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Metagenomic Analysis of Bacterial Community in the Gut of Blister Beetle Mylabris pustulata Thunberg

Blister beetles are an important object of study in the field of agriculture and health. Despite being known to be important for the host animal, the gut bacteria of blister beetles have not been investigated deeply. This study aimed to provide the first initial description of the gut bacterial community of Mylabris pustulata as one species of blister beetles, based on a culture-independent technique. Adult blister beetles of the same colony were sampled and confirmed as Mylabris pustulata Thunberg. The gut content was used in high throughput sequencing, targeting the V3-V4 regions of 16S rRNA gene, and in the cultivation of resident bacteria. The results showed that higher bacterial richness was present in the gut communities when compared to the grown bacterial culture. Proteobacteria was confirmed as the most abundant phylum in the gut of M. pustulata, whereby most reads belonged to the class of Gammaproteobacteria. The dominant bacterial genera were determined as Enterobacter, Acinetobacter, Enterococcus, Klebsiella, and Pseudomonas. In addition, our cultivation attempts led to successful isolation of members of Klebsiella and Enterococcus. The subject of this study could be explored further to find the potential roles of these microbiota in the gut of the specific beetles and their bioprospects

Endovascular treatment of large and wide aortic neck: case report and literature review

Large (24\u201334 mm) and wide ( 6535 mm) aortic necks are a contraindication to endovascular aneurysm repair (EVAR). A 63-year-old man, unfit for conventional surgery, presented a 79 mm abdominal aortic aneurysm with 36.5 mm aortic neck and a 62 mm right common iliac artery aneurysm. He was treated endovascularly with standard commercially available stent-graft using the so-called \u2018funnel technique\u2019; by placing a thoracic stent-graft inside a bifurcated device to achieve proximal sealing. The completion angiography and the 6 months follow-up with computed tomography showed no stent-graft migration, limb occlusion or endoleak. The literature review reported 179 cases of large aortic neck managed with EVAR, all cases treated with standard devices. Conversely a wide aortic neck was reported in 9; in 2 cases were employed custom-made devices and in 7 standard stent-graft. The use of EVAR with commercially available stent-grafts is feasible and it represents an option especially in non-elective setting

16s rRNA Identification of Pediococcus spp. from Broiler and Studies of Adherence Ability on Immobilized Mucus

The objectives of this research were to study taxonomical status of lactic acid bacteria (LAB) isolated from broiler and adherence ability on mucus in vitro. Molecular analysis was performed by analyzing 16S rRNA gene using universal primer. The adherence assay on mucus was carried out using microplate method with total plate count (TPC), absorbance (A550) and confirmed by scanning electron microscopy (SEM). The results of this studies revealed that three of LAB isolates have closed relation to Pediococcus acidilactici (99.9%) species.Three isolates of P. acidilactici have adherence ability on broiler mucus higher than that on porcine mucin with an adherence percentage of 55.5% versus 50.8% and absorbance A550 of 0.061 versus 0.051, respectively. The highest adherence ability showed by P. acidilactici R02 with adherence percentage was 59.3% and absorbance A550 = 0.068. Adherence on mucus were affected by the addition of 3 g/l of gastric juice and 0.3% (b/v) of bile salt. Adherence analysis using SEM also showed that the adherence on broiler mucus was higher than the adherence on porcine mucin. Altogether this adherence studies, suggest that three isolates of P. acidilactici LAB were capable of colonizing host intestinal mucus in vitro as important property to be promising probiotic bacteria for broiler.Key words : adherence, broiler, Pediococcus, mucus, 16S rRN

PENGUKURAN LUAS METODE TERESTRIS MENGGUNAKAN ALAT UKUR GPS DAN METODE FOTOGRAMETRI MENGGUNAKAN FOTO UDARA UAV DI KOLAM RETENSI MUKTIHARJO KIDUL SEMARANG

Semarang merupakan ibukota Provinsi Jawa Tengah yang perkembangan kotanya sangat pesat. Selain itu Semarang juga merupakan pusat perdagangan dan bisnis, pusat pendidikan dan lain-lain. Di sisi lain karena kepadatan penduduknya tinggi, maka timbul berbagai masalah antara lain terdapat kawasan kumuh, kawasan terdampak genangan banjir air pasang (rob)  dan lain-lain. Kemudian untuk menanggulangi banjir Pemerintah Pusat dan Pemerintah Daerah secara bersama-sama melakukan berbagai pembangunan prasarana fisik antara lain membangun Kolam Retensi yang terletak di wilayah Muktiharjo Kidul. Tujuan pembangunan Kolam Retensi adalah untuk menampung air ketika terjadi hujan besar di sekitar Muktiharjo Kidul, sehingga diharapkan dapat mengurangi banjir di sekitar wilayah kolam retensi. Pembangunan Kolam Retensi Muktiharjo Kidul sangat penting, oleh karena itu perlu dilakukan penelitian luas area yang akan digunakan untuk menghitung kemampuan daya tampung volume kolam retensi. Pengukuran luas dilakukan dengan metode terestris menggunakan alat ukur GPS dan metode fotogrametri menggunakan foto udara UAV. Data hasil perhitungan luas Kolam Retensi dapat digunakan untuk keperluan evaluasi terhadap pembangunan Kolam Retensi Muktiharjo Kidul. Dari hasil pengukuran menggunakan alat ukur GPS metode RTK luas Kolam Retensi sebesar 53.198 m2 , sedangkan dari hasil pengukuran dengan menggunakan data foto udara UAV diperoleh luas sebesar 53.196 m2. Berdasarkan hasil pengukuran tersebut, maka pengukuran luas menggunakan foto udara UAV hasilnya cukup bagus dan mendekati pengukuran dengan alat ukur GP

