Análisis de linajes maternos y paternos de bovinos criollo del Centro de Ecología Aaplicada Simón I. Patiño - Bolivia

Se determinaron los linajes maternos y paternos de 33 bovinos Criollo (27 hembras y 6 machos) del Centro de Ecología Aplicada Simón I. Patiño (Ceasip) mediante marcadores genéticos del ADN genómico, mitocondrial y del cromosoma Y. El ADN genómico se extrajo utilizando el kit Wizard ® Genomic Purification. Los linajes maternos se determinaron mediante secuenciación del ADN mitocondrial (región control D-loop) y los linajes paternos se determinaron analizando siete marcadores genéticos del cromosoma Y, dos SNP (Polimorfismo de Nucleótido Simple) y cinco microsatélites (Secuencias Repetidas en Tándem). La diversidad genética se estimó tipificando 18 microsatélites. Se analizaron los datos con MStools, GenePop y Arlequin. La secuenciación de D-loop mitocondrial permitió detectar seis linajes maternos, que incluían cuatro haplotipos mitocondriales de origen europeo y dos africanos. A través del análisis de los marcadores del cromosoma Y se determinaron tres linajes paternos, dos taurinos y uno cebuino. En el hato del Ceasip, la diversidad alélica (na) fue de 6.11, mientras que la heterocigosidad esperada (He) fue de 0.70 y la observada (Ho) fue de 0.68. Los valores de diversidad genética observada en los bovinos del Ceasip son similares a los estimados para la mayoría de los biotipos del Criollo boliviano (na Yacumeño= 6.82; na Saavedreño= 5.95), siendo los valores promedios para el ganado Criollo boliviano analizados anteriormente de na= 6.39, He= 0.72 y Ho= 0.65. Los análisis de Componentes Principales y de distancia genética mostraron que sería factible intercambiar material genético entre las poblaciones Criollo bolivianas sin pérdida significativa de su diversidad genética.Fil: Pereira, J. A. C.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Peña, S.. Centro de Ecología Aplicada Simón I. Patiño; BoliviaFil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria ; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Loza, A. J.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Posik, Diego Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Instituto de Genética Veterinaria ; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Baudoin, M.. Centro de Ecología Aplicada Simón I. Patiño; BoliviaFil: Bomblat, C.. Centro de Ecología Aplicada Simón I. Patiño; Bolivi

Association between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle

<p>Abstract</p> <p>Background</p> <p>In bovines, there are significant differences within and among beef breeds in the time when bulls reach puberty. Although the timing of puberty is likely to be a multigenic trait, previous studies indicate that there may also be single genes that exert major effects on the timing of puberty within the general population. Despite its economic importance, there are not many SNPs or genetic markers associated with the age of puberty in male cattle. In the present work, we selected three candidate genes, <it>GNRHR</it>, <it>LHR </it>and <it>IGF1</it>, and associated their polymorphisms with the age of puberty in Angus male cattle.</p> <p>Results</p> <p>After weaning, 276 Angus males were measured every month for weight (W), scrotal circumference (SC), sperm concentration (C) and percentage of motility (M). A total of 4 SNPs, two within <it>GNRHR</it>, one in <it>LHR </it>and one in <it>IGF1 </it>were genotyped using the pyrosequencing technique. <it>IGF1-SnaBI SNP </it>was significant associated (P < 0.01) with age at SC 28 cm, but it were not associated with age at M 10% and C 50 million. Genotype <it>CC </it>exhibited an average age at SC 28 cm of 7 and 11 days higher than <it>CT </it>(p = 0.037) and <it>TT </it>(p = 0.012), respectively. This SNP explained 1.5% of the genetic variance of age of puberty at SC28. <it>LHR-I499L</it>, <it>GNRHR-SNP5 </it>and <it>GNRHR-SNP6 </it>were not associated with any of the measurements. However, <it>GNRHR </it>haplotypes showed a suggestive association with age at SC 28 cm.</p> <p>Conclusions</p> <p>The findings presented here could support the hypothesis that <it>IGF1 </it>is a regulator of the arrival to puberty in male calves and is involved in the events that precede and initiate puberty in bull calves. Given that most studies in cattle, as well as in other mammals, were done in female, the present results are the first evidence of markers associated with age at puberty in male cattle.</p

