Field sampling of soil pore water to evaluate the mobile fraction of trace elements in the Iglesiente area (SW Sardinia, Italy)

Field soil pore water monitoring was applied in a highly heavy-metal contaminated area in SW Sardinia, Italy, as a direct, realistic measure of heavy metal mobility. The main chemistry of pore waters well reflects the local characteristics of soils, ranging from Ca-SO4 to (Ca)Mg-HCO3 to Ca(Na)-SO4(Cl), with a wide range of conductivity. The mobility of Zn and Pb is apparently controlled by equilibrium with minerals such as hydrozincite or smithsonite, and cerussite, respectively. These results allow a correct estimate of the actual environmental risk associated with the presence of heavy metals in soils, and may serve as a supporting tool for phytoremediation planning

Assembly of the Auditory Circuitry by a Hox Genetic Network in the Mouse Brainstem

Rhombomeres (r) contribute to brainstem auditory nuclei during development. Hox genes are determinants of rhombomere-derived fate and neuronal connectivity. Little is known about the contribution of individual rhombomeres and their associated Hox codes to auditory sensorimotor circuitry. Here, we show that r4 contributes to functionally linked sensory and motor components, including the ventral nucleus of lateral lemniscus, posterior ventral cochlear nuclei (VCN), and motor olivocochlear neurons. Assembly of the r4-derived auditory components is involved in sound perception and depends on regulatory interactions between Hoxb1 and Hoxb2. Indeed, in Hoxb1 and Hoxb2 mutant mice the transmission of low-level auditory stimuli is lost, resulting in hearing impairments. On the other hand, Hoxa2 regulates the Rig1 axon guidance receptor and controls contralateral projections from the anterior VCN to the medial nucleus of the trapezoid body, a circuit involved in sound localization. Thus, individual rhombomeres and their associated Hox codes control the assembly of distinct functionally segregated sub-circuits in the developing auditory brainstem

Spermatozoa transcriptional response and alterations in pl proteins properties after exposure of mytilus galloprovincialis to mercury

Mercury (Hg) is an environmental pollutant that impacts human and ecosystem health. In our previous works, we reported alterations in the properties of Mytilus galloprovincialis protamine-like (PL) proteins after 24 h of exposure to subtoxic doses of toxic metals such as copper and cadmium. The present work aims to assess the effects of 24 h of exposure to 1, 10, and 100 pM HgCl2 on spermatozoa and PL proteins of Mytilus galloprovincialis. Inductively coupled plasma–mass spectrometry indicated accumulation of this metal in the gonads of exposed mussels. Further, RT-qPCR analyses showed altered expression levels of spermatozoa mt10 and hsp70 genes. In Mytilus galloprovincialis, PL proteins represent the major basic component of sperm chromatin. These proteins, following exposure of mussels to HgCl2, appeared, by SDS-PAGE, partly as aggregates and showed a decreased DNA-binding capacity that rendered them unable to prevent DNA damage, in the presence of CuCl2 and H2O2 . These results demonstrate that even these doses of HgCl2 exposure could affect the properties of PL proteins and result in adverse effects on the reproductive system of this organism. These analyses could be useful in developing rapid and efficient chromatin-based genotoxicity assays for pollution biomonitoring programs

Acne radar: A new intuitive graphic visualization of quality of life in acne patients

Background: There are many instruments available in literature to evaluate the influence of acne on Qualify of Life (QoL), but many of these questionnaires rarely are performed in daily clinical practice. Many of these instruments require a long time for the compilation, and often they are not self completed. There are no simple and intuitive instrument that can be completed by patients alone, with a graphical representation of the results. Objective: To develop a new, rapid and intuitive graphic instrument to a questionnaire allowing both a quick assessment of the impact of acne on patients and, at the same time, increase patient's compliance and self-esteem. Methods: In our study 50 patients were recruited and questionnaire was administered following the main items evaluated in the validated index of QoL from previous studies for acne patients. We have applied an intuitive graph representation, the "Radar Graph", and other statistical methods like the Decisional Map and the Principal Component Analysis, to this questionnaire allowing a quick assessment of the impact of acne on patients. Results: The questionnaire evaluated have 10 items regarding 3 different area: the objective symptoms such as negative perception of their image (imperfection), sting and insomnia; the subjective symptoms such as depression, perception of illness, lack of serenity and shame; and the relational difficulties such as social relationships, working relationships, and intimate relations. The answers are given on a 10-point visual analogue scale. Conclusion: Our survey can be a new, rapid and intuitive graphic instrument to use in clinical practice

