Practitioners\u27 Perpectives on the Impact of Migratory Separation on Attachment Among Southeast Asian Clients: An Exploratory Study

Evidenced by the multitude of literature across disciplines, attachment theory has ignited one of today’s most prolific lines of research. Attachment Theory’s core themes of security, separation, and loss apply well into the common experience of immigrants and refugees who have dealt with these issues in their immigration experience. Consistently, studies have shown that separation and traumas before, during, and after the migration journey have been identified as potential predictors for serious psychological distress and mental health problems among immigrants and refugees. Given the profound implications from recent studies, there exists limited research on how migratory separation affects attachment. Thus, the goal of this exploratory research was to examine the variables of separation and attachment by using both quantitative and qualitative research methodologies to investigate mental health professionals’ perspectives on the impact of separation due to immigration on attachment related issues among Southeast Asian clients. Further, to gather mental health implications and recommendations on culturally sensitive practices. The sample was comprised of fourteen mental health professionals who work predominantly with Southeast Asian immigrant and refugee populations. The participants provided insightful observations on the complex relationship between attachment and separation due to immigration among Southeast Asian diaspora populations. Most notably, they described common challenges, Eastern cultural lens of attachment, and offered intervention recommendations for working with this client populations. This study brings awareness to mental health professionals everywhere of the multifaceted effects separation can have on immigrant and refugees’ well-being and to call for appropriate interventions to assure effective, ethical, and adequate service for this growing populations in our nations and in the Twin Cities metro area

A time-of-flight mass spectrometer suitable for ionospheric composition investigations

Time-of-flight spectrometer for examining ionospheric ion density in D and E region

What Could the Big6 Strategy Do to Students’ English and Information Literacies?

This study was aimed to investigate what the Big6 strategy could do to the development of both students’ proficiency in English literacy and information literacy. To achieve the purpose, an experimental study with time series design was conducted. Forty-five students of a private secondary school in Palembang were randomly selected for intervention based on their levels of reading. To collect the data, both English literacy and information literacy tests were given to the students. The results of paired-sample-t test analysis show that both students’ English and information literacies improved significantly. However, the students’ level of English Literacy was only a little bit above average and their information literacy was proficient. When regression analysis was used, only students’ speaking skill of English Literacy and the ethics aspect of information literacy contributed the least to the total achievement of each variable. These imply that the Big6 strategy is able to make a difference in students’ English and information literacies. It also deserves to be used in ELT classrooms in the future for students to enhance their English literacy including their oral expression skill and to familiarize themselves with ethical aspect of information literacy from their puberty

DOUBLE-STRAND BREAK REPAIR PATHWAYS IN DNA STRUCTURE-INDUCED GENETIC INSTABILITY

Genetic instability in mammalian cells can occur by many different mechanisms. In the absence of exogenous sources of DNA damage, the DNA structure itself has been implicated in genetic instability. When the canonical B-DNA helix is naturally altered to form a non-canonical DNA structure such as a Z-DNA or H-DNA, this can lead to genetic instability in the form of DNA double-strand breaks (DSBs) (1, 2). Our laboratory found that the stability of these non-B DNA structures was different in mammals versus Escherichia coli (E.coli) bacteria (1, 2). One explanation for the difference between these species may be a result of how DSBs are repaired within each species. Non-homologous end-joining (NHEJ) is primed to repair DSBs in mammalian cells, while bacteria that lack NHEJ (such as E.coli), utilize homologous recombination (HR) to repair DSBs. To investigate the role of the error-prone NHEJ repair pathway in DNA structure-induced genetic instability, E.coli cells were modified to express genes to allow for a functional NHEJ system under different HR backgrounds. The Mycobacterium tuberculosis NHEJ sufficient system is composed of Ku and Ligase D (LigD) (3). These inducible NHEJ components were expressed individually and together in E.coli cells, with or without functional HR (RecA/RecB), and the Z-DNA and H-DNA-induced mutations were characterized. The Z-DNA structure gave rise to higher mutation frequencies compared to the controls, regardless of the DSB repair pathway(s) available; however, the type of mutants produced after repair was greatly dictated on the available DSB repair system, indicated by the shift from 2% large-scale deletions in the total mutant population to 24% large-scale deletions when NHEJ was present (4). This suggests that NHEJ has a role in the large deletions induced by Z-DNA-forming sequences. H-DNA structure, however, did not exhibit an increase in mutagenesis in the newly engineered E.coli environment, suggesting the involvement of other factors in regulating H-DNA formation/stability in bacterial cells. Accurate repair by established DNA DSB repair pathways is essential to maintain the stability of eukaryotic and prokaryotic genomes and our results suggest that an error-prone NHEJ pathway was involved in non-B DNA structure-induced mutagenesis in both prokaryotes and eukaryotes

Bleeding Severity in Percutaneous Coronary Intervention (PCI) and Its Impact on Short-Term Clinical Outcomes

Bleeding severity in patients undergoing percutaneous coronary intervention (PCI), defined by the Bleeding Academic Research Consortium (BARC), portends adverse prognosis. We analysed data from 37,866 Australian patients undergoing PCI enrolled in the Victorian Cardiac Outcomes Registry (VCOR), and investigated the association between increasing BARC severity and in-hospital and 30-day major adverse cardiac and cerebrovascular events (MACCE) (a composite of mortality, myocardial infarction, stent thrombosis, target vessel revascularisation, or stroke). Independent predictors associated with major bleeding (BARC groups 3&5), and MACCE were also assessed. There was a stepwise increase in in-hospital and 30-day MACCE with greater severity of bleeding. Independent predictors of bleeding included female sex (Odds Ratio (OR) 1.34), age (OR 1.02), fibrinolytic therapy (OR 1.77), femoral access (OR 1.51), and ticagrelor (OR 1.42), all significant at the p < 0.001 level. Following adjustment of clinically important variables, BARC 3&5 bleeds (OR 4.37) were still predictive of cumulative in-hospital and 30-day MACCE. In conclusion, major bleeding is an uncommon but potentially fatal PCI complication and was independently associated with greater MACCE rates. Efforts to mitigate the occurrence of bleeding, including radial access and judicious use of potent antiplatelet therapies, may ameliorate the risk of short-term adverse clinical outcomes

Methane and nitrous oxide fluxes across an elevation gradient in the tropical Peruvian Andes

Peer reviewedPublisher PD