Water-channel study of flow and turbulence past a two-dimensional array of obstacles

A neutral boundary layer was generated in the laboratory to analyze the mean velocity field and the turbulence field within and above an array of two-dimensional obstacles simulating an urban canopy. Different geometrical configurations were considered in order to investigate the main characteristics of the flow as a function of the aspect ratio (AR) of the canopy. To this end, a summary of the two-dimensional fields of the fundamental turbulence parameters is given for AR ranging from 1 to 2. The results show that the flow field depends strongly on AR only within the canyon, while the outer flow seems to be less sensitive to this parameter. This is not true for the vertical momentum flux, which is one of the parameters most affected by AR, both within and outside the canyon. The experiments also indicate that, when (i.e. the skimming flow regime), the roughness sub-layer extends up to a height equal to 1.25 times the height of the obstacles (H), surmounted by an inertial sub-layer that extends up to 2.7 H. In contrast, for (i.e. the wake-interference regime) the inertial sub-layer is not present. This has significant implications when using similarity laws for deriving wind and turbulence profiles in canopy flows. Furthermore, two estimations of the viscous dissipation rate of turbulent kinetic energy of the flow are given. The first one is based on the fluctuating strain rate tensor, while the second is related to the mean strain rate tensor. It is shown that the two expressions give similar results, but the former is more complicated, suggesting that the latter might be used in numerical models with a certain degree of reliability. Finally, the data presented can also be used as a dataset for the validation of numerical models

A street graph-based morphometric characterization of two large urban areas

Urban microclimate modelling, both numerical and in the laboratory, has strong implications in many relevant health and life-style management issues e.g., in studies for assessment and forecast of air quality (for both outdoor and, as boundary conditions, indoor investigations), for thermometric trend analysis in urban zones, in cultural heritage preservation, etc. Moreover, the study of urban microclimate modelling is largely promoted and encouraged by international institutions for its implication in human health protection. In the present work, we propose and discuss an adaptive street graph-based method aimed at automatically computing the geometrical parameters adopted in atmospheric turbulent flow modelling. This method has been applied to two real cases, the Italian cities of Rome and Cagliari, and its results has been compared with the ones from traditional methods based on regular grids. Results show that the proposed method leads to a more accurate determination of the urban canyon parameters (Canyon Aspect Ratio and Building Aspect Ratio) and morphometric parameters (Planar Area Index and Frontal Area Index) compared to traditional regular grid-based methods, at least for the tested cases. Further investigations on a larger number of different urban contexts are planned to thoroughly test and validate the proposed algorithm

BCL-XL inhibition induces an FGFR4-mediated rescue response in colorectal cancer

The heterogeneous therapy response observed in colorectal cancer is in part due to cancer stem cells (CSCs) that resist chemotherapeutic insults. The anti-apoptotic protein BCL-XL plays a critical role in protecting CSCs from cell death, where its inhibition with high doses of BH3 mimetics can induce apoptosis. Here, we screen a compound library for synergy with low-dose BCL-XL inhibitor A-1155463 to identify pathways that regulate sensitivity to BCL-XL inhibition and reveal that fibroblast growth factor receptor (FGFR)4 inhibition effectively sensitizes to A-1155463 both in vitro and in vivo. Mechanistically, we identify a rescue response that is activated upon BCL-XL inhibition and leads to rapid FGF2 secretion and subsequent FGFR4-mediated post-translational stabilization of MCL-1. FGFR4 inhibition prevents MCL-1 upregulation and thereby sensitizes CSCs to BCL-XL inhibition. Altogether, our findings suggest a cell transferable induction of a FGF2/FGFR4 rescue response in CRC that is induced upon BCL-XL inhibition and leads to MCL-1 upregulation

Gestational Diabetes and Thyroid Autoimmunity

Background. About 10% of pregnancies are complicated by previously unknown impairment of glucose metabolism, which is defined as gestational diabetes. There are little data available on prevalence of thyroid disorders in patients affected by gestational diabetes, and about their postgestational thyroid function and autoimmunity. We therefore investigated pancreatic and thyroid autoimmunity in gestational diabetic patients and in women who had had a previous gestational diabetic pregnancy. Methods. We investigated 126 pregnant women at the time of a 100-g oral glucose tolerance test: 91 were classified as gestational diabetics, and 35 were negative (controls). We also studied 69 women who had delivered a baby 18–120 months prior to this investigation and who were classified at that time gestational diabetics (38 women) or normally pregnant (31 women; controls). Results. Our data show no differences for both thyroid function and prevalence of autoimmune disorders during pregnancy; however, a significant increase in thyroid autoimmunity was seen in women previously affected by gestational diabetes. This increased prevalence of thyroid autoimmunity was not associated with the development of impaired glucose metabolism after pregnancy. Conclusions. Our data suggest that maternal hyperglycemia is a risk factor for the development of thyroid autoimmunity, a conclusion that should now be confirmed in a larger cohort of patients