Genetic characterization of the creole cattle population from los valles (Santa Cruz, Bolivia) through autosomal, mitochondrial and y-chromosome genetic markers

In Bolivia, there are currently three types of Creole cattle: Altiplano, Valleys, and Plains. The cattle of the Valleys comprise isolated populations which have been little studied so far. The objective of the present study was to perform the genetic characterization of the Creole cattle from the Valleys of Santa Cruz. We analyzed 17 autosomal microsatellites (STRs), 5 STRs and one indel of the Y chromosome and a fragment of the D-Loop region of mitochondrial DNA. DNA was extracted from 98 Creole animals belonging to: 25 from the population of the Valleys of Santa Cruz, 35 from Yacumeño Creole, 17 from Saavedreño Creole, and 21 from Creole of the Centro de Ecología Aplicada Simón I. Patiño (CEASIP). The 17 autosomal loci studied were polymorphic. The average number of alleles per locus was 5.18, ranging from two to 10. The Heterozygosis values observed in the studied STRs varied between 0.083 and 0.898, resulting in an average heterozygosity of 0.664. The analysis of the Y chromosome markers allowed the detection of two haplotypes, one from B.taurus origin (Y2 haplogroup; - Val1) and other of zebu origin (Y3 haplogroup). The analysis of the maternal lineages detected ten mitochondrial haplotypes, three belonging to the European haplogroup T3 and seven to the African haplogroup T1. The analysis of genetic distances and the principal component analysis, based on microsatellite data, showed that the population studied exhibited a larger divergence with respect to the other Bolivian Creole cattle populations. Maternal genetic composition showed a mixed European and African origin. Finally, the analysis of the Y chromosome, as well as the Structure analysis showed that the population of Creole cattle of the Valley showed introgression of Zebu genes. The study can be the starting point to create conservation programs for the Creole cattle from the valleys of Santa Cruz, which is an important animal resource that must be conserved.Instituto de Genética Veterinari

Recent patents for detecting the species of origin in animal feedstuff, and raw and processed meat products

The value of the traceability and labeling of food is attributable to two main aspects: health safety and/or product or process certification. The identification of the species related to meat production is still a major concern for economic, religious and health reasons. Many approaches and technologies have been used for species identification in animal feedstuff and food. The early methods for meat products identification include physical, anatomical, histological and chemical. Since 1970, a variety of methods were developed, these include electrophoresis (i.e. isoelectrofocusing), chromatography (i.e. HPLC), immunological techniques (i.e. ELISA), Nuclear Magnetic Resonance, Mass Spectrometry and PCR (DNA and RNA based methods). The recent patents on species detection in animal feedstuffs, raw meat and meat processed products, listed in this work, are mainly based on monoclonal antibodies and PCR, especially RT-PCR. The new developments under research are looking for more sensible, specific, less time consuming and quantitatively detection methods, which can be used in highly processed or heated treated meat food.Fil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Posik, Diego M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Ripoli, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Falomir Lockhart, Agustin Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Peral Garcia, Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Genética Veterinaria "ingeniero Fernando Noel Dulout"; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentin

Origin of cuban creole cattle inferred by patri- and matrilineages

Cattle was absent from America before the discovery. Initially, bovine were brought to Greater Antilles (La Española, Puerto Rico, Jamaica and Cuba islands), and in the course of a few years, they were taken from Caribbean islands to the rest of Latin America. Nowadays, Cuban Creole cattle population is about 1300 heads, mainly located in the eastern region of the island. With the aim of analyzing the maternal origin of Cuban Creole cattle and detect possible contemporaneous, male mediated, gene flow, a 240 pb fragment of mitochondrial D-loop (mtDNA) and five micro- satellites of Y chromosome (BTY) were studied in 36 dams and 21 sires, respectively. Genetic diversity was evaluated through number of haplotypes, mean number of pairwise differences and nucleotide diversity. The phylogenetic analysis was performed using a median joining. A total of 15 mtDNA haplotypes were detected in the studied population (10 from the European haplogroup T3, 3 from the African T1, 1 from the Nearern East T2, and 1 ambiguous T1-T3). The number of polymorphic sites, the mean nucleotide diversity, and the mean number of pairwise differences were 23, 0.014 and 3.36, respectively. Two patrilinages were detected, both belonging to the Y3 Zebu haplogroup. In conclusion, Cuban Creole cattle population had a mtDNA haplotypic composition similar to the observed in Creole and Mediterranean breeds, what is in concordance with its historical origin. Y chromosome analysis evidenced a male mediated process of zebu introgression.Antes de descubrimiento, no existían bovinos en América. Los primeros, fueron introducidos en la Antillas Mayores (La Española, Puerto Rico, Jamaica y Cuba), y desde allí trasladados al resto de Latinoamérica. Actualmente, existen en Cuba alrededor de 1300 bovinos Criollos, concentrados principalmente en la región oriental. Con el objetivo de analizar el origen materno de esta raza y detectar eventos contemporáneos de flujo génico por vía paterna, se analizó un fragmento de 240 pb del D-loop mitocondrial (mtADN) y 5 microsatélites del cromosoma Y (BTY), en 36 hembras y 21 machos respectivamente. La diversidad genética se estimó mediante el número de haplotipos, el número de sitios polimórficos, el número de dife- rencias nucleotídicas entre pares de secuencias y el índice de diversidad nucleotídica, mientras que el análisis filogenético se realizó utilizando el método de median joining network. Dicho análisis permitió detectar 15 haplotipos mitocondriales (10 del haplogrupo europeo T3, 3 del africano T1, 1 del cercano oriente T2 y 1 ambiguo T1-T3) y 3 haplotipos en el BTY, ambos del haplogrupo cebuíno Y3. En el mtADN se detectaron 23 sitios polimórficos con una diversidad nucleotídica de 0,014 y 3,36 dife- rencias medias entre pares de secuencias. En conclusión, la población de bovinos Criollos Cuba- nos presentó una composición haplotípica mitocondrial comparable a la de otras razas crio- llas y mediterráneas, hecho que concuerda con su origen histórico. El BTY evidenció altos niveles de introgresion paterna de genes del zebú

Maternal and paternal lineage analysis in creole cattle from the Center of Applied Ecology Simón I. Patiño - Bolivia