Examining ecosystem services and disservices through deliberative socio-cultural valuation

The deliberative socio-cultural valuation of ecosystem services (ES) and disservices (EDS) is an understudied area of ES and EDS research. Participatory methods have been applied to ES and EDS valuation, but little is known on how these approaches could reveal and form shared values and impact decision-making. This paper presents the deliberative socio-cultural valuation of the Jose Rizal Plaza in Calamba City, The Philippines. The study aimed to assess how stakeholders value the ES and EDS of the park and examine how these values change in different situations. Online focus groups were carried out, and in each, the participants were asked to distribute importance and concern points to the various park ES and EDS, respectively. The valuation exercise was performed six times, changing the source and constituency of the valuation, and introducing discussions. Results confirm significant differences in the values assigned to several ES and EDS across the valuation exercises. Varying the sources and constituencies proved useful in revealing the participants’ shared assigned values. The participants share a high appreciation for enjoyment and spending free time, sports and physical fitness, relaxation and mental recreation, social relationships, and local identity and cultural heritage. For EDS, they share a significant concern only for the risk of anti-social behaviour. This type of valuation could be further explored using other parks and cities to test if it will have consistent results. For the Jose Rizal Plaza, spaces for sports should be maintained and security should be improved

Socio-cultural valuation of urban parks: the case of Jose Rizal Plaza in Calamba City, The Philippines

The socio-cultural value of urban parks has rarely been studied, and this could be why they are undervalued and not given significant attention in city planning. This study presents the socio-cultural valuation (SCV) of the ecosystem services (ES) and disservices (EDS) of the Jose Rizal Plaza in Calamba City, The Philippines. Stakeholders were interviewed to assemble a list of the park's ES and EDS. An online survey was then conducted to examine how stakeholders assign values to the park ES and EDS. Finally, the configuration of conditions leading to the assigned values was analysed. The results suggest that respondents value the park's ES more than they worry about its EDS. They value cultural ES the highest, while they are most concerned with psychological EDS. The Fuzzy-set Qualitative Comparative Analysis (fsQCA) revealed that visiting the park is necessary for a high valuation to ES. For EDS, these are: not knowing the previous land use in the area and visiting the park. Overall, the paper concludes that SCV is an effective way to assess the value of urban parks, and fsQCA could aid in determining the combination of conditions leading to these values

Is Metabolic Syndrome Useful for Identifying Youths with Obesity at Risk for NAFLD?

The definition of metabolic syndrome (MetS) in childhood is controversial. Recently, a modified version of the International Diabetes Federation (IDF) definition was proposed using reference data from an international population for high waist circumference (WC) and blood pressure (BP), while the fixed cutoffs for lipids and glucose were not changed. We analyzed MetS prevalence using this modified definition (MetS-IDFm) and its association with non-alcoholic fatty liver disease (NAFLD) in 1057 youths (age 6–17 years) with overweight/obesity (OW/OB). A comparison with another modified definition of MetS according to the Adult Treatment Panel III (MetS-ATPIIIm) was performed. The prevalence of MetS-IDFm was 27.8% and 28.9% by MetS-ATPIIIm. The Odds (95% Confidence Intervals) of NAFLD was 2.70 (1.30–5.60) (p = 0.008) for high WC, 1.68 (1.25–2.26)(p = 0.001) for MetS, 1.54 (1.12–2.11)(p = 0.007) for low HDL-Cholesterol, 1.49 (1.04–2.13)(p = 0.032) for high triglycerides and 1.37 (1.03–1.82)(p = 0.033) for high BP. No substantial difference was found in the prevalence of MetS-IDFm and frequency of NAFLD compared to Mets-ATPIIIm definition. Our data demonstrate that one third of youths with OW/OB have MetS, whichever was the criterion. Neither definition was superior to some of their components in identifying youths with OW/OB at risk for NAFLD