Eleven Years of Health Monitoring in Wild Boars (Sus scrofa) in the Emilia-Romagna Region (Italy)

In recent years, the growth of wild ungulates has increased the focus on their health monitoring. In particular, the health status of wild boars is relevant for the economic impact on the pig industry. The Emilia-Romagna region activated a wildlife monitoring plan to better evaluate the health status of the wild boar population. Between 2011 and 2021, samples of found dead and hunted wild boar have been examined for trichinellosis, tuberculosis, brucellosis, african swine fever, classical swine fever, Aujeszky’s disease, swine vesicular disease, and swine influenza A. Trichinella britovi was identified in 0.001% of the examined wild boars; neither M. bovis nor M. tuberculosis were found in M. tuberculosis complex positive samples; 2.3% were positive for Brucella suis; 29.4% of the sera were positive for Aujeszky’s disease virus; and 0.9% of the samples were positive for swine influenza A virus. With an uncertain population estimate, the number of animals tested, the number of positives, and the sampling method do not allow us to make many inferences but suggest the need to implement and strengthen the existing surveillance activity, as it seems to be the only viable alternative for safeguarding animal and human health

Photosensitization of pancreatic cancer cells by cationic alkyl-porphyrins in free form or engrafted into POPC liposomes: The relationship between delivery mode and mechanism of cell death

Cationic porphyrins bearing an alkyl side chain of 14 (2b) or 18 (2d) carbons dramatically inhibit proliferation of pancreatic cancer cells following treatment with light. We have compared two different ways of delivering porphyrin 2d: either in free form or engrafted into palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine liposomes (L-2d). Cell cytometry shows that while free 2d is taken up by pancreatic cancer cells by active (endocytosis) and passive (membrane fusion) transports, L-2d is internalized solely by endocytosis. Confocal microscopy showed that free 2d co-localizes with the cell membrane and lysosomes, whereas L-2d partly co-localizes with lysosomes and ER. It is found that free 2d inhibits the KRAS-Nrf2-GPX4 axis and strongly triggers lipid peroxidation, resulting in cell death by ferroptosis. By contrast, L-2d does not affect the KRAS-Nrf2-GPX4 axis and activates cell death mainly through apoptosis. Overall, our study demonstrates for the first time that cationic alkyl porphyrins, which have a IC50 ~ 23 nM, activate a dual mechanism of cell death, ferroptosis and apoptosis, where the predominant form depends on the delivery mode

Policy and Practice for Digital Archaeological Archiving in Italy

his article highlights how the Italian Central Institute for Archaeology (ICA) is developing the National Geoportal for Archaeology (GNA), based on the ARIADNEplus infrastructure and its policy framework. Thanks to the GNA project, it will be possible to search and learn about archaeological documentation managed by Superintendencies and Universities holding a significant amount of archaeological data, much of which is either completely or partially unpublished

How healthy is community-dwelling elderly population? Results from southern Italy

PURPOSE To explore the frequency of polypharmacy, functional and cognitive capacity among the elderly in Southern Italy. METHODS Populationbased retrospective cross-sectional study. Information were retrieved from electronic-geriatric-forms matched by record-linkage to outpatient pharmacy-records. The following domains were collected from geriatric forms: BMI, cognitive capacity (SPMSQ), functional status Barthel-index), mobility, living condition. Polypharmacy status was categorized as non-polypharmacy (0-4), polypharmacy (5-9) and excessive-polypharmacy (>10). Prevalence of all variables were stratified by age and polypharmacy group. RESULTS 88,878 old people received a geriatric assessment in the years 2013-2014. Mean age was 74.8 (±7.3) years, 56.6% females. Proportion of elderly in excessive-polypharmacy increased with age (18.9% in 65-75 age-group; 27.9% in >85). Referring to cognitive capacity, the proportion of lucid patients decreased with age (from 94.3% to 58.1%), while confused patient increased with age (from 4.7% to 30.9%). Proportion of subjects with a decline in cognitive status, functional status and mobility increased in polypharmacy and excessive polypharmacy group. CONCLUSION Polypharmacy is common in people aged 65 years and older with difficulties in activities of daily living and impaired cognition. Furthermore, its prevalence raises with increasing age. Preventive strategies such us optimization of drug regimen should be performed routinely to reduce risk of adverse-health-events