Orthographic knowledge and use in children with dyslexia

PURPOSE: To investigate how children with dyslexia use the phonographemic correspondence rule in their writing regardless of context and how they identify and analyze their writing mistakes according to the variables: frequency of occurrence of the linguistic item and school grade. METHODS: The writing errors observed on a task of dictation of high- and low-frequency words and pseudowords were analyzes and compared. Fifty-six students from 1st to 4th grades of private elementary schools were evaluated: 28 with dyslexia (research group), and 28 without writing complaints or deficits (comparison group), paired by age, gender and grade. The reasons for right and wrong answers were taped and compared. RESULTS: The statistic analysis showed that students with dyslexia performed worse than the comparison group. Moreover, there was a decrease in the number of errors with the progression of schooling, especially in high frequency words. Students with dyslexia showed more difficulty to analyze the linguistic items and recognize their correct writing. CONCLUSION: Children with dyslexia had more difficulties in writing and analyzing linguistic items of transparent orthography. The effect of lexical frequency facilitated error identification. The occurrence of errors decreased with schooling progression and varied according to linguistic item.OBJETIVO: Investigar como escolares com Transtorno Específico de Leitura (TLE) aplicam em suas escritas a regra de correspondência fono-grafêmica independente do contexto e como identificam e analisam os erros que produzem na escrita, segundo as variáveis: freqüência de aparecimento do item lingüístico e a série do escolar. MÉTODOS: Foram analisados e comparados os erros de escrita obtida a partir de ditado de palavras de alta e baixa freqüência e pseudopalavras, de escolares de 1ª a 4ª série da rede particular de ensino fundamental. Avaliaram-se 56 escolares: 28 com TLE constituíram o grupo pesquisa e 28 sem queixas ou alterações, pareados por idade, sexo e série, o grupo de comparação. Justificativas dos erros e acertos de escrita foram gravadas e comparadas. RESULTADOS: Os escolares do grupo pesquisa mostraram pior desempenho quando comparados com o de comparação. Além disso, observou-se diminuição dos erros, conforme a progressão da escolaridade, principalmente na escrita de palavras de alta freqüência. Os escolares do grupo pesquisa apresentaram maior dificuldade em analisar os itens lingüísticos escritos e reconhecer as escritas corretas. CONCLUSÕES: As crianças com transtorno específico de leitura apresentaram maior dificuldade em escrever e analisar a escrita de ortografia transparente. O efeito da freqüência do item lingüístico facilitou a identificação do erro. Nesse grupo de escolares os erros diminuíram com a progressão das séries e variaram conforme o item lingüístico.Universidade Federal de São Paulo (UNIFESP)Universidade Federal de São Paulo (UNIFESP) Departamento de FonoaudiologiaUNIFESP, Depto. de FonoaudiologiaSciEL

Adaptação cultural e análise da consistência interna do instrumento MISSCARE para uso no Brasil

OBJECTIVE: The aims of this methodological research were to culturally adapt the MISSCARE Survey instrument to Brazil and analyze the internal consistency of the adapted version. METHOD: The instrument consists of 41 items, presented in two parts. Part A contains 24 items listing elements of missed nursing care. Part B is comprised of 17 items, related to the reasons for not delivering care. The research received ethics committee approval and was undertaken in two phases. The first was the cultural adaptation process, in which a committee of five experts verified the face and content validity, in compliance with the steps recommended in the literature. The second was aimed at analyzing the internal consistency of the instrument, involving 60 nursing team professionals at a public teaching hospital. RESULTS: According to the experts, the instrument demonstrated face and content validity. Cronbach's alpha coefficients for parts A and B surpassed 0.70 and were considered appropriate. CONCLUSION: The adapted version of the MISSCARE Survey demonstrated satisfactory face validity and internal consistency for the study sample.OBJETIVOS: Los objetivos de este estudio metodológico fueron efectuar la adaptación cultural del instrumento MISSCARE para Brasil y analizar la consistencia interna de la versión adaptada. MÉTODO: El instrumento contiene 41 ítem, presentados en dos partes. La parte A contiene 24 ítem, referentes a los elementos de los cuidados de enfermería omitidos, y la parte B 17 ítem, relacionados a los motivos para no prestar los cuidados. La investigación, con aprobación del comité de ética, fue efectuada en dos fases. La primera fue el proceso de adaptación cultural, que verificó la validez aparente y de contenido, llevado a cabo por un comité de cinco jueces según los pasos recomendados por la literatura. El objetivo de la segunda fue analizar la consistencia interna del instrumento con 60 profesionales del equipo de enfermería de un hospital público de enseñanza universitaria. RESULTADO: El instrumento demostró validez aparente y de contenido según los especialistas. Los valores del coeficiente alfa de Cronbach, calculados para las partes A y B del instrumento, fueron superiores a 0.70, siendo considerados adecuados. CONCLUSIÓN: La versión adaptada del MISSCARE mostró consistencia interna satisfactoria para la muestra estudiada.OBJETIVO: Este estudo metodológico teve como objetivos realizar a adaptação cultural do instrumento MISSCARE para o Brasil e analisar a consistência interna da versão adaptada. MÉTODO: O instrumento possui 41 itens apresentados em duas partes. A parte A contém 24 itens referentes aos elementos dos cuidados de enfermagem omitidos e a parte B, 17 itens relacionados às razões para a não prestação dos cuidados. A pesquisa, aprovada pelo comitê de ética, foi realizada em duas fases. A primeira consistiu no processo de adaptação cultural, que verificou a validade de face e de conteúdo, realizada por um comitê de cinco juízes conforme os passos preconizados pela literatura. A segunda visou analisar a consistência interna do instrumento com 60 profissionais da equipe de enfermagem de um hospital público de ensino universitário. RESULTADO: O instrumento demonstrou validade de face e conteúdo na opinião dos especialistas. Os valores do coeficiente alfa de Cronbach, obtidos para as partes A e B do instrumento, foram acima de 0,70, sendo considerados adequados. CONCLUSÃO: A versão adaptada do MISSCARE mostrou consistência interna satisfatória para a amostra estudada

Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera

The aim of this work was to select among 300 strains of Bacillus thuringiensis those which are simultaneously effective against larvae of Spodoptera frugiperda J.E. Smith and Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), Anthonomus grandis Boheman (Coleoptera: Curculionidae), Aedes aegypti Linnaeus and Culex quinquefasciatus Say (Diptera: Culicidae). Two strains of B. thuringiensis were selected, S234 and S997, which presented activity against those three insect orders. Both strains were characterized by morphological, biochemical and molecular methods. They have presented two main proteins with 130 and 65 kDa, polimerase chain reaction products with expected sizes for detection of the genes cry1Aa, cry1Ab, cry1Ac, cry1B and cry2 and bipiramidal, cubical and spherical crystals.O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), Anthonomus grandis Boheman (Coleoptera: Curculionidae), Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae). Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e cry2 e cristais bipiramidais, cubóides e esféricos

Avaliação da virulência em hamsters (Mesocricetus auratus) de estirpes de Mycobacterium avium presentes na população de suínos do sul do Brasil

The finding of four clusters of M. avium (PIG-A, B, C and D), typed by the IS1245-RFLP method, infecting the swine population of the south region of Brazil, the possible existence of virulence differences among them, the role of the virulence in the transmission mechanisms of infections and the existence of reasonable doubts regarding the importance of horizontal transmission for swine micobacteriosis, the virulence of these four strains of M. avium were compared. Bacteria from each cluster were inoculated in 48 hamsters by intra-peritoneal route. On the 2nd, 13th, 26th, and 40th days after inoculation, (T1 to T4), 12 animals of each cluster were sacrificed with vapors of ethyl ether and the bacteria were quantified in the liver, spleen and lung. Results were expressed as cfu/g of organ. The presence of the strains was verified in the blood and histological exams were also accomplished. The four strains induced granulomatous lesions in the liver and spleen since 2 days after inoculation and were disseminated to the lungs through the blood stream. The cfu counts from spleen were always bigger them that obtained from liver and lungs. Differences among strains were observed through the analysis of cfu counts from spleen (T1: p; PIG-A>; PIG-D>; PIG-C.Tendo sido comprovada a existência de quatro famílias molecularmente distintas de M. avium (PIG-A, B, C e D) circulando em suínos da região sul do Brasil, e havendo dúvidas a respeito da importância da transmissão horizontal como mecanismo de manutenção da doença, o presente teve por objetivo estudar a virulência dessas estirpes, informação importante para o aperfeiçoamento dos métodos de controle. Uma estirpe representante de cada família foi inoculada pela via intra-peritoneal em 48 hamsters com uma dose de 30.000 U.F.C. por animal. Após 2, 13, 26 e 40 dias da inoculação (T1 a T4), 12 hamsters inoculados de cada família foram anestesiados, sacrificados e os agentes foram quantificados no fígado, baço e pulmão. Os resultados foram expressos em número de U.F.C./g de órgão. A presença das estirpes foi pesquisada no sangue e também foram realizados exames histológicos. As estirpes PIG-A, B, C e D induziram a formação de lesões granulomatosas no fígado e baço a partir do segundo dia pós-inoculação e disseminaram-se pela via hemática, alcançando os pulmões. O baço sempre apresentou maiores contagens de U.F.C., seguido pelo figado e pulmões. Diferenças entre as estirpes foram constatadas através de análises das contagens de U.F.C de baço (T1: p; PIG-A>; PIG-D>; PIG-C

