Propiedades psicométricas del cuestionario de agresividad de Buss y Perry AQ, en internos del establecimiento penitenciario de Cambio Puente - Chimbote

El objetivo de la investigación fue determinar las Propiedades Psicométricas del Cuestionario de Agresividad de Buss y Perry AQ, en Internos Del Establecimiento Penitenciario De Cambio Puente – Chimbote. El tipo de estudio fue instrumental y el diseño de investigación psicométrico. La muestra estuvo conformada por 344 Internos Del Establecimiento Penitenciario De Cambio Puente, todos varones. La evidencia de validez basada en la estructura interna fue realizada por el análisis factorial confirmatorio, reporta para el absoluto, GFI>,909 , AGFI>,917 , CFI> ,963 , IFI> ,919, que indican un buen ajuste, todo ello se calculó con ayuda del software estadístico AMOS 26. Además, se calculó a través del software estadístico r el coeficiente de fiabilidad Omega (McDonald, 1999) para estimar de forma puntual e interválica, al 95% de confianza la consistencia interna del instrumento, considerando valores mayores a .70 como aceptables (Campo-Arias y Oviedo, 2008)

Large differences in adiponectin levels have no clear effect on multiple sclerosis risk: A Mendelian randomization study.

BACKGROUND: Mendelian randomization (MR) studies have demonstrated strong support for an association between genetically increased body mass index and risk of multiple sclerosis (MS). The adipokine adiponectin may be a potential mechanism linking body mass to risk of MS. OBJECTIVE: To evaluate whether genetically increased adiponectin levels influence risk of MS. METHODS: Using genome-wide significant single nucleotide polymorphisms (SNPs) for adiponectin, we undertook an MR study to estimate the effect of adiponectin on MS. This method prevents bias due to reverse causation and minimizes bias due to confounding. Sensitivity analyses were performed to evaluate the assumptions of MR. RESULTS: MR analyses did not support a role for genetically elevated adiponectin in risk of MS (odds ratio (OR) = 0.93 per unit increase in natural-log-transformed adiponectin, equivalent to a two-standard deviation increase in adiponectin on the absolute scale; 95% confidence interval (CI) = 0.66-1.33; p = 0.61). Further MR analysis suggested that genetic variation at the adiponectin gene, which influences adiponectin level, does not impact MS risk. Sensitivity analyses, including MR-Egger regression, suggested no bias due to pleiotropy. CONCLUSION: Lifelong genetically increased adiponectin levels in humans have no clear effect on risk of MS. Other biological factors driving the association between body mass and MS should be investigated

The mouse cortical meninges are the site of immune responses to many different pathogens, and are accessible to intravital imaging

A wide range of viral and microbial infections are known to cause meningitis, and there is evidence that the meninges are the gateway to pathogenic invasion of the brain parenchyma. Hence observation of these regions has wide application to understanding host-pathogen interactions. Interactions between pathogens and cells of the immune response can be modified by changes in their environment, such as suppression of the flow of blood and lymph, and, particularly in the case of the meninges, with their unsupported membranes, invasive dissection can alter the tissue architecture. For these reasons, intravital imaging through the unperforated skull is the method of choice. We give a protocol for a simple method of two-photon microscopy through the thinned cortical skull of the anesthetized mouse to enable real-time imaging with sub-micron resolution through the meninges and into the superficial brain parenchyma. In reporter mice in which selected cell types express fluorescent proteins, imaging after infection with fluorescent pathogens (lymphocytic choriomeningitis virus, Trypanosoma brucei or Plasmodium berghei) has shown strong recruitment to the cortical meninges of immune cells, including neutrophils, T cells, and putative dendritic cells and macrophages. Without special labeling, the boundaries between the dura mater, the leptomeninx, and the parenchyma are not directly visualized in intravital two-photon microscopy, but other landmarks and characteristics, which we illustrate, allow the researcher to identify the compartment being imaged. While most infectious meningitides are localized mainly in the dura mater, others involve recruitment of immune cells to the leptomeninx

Organizational analysis of energy manpower requirements in the United States navy

