuncture points (APs) tend to yield inconclusive results. In order to identify a possible confounding factor, we aimed to examine the variability in AP localization by means of a survey. Material and Methods: Attendees of the 14th ICMART (International Council of Medical Acupuncture and Related Techniques) congress as well as DAGfA (German Medical Society of Acupuncture) lecturers and students were asked to locate and mark the APs LI 10 and TH 5 on a research assistant's arm. Identified points were transferred into a coordinate system, and the respective bivariate distribution function was calculated. Additionally, participants filled out a questionnaire about their acupuncture education and experience, the acupuncture style and point localization techniques used most frequently, and their estimation of the size of an AP. Results: The areas of the ellipses, theoretically containing 95% of AP localizations, varied between 44.49 and 5.18 cm(2). The largest distance between 2 identified points was 8.45 cm for LI 10 and 5.3 cm for TH 5. Apart from being trained at the same school, no other factor could be identified that determined the variability in AP localization. Conclusion: Our results indicate that congruity of AP localization among experienced acupuncturists might be low. Although there are some limitations to our results, this possible bias should be taken into account when conducting acupuncture trials and interpreting results of previous acupuncture studies