Se determinaron los linajes maternos y paternos de 33 bovinos Criollo (27 hembras y 6 machos) del Centro de Ecología Aplicada Simón I. Patiño (Ceasip) mediante marcadores genéticos del ADN genómico, mitocondrial y del cromosoma Y. El ADN genómico se extrajo utilizando el kit Wizard® Genomic Purification. Los linajes maternos se determinaron mediante secuenciación del ADN mitocondrial (región control D-loop) y los linajes paternos se determinaron analizando siete marcadores genéticos del cromosoma Y, dos SNP (Polimorfismo de Nucleótido Simple) y cinco microsatélites (Secuencias Repetidas en Tándem). La diversidad genética se estimó tipificando 18 microsatélites. Se analizaron los datos con MStools, GenePop y Arlequin. La secuenciación de D-loop mitocondrial permitió detectar seis linajes maternos, que incluían cuatro haplotipos mitocondriales de origen europeo y dos africanos. A través del análisis de los marcadores del cromosoma Y se determinaron tres linajes paternos, dos taurinos y uno cebuino. En el hato del Ceasip, la diversidad alélica (na) fue de 6.11, mientras que la heterocigosidad esperada (He) fue de 0.70 y la observada (Ho) fue de 0.68. Los valores de diversidad genética observada en los bovinos del Ceasip son similares a los estimados para la mayoría de los biotipos del Criollo boliviano (na Yacumeño= 6.82; na Saavedreño= 5.95), siendo los valores promedios para el ganado Criollo boliviano analizados anteriormente de na= 6.39, He= 0.72 y Ho= 0.65. Los análisis de Componentes Principales y de distancia genética mostraron que sería factible intercambiar material genético entre las poblaciones Criollo bolivianas sin pérdida significativa de su diversidad genética.Paternal and maternal lineages of 33 Creole bovines (27 cows and 6 bulls) from the Center of Applied Ecology Simón I. Patiño (Ceasip) were determined by nuclear, mitochondrial, and Y chromosome DNA markers. DNA was extracted with the Wizard® Genomic Purification Kit. Maternal lineages were identified by mitochondrial DNA sequencing (D-loop region). Seven Y chromosome markers - two Simple Nucleotide Polymorphisms (SNPs) and five microsatellites (tandem repeat sequences) - were used to determine paternal lineages. Genetic diversity was estimated with 18 microsatellites markers. Data were analyzed with MStools, GenePop, and Arlequin software. Two European and two African mitochondrial haplotypes were identified in the maternal lineages. Two taurine and one cebuine paternal lineages were detected. Allelic diversity (na) was 6.11, expected heterozygosity (He) was 0.70, and observed heterozygosity (Ho) was 0.68. These values were similar to average values of Yacumeño and Saavedreño Creole (na= 6.39, He = 0.72, and Ho = 0.65). Allelic diversity in Ceasip bovines (na= 6.11) was also similar to Yacumeño Creole (6.82) and Saavedreño Creole (5.95) biotypes. Cluster and Principal Component analyses indicate that genetic material could be exchanged between populations without significant diversity loss.Facultad de Ciencias VeterinariasInstituto de Genética Veterinari

Maternal and paternal lineage analysis in creole cattle from the Center of Applied Ecology Simón I. Patiño - Bolivia

Se determinaron los linajes maternos y paternos de 33 bovinos Criollo (27 hembras y 6 machos) del Centro de Ecología Aplicada Simón I. Patiño (Ceasip) mediante marcadores genéticos del ADN genómico, mitocondrial y del cromosoma Y. El ADN genómico se extrajo utilizando el kit Wizard® Genomic Purification. Los linajes maternos se determinaron mediante secuenciación del ADN mitocondrial (región control D-loop) y los linajes paternos se determinaron analizando siete marcadores genéticos del cromosoma Y, dos SNP (Polimorfismo de Nucleótido Simple) y cinco microsatélites (Secuencias Repetidas en Tándem). La diversidad genética se estimó tipificando 18 microsatélites. Se analizaron los datos con MStools, GenePop y Arlequin. La secuenciación de D-loop mitocondrial permitió detectar seis linajes maternos, que incluían cuatro haplotipos mitocondriales de origen europeo y dos africanos. A través del análisis de los marcadores del cromosoma Y se determinaron tres linajes paternos, dos taurinos y uno cebuino. En el hato del Ceasip, la diversidad alélica (na) fue de 6.11, mientras que la heterocigosidad esperada (He) fue de 0.70 y la observada (Ho) fue de 0.68. Los valores de diversidad genética observada en los bovinos del Ceasip son similares a los estimados para la mayoría de los biotipos del Criollo boliviano (na Yacumeño= 6.82; na Saavedreño= 5.95), siendo los valores promedios para el ganado Criollo boliviano analizados anteriormente de na= 6.39, He= 0.72 y Ho= 0.65. Los análisis de Componentes Principales y de distancia genética mostraron que sería factible intercambiar material genético entre las poblaciones Criollo bolivianas sin pérdida significativa de su diversidad genética.Paternal and maternal lineages of 33 Creole bovines (27 cows and 6 bulls) from the Center of Applied Ecology Simón I. Patiño (Ceasip) were determined by nuclear, mitochondrial, and Y chromosome DNA markers. DNA was extracted with the Wizard® Genomic Purification Kit. Maternal lineages were identified by mitochondrial DNA sequencing (D-loop region). Seven Y chromosome markers - two Simple Nucleotide Polymorphisms (SNPs) and five microsatellites (tandem repeat sequences) - were used to determine paternal lineages. Genetic diversity was estimated with 18 microsatellites markers. Data were analyzed with MStools, GenePop, and Arlequin software. Two European and two African mitochondrial haplotypes were identified in the maternal lineages. Two taurine and one cebuine paternal lineages were detected. Allelic diversity (na) was 6.11, expected heterozygosity (He) was 0.70, and observed heterozygosity (Ho) was 0.68. These values were similar to average values of Yacumeño and Saavedreño Creole (na= 6.39, He = 0.72, and Ho = 0.65). Allelic diversity in Ceasip bovines (na= 6.11) was also similar to Yacumeño Creole (6.82) and Saavedreño Creole (5.95) biotypes. Cluster and Principal Component analyses indicate that genetic material could be exchanged between populations without significant diversity loss.Facultad de Ciencias VeterinariasInstituto de Genética Veterinari