Geogenic versus anthropogenic behaviour and geochemical footprint of Al, Na, K and P in the Campania region (Southern Italy) soils through compositional data analysis and enrichment factor

Geochemical studies that focus on environmental applications tend to approach the chemical elements as individual entities and may therefore offer only partial and sometimes biased interpretations of their distributions and behaviour. A potential alternative approach is to consider a compositional data analysis, where every element is part of a whole. In this study, an integrated methodology, which included compositional data analysis, multifractal data transformations and interpolation, as well as enrichment factor analysis, was applied to a geochemical dataset for the Campania region, in the south of Italy, focusing in particular on the behaviour, footprints and sources of a smaller pool of elements: Al, Na, K and P. The initial dataset included 3669 topsoil samples, collected at an average sampling density of 1 site per 2.3 km2, and analyzed (after an aqua regia extraction) by a combination of ICP-AES and ICP-MS for 53 elements. Frequency based methods (Clr biplot, Enrichment Factor computation) and frequency spatial-method (fractal and multifractal plots) allowed identifying the relationships between the elements and their possible source patterns in Campania soils in relation to a natural occurring concentrations in geogenic material (rocks, soils and sediments) or human input. Results showed how the interpretation of concentration and behaviour of Al, Na, K and P was enhanced thanks to the application of data log-ratio transformation in univariate and multivariate analysis compared to the use of raw or log-normal data. Multivariate analyses with compositional biplot allowed the identification of four element associations and their potential association with the underling geology and/or human activities. When focusing on the smaller pool of elements (Al, P, K and Na), these relationships with the unique geology of the region, were largely confirmed by multifractal interpolated maps. However, when the local background was used for the calculation of the enrichment factor, the resulting interpolated maps allowed to identify smaller areas where the greater concentrations of P could not be possibly associated to a mineralisation (e.g., ultrapotassic rocks) but were more likely to be associated to anthropogenic input such as agriculture activities with potentially extensive use of phosphate fertilizers. The integrated approach of this study allowed a more robust qualitative and quantitative evaluation of elemental concentration, providing in particular new and vital information on the distribution and patterns of P in soils of the Campania region, but also a viable, more robust, methodological approach to regional environmental geochemistry studies

Elevated blood pressure, cardiometabolic risk and target organ damage in youth with overweight and obesity

Background and aim: To compare cardiometabolic risk profile and preclinical signs of target organ damage in youth with normal and elevated blood pressure (BP), according to the American Academy of Pediatrics (AAP) guidelines. Methods and results: This cross-sectional multicenter study included 2739 youth (5-17 year-old; 170 normal-weight, 610 overweight and 1959 with obesity) defined non hypertensive by the AAP guidelines. Anthropometric, biochemical and liver ultrasound data were available in the whole population; carotid artery ultrasound and echocardiographic assessments were available respectively in 427 and 264 youth. Elevated BP was defined as BP 65 90th to <95th percentile for age, gender and height in children or BP 65 120/80 to <130/80 in adolescents. The overall prevalence of elevated BP was 18.3%, and significantly increased from normal-weight to obese youth. Young people with elevated BP showed higher levels of body mass index (BMI), insulin resistance and a higher prevalence of liver steatosis (45% vs 36%, p < 0.0001) than normotensive youth, whilst they did not differ for the other cardiometabolic risk factors, neither for carotid intima media thickness or left ventricular mass. Compared with normotensive youth, individuals with elevated BP had an odds ratio (95%Cl) of 3.60 (2.00\u20136.46) for overweight/obesity, 1.46 (1.19\u20131.78) for insulin-resistance and 1.45 (1.19\u20131.77) for liver steatosis, controlling for centers, age and prepubertal stage. The odds for insulin resistance and liver steatosis persisted elevated after correction for BMI-SDS. Conclusion: Compared to normotensive youth, elevated BP is associated with increased BMI, insulin resistance and liver steatosis, without significant target organ damage

The peopling of the last Green Sahara revealed by high-coverage resequencing of trans-Saharan patrilineages

Little is known about the peopling of the Sahara during the Holocene climatic optimum, when the desert was replaced by a fertile environment