Estudo Vida e Saúde em Pomerode (SHIP-Brazil): objetivos, aspectos metodológicos e resultados descritivos

Background Few studies compared populations with similar genetic and culture background on different continents with standardized methods. Objective To describe methodological issues of the Study of Health in Pomerode - SHIP-Brazil and some characteristics of the participants of the baseline examination. Design and Setting Prospective, population-based cohort study of a representative sample of residents (aged 20 to 79 years) of Pomerode, Santa Catarina, Brazil. Methods Data for the baseline survey (from 2014 to 2018) were collected through interviews and medical examinations, including socio-demographic and lifestyle information, clinical and subclinical conditions, oral and mental health, among others. Biosamples (blood, urine, stool, and saliva) were collected and stored. Methods of data collection and quality control are described. Preliminary descriptive statistics were performed. Results The response rate was 67.6% (n=2,488 individuals). The Kappa test-retest of some variables varied from 0.54 to 1.0. German culture participants are older (46.5 vs 38.7 years), self-declared white (97.3% vs 82.1%), more frequently never smokers (71.4% vs 66.9%) but had higher risk of consuming alcohol (16.9% vs 13.4%) compared to participants with non-German background. Germans were taller (169 cm vs 166 cm), had greater abdominal circumference among men (101.9 cm vs 97.3 cm). Furthermore, they reported more multimorbidity (56.7% vs 43.6%) , had more arterial hypertension (30.7% vs 18.5%), but less depression (15.4% vs 19,1%) than non-Germans. Conclusions The interaction of genetic and social/environmental issues should be examined to understand the role of risk factors on clinical conditions observed.Introdução Poucos estudos compararam populações com histórico genético e cultural semelhante em diferentes continentes com métodos padronizados. Objetivos Descrever questões metodológicas do estudo de “Vida e Saúde em Pomerode - SHIP-Brazil” e algumas características dos participantes do exame inicial do estudo. Desenho de estudo e local Estudo de coorte prospectivo de base populacional em amostra representativa de moradores (20 a 79 anos) de Pomerode, Santa Catarina. Métodos As informações para a linha de base (de 2014 a 2018) foram coletadas por meio de entrevistas e exames médicos, incluindo dados sociodemográficos, de estilo de vida, condições clínicas e subclínicas, saúde bucal e mental, entre outros. Amostras biológicas (sangue, urina, fezes e saliva) foram coletadas e armazenadas. A coleta de dados e o controle de qualidade foram descritos. Foram realizadas análises descritivas preliminares. Resultados A taxa de resposta foi de 67,6% (n=2.488 indivíduos). O Kappa teste-reteste de algumas variáveis variou  de 0,54 a 1,0. Os participantes de cultura alemã são mais velhos (46,5 vs 38,7 anos ), autodeclarados brancos (97,3% vs 82,1%), com menor número de fumantes (71,4% vs 66,9%), mas tiveram maior risco de consumir álcool (16,9% vs 13,4%), eram mais altos (169 cm vs 166 cm), tinham maior circunferência abdominal entre os homens (101,9 cm vs 97,3 cm) em comparação com participantes “não-alemães”. Pessoas de cultura alemã relataram mais multimorbidade (56,7% vs 43,6%), apresentavam mais hipertensão arterial (30,7% vs 18,5%), mas menos depressão (15,4% vs 19,1%). Conclusões A interação genética e social/ambiental devem ser examinadas para melhor entender o papel desses fatores de risco nas condições clínicas observadas

Anti-inflammatory and redox-protective activities of citronellal

The anti-inflammatory and redox protective effects of the citronellal (CT) were evaluated using in vivo and in vitro tests. Intraperitoneal (i.p.) administration of CT (50, 100, and 200 mg/kg) inhibited (p < 0.05) the carrageenan-induced leukocyte migration to the peritoneal cavity. Additionally, the carrageenan- and arachidonic acid-induced rat hind paw edema was significantly inhibited (p < 0.05) by i.p. administration of 100 and 200 mg/kg of the compound. When the redox activity was evaluated, CT (200 mg/kg) significantly reduced hepatic lipoperoxidation (p < 0.001), as well as oxidation of plasmatic (p < 0.05) and hepatic (p < 0.01) proteins. The results of the present study support the hypothesis that CT possesses anti-inflammatory and redox protective activities. It is suggested that its effects are associated with the inhibition of the enzymes in the arachidonic acid pathway, which prevent cell migration by inhibiting leukotriene production, edema formation and the increase of reactive oxygen species in tissues. Therefore, CT is of potential benefit to manage inflammatory disorders and correlated damages caused by oxidant agents