Metagenomic Analysis of Bacterial Community in the Gut of Blister Beetle Mylabris pustulata Thunberg

Blister beetles are an important object of study in the field of agriculture and health. Despite being known to be important for the host animal, the gut bacteria of blister beetles have not been investigated deeply. This study aimed to provide the first initial description of the gut bacterial community of Mylabris pustulata as one species of blister beetles, based on a culture-independent technique. Adult blister beetles of the same colony were sampled and confirmed as Mylabris pustulata Thunberg. The gut content was used in high throughput sequencing, targeting the V3-V4 regions of 16S rRNA gene, and in the cultivation of resident bacteria. The results showed that higher bacterial richness was present in the gut communities when compared to the grown bacterial culture. Proteobacteria was confirmed as the most abundant phylum in the gut of M. pustulata, whereby most reads belonged to the class of Gammaproteobacteria. The dominant bacterial genera were determined as Enterobacter, Acinetobacter, Enterococcus, Klebsiella, and Pseudomonas. In addition, our cultivation attempts led to successful isolation of members of Klebsiella and Enterococcus. The subject of this study could be explored further to find the potential roles of these microbiota in the gut of the specific beetles and their bioprospects

Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics

The aims of this study were to screen and characterize lactic acid bacteria (LAB) isolated from gastrointestinal (GI) tract of Cemani chicken, one of Indonesian local chicken and to investigate their potential use as probiotics. LAB were isolated from GI tract using MRSA and GYPA media and incubated anaerobically. Selected LAB were determined their probiotic properties with several assays. Identification of selected LAB was based on 16S rDNA sequences, morphological and biochemical characteristics. Ninety five bacteria  were isolated and characterized as lactic acid bacteria (Gram positive, catalase negative, non sporeforming and acid producing). Twenty four isolates of LAB demonstrated antimicrobial activity against Escherichia coli JCM 1649 and Salmonella enteritidis B2586, and three selected isolates, i.e. CCM011, CSP004, and CVM002 showed the highest inhibition activity. The isolates had characters of high cell surface hydrophobicity and inter-isolate coaggregation ability of LAB, high survival at low pH, high  phytase and protease activity (but no amylase and lipase activity), weak coaggregation with pathogen and no resistance to the examined antibiotics. The isolates were identified based on sequence analysis of 16S rRNA gene as Lactobacillus salivarius, however, each isolate had different profiles of sugar fermentation. Therefore the three LAB isolates had potential application as probiotics for chicken.Key words: Cemani chicken, gastrointestinal tract, lactic acid bacteria, probioti

Mechanical rotational thrombectomy in long femoropopliteal artery and stent occlusion in COVID-19 patient: Case report

Introduction: Coronavirus disease-19 (COVID-19) has been increasingly associated with thromboembolic complications. COVID-19 infection has a thrombogenic potential for stents. Herein, we report a case of stent thrombosis in diabetic obese patient COVID-19 positive where was previously released a Multilayer Flow Modulator stent (MFM) for large popliteal aneurysm. Case report: A 78-year-old male was referred to our hospital for fever and acute pain in the left leg. At history, the same patient had endovascular procedure for a large symptomatic popliteal aneurysm, treated through release of three MFM. The pulmonary CT scan showed COVID-19 infection with confirm of rhino-laryngeal swab. Duplex ultrasound and CT-angiography showed complete thrombosis of stents. The treatment consisted of mechanical thrombectomy using an 8Fr catheter Rotarex plus release of Vibahn stent-graft. Discussion: COVID-19 patients can present arterial occlusion. In literature are not reported cases about thrombosis peripheral stent. Minimally invasive approaches in redo-procedure reduce risk of infection. Rotarex device was used in revascularization of acute and subacute iliac and femoropopliteal arteries. The goal is to have a debulking, to avoid an incomplete deployment of stent-graft. In our precedent experience, MFM and stent-graft to treatment of popliteal aneurism were safe. It is important to monitor these patients for early identification of failure and rapprochement. In this case, the COVID-19 infection was determinant in promoting thrombosis. Conclusions: COVID-19 increases risk of thrombosis stent. In our experience debulking through Rotarex and stenting, were decisive factors for revascularization and limb salvage

Endograft connector technique to treat popliteal artery aneurysm in a morbid obese patient.

Surgical repair of popliteal artery aneurysm in morbid obese patients poses additional challenges. We report a morbid obese patient who had a 59mm right popliteal artery aneurysm which was successfully treated with the endograft connector technique. This technique was used to perform the distal anastomosis of the below-knee femoro-popliteal bypass. A 10mm Dacron graft was used as a main graft bypass and an 11 mm/10 cm stentgraft as endograft connector. Following the respective tunnel of the Dacron graft, an end-to-side proximal anastomosis was performed at distal femoral artery. The aneurysm exclusion was obtained through a proximal and a distal ligation. Postoperative duplex showed adequate bypass patency. Knee x-rays demonstrated no signs of stent kinking/fractures. The postoperative course was uneventful and the patient was discharged home on fourth day post operative. The six-month computed tomography scan and the 12-month duplex control showed a patent bypass with no signs of stenosis