350 research outputs found
Copper electroforming service at Laboratorio Subterráneo de Canfranc
Electroforming of copper can be very effective to obtain high radio-purity copper parts for low-background experiments. To support the construction of experiments at the Laboratorio Subterráneo de Canfranc in Spain, a Copper Electroforming Service (CES) set-up is in operation. In this work the electroforming system is described and results on the radio-purity of parts made are presented
RILP regulates vacuolar ATPase through interaction with the V1G1 subunit
Rab-interacting lysosomal protein (RILP) is a downstream effector of the Rab7 GTPase. GTP-bound Rab7 recruits RILP to endosomal membranes and, together, they control late endocytic traffic, phagosome and autophagosome maturation and are responsible for signaling receptor degradation. We have identified, using different approaches, the V1G1 (officially known as ATP6V1G1) subunit of the vacuolar ATPase (V-ATPase) as a RILP-interacting protein. V1G1 is a component of the peripheral stalk and is fundamental for correct V-ATPase assembly. We show here that RILP regulates the recruitment of V1G1 to late endosomal and lysosomal membranes but also controls V1G1 stability. Indeed, we demonstrate that V1G1 can be ubiquitylated and that RILP is responsible for proteasomal degradation of V1G1. Furthermore, we demonstrate that alterations in V1G1 expression levels impair V-ATPase activity. Thus, our data demonstrate for the first time that RILP regulates the activity of the V-ATPase through its interaction with V1G1. Given the importance of V-ATPase in several cellular processes and human diseases, these data suggest that modulation of RILP activity could be used to control V-ATPase function
Highly sensitive gamma-spectrometers of GERDA for material screening: Part I
The GERDA experiment aims to search for the neutrinoless double beta-decay of
76Ge and possibly for other rare processes. The sensitivity of the first phase
is envisioned to be more than one order of magnitude better than in previous
neutrinoless double beta-decay experiments. This implies that materials with
ultra-low radioactive contamination need to be used for the construction of the
detector and its shielding. Therefore the requirements on material screening
include high-sensitivity low-background detection techniques and long
measurement times. In this article, an overview of material-screening
laboratories available to the GERDA collaboration is given, with emphasis on
the gamma-spectrometry. Additionally, results of an intercomparison of the
evaluation accuracy in these laboratories are presented.Comment: Featured in: Proceedings of the XIV International Baksan School
"Particles and Cosmology" Baksan Valley, Kabardino-Balkaria, Russia, April
16-21,2007. INR RAS, Moscow 2008. ISBN 978-5-94274-055-9, pp. 228-232; (5
pages, 0 figures
New experimental limits on the alpha decays of lead isotopes
For the first time a PbWO4 crystal was grown using ancient Roman lead and it
was run as a cryogenic detector. Thanks to the simultaneous and independent
read-out of heat and scintillation light, the detector was able to discriminate
beta/gamma interactions with respect to alpha particles down to low energies.
New more stringent limits on the alpha decays of the lead isotopes are
presented. In particular a limit of T_{1/2} > 1.4*10^20 y at a 90% C.L. was
evaluated for the alpha decay of 204Pb to 200Hg
The Rab-interacting lysosomal protein (RILP) regulates vacuolar ATPase acting on the V1G1 subunit
RILP is a downstream effector of the Rab7 GTPase. GTP-bound Rab7 recruits RILP on endosomal membranes and, together, they control late endocytic traffic, phagosome and autophagosome maturation and are responsible for signaling receptor degradation. We have identified, using different approaches, the V1G1 subunit of the vacuolar ATPase (V-ATPase) as a RILP interacting protein. V1G1 is a component of the peripheral stalk and it is fundamental for correct V-ATPase assembly. We established that RILP regulates the recruitment of V1G1 subunit to late endosomal/lysosomal membranes but also controls V1G1 stability. Indeed, we demonstrated that V1G1 is ubiquitinated and that RILP is responsible for proteasomal degradation of V1G1. Furthermore, we demonstrated that alterations of V1G1 expression levels impair V-ATPase activity. Thus, our data demonstrate for the first time that RILP regulates the activity of the V-ATPase through the interaction with V1G1. Given the importance of V-ATPase in several cellular processes and human diseases, these data suggest that modulation of RILP activity could be used to control V-ATPase function
Discovery of the Eu decay
We report on the first compelling observation of decay of Eu
to the ground state of Pm. The measurement was performed using a 6.15 g
LiEu(BO) crystal operated as a scintillating bolometer. The Q-value
and half-life measured are: Q = 1948.9 keV, and
T y . The
half-life prediction of nuclear theory using the Coulomb and proximity
potential model are in good agreement with this experimental result
RILP regulates vacuolar ATPase through interaction with the V1G1 subunit
Erratum for
RILP regulates vacuolar ATPase through interaction with the V1G1 subunit. [J Cell Sci. 2014
First array of enriched ZnSe bolometers to search for double beta decay
The R&D activity performed during the last years proved the potential of ZnSe
scintillating bolometers to the search for neutrino-less double beta decay,
motivating the realization of the first large-mass experiment based on this
technology: CUPID-0. The isotopic enrichment in Se, the ZnSe
crystals growth, as well as the light detectors production have been
accomplished, and the experiment is now in construction at Laboratori Nazionali
del Gran Sasso (Italy). In this paper we present the results obtained testing
the first three ZnSe crystals operated as scintillating bolometers, and
we prove that their performance in terms of energy resolution, background
rejection capability and intrinsic radio-purity complies with the requirements
of CUPID-0
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