The Secretary of the Navy (SECNAV) directed NPS to establish energy-focused subspecialty codes (SSC) that will prepare officers to manage all aspects of energy. In response to this SECNAV directive NPS has developed four energy-focused degree plans in the areas of Operations Analysis, Financial Management, Mechanical and Electrical Engineering. An analysis of the current force structure requirements was necessary to assess and implement a new direction. At the present time, the Navy utilizes petroleum management officers as energy managers. Unfortunately, the Navy Officer Billet Classification (NOBC) Codes assigned to these officers do not translate into the identification of the billets being identified with the energy SSCs. Analysis shows a possible solution to this issue is to establish afloat and ashore general Energy NOBCs that could be assigned as either a primary or secondary NOBC Energy billets. Specifically, analysis shows the majority of NOBCs assigned to energy billets are from the Naval Operations (90009999) Field. Therefore, this research recommends the establishment of two Energy NOBCs to support future Fleet Energy Management Challenges.http://archive.org/details/organizationalna1094534655Lieutenant Junior Grade, United States NavyApproved for public release; distribution is unlimited

Increased doublecortin (DCX) expression and incidence of DCX-immunoreactive multipolar cells in the subventricular zone-olfactory bulb system of suicides

Postmortem studies have confirmed the occurrence of adult hippocampal neurogenesis in humans and implicated this process in antidepressant response, yet neurogenesis in other regions remains to be examined in the context of depression. Here we assess the extent of subventricular zone-olfactory bulb (SVZ-OB) neurogenesis in adult humans having died by suicide. Protein expression of proliferative and neurogenic markers Sox2, proliferating cell nuclear antigen, and doublecortin (DCX) were examined in postmortem SVZ and OB samples from depressed suicides and matched sudden-death controls. In the SVZ, DCX-immunoreactive (IR) cells displayed phenotypes typical of progenitors, whereas in the olfactory tract (OT), they were multipolar with variable size and morphologies suggestive of differentiating cells. DCX expression was significantly increased in the OB of suicides, whereas SVZ DCX expression was higher among unmedicated, but not antidepressant-treated, suicides. Although very few DCX-IR cells were present in the control OT, they were considerably more common in suicides and correlated with OB DCX levels. Suicides also displayed higher DCX-IR process volumes. These results support the notion that OB neurogenesis is minimal in adult humans. They further raise the possibility that the differentiation and migration of SVZ-derived neuroblasts may be altered in unmedicated suicides, leading to an accumulation of ectopically differentiating cells in the OT. Normal SVZ DCX expression among suicides receiving antidepressants suggests a potentially novel mode of action of antidepressant medication. Given the modest group sizes and rarity of DCX-IR cells assessed here, a larger-scale characterization will be required before firm conclusions can be made regarding the identity of these cells

Induction of recurrent break cluster genes in neural progenitor cells differentiated from embryonic stem cells in culture

Mild replication stress enhances appearance of dozens of robust recurrent genomic break clusters, termed RDCs, in cultured primary mouse neural stem and progenitor cells (NSPCs). Robust RDCs occur within genes (“RDC-genes”) that are long and have roles in neural cell communications and/or have been implicated in neuropsychiatric diseases or cancer. We sought to develop an in vitro approach to determine whether specific RDC formation is associated with neural development. For this purpose, we adapted a system to induce neural progenitor cell (NPC) development from mouse embryonic stem cell (ESC) lines deficient for XRCC4 plus p53, a genotype that enhances DNA double-strand break (DSB) persistence to enhance detection. We tested for RDCs by our genome-wide DSB identification approach that captures DSBs via their ability to join to specific genomic Cas9/single-guide RNA–generated bait DSBs. In XRCC4/p53-deficient ESCs, we detected seven RDCs, all of which were in genes and two of which were robust. In contrast, in NPCs derived from these ESC lines we detected 29 RDCs, a large fraction of which were robust and associated with long, transcribed neural genes that were also robust RDC-genes in primary NSPCs. These studies suggest that many RDCs present in NSPCs are developmentally influenced to occur in this cell type and indicate that induced development of NPCs from ESCs provides an approach to rapidly elucidate mechanistic aspects of NPC RDC formation.</jats:p