Cultural adaptation and internal consistency analysis of the MISSCARE Survey for use in Brazil

OBJECTIVE: The aims of this methodological research were to culturally adapt the MISSCARE Survey instrument to Brazil and analyze the internal consistency of the adapted version. METHOD: The instrument consists of 41 items, presented in two parts. Part A contains 24 items listing elements of missed nursing care. Part B is comprised of 17 items, related to the reasons for not delivering care. The research received ethics committee approval and was undertaken in two phases. The first was the cultural adaptation process, in which a committee of five experts verified the face and content validity, in compliance with the steps recommended in the literature. The second was aimed at analyzing the internal consistency of the instrument, involving 60 nursing team professionals at a public teaching hospital. RESULTS: According to the experts, the instrument demonstrated face and content validity. Cronbach's alpha coefficients for parts A and B surpassed 0.70 and were considered appropriate. CONCLUSION: The adapted version of the MISSCARE Survey demonstrated satisfactory face validity and internal consistency for the study sample

Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis

Interleukin (IL)-15 is a pleiotropic cytokine that regulates the proliferation and survival of many cell types. IL-15 is produced by monocytes and macrophages against infectious agents and plays a pivotal role in innate and adaptive immune responses. This study analyzed the effect of IL-15 on fungicidal activity, oxidative metabolism and cytokine production by human monocytes challenged in vitro with Paracoccidioides brasiliensis (Pb18), the agent of paracoccidioidomycosis. Peripheral blood monocytes were pre-incubated with IL-15 and then challenged with Pb18. Fungicidal activity was assessed by viable fungi recovery from cultures after plating on brain-heart infusion-agar. Superoxide anion (O2-), hydrogen peroxide (H2O2), tumour necrosis factor-alpha (TNF-&#945;), IL-6, IL-15 and IL-10 production by monocytes were also determined. IL-15 enhanced fungicidal activity against Pb18 in a dose-dependent pattern. This effect was abrogated by addition of anti-IL-15 monoclonal antibody. A significant stimulatory effect of IL-15 on O2- and H2O2 release suggests that fungicidal activity was dependent on the activation of oxidative metabolism. Pre-treatment of monocytes with IL-15 induced significantly higher levels of TNF-&#945;, IL-10 and IL-15 production by cells challenged with the fungus. These results suggest a modulatory effect of IL-15 on pro and anti-inflammatory cytokine production, oxidative metabolism and fungicidal activity of monocytes during Pb18 infection

COVID-19, FAKE NEWS E VACINAÇÃO: OS DESAFIOS ENFRENTADOS NA SAÚDE PÚBLICA

The Covid-19 pandemic caused the disease to spread rapidly across all continents and spread various positive and negative information, which circulated or circulated on the internet, presenting different content. The objective of this study is to inform the population about the Fake News approach and the spread of misinformation and the challenges that public health faces. Studies were carried out from secondary sources, through bibliographic research in scientific databases, in the time window from 2021 to 2023, with the most recent and compatible articles prevailing. Eleven complete and published articles were found, related to vaccination against Covid-19, Fake News associated with the vaccination process and the influence on the population's adherence to immunizations in the context of public health. The Covid-19 pandemic, which was characterized by the acute respiratory syndrome popularly known as SARS-CoV-2, which resulted in several deaths due to lack of vaccination and also generated the infodemic and misinformation pandemic, where society began to discredit science and credibility it had.continentes e propagou diversas informações positivas e negativas, que circulam ou circularam na internet apresentando conteúdos diversos. O Objetivo deste estudo é informar a população sobre a abordagem de Fake News e a propagação da desinformação e os desafios que saúde pública enfrenta. Foram realizados estudos a partir de fontes secundárias, por meio da pesquisa bibliográfica em bancos de dados científicos, na janela temporal de 2021 a 2023, prevalecendo os artigos mais recentes e compatível. Encontrou-se onze artigos completos e publicados, relacionados à vacinação contra a Covid-19, Fake News associadas ao processo vacinal e a influência na adesão da população aos imunizantes no contexto de saúde pública. A pandemia de Covid-19 que foi caracterizada pela síndrome respiratória aguda popularmente conhecida como SARS-CoV-2, que obtive diversas mortes por falta de vacinação e também gerou a pandemia da infodemia e desinformação, onde a sociedade começou a desacreditar nas ciências e na credibilidade que a mesma tinha