Maternal and paternal lineage analysis in creole cattle from the Center of Applied Ecology Simón I. Patiño - Bolivia

Se determinaron los linajes maternos y paternos de 33 bovinos Criollo (27 hembras y 6 machos) del Centro de Ecología Aplicada Simón I. Patiño (Ceasip) mediante marcadores genéticos del ADN genómico, mitocondrial y del cromosoma Y. El ADN genómico se extrajo utilizando el kit Wizard® Genomic Purification. Los linajes maternos se determinaron mediante secuenciación del ADN mitocondrial (región control D-loop) y los linajes paternos se determinaron analizando siete marcadores genéticos del cromosoma Y, dos SNP (Polimorfismo de Nucleótido Simple) y cinco microsatélites (Secuencias Repetidas en Tándem). La diversidad genética se estimó tipificando 18 microsatélites. Se analizaron los datos con MStools, GenePop y Arlequin. La secuenciación de D-loop mitocondrial permitió detectar seis linajes maternos, que incluían cuatro haplotipos mitocondriales de origen europeo y dos africanos. A través del análisis de los marcadores del cromosoma Y se determinaron tres linajes paternos, dos taurinos y uno cebuino. En el hato del Ceasip, la diversidad alélica (na) fue de 6.11, mientras que la heterocigosidad esperada (He) fue de 0.70 y la observada (Ho) fue de 0.68. Los valores de diversidad genética observada en los bovinos del Ceasip son similares a los estimados para la mayoría de los biotipos del Criollo boliviano (na Yacumeño= 6.82; na Saavedreño= 5.95), siendo los valores promedios para el ganado Criollo boliviano analizados anteriormente de na= 6.39, He= 0.72 y Ho= 0.65. Los análisis de Componentes Principales y de distancia genética mostraron que sería factible intercambiar material genético entre las poblaciones Criollo bolivianas sin pérdida significativa de su diversidad genética.Paternal and maternal lineages of 33 Creole bovines (27 cows and 6 bulls) from the Center of Applied Ecology Simón I. Patiño (Ceasip) were determined by nuclear, mitochondrial, and Y chromosome DNA markers. DNA was extracted with the Wizard® Genomic Purification Kit. Maternal lineages were identified by mitochondrial DNA sequencing (D-loop region). Seven Y chromosome markers - two Simple Nucleotide Polymorphisms (SNPs) and five microsatellites (tandem repeat sequences) - were used to determine paternal lineages. Genetic diversity was estimated with 18 microsatellites markers. Data were analyzed with MStools, GenePop, and Arlequin software. Two European and two African mitochondrial haplotypes were identified in the maternal lineages. Two taurine and one cebuine paternal lineages were detected. Allelic diversity (na) was 6.11, expected heterozygosity (He) was 0.70, and observed heterozygosity (Ho) was 0.68. These values were similar to average values of Yacumeño and Saavedreño Creole (na= 6.39, He = 0.72, and Ho = 0.65). Allelic diversity in Ceasip bovines (na= 6.11) was also similar to Yacumeño Creole (6.82) and Saavedreño Creole (5.95) biotypes. Cluster and Principal Component analyses indicate that genetic material could be exchanged between populations without significant diversity loss.Facultad de Ciencias VeterinariasInstituto de Genética Veterinari

