709 research outputs found
Probing the Subcellular Localization of Hopanoid Lipids in Bacteria Using NanoSIMS
The organization of lipids within biological membranes is poorly understood. Some studies have suggested lipids group
into microdomains within cells, but the evidence remains controversial due to non-native imaging techniques. A recently developed NanoSIMS technique indicated that sphingolipids group into microdomains within membranes of human fibroblast cells. We extended this NanoSIMS approach to study the localization of hopanoid lipids in bacterial cells by developing a stable isotope labeling method to directly detect subcellular localization of specific lipids in bacteria with ca. 60 nm resolution. Because of the relatively small size of bacterial cells and the relative abundance of hopanoid lipids in membranes, we employed a primary ^2H-label to maximize our limit of detection. This approach permitted the analysis of multiple stable isotope labels within the same sample, enabling visualization of subcellular lipid microdomains within different cell types using a secondary label to mark the growing end of the cell. Using this technique, we demonstrate subcellular localization of hopanoid lipids within alpha-proteobacterial and cyanobacterial cells. Further, we provide evidence of hopanoid lipid domains in between cells of the filamentous cyanobacterium Nostoc punctiforme. More broadly, our method provides a means to image lipid microdomains in a wide range of cell types and test hypotheses for their functions in membranes
Suppression of the ferromagnetic state in LaCoO3 films by rhombohedral distortion
Epitaxially strained LaCoO3 (LCO) thin films were grown with different film
thickness, t, on (001) oriented (LaAlO3)0.3(SrAl0.5Ta0.5O3)0.7 (LSAT)
substrates. After initial pseudomorphic growth the films start to relieve their
strain partly by the formation of periodic nano-twins with twin planes
predominantly along the direction. Nano-twinning occurs already at the
initial stage of growth, albeit in a more moderate way. Pseudomorphic grains,
on the other hand, still grow up to a thickness of at least several tenths of
nanometers. The twinning is attributed to the symmetry lowering of the
epitaxially strained pseudo-tetragonal structure towards the relaxed
rhombohedral structure of bulk LCO. However, the unit-cell volume of the
pseudo-tetragonal structure is found to be nearly constant over a very large
range of t. Only films with t > 130 nm show a significant relaxation of the
lattice parameters towards values comparable to those of bulk LCO.Comment: 31 pages, 10 figure
MetaboLab - advanced NMR data processing and analysis for metabolomics
Background\ud
Despite wide-spread use of Nuclear Magnetic Resonance (NMR) in metabolomics for the analysis of biological samples there is a lack of graphically driven, publicly available software to process large one and two-dimensional NMR data sets for statistical analysis.\ud
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Results\ud
Here we present MetaboLab, a MATLAB based software package that facilitates NMR data processing by providing automated algorithms for processing series of spectra in a reproducible fashion. A graphical user interface provides easy access to all steps of data processing via a script builder to generate MATLAB scripts, providing an option to alter code manually. The analysis of two-dimensional spectra (1H,13C-HSQC spectra) is facilitated by the use of a spectral library derived from publicly available databases which can be extended readily. The software allows to display specific metabolites in small regions of interest where signals can be picked. To facilitate the analysis of series of two-dimensional spectra, different spectra can be overlaid and assignments can be transferred between spectra. The software includes mechanisms to account for overlapping signals by highlighting neighboring and ambiguous assignments.\ud
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Conclusions\ud
The MetaboLab software is an integrated software package for NMR data processing and analysis, closely linked to the previously developed NMRLab software. It includes tools for batch processing and gives access to a wealth of algorithms available in the MATLAB framework. Algorithms within MetaboLab help to optimize the flow of metabolomics data preparation for statistical analysis. The combination of an intuitive graphical user interface along with advanced data processing algorithms facilitates the use of MetaboLab in a broader metabolomics context.\ud
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Search for neutrino oscillations on a long base-line at the CHOOZ nuclear power station
This final article about the CHOOZ experiment presents a complete description
of the electron antineutrino source and detector, the calibration methods and
stability checks, the event reconstruction procedures and the Monte Carlo
simulation. The data analysis, systematic effects and the methods used to reach
our conclusions are fully discussed. Some new remarks are presented on the
deduction of the confidence limits and on the correct treatment of systematic
errors.Comment: 41 pages, 59 figures, Latex file, accepted for publication by
Eur.Phys.J.
Initial Results from the CHOOZ Long Baseline Reactor Neutrino Oscillation Experiment
Initial results are presented from CHOOZ, a long-baseline reactor-neutrino
vacuum-oscillation experiment. Electron antineutrinos were detected by a liquid
scintillation calorimeter located at a distance of about 1 km. The detector was
constructed in a tunnel protected from cosmic rays by a 300 MWE rock
overburden. This massive shielding strongly reduced potentially troublesome
backgrounds due to cosmic-ray muons, leading to a background rate of about one
event per day, more than an order of magnitude smaller than the observed
neutrino signal. From the statistical agreement between detected and expected
neutrino event rates, we find (at 90% confidence level) no evidence for
neutrino oscillations in the electron antineutrino disappearance mode for the
parameter region given approximately by deltam**2 > 0.9 10**(-3) eV**2 for
maximum mixing and (sin(2 theta)**2) > 0.18 for large deltam**2.Comment: 13 pages, Latex, submitted to Physics Letters
Limits on Neutrino Oscillations from the CHOOZ Experiment
We present new results based on the entire CHOOZ data sample. We find (at 90%
confidence level) no evidence for neutrino oscillations in the anti_nue
disappearance mode, for the parameter region given by approximately Delta m**2
> 7 x 10**-4 eV^2 for maximum mixing, and sin**2(2 theta) = 0.10 for large
Delta m**2. Lower sensitivity results, based only on the comparison of the
positron spectra from the two different-distance nuclear reactors, are also
presented; these are independent of the absolute normalization of the anti_nue
flux, the cross section, the number of target protons and the detector
efficiencies.Comment: 19 pages, 11 figures, Latex fil
Measurement of the transverse target and beam-target asymmetries in meson photoproduction at MAMI
We present new data for the transverse target asymmetry T and the very first
data for the beam-target asymmetry F in the
reaction up to a center-of-mass energy of W=1.9 GeV. The data were obtained
with the Crystal-Ball/TAPS detector setup at the Glasgow tagged photon facility
of the Mainz Microtron MAMI. All existing model predictions fail to reproduce
the new data indicating a significant impact on our understanding of the
underlying dynamics of meson photoproduction. The peculiar nodal
structure observed in existing T data close to threshold is not confirmed.Comment: 5 pages, 3 figures, accepted for publication in PR
T and F asymmetries in π0 photoproduction on the proton
The γp→π0p reaction was studied at laboratory photon energies from 425 to 1445 MeV with a transversely polarized target and a longitudinally polarized beam. The beam-target asymmetry F was measured for the first time and new high precision data for the target asymmetry T were obtained. The experiment was performed at the photon tagging facility of the Mainz Microtron (MAMI) using the Crystal Ball and TAPS photon spectrometers. The polarized cross sections were expanded in terms of associated Legendre functions and compared to recent predictions from several partial-wave analyses. The impact of the new data on our understanding of the underlying partial-wave amplitudes and baryon resonance contributions is discussed
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