Genetic characterization of the creole cattle population from los valles (Santa Cruz, Bolivia) through autosomal, mitochondrial and y-chromosome genetic markers

In Bolivia, there are currently three types of Creole cattle: Altiplano, Valleys, and Plains. The cattle of the Valleys comprise isolated populations which have been little studied so far. The objective of the present study was to perform the genetic characterization of the Creole cattle from the Valleys of Santa Cruz. We analyzed 17 autosomal microsatellites (STRs), 5 STRs and one indel of the Y chromosome and a fragment of the D-Loop region of mitochondrial DNA. DNA was extracted from 98 Creole animals belonging to: 25 from the population of the Valleys of Santa Cruz, 35 from Yacumeño Creole, 17 from Saavedreño Creole, and 21 from Creole of the Centro de Ecología Aplicada Simón I. Patiño (CEASIP). The 17 autosomal loci studied were polymorphic. The average number of alleles per locus was 5.18, ranging from two to 10. The Heterozygosis values observed in the studied STRs varied between 0.083 and 0.898, resulting in an average heterozygosity of 0.664. The analysis of the Y chromosome markers allowed the detection of two haplotypes, one from B.taurus origin (Y2 haplogroup; - Val1) and other of zebu origin (Y3 haplogroup). The analysis of the maternal lineages detected ten mitochondrial haplotypes, three belonging to the European haplogroup T3 and seven to the African haplogroup T1. The analysis of genetic distances and the principal component analysis, based on microsatellite data, showed that the population studied exhibited a larger divergence with respect to the other Bolivian Creole cattle populations. Maternal genetic composition showed a mixed European and African origin. Finally, the analysis of the Y chromosome, as well as the Structure analysis showed that the population of Creole cattle of the Valley showed introgression of Zebu genes. The study can be the starting point to create conservation programs for the Creole cattle from the valleys of Santa Cruz, which is an important animal resource that must be conserved.Fil: Pereira, J. A. C.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Gutierrez, L.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Siancas, M.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Orellana, J.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Loza, A. J.. Universidad Autónoma Gabriel René Moreno; BoliviaFil: Rogberg Muñoz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; ArgentinaFil: Castillo, Nadia Sabiela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; ArgentinaFil: Posik, Diego Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; ArgentinaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria ; Argentin

Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd

During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals. In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with that provided by STRs. Thirty-six closely related Angus cattle were genotyped for 18 STRs and 116 SNPs. Cumulative SNPs exclusion power values (Q) for paternity and sample matching probability (MP) yielded values greater than 0.9998 and 4.32E-42, respectively. Generally 2-3 SNPs per STR were needed to obtain an equivalent Q value. The MP showed that 24 SNPs were equivalent to the ISAG (International Society for Animal Genetics) minimal recommended set of 12 STRs (MP ~ 10-11). These results provide valuable genetic data that support the consensus SNP panel for bovine genetic identification developed by the Parentage Recording Working Group of ICAR (International Committee for Animal Recording)